Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 2 | 1 | 4 | 4 | 8 | 2 | 5 | 8 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 3 | 8 | 7 | 17 | 8 | 13 | 21 | 8 | 7 | 8 | 15 | 27 | 41 | 4 | 4 | 9 | 3 | 3 | 0 | 4 | 1 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4 | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 2 | 3 | 3 | 1 | 2 | 1 | 2 | 9 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 5 | 1 | 0 |
Mitochondria | 12 | 2 | 32 | 41 | 8 | 64 | 61 | 85 | 54 | 85 | 78 | 111 | 12 | 3 | 1 | 2 | 2 | 4 | 0 | 1 | 3 | 1 | 2 | 3 |
Nucleus | 17 | 32 | 59 | 87 | 136 | 116 | 192 | 267 | 127 | 156 | 134 | 183 | 188 | 170 | 173 | 26 | 12 | 28 | 37 | 59 | 57 | 44 | 125 | 155 |
Nuclear Periphery | 0 | 0 | 1 | 2 | 3 | 2 | 4 | 5 | 5 | 3 | 4 | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 3 | 7 | 18 | 12 | 47 | 59 | 110 | 48 | 79 | 84 | 93 | 32 | 26 | 38 | 9 | 1 | 1 | 4 | 7 | 7 | 13 | 37 | 52 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 1 | 6 |
Vac/Vac Membrane | 6 | 18 | 2 | 3 | 5 | 6 | 5 | 11 | 10 | 3 | 2 | 2 | 3 | 4 | 6 | 5 | 3 | 7 | 11 | 10 | 5 | 4 | 11 | 20 |
Unique Cell Count | 35 | 62 | 73 | 122 | 154 | 191 | 253 | 369 | 201 | 224 | 219 | 270 | 222 | 205 | 244 | 41 | 20 | 49 | 72 | 95 | 82 | 80 | 189 | 260 |
Labelled Cell Count | 41 | 67 | 106 | 164 | 176 | 259 | 336 | 507 | 270 | 340 | 320 | 413 | 257 | 232 | 268 | 50 | 25 | 56 | 72 | 95 | 82 | 80 | 189 | 260 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.7 | 5.2 | 5.7 | 6.7 | 5.1 | 5.3 | 5.4 | 7.1 | 5.2 | 5.2 | 5.0 | 6.9 | 7.1 | 7.0 | 9.0 | 8.9 | 8.4 | 8.8 | 7.5 | 7.5 |
Std Deviation (1e-4) | 3.2 | 1.5 | 2.1 | 2.9 | 3.2 | 2.1 | 2.0 | 2.2 | 5.4 | 2.1 | 2.0 | 1.7 | 1.5 | 2.0 | 2.2 | 2.3 | 2.5 | 2.8 | 4.2 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.36 | -0.05 | 0.03 | 0.05 | 0.44 | -0.0 | -0.01 | -0.06 | 0.39 | 0.44 | 0.42 | 0.79 | 0.76 | 0.68 | 0.74 | 0.51 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.3 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 2.1 | 2.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.4 | -7.1 | -1.6 | -3.3 | -3.7 | -2.7 | -0.9 | -1.3 | -0.4 | -8.0 | -9.4 | -10.7 | -4.4 | -2.8 | -4.2 |
Nucleus | -1.5 | 1.5 | -3.1 | -0.9 | -1.5 | -2.8 | -1.9 | -3.1 | -2.2 | 0.8 | 0.4 | -1.7 | -2.0 | -1.9 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.0 | -0.5 | 2.7 | 2.6 | 3.6 | 2.6 | 4.2 | 4.6 | 4.1 | 1.1 | 0.7 | 1.3 | 1.8 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7867 | 0.5384 | 0.7401 | 1.2074 | 1.646 | 0.4679 | 1.3802 | 0.076 | 0.1151 | -0.0288 | 1.6504 | 0.3173 | 3.1309 | 1.6383 | 1.7121 | 2.2343 | 3.987 | 1.7724 |
