Standard name | |
---|---|
Human Ortholog | |
Description | Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 20 | 15 | 20 | 38 | 86 | 98 | 108 | – | 84 | 114 | 89 | 14 | 4 | 5 | 24 | 6 | 4 | 0 | 2 | 1 | 5 | 5 | 5 |
Bud | 2 | 8 | 2 | 3 | 5 | 6 | 11 | 13 | – | 9 | 11 | 5 | 0 | 0 | 1 | 9 | 9 | 3 | 0 | 3 | 3 | 5 | 1 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 75 | 199 | 215 | 152 | 154 | 174 | 165 | 180 | – | 156 | 175 | 137 | 458 | 300 | 286 | 339 | 289 | 254 | 87 | 289 | 262 | 287 | 323 | 387 |
Cytoplasm | 8 | 29 | 7 | 10 | 15 | 21 | 24 | 22 | – | 18 | 32 | 18 | 15 | 13 | 15 | 83 | 45 | 38 | 3 | 18 | 2 | 8 | 4 | 6 |
Endoplasmic Reticulum | 3 | 8 | 2 | 7 | 15 | 4 | 8 | 22 | – | 11 | 17 | 15 | 39 | 35 | 21 | 13 | 6 | 20 | 11 | 12 | 0 | 40 | 20 | 21 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2 | 13 | 7 | 14 | – | 21 | 32 | 24 | 0 | 0 | 0 | 21 | 13 | 14 | 1 | 8 | 7 | 8 | 5 | 11 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | – | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 2 | 0 | 1 | 0 | 0 |
Unique Cell Count | 82 | 225 | 225 | 170 | 184 | 210 | 203 | 253 | 187 | 230 | 176 | 511 | 348 | 320 | 402 | 330 | 288 | 105 | 341 | 281 | 363 | 366 | 449 | |
Labelled Cell Count | 96 | 264 | 243 | 193 | 236 | 307 | 317 | 372 | 299 | 384 | 288 | 530 | 357 | 331 | 490 | 373 | 336 | 105 | 341 | 281 | 363 | 366 | 449 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 41.1 | 58.3 | 55.3 | 47.7 | 35.2 | 31.0 | 30.4 | 31.9 | – | 32.4 | 31.2 | 31.7 | 75.7 | 79.7 | 81.7 | 41.6 | 52.6 | 59.9 | 39.8 | 41.5 | 46.6 |
Std Deviation (1e-4) | 9.7 | 15.1 | 12.7 | 12.0 | 12.5 | 11.6 | 11.9 | 10.6 | – | 11.2 | 11.2 | 10.3 | 18.2 | 20.2 | 19.5 | 14.0 | 16.3 | 19.8 | 11.2 | 12.5 | 13.3 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.65 | -0.84 | -0.86 | -0.79 | – | -0.77 | -0.83 | -0.8 | 0.45 | 0.53 | 0.56 | -0.41 | -0.07 | 0.11 | -0.48 | -0.41 | -0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.8 | 4.2 | 8.5 | 9.8 | 9.0 | – | 9.0 | 10.2 | 10.0 | -2.5 | -3.6 | -3.1 | -0.3 | -2.9 | -3.1 |
Bud | 0 | 0 | 0 | 2.7 | 2.7 | – | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.4 | -4.0 | -4.3 | -4.7 | -7.0 | – | -4.1 | -5.9 | -5.4 | -2.6 | -3.6 | -2.6 | -4.2 | -3.2 | -3.0 |
Cytoplasm | 1.3 | 2.2 | 2.9 | 3.5 | 2.6 | – | 2.8 | 4.1 | 2.9 | -0.1 | 0.4 | 0.9 | 6.0 | 4.2 | 4.0 |
Endoplasmic Reticulum | 0 | 3.7 | 0 | 0 | 3.9 | – | 2.9 | 3.5 | 3.8 | 3.7 | 4.4 | 3.2 | 1.8 | 0 | 3.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.8 | 0 | 3.6 | – | 5.2 | 5.8 | 5.7 | 0 | 0 | 0 | 3.5 | 3.0 | 3.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 58.6619 | 86.2924 | 79.048 | 69.4903 | 68.8493 | 76.483 | 46.2354 | 63.8785 | 56.0166 | 54.0674 | 54.561 | 58.0218 | 91.1106 | 109.6637 | 95.8975 | 95.2188 | 91.9875 | 99.3925 |
