Standard name | |
---|---|
Human Ortholog | |
Description | Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 111 | 71 | 152 | 160 | 170 | 226 | 236 | 186 | 184 | – | 134 | 150 | 172 | 234 | 269 | 76 | 35 | 30 | 21 | 23 | 54 | 40 | 57 | 44 |
Bud | 9 | 6 | 32 | 26 | 23 | 38 | 36 | 49 | 26 | – | 29 | 27 | 12 | 25 | 45 | 9 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 130 | 95 | 122 | 138 | 169 | 201 | 207 | 122 | 177 | – | 142 | 175 | 160 | 221 | 275 | 254 | 160 | 165 | 177 | 108 | 176 | 133 | 173 | 187 |
Cytoplasm | 4 | 8 | 17 | 20 | 32 | 23 | 37 | 41 | 17 | – | 27 | 21 | 10 | 23 | 16 | 28 | 15 | 22 | 0 | 1 | 6 | 0 | 2 | 3 |
Endoplasmic Reticulum | 12 | 8 | 11 | 15 | 4 | 14 | 23 | 30 | 34 | – | 9 | 20 | 26 | 25 | 11 | 12 | 10 | 8 | 5 | 4 | 4 | 1 | 6 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Golgi | 3 | 0 | 5 | 0 | 4 | 1 | 1 | 9 | 0 | – | 0 | 0 | 4 | 3 | 0 | 19 | 17 | 7 | 0 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 54 | 35 | 80 | 171 | 162 | 261 | 366 | 386 | 305 | – | 254 | 327 | 84 | 99 | 215 | 29 | 17 | 13 | 1 | 0 | 3 | 1 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 4 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 11 | 2 | 45 | 34 | 112 | 102 | 120 | 106 | 27 | – | 31 | 32 | 26 | 87 | 53 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | – | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 3 | 0 | 1 | 1 | 3 | 1 | 3 | 0 | 1 | 1 | 0 | 0 | 0 |
Unique Cell Count | 187 | 140 | 224 | 253 | 296 | 400 | 538 | 577 | 414 | 345 | 417 | 247 | 374 | 397 | 316 | 199 | 196 | 209 | 149 | 259 | 184 | 250 | 246 | |
Labelled Cell Count | 335 | 225 | 467 | 565 | 679 | 869 | 1028 | 943 | 771 | 631 | 756 | 494 | 718 | 887 | 432 | 257 | 254 | 209 | 149 | 259 | 184 | 250 | 246 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 9.4 | 8.1 | 7.6 | 8.8 | 8.1 | 7.8 | 7.6 | 6.8 | – | 6.4 | 6.5 | 9.0 | 8.9 | 8.0 | 14.4 | 15.9 | 16.3 | 9.2 | 9.1 | 9.5 |
Std Deviation (1e-4) | 1.2 | 1.4 | 2.6 | 1.4 | 1.7 | 1.4 | 1.4 | 1.6 | 1.2 | – | 1.1 | 1.3 | 1.3 | 1.4 | 1.5 | 3.9 | 3.8 | 4.6 | 1.3 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.11 | -0.01 | -0.07 | -0.1 | -0.27 | – | -0.34 | -0.32 | 0.14 | 0.12 | -0.02 | 0.82 | 0.97 | 1.0 | 0.18 | 0.16 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.1 | -2.4 | -2.8 | -6.0 | -9.2 | -5.7 | – | -6.8 | -7.7 | 0.4 | -1.3 | 0 | -10.2 | -10.4 | -10.8 |
