Standard name | |
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Human Ortholog | |
Description | Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 5 | – | 0 | 0 | 3 | 1 | – | 1 | 1 | 2 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 3 | 6 | 8 |
Bud Neck | 0 | 2 | – | 0 | 3 | 2 | 4 | – | 3 | 2 | 4 | 3 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud Site | 2 | 4 | – | 0 | 1 | 8 | 1 | – | 2 | 10 | 6 | 9 | 0 | 1 | 1 | 1 | 1 | 5 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 6 | – | 1 | 6 | 3 | 7 | – | 8 | 9 | 7 | 7 | 16 | 40 | 68 | 8 | 11 | 6 | 1 | 1 | 0 | 0 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 17 | 8 | – | 3 | 4 | 2 | 7 | – | 5 | 2 | 8 | 2 | 30 | 44 | 55 | 38 | 37 | 42 | 3 | 9 | 0 | 8 | 14 | 14 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 2 |
Mitochondria | 4 | 22 | – | 0 | 1 | 1 | 1 | – | 5 | 5 | 8 | 8 | 2 | 5 | 2 | 5 | 9 | 10 | 0 | 2 | 0 | 0 | 2 | 5 |
Nucleus | 1 | 8 | – | 4 | 5 | 4 | 6 | – | 6 | 12 | 6 | 7 | 9 | 6 | 12 | 0 | 2 | 3 | 0 | 1 | 0 | 5 | 8 | 3 |
Nuclear Periphery | 0 | 1 | – | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 57 | 128 | – | 10 | 46 | 32 | 62 | – | 33 | 64 | 43 | 55 | 132 | 217 | 234 | 66 | 59 | 73 | 2 | 11 | 0 | 8 | 18 | 7 |
Peroxisomes | 7 | 7 | – | 0 | 1 | 0 | 10 | – | 3 | 0 | 5 | 2 | 9 | 14 | 15 | 1 | 3 | 3 | 0 | 3 | 0 | 1 | 2 | 1 |
SpindlePole | 63 | 136 | – | 11 | 99 | 40 | 116 | – | 54 | 92 | 61 | 92 | 161 | 248 | 245 | 76 | 68 | 86 | 89 | 173 | 17 | 186 | 260 | 204 |
Vac/Vac Membrane | 0 | 6 | – | 1 | 2 | 1 | 1 | – | 2 | 0 | 1 | 0 | 1 | 3 | 5 | 11 | 7 | 18 | 0 | 2 | 0 | 4 | 3 | 5 |
Unique Cell Count | 87 | 204 | 18 | 115 | 54 | 146 | 81 | 126 | 101 | 132 | 220 | 361 | 404 | 121 | 109 | 142 | 103 | 217 | 21 | 229 | 329 | 270 | ||
Labelled Cell Count | 153 | 334 | 32 | 168 | 96 | 218 | 123 | 197 | 152 | 189 | 361 | 581 | 641 | 209 | 202 | 252 | 103 | 217 | 21 | 229 | 329 | 270 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 6.4 | – | 9.3 | 8.1 | 5.2 | 7.0 | – | 7.1 | 6.0 | 8.1 | 6.0 | 7.1 | 6.9 | 7.4 | 9.1 | 9.9 | 9.1 | 6.3 | 6.4 | 6.6 |
Std Deviation (1e-4) | 1.1 | 1.6 | – | 2.9 | 2.1 | 1.4 | 1.7 | – | 3.1 | 1.5 | 3.4 | 1.6 | 1.7 | 1.7 | 1.8 | 2.1 | 2.0 | 1.7 | 1.4 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6432 | 1.8993 | 2.0574 | 2.2959 | 1.2985 | 1.6184 | 3.3424 | 2.6338 | 3.3327 | 3.2141 | 2.7623 | 2.9042 | 4.8698 | 5.6725 | 5.177 | 5.6425 | 4.4817 | 4.6334 |
Actin | 0.0051 | 0 | 0 | 0 | 0 | 0.0001 | 0.036 | 0 | 0.0007 | 0.0272 | 0.0163 | 0.0037 | 0.0089 | 0 | 0.0072 | 0 | 0.0008 | 0.001 |
