Standard name
Human Ortholog
Description Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.09 0.05 0.07 – 0.05 0 0 0 0.13 0.2 0.25 0.17 0.15 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 0 0.06 0 0.12 0.15 – 0.27 0.18 0.42 0.23 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.92 0.93 0.9 0.89 0.86 0.88 0.84 – 0.8 0.84 0.75 0.8 0.85 0.78 0.73 0.69 0.69 0.65 0.78 0.76 0.71 0.74 0.66 0.62
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 – 0.08 0.08 0.16 0.17 0 0 0 0 0 0 0.1 0.1 0.11 0.12 0.16 0.13
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.14 0.19 0 0.07 0.08 0.07 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2
Bud 1 0 0 0 0 2 4 – 1 5 2 1 0 0 0 1 0 0 2 3 2 1 2 5
Bud Neck 0 0 0 0 3 0 2 – 0 0 0 3 0 0 0 0 0 0 0 0 2 1 1 2
Bud Site 0 0 2 2 0 4 5 – 7 12 18 24 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 3 17 18 15 28 18 27 – 17 16 18 5 50 100 124 69 73 44 0 3 2 2 7 12
Endoplasmic Reticulum 2 2 2 2 0 0 0 – 0 0 0 0 0 1 1 18 23 22 0 0 0 1 0 0
Endosome 2 6 7 0 3 2 0 – 0 1 0 0 2 1 4 9 17 15 4 6 6 1 5 7
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 3 2 1 0 1 1
Mitochondria 65 8 1 15 7 44 62 – 99 87 172 102 1 3 1 22 10 13 5 4 2 2 4 12
Nucleus 303 430 285 226 281 314 345 – 290 410 306 352 317 385 367 285 329 226 262 416 244 169 294 326
Nuclear Periphery 0 0 0 0 0 2 2 – 2 2 1 5 0 1 0 0 0 1 0 1 1 0 0 2
Nucleolus 11 14 3 3 7 18 16 – 30 41 63 76 2 4 2 0 0 5 34 56 38 27 70 67
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 3 8 24 8 12 4 13 – 7 18 3 13 12 16 17 5 6 4 6 9 7 7 15 23
Vac/Vac Membrane 1 11 2 2 3 2 3 – 2 3 1 5 0 2 2 51 68 65 14 35 25 15 33 52
Unique Cell Count 329 464 316 254 325 356 412 362 489 406 439 371 492 500 416 476 348 337 545 342 231 443 530
Labelled Cell Count 391 497 344 273 344 410 479 455 595 585 586 384 514 519 461 528 396 337 545 342 231 443 530


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.8 6.5 5.9 6.5 5.4 5.4 – 4.9 5.1 4.7 4.9 6.3 6.4 6.0 6.5 6.5 6.4 5.7 6.1 6.1
Std Deviation (1e-4) 0.6 0.9 2.1 1.5 1.8 1.6 1.6 – 1.3 1.2 1.5 1.1 1.2 1.6 1.4 1.3 1.3 1.4 1.1 1.3 1.5
Intensity Change (Log2) – – – -0.15 0.01 -0.28 -0.26 – -0.41 -0.34 -0.47 -0.39 -0.04 -0.02 -0.11 0.0 0.0 -0.02 -0.2 -0.08 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 1.9 0 3.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 1.4 -0.4 0.5 – -0.6 -1.7 -0.8 -3.6 3.4 5.7 7.0 4.5 4.2 3.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.0 3.3 3.9
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 1.1 1.5
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.0 0 6.2 7.0 – 0 7.8 0 9.1 0 0 0 3.8 0 3.1
