Standard name | |
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Human Ortholog | |
Description | Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 11 | 3 | 15 | 9 | 27 | 39 | – | 7 | 23 | 27 | 45 | 12 | 43 | 63 | 4 | – | 1 | 3 | 2 | 2 | 8 | 27 | 33 |
Endoplasmic Reticulum | 7 | 5 | 0 | 11 | 0 | 0 | 0 | – | 3 | 4 | 5 | 1 | 5 | 5 | 5 | 1 | – | 0 | 9 | 5 | 7 | 2 | 10 | 12 |
Endosome | 1 | 1 | 1 | 0 | 5 | 4 | 11 | – | 0 | 3 | 0 | 4 | 0 | 2 | 4 | 1 | – | 1 | 3 | 0 | 0 | 0 | 7 | 8 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 1 | 1 | 0 | 0 | 2 | 0 |
Mitochondria | 55 | 4 | 0 | 3 | 6 | 15 | 44 | – | 40 | 41 | 157 | 125 | 0 | 0 | 1 | 1 | – | 3 | 14 | 10 | 1 | 10 | 31 | 39 |
Nucleus | 6 | 14 | 1 | 12 | 29 | 52 | 98 | – | 45 | 65 | 73 | 112 | 13 | 27 | 41 | 1 | – | 0 | 1 | 3 | 0 | 3 | 24 | 25 |
Nuclear Periphery | 46 | 21 | 0 | 1 | 22 | 34 | 36 | – | 24 | 32 | 38 | 33 | 22 | 50 | 45 | 4 | – | 3 | 135 | 119 | 25 | 33 | 62 | 64 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 3 | 0 | 3 | 2 | 0 | – | 0 | 4 | 1 | 0 | 1 | 9 | 5 |
Vac/Vac Membrane | 101 | 121 | 17 | 42 | 108 | 88 | 124 | – | 25 | 60 | 65 | 97 | 58 | 95 | 129 | 29 | – | 19 | 11 | 19 | 5 | 9 | 52 | 99 |
Unique Cell Count | 160 | 147 | 22 | 73 | 156 | 178 | 302 | 114 | 180 | 257 | 333 | 93 | 192 | 245 | 36 | 21 | 189 | 166 | 46 | 76 | 241 | 301 | ||
Labelled Cell Count | 226 | 177 | 22 | 84 | 181 | 220 | 354 | 146 | 233 | 371 | 424 | 110 | 227 | 292 | 41 | 28 | 189 | 166 | 46 | 76 | 241 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.6 | 5.3 | 5.8 | 6.2 | 5.4 | 5.5 | – | 4.1 | 4.5 | 4.1 | 4.4 | 6.2 | 6.0 | 5.9 | 6.8 | – | 6.7 | – | 5.5 | 5.8 |
Std Deviation (1e-4) | 0.4 | 1.0 | 0.6 | 1.5 | 1.6 | 1.5 | 2.2 | – | 0.9 | 0.6 | 1.1 | 1.2 | 0.9 | 1.1 | 1.2 | 1.0 | – | 1.6 | – | 1.1 | 1.0 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.23 | 0.02 | 0.04 | – | -0.38 | -0.24 | -0.39 | -0.28 | 0.22 | 0.17 | 0.14 | 0.35 | – | 0.34 | – | 0.06 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleus | 0 | 0 | 2.5 | 2.7 | – | 3.2 | 3.0 | 2.4 | 2.8 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | -1.7 | -0.8 | -2.5 | -3.3 | – | -5.1 | -4.0 | -5.1 | -4.7 | -1.3 | -2.5 | -2.2 | 0.3 | – | 1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3996 | -0.2202 | -0.425 | -0.5693 | -0.2373 | -0.4193 | 0.4115 | 0.9174 | 0.7271 | 0.6962 | -0.0373 | 0.7653 | -0.2674 | -0.0653 | -0.4441 | -0.2823 | -0.3929 | -0.1992 |
Actin | 0.0303 | 0.0002 | 0.0056 | 0.0009 | 0.0096 | 0.0192 | 0.0366 | 0.0001 | 0.0132 | 0.0012 | 0.002 | 0.007 | 0.0755 | 0.0002 | 0.0124 | 0.0397 | 0.0026 | 0.0218 |
Bud | 0.0007 | 0 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0001 |
Bud Neck | 0.0089 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0052 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0008 | 0.0038 | 0.0001 | 0.0003 | 0.0004 | 0.0005 |