Actin | 0.076 | 0.0002 | 0.0323 | 0.0193 | 0.017 | 0.0001 | 0.0945 | 0.0021 | 0.0306 | 0.0035 | 0.1054 | 0.0029 | 0.0782 | 0.0001 | 0.0104 | 0.0476 | 0.197 | 0.0008 |
Bud | 0.0008 | 0.0001 | 0.0011 | 0.0012 | 0.0008 | 0.0003 | 0.001 | 0.0016 | 0.0013 | 0.0002 | 0.0046 | 0.0003 | 0.0009 | 0.0002 | 0.0004 | 0.0017 | 0.0013 | 0.0001 |
Bud Neck | 0.0014 | 0.0004 | 0.0054 | 0.1068 | 0.0004 | 0.0073 | 0.0054 | 0.0007 | 0.0012 | 0.0005 | 0.003 | 0.0024 | 0.0129 | 0.0003 | 0.0127 | 0.0006 | 0.0212 | 0.0036 |
Bud Periphery | 0.0013 | 0 | 0.0011 | 0.0015 | 0.0019 | 0.0005 | 0.0019 | 0.0003 | 0.0015 | 0.0004 | 0.0093 | 0.0009 | 0.0017 | 0.0001 | 0.0007 | 0.0024 | 0.0035 | 0.0003 |
Bud Site | 0.0026 | 0.0008 | 0.0119 | 0.0224 | 0.0018 | 0.0006 | 0.0036 | 0.0039 | 0.0144 | 0.0009 | 0.0138 | 0.0005 | 0.0083 | 0.0022 | 0.006 | 0.0072 | 0.0028 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0003 | 0.0009 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0 |
Cytoplasm | 0.0245 | 0.0149 | 0.0066 | 0.0026 | 0.006 | 0.0038 | 0.0232 | 0.0084 | 0.0027 | 0.0094 | 0.0095 | 0.0018 | 0.0053 | 0.016 | 0.0038 | 0.0036 | 0.0033 | 0.005 |
Cytoplasmic Foci | 0.0303 | 0.0059 | 0.0448 | 0.0971 | 0.064 | 0.0003 | 0.0412 | 0.0039 | 0.0124 | 0.0064 | 0.0275 | 0.0042 | 0.0673 | 0.0004 | 0.0029 | 0.0068 | 0.0169 | 0.0003 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0008 | 0 | 0.0001 | 0.0012 | 0.001 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0003 | 0.0016 | 0.0001 |
Endoplasmic Reticulum | 0.0129 | 0.0009 | 0.0172 | 0.0005 | 0.0021 | 0.0002 | 0.0048 | 0.0003 | 0.002 | 0.0007 | 0.0034 | 0.001 | 0.0029 | 0.0005 | 0.0003 | 0.0005 | 0.0134 | 0.0004 |
Endosome | 0.0397 | 0.0011 | 0.01 | 0.0544 | 0.0763 | 0.0009 | 0.0543 | 0.0012 | 0.0161 | 0.038 | 0.0209 | 0.0069 | 0.0393 | 0.0004 | 0.01 | 0.0086 | 0.0346 | 0.0011 |
Golgi | 0.0116 | 0.0005 | 0.0047 | 0.0547 | 0.0292 | 0.0001 | 0.0301 | 0.0016 | 0.0153 | 0.0372 | 0.0216 | 0.004 | 0.0308 | 0 | 0.0019 | 0.0114 | 0.0145 | 0.0006 |
Lipid Particles | 0.0167 | 0.0013 | 0.0087 | 0.0335 | 0.0734 | 0 | 0.0502 | 0.0004 | 0.0043 | 0.0394 | 0.0514 | 0.0205 | 0.0878 | 0 | 0.0038 | 0.0254 | 0.0353 | 0.0026 |
Mitochondria | 0.0341 | 0.0004 | 0.0013 | 0.0111 | 0.2876 | 0.0003 | 0.0453 | 0.0007 | 0.0094 | 0.0471 | 0.158 | 0.0044 | 0.024 | 0.0004 | 0.0019 | 0.0392 | 0.0069 | 0.0057 |
None | 0.0532 | 0.0389 | 0.0026 | 0.0005 | 0.0078 | 0.0003 | 0.0092 | 0.0095 | 0.0009 | 0.007 | 0.0027 | 0.0006 | 0.0045 | 0.0278 | 0.0014 | 0.054 | 0.0143 | 0.0147 |
Nuclear Periphery | 0.0355 | 0.0082 | 0.0056 | 0.0011 | 0.0114 | 0.0035 | 0.0216 | 0.0106 | 0.011 | 0.0147 | 0.1305 | 0.0191 | 0.0086 | 0.0093 | 0.0026 | 0.0026 | 0.0854 | 0.0054 |