Nucleus -0.5 -1.5 -0.8 -2.5 – -3.6 -2.6 -5.1 -3.7 -1.9 -4.4 -5.8 -7.0 -7.0 -7.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 2.5 – 0 4.5 0 7.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.3 -2.1 -4.2 -2.7 – 0 -2.4 0 -2.9 -2.6 -2.8 -2.7 -4.4 -4.6 -4.1
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 6.0 6.6 7.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0171 0 0.0425 0.0003 0.0004 0 0.0351 0.0001 0.0164 0.0327 0.0022 0.0098 – – – – – –
Bud 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0006 0.0004 0.0001 0.0017 0.0003 0.0004 – – – – – –
Bud Neck 0.0005 0.0002 0.0002 0.0001 0.0009 0.0015 0.0073 0.0002 0.0012 0.0021 0.0005 0.0013 – – – – – –
Bud Periphery 0.0006 0 0.0001 0.0006 0.0003 0.0001 0.0021 0.0016 0.0003 0.0065 0.0004 0.0013 – – – – – –
Bud Site 0.0016 0.0002 0.0006 0.0001 0.0002 0.0002 0.01 0.0007 0.0036 0.0023 0.0009 0.0005 – – – – – –
Cell Periphery 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0014 0.0003 0.0008 0.0005 0.0001 0.0001 – – – – – –
Cytoplasm 0.002 0.0008 0.0005 0 0.0041 0.0001 0.0078 0.0014 0.0006 0.0006 0.0024 0.0005 – – – – – –
Cytoplasmic Foci 0.0236 0 0.0002 0 0.0004 0 0.0069 0 0.0003 0.0034 0.006 0.0096 – – – – – –
Eisosomes 0.0008 0 0.0001 0.0001 0.0001 0 0.0005 0 0.0004 0.0004 0.0001 0.0001 – – – – – –
Endoplasmic Reticulum 0.0018 0.0001 0.0001 0.0003 0.0002 0 0.0031 0.0001 0.0002 0.0007 0.0006 0.0004 – – – – – –
Endosome 0.0231 0 0.0002 0.0005 0.001 0 0.0104 0.0001 0.0001 0.0047 0.025 0.0024 – – – – – –
Golgi 0.01 0 0.0021 0.0002 0.0001 0 0.0087 0 0.0004 0.0096 0.0066 0.002 – – – – – –
Lipid Particles 0.1671 0 0.0001 0.0001 0.0003 0 0.0079 0 0.0002 0.0048 0.0055 0.0065 – – – – – –
Mitochondria 0.0089 0.0003 0.0004 0.1056 0.0007 0.0001 0.0359 0.0087 0.0391 0.0499 0.0668 0.0464 – – – – – –
None 0.0059 0.0013 0.0007 0.0001 0.0202 0 0.005 0.0017 0.0013 0.0008 0.0151 0.0003 – – – – – –
Nuclear Periphery 0.0059 0.0031 0.0022 0.0008 0.0235 0.0005 0.0154 0.0044 0.0007 0.0029 0.0109 0.001 – – – – – –
Nucleolus 0.0347 0.0905 0.0267 0.0163 0.1051 0.0975 0.0255 0.0448 0.0242 0.0226 0.0445 0.0478 – – – – – –
Nucleus 0.63 0.9011 0.9205 0.8725 0.7981 0.8948 0.765 0.9203 0.9017 0.8338 0.7619 0.8398 – – – – – –
Peroxisomes 0.0413 0 0.0001 0 0.0001 0 0.0187 0 0.0054 0.0153 0.0066 0.0064 – – – – – –
Punctate Nuclear 0.0211 0.002 0.0019 0.0002 0.0436 0.0046 0.0277 0.0142 0.0023 0.0013 0.0405 0.0231 – – – – – –
Vacuole 0.0021 0.0001 0.0005 0.0006 0.0005 0.0002 0.0034 0.0004 0.0003 0.0022 0.002 0.0002 – – – – – –
Vacuole Periphery 0.0013 0.0001 0.0001 0.0014 0.0003 0 0.0016 0.0003 0.0004 0.0013 0.0011 0.0001 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7856 5.7599 9.6166 12.3733 12.879 13.9526 10.5556 11.6282 11.0283 12.9314
Translational Efficiency 1.4037 1.1447 0.9496 0.8667 0.7372 0.8023 0.7452 0.7282 1.0332 0.993

