Standard name
Human Ortholog
Description Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0.09 0.07 0.12 0.21 0.41 0.48 0.43 0.45 0.5 0.51 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.91 0.88 0.96 0.89 0.84 0.83 0.81 0.71 0.83 0.76 0.78 0.9 0.86 0.89 0.84 0.88 0.88 0.84 0.85 0.93 0.79 0.88 0.86
Cytoplasm 0.1 0.13 0 0.06 0.08 0.1 0.12 0.09 0.1 0.14 0.1 0 0 0.05 0.21 0.14 0.13 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.08 0 0 0.09 0.06 0.07 0.09 0.08 0.1 0.07 0 0 0.07 0.11 0 0 0.11 0.06 0.05
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0.06 0.11 0.14 0.14 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 20 15 20 38 86 98 108 84 114 89 14 4 5 24 6 4 0 2 1 5 5 5
Bud 2 8 2 3 5 6 11 13 9 11 5 0 0 1 9 9 3 0 3 3 5 1 8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 2
Bud Site 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 75 199 215 152 154 174 165 180 156 175 137 458 300 286 339 289 254 87 289 262 287 323 387
Cytoplasm 8 29 7 10 15 21 24 22 18 32 18 15 13 15 83 45 38 3 18 2 8 4 6
Endoplasmic Reticulum 3 8 2 7 15 4 8 22 11 17 15 39 35 21 13 6 20 11 12 0 40 20 21
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 0 2 0 0 0 2 0 0 1 1 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 2 13 7 14 21 32 24 0 0 0 21 13 14 1 8 7 8 5 11
Nucleus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 0 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 0 1 0 0 1 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 3 1 4 1 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 1 0 0 0 2 0 0 0 1 0 3 0 0 2 0 1 0 0
Unique Cell Count 82 225 225 170 184 210 203 253 187 230 176 511 348 320 402 330 288 105 341 281 363 366 449
Labelled Cell Count 96 264 243 193 236 307 317 372 299 384 288 530 357 331 490 373 336 105 341 281 363 366 449


