Standard name
Human Ortholog
Description Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.05 – 0 0 0 0 0.09 0.14 0.18 0.15 – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.17 0 0 0 0 0 0.06 – 0.23 0.11 0.39 0.16 0 0 0 0.05 – – 0 0 0 0 0 0
Nucleus 0.94 0.89 0.92 0.92 0.91 0.84 0.84 – 0.78 0.85 0.75 0.77 0.89 0.81 0.77 0.65 – – 0.8 0.83 0.76 0.85 0.77 0.77
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0.29 0.14 0.05 0 0 0.13 0.1 – 0.15 0.11 0.22 0.21 0 0.09 0.07 0.17 – – 0.14 0.11 0.1 0.07 0.09 0.08
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 – – 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 1 0 1 0
Bud 0 0 0 0 0 0 1 – 3 1 6 1 0 0 0 0 – – 0 2 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 2 0 1 2 – 4 4 10 7 0 0 0 1 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0 7 5 5 8 15 17 – 8 9 5 10 16 34 52 6 – – 0 0 1 2 6 3
Endoplasmic Reticulum 0 1 1 0 0 0 2 – 0 0 0 0 0 0 1 1 – – 0 0 0 0 0 0
Endosome 0 1 3 1 2 4 4 – 0 1 0 2 2 2 2 0 – – 1 2 2 1 3 7
Golgi 0 0 3 0 2 0 0 – 0 0 0 0 0 0 2 1 – – 1 2 1 0 0 0
Mitochondria 18 7 0 1 3 10 21 – 53 39 128 48 0 2 0 2 – – 0 2 1 1 2 1
Nucleus 100 334 148 134 208 228 301 – 182 300 249 229 159 198 229 26 – – 87 346 133 139 196 212
Nuclear Periphery 0 0 0 1 2 3 1 – 1 5 2 3 0 1 1 0 – – 0 1 0 0 0 0
Nucleolus 31 51 8 6 9 36 36 – 36 38 72 63 5 21 21 7 – – 15 45 18 11 23 21
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 0 – – 0 0 0 0 0 0
SpindlePole 0 1 7 1 4 3 6 – 4 10 3 9 1 2 5 1 – – 0 3 2 0 5 7
Vac/Vac Membrane 1 10 4 1 3 1 7 – 2 1 0 4 2 1 1 2 – – 1 7 8 3 10 11
Unique Cell Count 106 374 161 145 229 273 358 233 352 330 296 178 244 297 40 110 418 175 164 257 277
Labelled Cell Count 150 412 179 152 241 301 398 293 408 476 376 185 261 317 47 110 418 175 164 257 277


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.9 7.7 7.6 7.6 6.3 6.7 – 5.7 5.6 4.7 5.4 8.1 8.2 7.9 9.2 – – 7.1 7.7 7.9
Std Deviation (1e-4) 0.8 1.2 1.6 2.2 1.9 1.7 2.7 – 2.1 1.1 0.8 1.4 1.7 1.9 1.9 2.9 – – 1.0 1.7 1.5
Intensity Change (Log2) – – – -0.01 -0.02 -0.28 -0.2 – -0.43 -0.45 -0.7 -0.52 0.08 0.09 0.04 0.26 – – -0.11 0.0 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Cytoplasm 0 0.2 1.1 0.9 – 0.2 0 0 0.2 2.2 3.6 4.5 0 – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Mitochondria 0 0 0 3.1 – 6.5 4.4 0 5.4 0 0 0 0 – –
Nucleus 0.2 -0.4 -2.5 -2.4 – -3.7 -2.1 -4.4 -3.9 -0.8 -3.0 -4.0 -4.5 – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
Nucleolus -0.3 -0.5 2.7 1.9 – 3.2 2.1 0 4.6 -1.0 1.4 0.9 0 – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 – 0 -0.9 0 -0.7 0 0 0 0 – –
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5593 2.439 2.3468 1.6755 1.2427 2.2926 2.0557 3.0875 2.2522 1.9953 1.7301 2.1161 3.3713 3.5975 3.135 3.3956 3.2437 3.1672