Bud | -1.3 | -2.4 | -1.8 | -3.3 | -2.4 | -3.4 | – | -2.2 | -3.3 | -3.5 | -3.1 | -1.1 | -4.9 | -5.3 | -4.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.6 | -1.0 | -4.1 | -9.2 | -2.8 | – | -3.1 | -3.0 | 2.3 | 1.1 | 3.7 | 6.5 | 5.6 | 6.5 |
Cytoplasm | 0.1 | 1.2 | -0.9 | -0.3 | -0.2 | -1.9 | – | 0.1 | -1.3 | -1.7 | -0.7 | -1.9 | 0.5 | 0 | 1.3 |
Endoplasmic Reticulum | 0.5 | -2.4 | -0.9 | -0.4 | 0.2 | 1.6 | – | -1.5 | -0.1 | 2.3 | 0.9 | -1.4 | -0.6 | 0.1 | -0.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.9 | 0.8 |
Mitochondria | 7.0 | 4.3 | 7.1 | 8.2 | 8.0 | 9.4 | – | 9.0 | 10.7 | -0.4 | -2.4 | 4.4 | -7.6 | -6.6 | -7.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -2.0 | 4.4 | 1.5 | 0.7 | -0.6 | -5.2 | – | -3.8 | -4.6 | -2.9 | 0.9 | -2.2 | -8.3 | -6.5 | -6.4 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.883 | 7.2655 | 7.1967 | 5.4322 | 6.1464 | 7.0255 | 7.0618 | 7.8543 | 7.442 | 6.4105 | 7.179 | 7.2612 | 6.7237 | 6.8945 | 7.5445 | 6.9896 | 6.2693 | 7.1876 |
Actin | 0.0706 | 0.015 | 0.0252 | 0.023 | 0.0651 | 0.008 | 0.0558 | 0.0106 | 0.0137 | 0.0142 | 0.0059 | 0.0088 | 0.0374 | 0.0099 | 0.0336 | 0.0181 | 0.0211 | 0.0061 |
Bud | 0.0009 | 0.0004 | 0.0008 | 0.0011 | 0.0014 | 0.0004 | 0.0013 | 0.0003 | 0.0004 | 0.0006 | 0.0005 | 0.0004 | 0.0013 | 0.0006 | 0.0078 | 0.0137 | 0.0005 | 0.0002 |
Bud Neck | 0.0054 | 0.0071 | 0.0086 | 0.0064 | 0.0126 | 0.0132 | 0.0047 | 0.0047 | 0.0089 | 0.0148 | 0.0072 | 0.0024 | 0.0045 | 0.0044 | 0.0063 | 0.0094 | 0.0037 | 0.0052 |
Bud Periphery | 0.0059 | 0.0029 | 0.0086 | 0.0094 | 0.0257 | 0.0085 | 0.008 | 0.0023 | 0.0052 | 0.006 | 0.0102 | 0.0075 | 0.012 | 0.0031 | 0.0079 | 0.0281 | 0.0088 | 0.0035 |
Bud Site | 0.0061 | 0.1154 | 0.0267 | 0.006 | 0.0165 | 0.0034 | 0.0106 | 0.0741 | 0.014 | 0.0052 | 0.0026 | 0.0009 | 0.0363 | 0.0656 | 0.0116 | 0.02 | 0.0048 | 0.001 |
Cell Periphery | 0.4652 | 0.6076 | 0.6869 | 0.6391 | 0.7209 | 0.736 | 0.5618 | 0.6477 | 0.7399 | 0.7592 | 0.7004 | 0.8222 | 0.5324 | 0.5803 | 0.6692 | 0.7408 | 0.5785 | 0.7904 |
Cytoplasm | 0.0224 | 0.0499 | 0.0422 | 0.0652 | 0.0363 | 0.0256 | 0.0286 | 0.0518 | 0.0266 | 0.0495 | 0.018 | 0.0239 | 0.0199 | 0.0881 | 0.0393 | 0.0371 | 0.0211 | 0.0204 |
Cytoplasmic Foci | 0.0052 | 0.0005 | 0.001 | 0.0154 | 0.0005 | 0.0028 | 0.0065 | 0.0002 | 0.0006 | 0.0056 | 0.0017 | 0.0007 | 0.0026 | 0.0004 | 0.0051 | 0.0004 | 0.0012 | 0.0001 |
Eisosomes | 0.2735 | 0.1834 | 0.1675 | 0.066 | 0.0897 | 0.1558 | 0.2353 | 0.2016 | 0.1728 | 0.0383 | 0.1877 | 0.1178 | 0.3049 | 0.237 | 0.1862 | 0.1156 | 0.2363 | 0.1648 |