Bud Periphery | 0.001 | 0 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0006 | 0 | 0.0011 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0011 | 0.0002 |
Bud Site | 0.0109 | 0 | 0.0007 | 0.0001 | 0.0084 | 0.0002 | 0.0044 | 0 | 0.004 | 0.0001 | 0.0002 | 0.0002 | 0.0019 | 0.0072 | 0.0008 | 0.0013 | 0.0012 | 0.0006 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.0223 | 0.0158 | 0.0268 | 0.0411 | 0.0363 | 0.0184 | 0.0077 | 0.0073 | 0.0054 | 0.0101 | 0.0065 | 0.0055 | 0.0129 | 0.0101 | 0.0131 | 0.0134 | 0.022 | 0.0117 |
Cytoplasmic Foci | 0.0247 | 0.0024 | 0.0079 | 0.0036 | 0.0218 | 0.0014 | 0.0054 | 0.0002 | 0.0064 | 0.0015 | 0.0018 | 0.0044 | 0.0367 | 0.0023 | 0.0081 | 0.0034 | 0.0123 | 0.0128 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.084 | 0.0698 | 0.0566 | 0.0643 | 0.007 | 0.0374 | 0.0257 | 0.0498 | 0.0313 | 0.0638 | 0.0098 | 0.016 | 0.0312 | 0.0548 | 0.0497 | 0.0406 | 0.0309 | 0.0194 |
Endosome | 0.0363 | 0.0158 | 0.0171 | 0.017 | 0.0251 | 0.0063 | 0.0124 | 0.0028 | 0.0127 | 0.0094 | 0.014 | 0.003 | 0.0137 | 0.0035 | 0.007 | 0.0073 | 0.0528 | 0.0103 |
Golgi | 0.0135 | 0.0002 | 0.0056 | 0.0024 | 0.0107 | 0.0006 | 0.0072 | 0 | 0.0051 | 0.0008 | 0.0062 | 0.0006 | 0.009 | 0.0002 | 0.0017 | 0.001 | 0.0178 | 0.0068 |
Lipid Particles | 0.0259 | 0.0066 | 0.0197 | 0.0346 | 0.0242 | 0.0178 | 0.0189 | 0.0003 | 0.0125 | 0.006 | 0.034 | 0.0015 | 0.0396 | 0.0016 | 0.0028 | 0.0029 | 0.0541 | 0.0138 |
Mitochondria | 0.0088 | 0.0009 | 0.0194 | 0.0237 | 0.0136 | 0.0022 | 0.0045 | 0.0008 | 0.0087 | 0.003 | 0.04 | 0.0011 | 0.0051 | 0.0009 | 0.0039 | 0.0024 | 0.0652 | 0.0046 |
None | 0.0347 | 0.0082 | 0.0709 | 0.1521 | 0.0381 | 0.0262 | 0.0123 | 0.0547 | 0.0208 | 0.0427 | 0.0969 | 0.0411 | 0.0363 | 0.0319 | 0.0287 | 0.0364 | 0.0389 | 0.0144 |
Nuclear Periphery | 0.6284 | 0.8141 | 0.6979 | 0.5883 | 0.6683 | 0.7759 | 0.7905 | 0.8118 | 0.814 | 0.8038 | 0.7118 | 0.8654 | 0.6416 | 0.7944 | 0.7923 | 0.795 | 0.5368 | 0.7898 |
Nucleolus | 0.0021 | 0.0002 | 0.0008 | 0.0009 | 0.0011 | 0.0015 | 0.0008 | 0.0003 | 0.0005 | 0.0002 | 0.0017 | 0.0018 | 0.0003 | 0.0128 | 0.0002 | 0.0003 | 0.0013 | 0.0007 |
Nucleus | 0.0197 | 0.0305 | 0.0212 | 0.0174 | 0.0164 | 0.0685 | 0.0347 | 0.0396 | 0.0156 | 0.0157 | 0.0358 | 0.0255 | 0.0232 | 0.0326 | 0.0384 | 0.0202 | 0.1129 | 0.0599 |
Peroxisomes | 0.0087 | 0.0001 | 0.0133 | 0.0123 | 0.0962 | 0.0005 | 0.0076 | 0 | 0.0043 | 0.0003 | 0.0037 | 0.0002 | 0.0461 | 0.0003 | 0.0089 | 0.0017 | 0.0259 | 0.0092 |
Punctate Nuclear | 0.014 | 0.0023 | 0.0031 | 0.0065 | 0.0051 | 0.0055 | 0.0043 | 0.001 | 0.0028 | 0.0008 | 0.0171 | 0.0109 | 0.0078 | 0.007 | 0.0022 | 0.0058 | 0.0027 | 0.0092 |
Vacuole | 0.0048 | 0.0057 | 0.004 | 0.0059 | 0.0021 | 0.0021 | 0.0023 | 0.0022 | 0.0058 | 0.0026 | 0.0026 | 0.0011 | 0.0015 | 0.0037 | 0.0023 | 0.0019 | 0.0069 | 0.0014 |