Nucleolus | 0.0098 | 0.0221 | 0.0152 | 0.0038 | 0.0028 | 0.0633 | 0.0133 | 0.0198 | 0.0099 | 0.0146 | 0.0086 | 0.0515 | 0.0104 | 0.0196 | 0.0182 | 0.007 | 0.0296 | 0.0594 |
Nucleus | 0.5859 | 0.8528 | 0.798 | 0.5504 | 0.2523 | 0.9082 | 0.5401 | 0.891 | 0.8311 | 0.7414 | 0.3193 | 0.8227 | 0.5325 | 0.8941 | 0.8964 | 0.7633 | 0.374 | 0.8724 |
Peroxisomes | 0.0108 | 0.0051 | 0.0203 | 0.0302 | 0.0173 | 0 | 0.0237 | 0.001 | 0.0103 | 0.0071 | 0.0718 | 0.0028 | 0.0436 | 0 | 0.0015 | 0.0115 | 0.0583 | 0.0002 |
Punctate Nuclear | 0.047 | 0.0454 | 0.0086 | 0.0052 | 0.1269 | 0.0082 | 0.0221 | 0.0423 | 0.0226 | 0.0258 | 0.0314 | 0.0499 | 0.0298 | 0.0277 | 0.0187 | 0.0052 | 0.0759 | 0.0264 |
Vacuole | 0.0023 | 0.0009 | 0.0038 | 0.0017 | 0.0088 | 0.0018 | 0.0083 | 0.0004 | 0.0017 | 0.0013 | 0.0027 | 0.0016 | 0.0055 | 0.0006 | 0.0043 | 0.0005 | 0.0038 | 0.0005 |
Vacuole Periphery | 0.0026 | 0.0002 | 0.0003 | 0.0007 | 0.0115 | 0.0002 | 0.0048 | 0.0001 | 0.001 | 0.003 | 0.0032 | 0.0019 | 0.0039 | 0.0001 | 0.0017 | 0.0005 | 0.0057 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.5387 | 12.4542 | 12.9382 | 15.0199 | 25.2886 | 8.3545 | 16.3702 | 11.8987 | 13.753 | 17.184 |
Translational Efficiency | 2.3256 | 1.4362 | 1.5712 | 0.9972 | 0.8799 | 3.8757 | 1.7056 | 2.0449 | 1.7509 | 1.683 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1407 | 905 | 878 | 167 | 971 | 1262 | 1106 | 1667 | 2378 | 2167 | 1984 | 1834 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 702.91 | 725.84 | 909.22 | 893.76 | 653.31 | 779.60 | 836.00 | 810.80 | 682.66 | 757.15 | 868.40 | 818.35 |
Standard Deviation | 97.75 | 102.41 | 119.62 | 121.45 | 85.02 | 102.12 | 125.66 | 115.02 | 95.92 | 105.62 | 128.29 | 118.05 |
Intensity Change Log 2 | — | 0.046312 | 0.371289 | 0.346547 | — | 0.254966 | 0.355735 | 0.311578 | — | 0.150594 | 0.363818 | 0.329808 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000265 | 0.001100 | 0.001130 | 0.002126 | 0.000371 | 0.001245 | 0.001065 | 0.000949 | 0.000308 | 0.001185 | 0.001094 | 0.001056 |
Bud Neck | 0.007866 | 0.013266 | 0.014683 | 0.006836 | 0.004882 | 0.009986 | 0.016173 | 0.016153 | 0.006648 | 0.011356 | 0.015514 | 0.015305 |
Bud Site | 0.001698 | 0.004093 | 0.001671 | 0.007452 | 0.000853 | 0.002745 | 0.006491 | 0.006108 | 0.001353 | 0.003308 | 0.004358 | 0.006230 |
Cell Periphery | 0.000148 | 0.000180 | 0.000078 | 0.000218 | 0.000163 | 0.000120 | 0.000166 | 0.000120 | 0.000154 | 0.000145 | 0.000127 | 0.000129 |
Cytoplasm | 0.101444* | 0.061936 | 0.006092 | 0.024514 | 0.055388 | 0.034665 | 0.028301 | 0.040095 | 0.082638 | 0.046054 | 0.018473 | 0.038676 |