Actin 0.0159 0.0001 0.0055 0.0167 0 0.0005 0.0142 0 0.0003 0.0629 0 0 0.0321 0.0001 0.005 0.0535 0.0005 0
Bud 0.0004 0 0.0004 0.0004 0.0001 0.0001 0.0007 0 0.0001 0.0008 0 0 0.0005 0 0.0004 0.0002 0.0002 0
Bud Neck 0.0003 0 0.0002 0.0001 0.0005 0.0008 0.0008 0.0001 0.0005 0.0049 0.0002 0.0006 0.0015 0 0.0006 0.0029 0.0006 0.0005
Bud Periphery 0.0004 0.0001 0.0006 0.0007 0.0002 0.0001 0.0012 0 0.0001 0.0013 0.0001 0 0.0011 0 0.0005 0.0005 0.0003 0
Bud Site 0.0015 0 0.001 0.001 0.0003 0.0001 0.0022 0 0.0003 0.0033 0.0001 0 0.0017 0 0.0028 0.0007 0.0004 0
Cell Periphery 0.0002 0 0.0001 0.0001 0.0001 0 0.0003 0 0 0.0003 0 0 0.0004 0 0.0002 0.0001 0 0
Cytoplasm 0.0216 0.0002 0.0035 0.0005 0 0.0126 0.0012 0 0.0005 0.0019 0 0 0.0054 0.0014 0.0015 0.0031 0.0003 0.0002
Cytoplasmic Foci 0.0302 0 0.0162 0.009 0 0.0123 0.0052 0 0.0003 0.0603 0 0 0.0151 0.0007 0.0046 0.0025 0.0107 0.0002
Eisosomes 0 0 0.0001 0.0001 0 0 0.0001 0 0 0.0004 0 0 0.0003 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0031 0 0.0013 0.0021 0 0.0012 0.0014 0 0.0002 0.006 0 0 0.0053 0.0003 0.0003 0.0021 0.0001 0.0001
Endosome 0.0227 0.0001 0.0201 0.0224 0.0001 0.002 0.0155 0 0.0004 0.0425 0 0 0.0198 0.0009 0.0023 0.0145 0.0094 0.0004
Golgi 0.0082 0 0.0082 0.0237 0 0.0009 0.0079 0 0.0001 0.0318 0 0 0.0062 0 0.0014 0.0051 0.0026 0
Lipid Particles 0.0131 0 0.0155 0.0249 0.0001 0.0044 0.0094 0 0.0001 0.0439 0 0 0.0184 0.0002 0.0079 0.0026 0.0111 0.0001
Mitochondria 0.005 0.0113 0.0354 0.0807 0.0008 0.0002 0.0033 0.0001 0.0003 0.0127 0.0004 0.0001 0.0056 0.0001 0.004 0.0029 0.0021 0.0001
None 0.0014 0.0001 0.0032 0.0005 0.0001 0.0003 0.0006 0 0.0001 0.001 0 0 0.0071 0.0037 0.0005 0.002 0.0005 0.0001
Nuclear Periphery 0.0141 0.0012 0.0042 0.0148 0.0005 0.0048 0.0081 0.0007 0.0033 0.0173 0.0006 0.0001 0.0249 0.0031 0.0017 0.0092 0.0032 0.0017
Nucleolus 0.0513 0.0188 0.0337 0.0084 0.3841 0.0982 0.0274 0.0297 0.0191 0.0434 0.1622 0.0776 0.0323 0.0248 0.0299 0.0085 0.1893 0.086
Nucleus 0.7387 0.9619 0.8313 0.7814 0.6053 0.834 0.8903 0.9689 0.9687 0.5911 0.8341 0.9206 0.7786 0.9594 0.916 0.8825 0.7497 0.9083
Peroxisomes 0.0148 0 0.0128 0.0085 0 0.0037 0.0024 0 0.0001 0.0624 0 0 0.0074 0 0.0038 0.001 0.0095 0
Punctate Nuclear 0.0511 0.005 0.003 0.0019 0.007 0.0234 0.0028 0.0004 0.0052 0.0078 0.0022 0.0009 0.0293 0.0048 0.0137 0.0047 0.0087 0.0022
Vacuole 0.0037 0.0003 0.0019 0.0006 0.0003 0.0003 0.0039 0 0.0001 0.0021 0.0001 0 0.0044 0.0002 0.0023 0.0005 0.0004 0.0001
Vacuole Periphery 0.0022 0.0006 0.0017 0.0014 0.0002 0.0001 0.0012 0 0.0001 0.0024 0 0 0.0025 0.0001 0.0005 0.0006 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.3266 28.1268 11.875 27.1953 23.2105 21.145 20.7163 17.8706 19.3476 29.014