Actin | 0.0155 | 0.0001 | 0.0005 | 0.0032 | 0.0046 | 0.0063 | 0.0426 | 0.0004 | 0.0139 | 0.0214 | 0.0749 | 0.0021 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.002 |
Bud | 0.0014 | 0.0001 | 0.0003 | 0.0004 | 0.0009 | 0.0005 | 0.0011 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.0041 | 0.0003 | 0.0003 | 0.0002 | 0.0024 | 0.0017 | 0.0045 | 0.0003 | 0.0005 | 0.0052 | 0.0007 | 0.0033 | 0.0011 | 0.0002 | 0.0013 | 0.0012 | 0.0026 | 0.0106 |
Bud Periphery | 0.0036 | 0.0003 | 0.0008 | 0.0007 | 0.0022 | 0.0021 | 0.0054 | 0.0003 | 0.0013 | 0.0007 | 0.0006 | 0.0021 | 0.0016 | 0.0002 | 0.0006 | 0.0011 | 0.0009 | 0.0039 |
Bud Site | 0.0075 | 0.0005 | 0.0006 | 0.0008 | 0.0022 | 0.0004 | 0.0081 | 0.0017 | 0.0109 | 0.0084 | 0.0003 | 0.0005 | 0.0053 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Cell Periphery | 0.3769 | 0.5313 | 0.4457 | 0.373 | 0.4074 | 0.4162 | 0.25 | 0.354 | 0.2895 | 0.2393 | 0.2475 | 0.2277 | 0.4736 | 0.6017 | 0.5453 | 0.5563 | 0.4536 | 0.4674 |
Cytoplasm | 0.0628 | 0.0809 | 0.068 | 0.0154 | 0.0765 | 0.062 | 0.0135 | 0.0212 | 0.0162 | 0.0096 | 0.0014 | 0.0207 | 0.0085 | 0.0158 | 0.0161 | 0.0043 | 0.0732 | 0.0593 |
Cytoplasmic Foci | 0.0253 | 0.0008 | 0.0038 | 0.0403 | 0.0115 | 0.0028 | 0.0129 | 0 | 0.0031 | 0.0037 | 0.0048 | 0.0023 | 0.0045 | 0 | 0 | 0 | 0 | 0.0001 |
Eisosomes | 0.0028 | 0.0001 | 0.0003 | 0.0004 | 0.0019 | 0.0039 | 0.0028 | 0.0004 | 0.0012 | 0.0003 | 0.0003 | 0.0005 | 0.0012 | 0.0005 | 0.0004 | 0.0011 | 0.0001 | 0.0005 |
Endoplasmic Reticulum | 0.2609 | 0.2414 | 0.3266 | 0.3444 | 0.2619 | 0.3163 | 0.5244 | 0.5439 | 0.5852 | 0.6269 | 0.584 | 0.6621 | 0.3038 | 0.1787 | 0.2321 | 0.1702 | 0.2992 | 0.2703 |
Endosome | 0.0143 | 0.0001 | 0.0008 | 0.0052 | 0.0089 | 0.0114 | 0.0109 | 0 | 0.0006 | 0.0018 | 0.0016 | 0.0006 | 0.0013 | 0 | 0 | 0 | 0 | 0.0001 |
Golgi | 0.0036 | 0 | 0.0002 | 0.0027 | 0.0019 | 0.0039 | 0.0045 | 0 | 0.0012 | 0.0032 | 0.0038 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Lipid Particles | 0.0237 | 0.0143 | 0.011 | 0.0313 | 0.0124 | 0.0149 | 0.0106 | 0.0053 | 0.0048 | 0.0078 | 0.0051 | 0.0044 | 0.0065 | 0.0054 | 0.0047 | 0.0034 | 0.0042 | 0.0084 |
Mitochondria | 0.0128 | 0.0001 | 0.014 | 0.0137 | 0.0402 | 0.0144 | 0.007 | 0.0002 | 0.0012 | 0.0008 | 0.0002 | 0.0024 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
None | 0.0247 | 0.0016 | 0.0016 | 0.0091 | 0.0194 | 0.0014 | 0.0023 | 0.0001 | 0.0049 | 0.0001 | 0.0003 | 0.0027 | 0.0049 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0502 | 0.0401 | 0.059 | 0.0468 | 0.039 | 0.0374 | 0.0421 | 0.042 | 0.0359 | 0.0397 | 0.0496 | 0.032 | 0.1012 | 0.1076 | 0.1197 | 0.1532 | 0.0692 | 0.082 |
