Standard name
Human Ortholog
Description Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.05 0 0.12 0.07 0 0 0 0 0.06 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.98 0.96 0.92 0.95 0.87 0.86 0.83 0.8 0.83 0.81 0.98 0.98 0.97 0.98 0.98 0.97 0.98 0.96 0.94 0.94 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 0.11 0.12 0.14 0.1 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 5 8 3 6 10 0 0 0 0 0 0 0 0 0 3 0 2
Bud 6 0 4 0 9 3 3 8 2 5 4 11 3 2 2 0 0 0 0 1 0 1 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 29 6 11 34 33 22 17 17 19 37 34 52 15 13 17 0 6 1 0 0 1 0 0 0
Cytoplasm 329 120 267 276 421 526 528 528 452 501 455 585 292 437 395 110 163 107 337 106 264 272 221 246
Endoplasmic Reticulum 0 0 3 5 13 20 69 68 64 86 56 85 4 7 10 6 5 3 1 0 5 4 2 5
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 2 0 1 1 1 1 3 1 11 3 21 11 1 0 0 0 0 1 0 0 1 0 0 1
Nucleus 4 0 0 2 3 3 6 5 4 13 3 5 1 1 0 0 0 0 0 0 0 0 2 0
Nuclear Periphery 0 0 0 0 0 0 1 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 13 0 3 3 6 19 4 7 0 1 1 0 2 2 1 0 2 1 3 4
Unique Cell Count 335 120 273 287 456 554 609 612 542 629 551 722 299 447 406 112 166 110 344 110 281 290 241 268
Labelled Cell Count 370 126 287 319 493 576 632 637 567 667 586 767 316 461 425 117 176 114 344 110 281 290 241 268