Cytoplasmic Foci | 0.016656 | 0.013073 | 0.000121 | 0.002132 | 0.015933 | 0.013186 | 0.000915 | 0.001664 | 0.016361 | 0.013139 | 0.000564 | 0.001707 |
Eisosomes | 0.000048 | 0.000065 | 0.000034 | 0.000083 | 0.000057 | 0.000055 | 0.000037 | 0.000041 | 0.000052 | 0.000059 | 0.000036 | 0.000045 |
Endoplasmic Reticulum | 0.002741 | 0.006907 | 0.006202 | 0.010280 | 0.003048 | 0.004264 | 0.004498 | 0.005316 | 0.002866 | 0.005367 | 0.005252 | 0.005768 |
Endosome | 0.002750 | 0.003745 | 0.000410 | 0.002216 | 0.001914 | 0.003659 | 0.001161 | 0.001798 | 0.002409 | 0.003695 | 0.000829 | 0.001836 |
Golgi | 0.001825 | 0.000962 | 0.000140 | 0.000843 | 0.000514 | 0.002117 | 0.001438 | 0.000717 | 0.001290 | 0.001634 | 0.000864 | 0.000728 |
Lipid Particles | 0.004841 | 0.004676 | 0.000379 | 0.001367 | 0.007164 | 0.005901 | 0.000552 | 0.001049 | 0.005789 | 0.005390 | 0.000475 | 0.001078 |
Mitochondria | 0.003312 | 0.006378 | 0.001489 | 0.009297 | 0.004378 | 0.015317 | 0.002318 | 0.002451 | 0.003748 | 0.011584 | 0.001951 | 0.003075 |
Mitotic Spindle | 0.015015 | 0.009699 | 0.002816 | 0.037279 | 0.010001 | 0.018650 | 0.016873 | 0.020157 | 0.012968 | 0.014912 | 0.010652 | 0.021716 |
None | 0.016744 | 0.015463 | 0.004033 | 0.028738 | 0.018413 | 0.007919 | 0.009195 | 0.009859 | 0.017426 | 0.011070 | 0.006910 | 0.011578 |
Nuclear Periphery | 0.005669 | 0.004122 | 0.001138 | 0.001621 | 0.004907 | 0.005033 | 0.000984 | 0.001905 | 0.005358 | 0.004653 | 0.001052 | 0.001879 |
Nuclear Periphery Foci | 0.002071 | 0.003581 | 0.000571 | 0.001216 | 0.002395 | 0.001913 | 0.000693 | 0.001729 | 0.002203 | 0.002610 | 0.000639 | 0.001682 |
Nucleolus | 0.019157 | 0.031714 | 0.016472 | 0.032695 | 0.037674 | 0.036858 | 0.020413 | 0.023965 | 0.026718 | 0.034710 | 0.018669 | 0.024760 |
Nucleus | 0.788002* | 0.807153* | 0.938905* | 0.807926* | 0.823953* | 0.821857* | 0.883831* | 0.858220* | 0.802682* | 0.815716* | 0.908203* | 0.853640* |
Peroxisomes | 0.001801 | 0.003290 | 0.000313 | 0.003791 | 0.003023 | 0.004353 | 0.000504 | 0.000914 | 0.002300 | 0.003909 | 0.000419 | 0.001176 |
Vacuole | 0.007520 | 0.007522 | 0.003155 | 0.018864 | 0.004499 | 0.008288 | 0.003968 | 0.006190 | 0.006286 | 0.007968 | 0.003608 | 0.007344 |
Vacuole Periphery | 0.000424 | 0.001076 | 0.000167 | 0.000507 | 0.000470 | 0.001868 | 0.000424 | 0.000601 | 0.000443 | 0.001537 | 0.000310 | 0.000593 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.34 | -15.24 | -8.16 | -4.51 | -4.10 | -1.42 | -12.21 | -11.30 | -0.22 | 1.95 | -2.69 | -19.33 | -15.25 | -0.61 | 0.86 |