Nucleolus | 0.0057 | 0.0003 | 0.0014 | 0.0025 | 0.0039 | 0.0005 | 0.0038 | 0.0005 | 0.0016 | 0.0001 | 0 | 0.0008 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 |
Nucleus | 0.0071 | 0.0006 | 0.0011 | 0.002 | 0.0017 | 0.0016 | 0.0115 | 0.0028 | 0.0011 | 0.0003 | 0.0002 | 0.0012 | 0.001 | 0.0003 | 0.0005 | 0.0008 | 0.0008 | 0.0017 |
Peroxisomes | 0.0027 | 0 | 0.0012 | 0.0034 | 0.0019 | 0.0052 | 0.0071 | 0 | 0.0003 | 0.0069 | 0.0073 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0073 | 0 | 0.0002 | 0.0132 | 0.0009 | 0.0014 | 0.0041 | 0 | 0.0018 | 0.0002 | 0.0018 | 0.0107 | 0.0011 | 0 | 0 | 0 | 0 | 0.0001 |
Vacuole | 0.0823 | 0.0872 | 0.0617 | 0.0838 | 0.0967 | 0.094 | 0.0294 | 0.0265 | 0.0243 | 0.023 | 0.0154 | 0.0221 | 0.0818 | 0.0889 | 0.0784 | 0.1068 | 0.0949 | 0.0918 |
Vacuole Periphery | 0.0047 | 0.0001 | 0.0009 | 0.0076 | 0.0014 | 0.0016 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0006 | 0.0007 | 0.0002 | 0.0005 | 0.0011 | 0.0008 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 193.1391 | 485.7038 | 711.2511 | 634.2111 | 214.8538 | 362.9813 | 740.5561 | 773.8664 | 758.72 | 742.0451 |
Translational Efficiency | 0.9441 | 0.9909 | 1.0856 | 0.9534 | 1.0633 | 0.7793 | 0.9315 | 1.025 | 0.9921 | 0.9191 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
112 | 1167 | 117 | 1444 | 873 | 1897 | 903 | 26 | 985 | 3064 | 1020 | 1470 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2275.35 | 4516.64 | 6989.77 | 4636.82 | 9190.48 | 3887.67 | 7289.96 | 3893.07 | 8404.19 | 4127.23 | 7255.53 | 4623.67 |
Standard Deviation | 983.13 | 1337.31 | 1736.43 | 1679.81 | 2265.87 | 1016.11 | 1815.29 | 1549.26 | 3078.85 | 1188.98 | 1808.95 | 1680.45 |
Intensity Change Log 2 | — | 0.989161 | 1.619157 | 1.027047 | — | -1.241234 | -0.334229 | -1.239232 | — | -0.448140 | 0.316628 | -0.426742 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001790 | 0.001069 | 0.000402 | 0.003057 | 0.000241 | 0.000634 | 0.000347 | 0.002366 | 0.000417 | 0.000800 | 0.000353 | 0.003045 |
Bud Neck | 0.003653 | 0.001622 | 0.002104 | 0.001491 | 0.000507 | 0.000721 | 0.000425 | 0.001361 | 0.000864 | 0.001064 | 0.000617 | 0.001489 |
Bud Site | 0.023545 | 0.019310 | 0.001573 | 0.030552 | 0.003985 | 0.005939 | 0.001798 | 0.045258 | 0.006209 | 0.011031 | 0.001772 | 0.030812 |
Cell Periphery | 0.706066* | 0.859220* | 0.778368* | 0.882369* | 0.943701* | 0.896025* | 0.974191* | 0.933208* | 0.916681* | 0.882007* | 0.951729* | 0.883268* |