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.9 50.3 34.3 32.2 28.4 24.4 23.7 25.4 23.7 24.2 22.8 23.2 40.0 40.3 41.5 45.8 48.3 50.1 44.0 44.1 44.3
Std Deviation (1e-4) 8.1 11.1 7.4 6.6 6.6 5.0 4.7 5.6 5.2 5.2 5.0 4.7 8.3 9.2 7.8 13.5 14.1 13.1 10.9 12.1 12.3
Intensity Change (Log2) – – – -0.09 -0.28 -0.49 -0.53 -0.43 -0.54 -0.51 -0.59 -0.56 0.22 0.23 0.27 0.42 0.49 0.55 0.36 0.36 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.4 1.8 0 -1.0 -1.0 -0.4 1.1 1.3 1.8 0.6 -0.8 0.1 0 -0.2 0
Cytoplasm -1.1 -3.1 -1.9 -5.1 -5.2 -6.0 -7.0 -6.2 -6.7 -0.1 0 -0.4 0.3 0.3 -0.3
Endoplasmic Reticulum 0 1.6 2.1 5.1 5.1 5.3 5.8 4.8 5.3 0 0 1.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 30.8927 40.3192 36.4718 35.3111 30.1898 38.111 35.7403 45.1604 41.485 38.2656 38.0118 39.2932 32.4491 41.3635 36.7276 34.696 24.2948 38.0533
Actin 0.0034 0.0006 0.002 0.0124 0.0026 0.0015 0.0033 0.0003 0.0097 0.0027 0.0456 0.0007 0.0027 0.0005 0.0156 0.0007 0.0637 0.0013
Bud 0.0007 0 0.0007 0.0005 0.0007 0.0006 0.0002 0 0.0002 0.0035 0.0002 0.0001 0.0002 0 0.001 0.0002 0.0015 0
Bud Neck 0.0009 0.0005 0.0006 0.0013 0.0013 0.0031 0.0003 0.0003 0.0005 0.0008 0.0007 0.0064 0.0044 0.0005 0.0004 0.0005 0.0021 0.0026
Bud Periphery 0.0006 0 0.0005 0.0008 0.0007 0.0004 0.0002 0 0.0002 0.0049 0.0001 0.0001 0.0002 0 0.0011 0.0004 0.0021 0
Bud Site 0.0012 0.0003 0.0006 0.0015 0.0008 0.0001 0.0002 0.0001 0.0006 0.0009 0.0005 0 0.0089 0 0.005 0.0001 0.0163 0
Cell Periphery 0.001 0.0001 0.0006 0.0006 0.0005 0.0003 0.0002 0.0002 0.0002 0.0005 0.0001 0.0001 0.0003 0 0.0001 0.0002 0.0008 0
Cytoplasm 0.8483 0.992 0.936 0.9187 0.8467 0.9802 0.9177 0.9973 0.9546 0.8955 0.9095 0.9662 0.9143 0.9964 0.9412 0.9265 0.6118 0.9656
Cytoplasmic Foci 0.0277 0.0005 0.0122 0.0237 0.0341 0.0008 0.009 0.0002 0.008 0.0129 0.0083 0.002 0.0162 0.0001 0.0059 0.019 0.0627 0.0018
Eisosomes 0.0001 0 0 0.0007 0 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0008 0
Endoplasmic Reticulum 0.0068 0.0029 0.0049 0.0033 0.0046 0.0073 0.0059 0.0005 0.0014 0.0019 0.0076 0.0049 0.0024 0.0008 0.0025 0.0115 0.0024 0.0043
Endosome 0.0225 0.0001 0.0073 0.0055 0.028 0.0002 0.013 0 0.0024 0.008 0.0103 0.001 0.0078 0 0.0045 0.0044 0.0412 0.0002
Golgi 0.0033 0 0.002 0.0045 0.0185 0.0001 0.0023 0 0.0019 0.0008 0.0017 0.0001 0.0013 0 0.0021 0.0006 0.0307 0.0003
Lipid Particles 0.0124 0.0001 0.0073 0.0107 0.0127 0.0001 0.0041 0 0.008 0.0041 0.0062 0.0003 0.0097 0 0.0024 0.0108 0.0716 0.0049
Mitochondria 0.0119 0 0.0024 0.0057 0.0085 0.0002 0.0026 0 0.0027 0.0175 0.0011 0.0089 0.0073 0 0.0053 0.0025 0.0375 0.0053
None 0.0052 0.0002 0.0067 0.0026 0.0043 0.0003 0.0049 0.0001 0.0016 0.004 0.0014 0.0012 0.0045 0.0001 0.0036 0.0031 0.0035 0.0001
Nuclear Periphery 0.0063 0.0007 0.0017 0.0014 0.0036 0.001 0.0111 0.0002 0.0007 0.009 0.0024 0.0005 0.0017 0.0004 0.0029 0.0074 0.0087 0.0009
Nucleolus 0.0034 0 0.0008 0.0003 0.0007 0.0001 0.0002 0 0.0001 0.0022 0 0.0017 0.0005 0 0.0002 0.0005 0.001 0
Nucleus 0.0139 0.0017 0.0029 0.0024 0.0038 0.0031 0.0052 0.0007 0.0011 0.0038 0.0014 0.0024 0.003 0.001 0.0022 0.0038 0.0021 0.0027
Peroxisomes 0.002 0 0.0023 0.0015 0.0056 0 0.0064 0 0.0036 0.0009 0.001 0.0002 0.009 0 0.0006 0.0008 0.0273 0.0095
Punctate Nuclear 0.0114 0.0001 0.0024 0.0008 0.001 0.0002 0.0061 0 0.0008 0.0061 0.0008 0.0005 0.0033 0 0.0019 0.0017 0.0053 0.0001
Vacuole 0.0134 0.0001 0.0049 0.001 0.0158 0.0003 0.0045 0 0.0013 0.0125 0.0007 0.0022 0.0016 0 0.0009 0.0033 0.0045 0.0001
Vacuole Periphery 0.0036 0 0.0011 0.0003 0.0055 0.0001 0.0026 0 0.0002 0.0076 0.0004 0.0005 0.0006 0 0.0006 0.002 0.0022 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 268.9171 293.2178 222.3799 192.7911 229.836 278.4102 235.7063 179.1326 174.9152 214.9034
Translational Efficiency 2.0614 1.9452 1.692 2.0608 1.9863 2.0086 1.8868 2.2033 1.8108 1.9534

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sec14

Sec14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available