Endoplasmic Reticulum | 0.0107 | 0.0025 | 0.006 | 0.0107 | 0.0058 | 0.0082 | 0.0055 | 0.0023 | 0.0032 | 0.0073 | 0.0044 | 0.0032 | 0.0027 | 0.0025 | 0.0024 | 0.0091 | 0.0055 | 0.0046 |
Endosome | 0.0161 | 0.0001 | 0.0043 | 0.0289 | 0.0008 | 0.0054 | 0.0083 | 0.0001 | 0.0007 | 0.0319 | 0.0126 | 0.0024 | 0.0078 | 0 | 0.0032 | 0 | 0.0103 | 0.0001 |
Golgi | 0.0058 | 0.0008 | 0.0042 | 0.0133 | 0.0038 | 0.0047 | 0.0078 | 0.0002 | 0.0031 | 0.0179 | 0.0097 | 0.0027 | 0.0025 | 0.0005 | 0.0095 | 0.0012 | 0.0252 | 0.0005 |
Lipid Particles | 0.0041 | 0.0006 | 0.001 | 0.0333 | 0.0007 | 0.0031 | 0.014 | 0.0002 | 0.0007 | 0.0129 | 0.0033 | 0.002 | 0.0061 | 0.0006 | 0.0007 | 0.0006 | 0.0058 | 0.0004 |
Mitochondria | 0.0321 | 0.0106 | 0.0106 | 0.0181 | 0.0162 | 0.0123 | 0.0139 | 0.0014 | 0.0064 | 0.0241 | 0.0022 | 0.0014 | 0.002 | 0.0001 | 0.0035 | 0.0001 | 0.0115 | 0.0001 |
None | 0.0059 | 0.0014 | 0.0018 | 0.0022 | 0.0012 | 0.0012 | 0.0089 | 0.0012 | 0.0013 | 0.001 | 0.0297 | 0.0007 | 0.0021 | 0.0048 | 0.0024 | 0.0014 | 0.0515 | 0.001 |
Nuclear Periphery | 0.0409 | 0.0004 | 0.0016 | 0.0045 | 0.0006 | 0.0011 | 0.0068 | 0.0004 | 0.0004 | 0.0032 | 0.0012 | 0.0005 | 0.0058 | 0.0006 | 0.0004 | 0.0008 | 0.0046 | 0.0007 |
Nucleolus | 0.0006 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0011 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.004 | 0.0001 | 0.0001 | 0.0002 | 0.0012 | 0.0001 |
Nucleus | 0.0051 | 0.0001 | 0.0007 | 0.0153 | 0.0001 | 0.0007 | 0.0078 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0029 | 0.0002 | 0.0001 | 0.0005 | 0.0026 | 0.0001 |
Peroxisomes | 0.0013 | 0.0001 | 0.0002 | 0.0103 | 0.0003 | 0.0027 | 0.0087 | 0 | 0.0002 | 0.0025 | 0.0003 | 0.0007 | 0.0012 | 0 | 0.0092 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0.0124 | 0 | 0.0002 | 0.0039 | 0 | 0.0037 | 0.0016 | 0 | 0 | 0.0007 | 0.0005 | 0.0004 | 0.0092 | 0.0001 | 0.0004 | 0 | 0.0027 | 0.0001 |
Vacuole | 0.0041 | 0.001 | 0.0016 | 0.0232 | 0.0017 | 0.0017 | 0.0027 | 0.0006 | 0.0014 | 0.003 | 0.0012 | 0.0011 | 0.0018 | 0.0008 | 0.0009 | 0.0027 | 0.002 | 0.0008 |
Vacuole Periphery | 0.0056 | 0 | 0.0002 | 0.0034 | 0.0002 | 0.0004 | 0.0008 | 0 | 0.0002 | 0.0015 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.8832 | 17.791 | 13.4556 | 23.2441 | 23.0744 | 17.9783 | 20.2371 | 20.6631 | 21.0235 | 27.1439 |
Translational Efficiency | 1.3958 | 1.3152 | 1.5524 | 1.1311 | 1.2855 | 1.4156 | 0.9292 | 1.1093 | 1.2479 | 1.1529 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|