Cytoplasm | 0.036442 | 0.029989 | 0.000112 | 0.040704 | 0.004521 | 0.001756 | 0.000173 | 0.000244 | 0.008151 | 0.012509 | 0.000166 | 0.039989 |
Cytoplasmic Foci | 0.004559 | 0.000433 | 0.000108 | 0.000838 | 0.000182 | 0.000435 | 0.001045 | 0.000074 | 0.000680 | 0.000434 | 0.000938 | 0.000824 |
Eisosomes | 0.001093 | 0.003616 | 0.007372 | 0.000926 | 0.002145 | 0.001760 | 0.003524 | 0.004080 | 0.002026 | 0.002467 | 0.003965 | 0.000982 |
Endoplasmic Reticulum | 0.002989 | 0.002657 | 0.002174 | 0.000762 | 0.000298 | 0.010679 | 0.000216 | 0.000232 | 0.000604 | 0.007623 | 0.000440 | 0.000753 |
Endosome | 0.007272 | 0.000488 | 0.000265 | 0.000599 | 0.000132 | 0.000751 | 0.000292 | 0.000072 | 0.000943 | 0.000651 | 0.000289 | 0.000590 |
Golgi | 0.014816 | 0.002263 | 0.002081 | 0.001507 | 0.001083 | 0.003095 | 0.000286 | 0.000167 | 0.002645 | 0.002778 | 0.000492 | 0.001483 |
Lipid Particles | 0.003908 | 0.005517 | 0.001385 | 0.002425 | 0.000320 | 0.001890 | 0.000026 | 0.000027 | 0.000728 | 0.003271 | 0.000182 | 0.002383 |
Mitochondria | 0.000785 | 0.000524 | 0.000215 | 0.001462 | 0.000227 | 0.000466 | 0.000633 | 0.000383 | 0.000291 | 0.000488 | 0.000586 | 0.001443 |
Mitotic Spindle | 0.000485 | 0.000929 | 0.001381 | 0.001077 | 0.000299 | 0.001238 | 0.000205 | 0.000270 | 0.000320 | 0.001120 | 0.000340 | 0.001063 |
None | 0.000029 | 0.000253 | 0.000036 | 0.000134 | 0.000040 | 0.000039 | 0.000015 | 0.000017 | 0.000039 | 0.000121 | 0.000018 | 0.000132 |
Nuclear Periphery | 0.000726 | 0.003587 | 0.007221 | 0.000967 | 0.000485 | 0.013947 | 0.000350 | 0.000237 | 0.000513 | 0.010001 | 0.001138 | 0.000954 |
Nuclear Periphery Foci | 0.000924 | 0.000914 | 0.000306 | 0.000323 | 0.000140 | 0.000462 | 0.000034 | 0.000045 | 0.000229 | 0.000634 | 0.000065 | 0.000318 |
Nucleolus | 0.000025 | 0.000142 | 0.000112 | 0.000163 | 0.000149 | 0.000132 | 0.000081 | 0.000056 | 0.000135 | 0.000136 | 0.000085 | 0.000162 |
Nucleus | 0.000664 | 0.000771 | 0.000483 | 0.001634 | 0.000823 | 0.000247 | 0.000148 | 0.000561 | 0.000805 | 0.000447 | 0.000187 | 0.001615 |
Peroxisomes | 0.000291 | 0.000058 | 0.000008 | 0.000101 | 0.000011 | 0.000127 | 0.000010 | 0.000001 | 0.000043 | 0.000100 | 0.000009 | 0.000100 |
Vacuole | 0.183158 | 0.060027 | 0.186275 | 0.024318 | 0.033372 | 0.052731 | 0.013409 | 0.010423 | 0.050403 | 0.055510 | 0.033238 | 0.024073 |
Vacuole Periphery | 0.006780 | 0.006612 | 0.008020 | 0.004588 | 0.007338 | 0.006928 | 0.002792 | 0.000919 | 0.007275 | 0.006808 | 0.003391 | 0.004523 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.08 | 2.17 | -1.50 | -3.27 | -4.45 | -2.91 | -0.86 | -1.15 | -0.93 | -1.09 | -3.09 | 0.41 | -4.57 | -3.86 | -4.62 |