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1750 1533 1449 1280 372 1821 126 759 2122 3354 1575 2039

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 670.41 713.62 809.07 923.04 770.79 766.07 997.89 873.90 688.01 742.10 824.18 904.75
Standard Deviation 73.13 109.32 130.79 136.98 101.77 106.89 122.29 130.92 87.65 111.12 139.85 136.84
Intensity Change Log 2 — 0.090112 0.271221 0.461349 — -0.008862 0.372543 0.181130 — 0.038024 0.326294 0.318272

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000089 0.000576 0.000765 0.001757 0.000598 0.000651 0.002221 0.001107 0.000179 0.000617 0.000881 0.001515
Bud Neck 0.003774 0.008305 0.024216 0.056973 0.002280 0.011100 0.007807 0.044929 0.003512 0.009823 0.022904 0.052490
Bud Site 0.000730 0.002025 0.002668 0.025576 0.000751 0.002714 0.002500 0.016717 0.000733 0.002399 0.002654 0.022278
Cell Periphery 0.000094 0.000131 0.000095 0.000606 0.000072 0.000096 0.000086 0.000264 0.000090 0.000112 0.000094 0.000479
Cytoplasm 0.071742 0.025428 0.007621 0.016686 0.007992 0.010514 0.004760 0.039748 0.060567 0.017330 0.007392 0.025270
Cytoplasmic Foci 0.008535 0.002502 0.000112 0.004007 0.001753 0.005296 0.000276 0.004669 0.007346 0.004019 0.000125 0.004254
Eisosomes 0.000024 0.000028 0.000025 0.000042 0.000035 0.000039 0.000070 0.000046 0.000026 0.000034 0.000028 0.000043
Endoplasmic Reticulum 0.001237 0.003621 0.003484 0.007053 0.002671 0.004586 0.011168 0.006060 0.001488 0.004145 0.004099 0.006683
Endosome 0.000550 0.000908 0.000319 0.006703 0.000360 0.001913 0.000618 0.011207 0.000517 0.001454 0.000343 0.008379
Golgi 0.000182 0.000425 0.000126 0.009144 0.000375 0.001320 0.000197 0.002393 0.000215 0.000911 0.000131 0.006631
Lipid Particles 0.001841 0.001061 0.000109 0.000396 0.001063 0.001876 0.000739 0.000691 0.001704 0.001504 0.000159 0.000506
Mitochondria 0.002429 0.003961 0.001582 0.007954 0.000983 0.009214 0.001107 0.007073 0.002176 0.006813 0.001544 0.007626
Mitotic Spindle 0.000646 0.005726 0.003491 0.045140 0.002161 0.011463 0.021892 0.061296 0.000912 0.008841 0.004963 0.051154
None 0.020111 0.022815 0.008433 0.006946 0.008290 0.008703 0.004592 0.014739 0.018039 0.015153 0.008126 0.009847
Nuclear Periphery 0.001591 0.001096 0.000564 0.005040 0.000515 0.002324 0.000819 0.005707 0.001403 0.001763 0.000585 0.005288
Nuclear Periphery Foci 0.000882 0.001303 0.000246 0.001864 0.000863 0.001382 0.000661 0.001895 0.000879 0.001346 0.000279 0.001876
Nucleolus 0.028357 0.040785 0.023533 0.015596 0.047805 0.049844 0.014580 0.027546 0.031766 0.045704 0.022817 0.020044
Nucleus 0.855195* 0.875855* 0.920675* 0.761243* 0.919851* 0.869272* 0.911243* 0.724951* 0.866529* 0.872281* 0.919921* 0.747734*