Bud, Cell Periphery, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.1 58.3 55.3 47.7 35.2 31.0 30.4 31.9 32.4 31.2 31.7 75.7 79.7 81.7 41.6 52.6 59.9 39.8 41.5 46.6
Std Deviation (1e-4) 9.7 15.1 12.7 12.0 12.5 11.6 11.9 10.6 11.2 11.2 10.3 18.2 20.2 19.5 14.0 16.3 19.8 11.2 12.5 13.3
Intensity Change (Log2) -0.21 -0.65 -0.84 -0.86 -0.79 -0.77 -0.83 -0.8 0.45 0.53 0.56 -0.41 -0.07 0.11 -0.48 -0.41 -0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 4.2 8.5 9.8 9.0 9.0 10.2 10.0 -2.5 -3.6 -3.1 -0.3 -2.9 -3.1
Bud 0 0 0 2.7 2.7 0 2.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -4.0 -4.3 -4.7 -7.0 -4.1 -5.9 -5.4 -2.6 -3.6 -2.6 -4.2 -3.2 -3.0
Cytoplasm 1.3 2.2 2.9 3.5 2.6 2.8 4.1 2.9 -0.1 0.4 0.9 6.0 4.2 4.0
Endoplasmic Reticulum 0 3.7 0 0 3.9 2.9 3.5 3.8 3.7 4.4 3.2 1.8 0 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 0 3.6 5.2 5.8 5.7 0 0 0 3.5 3.0 3.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 58.6619 86.2924 79.048 69.4903 68.8493 76.483 46.2354 63.8785 56.0166 54.0674 54.561 58.0218 91.1106 109.6637 95.8975 95.2188 91.9875 99.3925
Actin 0.0155 0.0001 0.0005 0.0032 0.0046 0.0063 0.0426 0.0004 0.0139 0.0214 0.0749 0.0021 0.0006 0 0.0001 0.0001 0.0001 0.002
Bud 0.0014 0.0001 0.0003 0.0004 0.0009 0.0005 0.0011 0.0001 0.0003 0.0003 0.0001 0.0003 0.0003 0 0.0001 0.0001 0.0001 0.0003
Bud Neck 0.0041 0.0003 0.0003 0.0002 0.0024 0.0017 0.0045 0.0003 0.0005 0.0052 0.0007 0.0033 0.0011 0.0002 0.0013 0.0012 0.0026 0.0106
Bud Periphery 0.0036 0.0003 0.0008 0.0007 0.0022 0.0021 0.0054 0.0003 0.0013 0.0007 0.0006 0.0021 0.0016 0.0002 0.0006 0.0011 0.0009 0.0039
Bud Site 0.0075 0.0005 0.0006 0.0008 0.0022 0.0004 0.0081 0.0017 0.0109 0.0084 0.0003 0.0005 0.0053 0.0004 0.0002 0.0001 0.0001 0.0002
Cell Periphery 0.3769 0.5313 0.4457 0.373 0.4074 0.4162 0.25 0.354 0.2895 0.2393 0.2475 0.2277 0.4736 0.6017 0.5453 0.5563 0.4536 0.4674
Cytoplasm 0.0628 0.0809 0.068 0.0154 0.0765 0.062 0.0135 0.0212 0.0162 0.0096 0.0014 0.0207 0.0085 0.0158 0.0161 0.0043 0.0732 0.0593
Cytoplasmic Foci 0.0253 0.0008 0.0038 0.0403 0.0115 0.0028 0.0129 0 0.0031 0.0037 0.0048 0.0023 0.0045 0 0 0 0 0.0001
Eisosomes 0.0028 0.0001 0.0003 0.0004 0.0019 0.0039 0.0028 0.0004 0.0012 0.0003 0.0003 0.0005 0.0012 0.0005 0.0004 0.0011 0.0001 0.0005
Endoplasmic Reticulum 0.2609 0.2414 0.3266 0.3444 0.2619 0.3163 0.5244 0.5439 0.5852 0.6269 0.584 0.6621 0.3038 0.1787 0.2321 0.1702 0.2992 0.2703
Endosome 0.0143 0.0001 0.0008 0.0052 0.0089 0.0114 0.0109 0 0.0006 0.0018 0.0016 0.0006 0.0013 0 0 0 0 0.0001
Golgi 0.0036 0 0.0002 0.0027 0.0019 0.0039 0.0045 0 0.0012 0.0032 0.0038 0.0004 0.0002 0 0 0.0001 0 0.0001
Lipid Particles 0.0237 0.0143 0.011 0.0313 0.0124 0.0149 0.0106 0.0053 0.0048 0.0078 0.0051 0.0044 0.0065 0.0054 0.0047 0.0034 0.0042 0.0084
Mitochondria 0.0128 0.0001 0.014 0.0137 0.0402 0.0144 0.007 0.0002 0.0012 0.0008 0.0002 0.0024 0.0003 0 0 0 0 0
None 0.0247 0.0016 0.0016 0.0091 0.0194 0.0014 0.0023 0.0001 0.0049 0.0001 0.0003 0.0027 0.0049 0 0 0 0 0
Nuclear Periphery 0.0502 0.0401 0.059 0.0468 0.039 0.0374 0.0421 0.042 0.0359 0.0397 0.0496 0.032 0.1012 0.1076 0.1197 0.1532 0.0692 0.082
Nucleolus 0.0057 0.0003 0.0014 0.0025 0.0039 0.0005 0.0038 0.0005 0.0016 0.0001 0 0.0008 0.0005 0 0.0001 0.0001 0 0.0002
Nucleus 0.0071 0.0006 0.0011 0.002 0.0017 0.0016 0.0115 0.0028 0.0011 0.0003 0.0002 0.0012 0.001 0.0003 0.0005 0.0008 0.0008 0.0017
Peroxisomes 0.0027 0 0.0012 0.0034 0.0019 0.0052 0.0071 0 0.0003 0.0069 0.0073 0.0002 0.0001 0 0 0 0 0
Punctate Nuclear 0.0073 0 0.0002 0.0132 0.0009 0.0014 0.0041 0 0.0018 0.0002 0.0018 0.0107 0.0011 0 0 0 0 0.0001
Vacuole 0.0823 0.0872 0.0617 0.0838 0.0967 0.094 0.0294 0.0265 0.0243 0.023 0.0154 0.0221 0.0818 0.0889 0.0784 0.1068 0.0949 0.0918
Vacuole Periphery 0.0047 0.0001 0.0009 0.0076 0.0014 0.0016 0.0015 0.0001 0.0003 0.0002 0.0002 0.0006 0.0007 0.0002 0.0005 0.0011 0.0008 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 193.1391 485.7038 711.2511 634.2111 214.8538 362.9813 740.5561 773.8664 758.72 742.0451
Translational Efficiency 0.9441 0.9909 1.0856 0.9534 1.0633 0.7793 0.9315 1.025 0.9921 0.9191

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cell periphery (21%), cytoplasm (6%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fet3

Fet3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fet3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available