Bud Neck | -3.58 | -9.39 | -2.91 | 0.80 | 5.50 | -4.73 | -12.04 | -10.44 | -5.80 | 1.94 | -5.16 | -14.50 | -9.60 | -5.05 | 2.48 |
Bud Site | -3.31 | -2.82 | -3.72 | -1.81 | -3.07 | -4.31 | -5.76 | -6.74 | -2.86 | 0.58 | -4.89 | -5.92 | -6.47 | -2.85 | -1.21 |
Cell Periphery | -2.20 | 5.29 | -1.45 | 0.12 | -3.83 | 1.62 | 1.22 | 2.75 | -0.12 | 1.54 | 0.38 | 3.22 | 2.65 | 1.14 | -0.97 |
Cytoplasm | 6.34 | 19.80 | 9.82 | 4.59 | -3.64 | 3.78 | 8.13 | 4.84 | 1.40 | -4.50 | 8.71 | 19.78 | 12.42 | 4.47 | -7.91 |
Cytoplasmic Foci | 1.57 | 12.23 | 10.80 | 6.68 | -3.26 | 1.81 | 9.62 | 9.03 | 8.54 | -1.93 | 2.39 | 15.42 | 13.87 | 10.64 | -3.01 |
Eisosomes | -3.50 | 4.21 | -4.89 | -3.10 | -6.22 | 0.55 | 3.99 | 2.78 | 2.08 | -1.39 | -2.05 | 5.54 | 0.69 | 2.50 | -4.15 |
Endoplasmic Reticulum | -7.43 | -9.79 | -9.06 | -4.97 | -5.38 | -2.77 | -7.95 | -10.10 | -4.63 | -1.87 | -6.66 | -12.06 | -12.31 | -3.77 | -2.06 |
Endosome | -0.61 | 8.64 | 1.93 | 1.83 | -4.78 | -2.14 | 3.73 | 1.70 | 2.97 | -2.56 | -1.64 | 8.48 | 4.19 | 3.98 | -4.84 |
Golgi | 0.91 | 2.08 | 1.53 | 1.37 | -1.92 | -2.44 | -0.49 | 0.19 | 2.46 | 0.55 | -0.77 | 1.09 | 1.48 | 2.65 | 0.17 |
Lipid Particles | 0.05 | 10.83 | 5.96 | 4.95 | -4.64 | 1.66 | 9.54 | 8.79 | 7.80 | -4.16 | 0.84 | 14.15 | 12.13 | 10.05 | -6.05 |
Mitochondria | -2.25 | 3.20 | -0.31 | 0.88 | -0.93 | -5.28 | 2.45 | 2.24 | 6.57 | -0.72 | -6.00 | 3.55 | 1.86 | 6.87 | -2.11 |
Mitotic Spindle | 1.69 | 3.12 | -1.29 | -1.87 | -2.28 | -2.71 | -3.28 | -3.95 | -1.14 | -0.09 | -0.78 | -0.80 | -3.28 | -2.67 | -2.50 |
None | 0.60 | 6.82 | 0.75 | 0.41 | -2.61 | 4.99 | 5.86 | 5.13 | 0.26 | -1.15 | 4.03 | 8.51 | 5.53 | 2.13 | -3.55 |
Nuclear Periphery | 3.59 | 9.01 | 6.08 | 2.67 | -2.75 | -0.03 | 4.90 | 3.18 | 4.58 | -8.81 | 2.24 | 10.03 | 7.34 | 5.62 | -8.50 |
Nuclear Periphery Foci | -1.78 | 3.52 | 1.19 | 2.81 | -2.23 | 1.00 | 3.64 | 0.46 | -0.45 | -3.12 | -0.62 | 4.97 | 0.35 | 0.88 | -3.48 |
Nucleolus | -4.43 | -1.31 | 0.35 | 3.22 | 1.10 | 0.18 | 3.85 | 3.48 | 3.67 | -0.70 | -3.89 | 1.39 | -0.04 | 3.62 | -1.34 |
Nucleus | -2.11 | -16.05 | -2.23 | -0.96 | 5.31 | 0.57 | -5.37 | -3.27 | -4.14 | 2.79 | -1.63 | -14.32 | -6.84 | -5.15 | 7.06 |
Peroxisomes | -3.04 | 6.13 | 0.35 | 2.31 | -1.48 | -1.62 | 5.13 | 4.65 | 6.43 | -1.33 | -3.71 | 7.52 | 5.47 | 7.68 | -2.39 |
Vacuole | 1.73 | 3.69 | -3.39 | -3.94 | -4.50 | -3.60 | -1.30 | -4.16 | 0.44 | -2.70 | -0.05 | 2.99 | -1.44 | -1.49 | -4.84 |
Vacuole Periphery | -2.33 | 4.42 | 0.13 | 2.35 | -3.40 | -3.04 | 1.56 | 0.21 | 2.98 | -0.64 | -3.74 | 3.24 | 0.02 | 3.40 | -1.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|