Translational Efficiency 1.0598 0.7824 1.721 0.7221 0.9492 1.0787 0.8434 0.9984 1.0655 0.8905

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1004 1091 268 449 2014 977 1763 169 3018 2068 2031 618

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 768.97 766.94 1053.84 1034.41 817.25 857.77 971.46 1096.66 801.19 809.85 982.33 1051.43
Standard Deviation 100.53 130.81 111.84 175.32 113.43 127.52 131.97 144.86 111.65 136.99 132.46 169.82
Intensity Change Log 2 — -0.003814 0.454657 0.427809 — 0.069813 0.249377 0.424267 — 0.034589 0.352543 0.425985

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000878 0.001308 0.001343 0.002131 0.000391 0.000978 0.000538 0.017505 0.000553 0.001153 0.000644 0.006335
Bud Neck 0.005076 0.007356 0.016441 0.038447 0.005856 0.007777 0.024587 0.034283 0.005596 0.007555 0.023512 0.037309
Bud Site 0.002087 0.004235 0.001678 0.022779 0.000724 0.002582 0.000970 0.011617 0.001177 0.003454 0.001063 0.019726
Cell Periphery 0.000128 0.000196 0.000037 0.000296 0.000064 0.000065 0.000030 0.000197 0.000085 0.000134 0.000031 0.000269
Cytoplasm 0.038434 0.021535 0.000126 0.009313 0.006495 0.002764 0.000437 0.002932 0.017120 0.012667 0.000396 0.007568
Cytoplasmic Foci 0.003316 0.003122 0.000028 0.004783 0.000531 0.003652 0.000010 0.009462 0.001457 0.003372 0.000012 0.006062
Eisosomes 0.000029 0.000041 0.000060 0.000047 0.000023 0.000032 0.000031 0.000060 0.000025 0.000037 0.000034 0.000051
Endoplasmic Reticulum 0.002494 0.003974 0.002846 0.004327 0.001895 0.002486 0.001945 0.003212 0.002094 0.003271 0.002064 0.004022
Endosome 0.001397 0.001725 0.000101 0.010458 0.000272 0.001921 0.000094 0.014216 0.000646 0.001818 0.000095 0.011486
Golgi 0.001157 0.002008 0.000201 0.005087 0.000424 0.002044 0.000116 0.018378 0.000668 0.002025 0.000127 0.008722
Lipid Particles 0.000736 0.000917 0.000071 0.000350 0.000111 0.000457 0.000019 0.000401 0.000319 0.000699 0.000026 0.000364
Mitochondria 0.002040 0.002927 0.001256 0.010845 0.001296 0.006696 0.000980 0.038468 0.001544 0.004708 0.001017 0.018399
Mitotic Spindle 0.005202 0.002611 0.001377 0.052048 0.001818 0.008392 0.001923 0.049752 0.002944 0.005342 0.001851 0.051420
None 0.032265 0.030626 0.001355 0.005822 0.007368 0.004129 0.001665 0.004196 0.015650 0.018108 0.001624 0.005378
Nuclear Periphery 0.000286 0.000202 0.000125 0.002434 0.000157 0.000410 0.000280 0.000706 0.000200 0.000300 0.000260 0.001962
Nuclear Periphery Foci 0.002309 0.002803 0.000075 0.001945 0.000735 0.000848 0.000087 0.000740 0.001259 0.001879 0.000086 0.001615
Nucleolus 0.098515 0.118232 0.109820 0.032626 0.100159 0.124117 0.067878 0.021167 0.099612 0.121012 0.073412 0.029492
Nucleus 0.799725* 0.789687* 0.860354* 0.767264* 0.870553* 0.825684* 0.897773* 0.743681* 0.846991* 0.806693* 0.892836* 0.760815*