Bud Neck | 1.59 | 1.15 | 1.66 | 0.38 | 0.97 | -1.76 | 0.61 | -1.27 | -0.94 | -1.38 | -1.12 | 1.27 | -2.66 | -2.18 | -4.15 |
Bud Site | 0.48 | 2.40 | -0.75 | -3.24 | -9.93 | -1.50 | 2.02 | -1.24 | -1.18 | -1.30 | -2.90 | 3.12 | -7.84 | -6.53 | -9.90 |
Cell Periphery | -4.81 | -2.10 | -5.54 | -2.68 | -6.09 | 9.23 | -6.61 | 0.29 | -1.00 | 1.12 | 5.47 | -5.42 | 4.47 | -0.01 | 10.80 |
Cytoplasm | 0.57 | 3.51 | -0.40 | -2.12 | -12.22 | 1.86 | 3.09 | 3.03 | 3.48 | -0.62 | -1.95 | 4.58 | -8.59 | -7.66 | -12.20 |
Cytoplasmic Foci | 1.81 | 1.96 | 1.62 | -1.77 | -3.59 | -1.09 | -1.02 | 1.91 | 1.67 | 1.15 | 0.84 | -0.39 | -0.48 | -1.67 | 0.20 |
Eisosomes | -4.94 | -4.69 | 0.52 | 6.21 | 4.91 | 0.81 | -2.05 | -1.78 | -2.31 | -0.50 | -1.00 | -3.15 | 2.51 | 6.80 | 6.19 |
Endoplasmic Reticulum | 0.30 | 0.67 | 2.22 | 3.45 | 1.92 | -10.97 | 0.53 | 0.41 | 10.98 | -0.09 | -10.98 | 0.82 | -0.94 | 10.94 | -1.99 |
Endosome | 2.07 | 2.14 | 2.03 | -0.62 | -1.94 | -5.87 | -0.65 | 1.07 | 7.08 | 0.90 | 0.74 | 1.50 | 0.82 | 0.30 | -1.14 |
Golgi | 1.67 | 1.70 | 1.78 | 1.76 | 1.63 | -3.93 | 2.15 | 2.54 | 8.08 | 1.19 | -0.14 | 2.34 | 1.23 | 3.60 | -3.76 |
Lipid Particles | -0.84 | 1.67 | 0.98 | 2.31 | -1.73 | -2.92 | 3.28 | 3.23 | 3.51 | -0.06 | -4.15 | 2.84 | -3.47 | 1.25 | -4.99 |
Mitochondria | 0.47 | 1.02 | -1.10 | -2.93 | -3.97 | -4.37 | -1.24 | -0.76 | 0.39 | 0.67 | -2.39 | -1.00 | -3.71 | -3.14 | -2.05 |
Mitotic Spindle | -3.58 | -4.71 | -1.61 | -0.51 | 0.61 | -19.70* | 3.01 | 0.47 | 13.58 | -1.03 | -19.03 | -0.59 | -2.11 | 0.02 | -2.05 |
None | -2.83 | -0.42 | -4.59 | 1.47 | -5.31 | 0.19 | 2.88 | 2.53 | 3.13 | -0.27 | -2.64 | 2.69 | -5.06 | -0.38 | -6.62 |
Nuclear Periphery | -7.25 | -5.90 | -2.21 | 6.86 | 5.70 | -18.15* | 3.62 | 3.07 | 18.41 | 1.43 | -19.34 | -4.23 | -9.80 | 18.43 | 1.24 |
Nuclear Periphery Foci | 0.03 | 3.63 | 3.09 | 3.25 | -0.25 | -4.68 | 1.89 | 1.62 | 12.50 | -0.45 | -4.77 | 2.95 | -0.78 | 2.69 | -2.68 |
Nucleolus | -15.37* | -7.22 | -1.72 | -0.36 | -0.70 | 0.66 | 1.96 | 2.61 | 5.10 | 1.48 | 0.14 | 1.66 | -0.34 | -0.42 | -1.00 |
Nucleus | -0.48 | 0.78 | -3.74 | -4.56 | -5.38 | 1.22 | 1.39 | 0.51 | -0.74 | -0.96 | 0.89 | 1.44 | -1.51 | -7.08 | -8.71 |
Peroxisomes | 1.55 | 1.89 | 1.26 | -2.46 | -5.78 | -9.43 | 0.13 | 6.13 | 10.30 | 1.46 | -2.97 | 1.82 | -2.43 | -0.01 | -5.40 |
Vacuole | 5.11 | -0.10 | 6.64 | 11.11 | 10.73 | -6.19 | 7.33 | 5.14 | 10.11 | 0.77 | -1.25 | 3.72 | 6.49 | 14.79 | 3.03 |
Vacuole Periphery | 0.05 | -0.31 | 0.60 | 3.73 | 2.43 | 0.50 | 5.25 | 7.51 | 20.57 | 5.47 | 0.56 | 4.38 | 3.09 | 6.39 | -2.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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