Peroxisomes 0.000925 0.001125 0.000221 0.001082 0.000853 0.002624 0.000907 0.000857 0.000912 0.001939 0.000276 0.000998
Vacuole 0.000829 0.001567 0.001476 0.020369 0.000542 0.003543 0.013573 0.020633 0.000779 0.002640 0.002443 0.020467
Vacuole Periphery 0.000236 0.000756 0.000238 0.005822 0.000187 0.001525 0.000183 0.007472 0.000227 0.001174 0.000234 0.006436

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -14.67 -22.45 -17.33 -12.12 -9.30 -0.10 -7.72 -6.28 -10.17 4.18 -9.77 -17.09 -20.88 -15.14 -9.71
Bud Neck -6.86 -16.72 -21.31 -18.88 -9.26 -9.46 -7.73 -14.57 -11.23 -7.50 -10.79 -17.63 -25.56 -21.58 -9.85
Bud Site -3.87 -4.30 -7.89 -7.11 -6.27 -2.69 -5.02 -7.05 -6.13 -4.09 -5.14 -4.94 -10.49 -9.13 -7.96
Cell Periphery -2.64 0.63 -2.07 -1.70 -2.14 -0.91 -2.41 -3.86 -3.94 -1.90 -2.16 0.03 -2.67 -2.33 -2.67
Cytoplasm 11.23 17.98 15.85 5.27 -4.72 -1.02 0.88 -4.97 -5.36 -6.72 13.38 17.50 12.90 -0.42 -7.96
Cytoplasmic Foci 6.32 10.17 8.27 2.10 -3.25 -3.07 2.30 0.22 3.63 -2.93 4.32 10.39 8.13 4.38 -4.53
Eisosomes -0.89 -0.17 -9.52 -8.67 -9.90 -0.64 -6.44 -4.62 -5.79 4.18 -3.63 -2.39 -11.90 -8.98 -9.90
Endoplasmic Reticulum -10.85 -14.60 -20.31 -12.74 -12.73 -3.50 -9.23 -9.89 -8.30 4.19 -13.02 -14.79 -24.29 -14.39 -12.51
Endosome -1.92 2.20 -3.73 -3.09 -4.23 -4.81 -3.17 -3.73 -2.35 -3.05 -4.80 1.58 -5.19 -3.45 -5.54
Golgi -2.80 1.99 -2.92 -2.68 -2.99 -2.16 0.20 -1.40 0.54 -1.79 -3.43 1.59 -3.28 -2.08 -3.43
Lipid Particles 3.52 10.25 8.05 2.91 -8.65 -2.15 1.31 1.60 5.45 0.56 1.23 10.52 7.84 6.43 -10.68
Mitochondria -1.97 1.77 -5.47 -2.41 -7.92 -7.92 -3.99 -8.34 3.36 -6.86 -6.53 1.33 -7.27 1.51 -10.13
Mitotic Spindle -3.68 -4.00 -10.05 -8.33 -8.63 -4.29 -2.12 -8.06 -6.35 -2.91 -7.08 -4.31 -13.11 -10.11 -10.83
None -0.64 7.65 9.44 8.06 0.70 -0.07 1.72 -0.51 -0.60 -2.68 2.24 7.50 8.21 6.50 -0.63
Nuclear Periphery 2.66 5.22 -5.03 -5.87 -6.49 -7.70 -4.75 -5.76 -2.73 -3.67 -2.01 4.28 -6.63 -5.96 -8.17
Nuclear Periphery Foci -0.50 4.59 -0.14 0.36 -4.16 -1.04 -0.42 -1.03 0.05 -0.56 -0.92 4.66 -0.57 0.40 -5.50
Nucleolus -3.30 1.19 6.83 10.33 5.77 0.28 4.81 5.27 9.19 -0.29 -4.57 2.94 7.86 14.71 4.81
Nucleus -3.56 -9.63 6.11 9.14 14.45 5.00 2.56 11.25 8.63 5.52 -1.68 -8.06 10.09 12.36 17.86
Peroxisomes -0.64 5.81 2.09 1.71 -2.37 -3.31 0.58 1.88 4.51 1.51 -3.15 6.14 2.86 4.57 -2.91
Vacuole -2.77 -2.57 -9.86 -9.42 -9.28 -5.62 -3.00 -9.90 -8.44 0.22 -6.01 -3.78 -13.72 -12.27 -10.63
Vacuole Periphery -2.95 -0.30 -2.92 -2.00 -2.89 -4.93 -1.27 -3.41 -1.63 -3.34 -5.56 -0.47 -4.36 -2.29 -4.32
Standard name
Human Ortholog
Description Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rco1

Rco1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rco1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available