Vacuole Periphery | 0.0192 | 0.027 | 0.0282 | 0.0284 | 0.0142 | 0.0156 | 0.0181 | 0.0289 | 0.0334 | 0.0375 | 0.0149 | 0.0139 | 0.0156 | 0.0322 | 0.027 | 0.0257 | 0.0129 | 0.0126 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.8932 | 16.8342 | 17.9568 | 23.5271 | 16.7858 | 23.5263 | 26.4623 | 23.335 | 25.7669 | 25.4986 |
Translational Efficiency | 0.3457 | 0.4742 | 0.5146 | 0.4073 | 0.431 | 0.4724 | 0.4709 | 0.4847 | 0.4107 | 0.3644 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1550 | 1200 | 1391 | 1590 | 1621 | 2088 | 298 | 194 | 3171 | 3288 | 1689 | 1784 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 536.97 | 748.16 | 1016.32 | 856.86 | 695.74 | 727.67 | 1035.51 | 958.04 | 618.13 | 735.15 | 1019.71 | 867.86 |
Standard Deviation | 57.96 | 117.82 | 127.48 | 119.60 | 85.95 | 97.84 | 108.74 | 121.41 | 108.25 | 106.03 | 124.60 | 123.87 |
Intensity Change Log 2 | — | 0.478505 | 0.920441 | 0.674218 | — | 0.064736 | 0.573721 | 0.461538 | — | 0.259693 | 0.735078 | 0.558057 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000023 | 0.000119 | 0.000219 | 0.000202 | 0.000042 | 0.000055 | 0.000536 | 0.000682 | 0.000033 | 0.000078 | 0.000275 | 0.000255 |
Bud Neck | 0.002045 | 0.001100 | 0.001794 | 0.005103 | 0.000832 | 0.001463 | 0.001414 | 0.004030 | 0.001425 | 0.001330 | 0.001727 | 0.004986 |
Bud Site | 0.001703 | 0.000526 | 0.000139 | 0.002900 | 0.000210 | 0.000934 | 0.000369 | 0.003352 | 0.000940 | 0.000785 | 0.000180 | 0.002949 |
Cell Periphery | 0.000241 | 0.000082 | 0.000021 | 0.000058 | 0.000074 | 0.000063 | 0.000056 | 0.000053 | 0.000156 | 0.000070 | 0.000027 | 0.000058 |
Cytoplasm | 0.308141* | 0.071276 | 0.006670 | 0.066689 | 0.150460* | 0.075985 | 0.009349 | 0.036009 | 0.227535* | 0.074266 | 0.007143 | 0.063352 |
Cytoplasmic Foci | 0.069130 | 0.024867 | 0.000100 | 0.003253 | 0.031522 | 0.020942 | 0.001470 | 0.003456 | 0.049905 | 0.022374 | 0.000342 | 0.003275 |
Eisosomes | 0.000104 | 0.000029 | 0.000003 | 0.000009 | 0.000029 | 0.000019 | 0.000029 | 0.000011 | 0.000065 | 0.000023 | 0.000008 | 0.000009 |
Endoplasmic Reticulum | 0.043581 | 0.106001* | 0.017017 | 0.034423 | 0.066762 | 0.095937 | 0.043184 | 0.017944 | 0.055431 | 0.099610 | 0.021633 | 0.032631 |
Endosome | 0.003576 | 0.009137 | 0.001030 | 0.008952 | 0.005285 | 0.007495 | 0.003263 | 0.008764 | 0.004450 | 0.008094 | 0.001424 | 0.008931 |
Golgi | 0.000862 | 0.001144 | 0.000024 | 0.001893 | 0.001021 | 0.001977 | 0.000062 | 0.006546 | 0.000943 | 0.001673 | 0.000031 | 0.002399 |
Lipid Particles | 0.123474* | 0.054311 | 0.000257 | 0.001955 | 0.055674 | 0.048116 | 0.002834 | 0.000930 | 0.088815 | 0.050377 | 0.000712 | 0.001843 |
Mitochondria | 0.008333 | 0.012863 | 0.001903 | 0.003914 | 0.007151 | 0.015111 | 0.002004 | 0.001942 | 0.007729 | 0.014291 | 0.001921 | 0.003700 |