Peroxisomes 0.001241 0.001953 0.001865 0.001020 0.000383 0.001212 0.000256 0.001127 0.000668 0.001603 0.000469 0.001050
Vacuole 0.001875 0.003461 0.000648 0.018655 0.000487 0.001667 0.000234 0.014391 0.000949 0.002613 0.000289 0.017489
Vacuole Periphery 0.000810 0.001081 0.000197 0.009322 0.000258 0.002087 0.000146 0.013510 0.000442 0.001556 0.000153 0.010467

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.23 -5.23 -6.21 -4.29 -3.45 -3.97 -7.78 -1.52 -1.40 -1.47 -6.52 -4.63 -2.41 -1.97 -2.29
Bud Neck -2.71 -11.73 -11.51 -10.84 -6.38 -2.90 -19.66 -5.54 -5.22 -1.80 -3.75 -21.57 -12.48 -11.78 -4.33
Bud Site -2.38 0.19 -4.01 -3.42 -4.04 -2.13 -4.07 -3.89 -3.02 -3.70 -3.88 -0.40 -5.26 -4.30 -5.24
Cell Periphery -3.11 6.64 -2.46 -0.55 -5.04 -0.15 3.74 -2.89 -2.71 -4.13 -3.75 7.38 -4.23 -2.28 -6.37
Cytoplasm 3.77 10.50 8.01 4.90 -3.39 2.44 5.35 2.24 -0.13 -2.68 2.08 11.51 6.05 3.82 -3.74
Cytoplasmic Foci 0.63 4.25 1.92 1.43 -2.65 -2.86 2.82 -1.58 0.06 -1.92 -2.33 5.02 -0.67 1.23 -3.25
Eisosomes -3.16 -8.39 -6.77 -3.69 1.70 -5.29 -8.14 -6.16 -4.67 -4.83 -6.61 -8.65 -10.86 -6.52 -7.48
Endoplasmic Reticulum -5.40 -2.00 -5.71 -3.00 -4.55 -3.03 -1.89 -5.94 -4.68 -5.58 -7.20 -1.22 -8.32 -5.28 -8.06
Endosome 0.34 3.68 -1.89 -2.15 -3.69 -2.66 3.18 -2.31 -1.54 -2.39 -2.61 4.29 -3.64 -2.60 -4.41
Golgi -2.06 4.14 -0.88 0.02 -1.98 -3.14 2.17 -1.68 -1.26 -1.76 -4.33 4.62 -2.16 -1.05 -2.61
Lipid Particles -0.38 4.55 2.50 1.36 -5.87 -2.24 3.79 -3.27 0.35 -4.46 -1.61 5.67 -0.72 1.42 -8.25
Mitochondria -1.58 1.34 -3.32 -2.70 -3.82 -3.31 0.23 -2.19 -1.41 -2.21 -3.78 0.93 -3.66 -2.16 -3.85
Mitotic Spindle 1.77 0.96 -5.08 -5.74 -5.46 -3.16 -0.87 -3.14 -2.53 -3.05 -1.63 0.28 -6.37 -5.99 -6.42
None 0.58 11.92 10.18 8.25 -4.67 2.90 6.59 3.69 0.42 -3.24 -1.12 13.24 9.26 7.64 -5.29
Nuclear Periphery 2.84 3.61 -7.27 -7.69 -7.77 -2.48 -4.54 -5.34 -4.00 -4.38 -1.75 -3.41 -8.82 -8.24 -8.07
Nuclear Periphery Foci -0.46 7.40 3.72 2.88 -4.63 -0.96 3.65 0.33 1.14 -2.64 -1.86 7.82 1.51 2.73 -5.38
Nucleolus -2.40 -1.91 11.93 16.13 9.69 -4.55 6.91 13.87 15.63 8.33 -5.12 6.13 18.33 21.88 11.68
Nucleus -0.16 -4.71 -1.45 -1.38 2.20 6.07 -3.36 2.98 0.91 3.73 6.26 -8.16 3.14 -0.08 6.59
Peroxisomes -1.65 -1.90 1.64 3.48 5.50 -3.82 1.83 -3.99 0.63 -4.79 -4.22 1.85 -1.75 3.46 -5.31
Vacuole -1.39 3.91 -4.24 -3.70 -4.85 -2.93 1.40 -3.69 -3.28 -3.75 -3.31 4.72 -5.70 -4.89 -6.06
Vacuole Periphery -1.13 3.16 -2.36 -2.15 -2.83 -2.70 1.78 -1.72 -1.07 -1.76 -3.31 3.79 -3.10 -2.13 -3.34
Standard name
Human Ortholog
Description Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rna14

Rna14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rna14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available