Bud | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0004 | 0.0003 | 0.0001 | 0.0019 | 0.0001 | 0.0002 | 0.0009 | 0.0009 | 0.0002 | 0.0009 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0001 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0023 | 0.0011 | 0.0005 | 0.0011 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0041 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0013 | 0.0001 | 0.0009 | 0 | 0.004 | 0.0001 |
Cytoplasmic Foci | 0.0127 | 0.0043 | 0.0034 | 0.024 | 0.0189 | 0.0034 | 0.034 | 0.0093 | 0.0083 | 0.0092 | 0.0362 | 0.0103 | 0.007 | 0.002 | 0.0064 | 0.0088 | 0.0207 | 0.0019 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0109 | 0 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.001 | 0 | 0.0004 | 0 | 0.0005 | 0.0002 |
Endosome | 0.0025 | 0 | 0.0001 | 0.0013 | 0.0006 | 0.0001 | 0.0173 | 0.0002 | 0.0045 | 0.0034 | 0.0031 | 0.0021 | 0.0055 | 0 | 0.0027 | 0.0001 | 0.0058 | 0.0028 |
Golgi | 0.0012 | 0.0001 | 0.0001 | 0.0006 | 0.0007 | 0.0001 | 0.0062 | 0.0002 | 0.0031 | 0.0058 | 0.0051 | 0.0084 | 0.0018 | 0.0001 | 0.0005 | 0.0002 | 0.0011 | 0.0009 |
Lipid Particles | 0.0065 | 0.0004 | 0.002 | 0.0011 | 0.0192 | 0.0024 | 0.0468 | 0.0008 | 0.007 | 0.0041 | 0.0469 | 0.0194 | 0.0059 | 0.0003 | 0.0022 | 0.001 | 0.0183 | 0.0033 |
Mitochondria | 0.0012 | 0 | 0 | 0 | 0 | 0.0002 | 0.0034 | 0 | 0.0036 | 0.006 | 0.0017 | 0.0015 | 0.0009 | 0 | 0.0004 | 0 | 0.0007 | 0.002 |
None | 0.0018 | 0 | 0 | 0 | 0 | 0.0001 | 0.0032 | 0 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0034 | 0 | 0.001 | 0 | 0.043 | 0.0002 |
Nuclear Periphery | 0.0018 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0242 | 0.0004 | 0.0032 | 0.002 | 0.003 | 0.0004 | 0.0113 | 0.0008 | 0.0009 | 0.0005 | 0.0068 | 0.0057 |
Nucleolus | 0.0261 | 0.0513 | 0.0242 | 0.0018 | 0.002 | 0.0126 | 0.019 | 0.0862 | 0.0475 | 0.0022 | 0.0067 | 0.005 | 0.0029 | 0.0566 | 0.0246 | 0.0012 | 0.0127 | 0.0095 |
Nucleus | 0.0054 | 0.0062 | 0.0075 | 0.0006 | 0.0003 | 0.0026 | 0.0095 | 0.0912 | 0.0161 | 0.0014 | 0.0009 | 0.0009 | 0.0017 | 0.0208 | 0.0037 | 0.0005 | 0.0093 | 0.0029 |
Peroxisomes | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0007 | 0.0001 | 0.0279 | 0.0004 | 0.0062 | 0.0011 | 0.0259 | 0.0007 | 0.0019 | 0 | 0.0005 | 0.0002 | 0.0012 | 0.0008 |
Punctate Nuclear | 0.9326 | 0.9371 | 0.9617 | 0.9677 | 0.9572 | 0.9777 | 0.75 | 0.809 | 0.8972 | 0.9324 | 0.8508 | 0.9465 | 0.9446 | 0.9189 | 0.9475 | 0.9873 | 0.873 | 0.9679 |
Vacuole | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0014 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0005 | 0.0002 |
Vacuole Periphery | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.5935 | 6.1456 | 4.5603 | 8.8786 | 7.7993 | 10.3065 | 11.2626 | 3.1455 | 8.2095 | 13.7062 |
Translational Efficiency | 2.7769 | 2.9085 | 1.9411 | 1.4234 | 3.0147 | 2.4007 | 1.6788 | 4.5571 | 1.5714 | 1.6048 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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