Mitotic Spindle | 0.000108 | 0.003563 | 0.001477 | 0.026684 | 0.001307 | 0.005114 | 0.001440 | 0.036774 | 0.000721 | 0.004548 | 0.001470 | 0.027782 |
None | 0.021753 | 0.007109 | 0.000388 | 0.003938 | 0.006366 | 0.003850 | 0.000601 | 0.002112 | 0.013887 | 0.005039 | 0.000426 | 0.003739 |
Nuclear Periphery | 0.112721* | 0.489507* | 0.788348* | 0.577589* | 0.446481* | 0.543917* | 0.581006* | 0.631884* | 0.283337* | 0.524059* | 0.751765* | 0.583493* |
Nuclear Periphery Foci | 0.017538 | 0.091570 | 0.053265 | 0.126490 | 0.059260 | 0.072751 | 0.100480 | 0.149896 | 0.038866 | 0.079619 | 0.061595 | 0.129036 |
Nucleolus | 0.004261 | 0.010317 | 0.010929 | 0.007531 | 0.005862 | 0.004639 | 0.021181 | 0.006733 | 0.005079 | 0.006711 | 0.012738 | 0.007444 |
Nucleus | 0.271158* | 0.088499 | 0.108819 | 0.088808 | 0.146559* | 0.076058 | 0.205517* | 0.047972 | 0.207464* | 0.080599 | 0.125880 | 0.084367 |
Peroxisomes | 0.002151 | 0.001929 | 0.000132 | 0.000202 | 0.001151 | 0.001613 | 0.000628 | 0.000352 | 0.001640 | 0.001728 | 0.000220 | 0.000218 |
Vacuole | 0.008097 | 0.020492 | 0.005455 | 0.033790 | 0.011668 | 0.018404 | 0.023193 | 0.034811 | 0.009922 | 0.019166 | 0.008585 | 0.033901 |
Vacuole Periphery | 0.001000 | 0.005559 | 0.002011 | 0.005618 | 0.002284 | 0.005558 | 0.001386 | 0.005745 | 0.001657 | 0.005558 | 0.001900 | 0.005632 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.24 | -10.36 | -8.98 | -4.57 | -0.08 | -1.96 | -7.99 | -1.52 | -1.48 | -0.08 | -6.47 | -12.68 | -5.28 | -4.34 | 0.37 |
Bud Neck | 2.74 | -0.44 | -7.07 | -8.60 | -6.68 | -2.24 | -2.01 | -4.44 | -3.95 | -3.70 | 0.28 | -2.23 | -8.91 | -9.00 | -7.45 |
Bud Site | 2.05 | 2.73 | -2.18 | -4.70 | -5.35 | -2.22 | -2.02 | -1.93 | -1.45 | -1.73 | 0.38 | 2.51 | -4.00 | -4.35 | -5.59 |
Cell Periphery | 7.50 | 10.54 | 8.23 | 2.28 | -6.24 | 1.90 | 0.19 | 0.89 | 0.16 | 0.65 | 7.87 | 11.59 | 7.76 | 0.93 | -5.03 |
Cytoplasm | 31.31 | 47.72 | 30.23 | -1.41 | -17.32 | 12.07 | 24.12 | 9.55 | 2.77 | -3.89 | 30.53 | 50.07 | 25.98 | -0.51 | -17.61 |
Cytoplasmic Foci | 12.65 | 26.35 | 24.61 | 9.86 | -6.86 | 4.55 | 14.95 | 12.16 | 9.23 | -0.67 | 13.33 | 30.06 | 27.24 | 15.17 | -6.30 |
Eisosomes | 8.15 | 11.31 | 10.21 | 7.40 | -8.56 | 5.50 | -1.72 | 2.73 | 0.20 | 3.24 | 9.41 | 12.29 | 11.51 | 6.61 | -2.75 |
Endoplasmic Reticulum | -12.10 | 9.11 | 3.30 | 15.50 | -8.18 | -7.32 | 5.89 | 14.00 | 19.60 | 6.71 | -14.01 | 16.11 | 10.00 | 22.82 | -5.20 |
Endosome | -5.42 | 10.13 | -5.48 | 0.11 | -8.10 | -2.79 | 2.04 | -1.81 | -0.73 | -2.45 | -6.01 | 11.16 | -4.93 | -0.86 | -8.21 |
Golgi | -1.49 | 8.15 | -1.46 | -0.84 | -3.03 | -1.72 | 3.46 | -1.34 | -1.02 | -1.65 | -2.14 | 6.09 | -1.81 | -0.53 | -3.42 |
Lipid Particles | 13.32 | 29.52 | 29.05 | 17.12 | -9.86 | 2.17 | 20.83 | 21.85 | 20.18 | 4.34 | 12.34 | 35.55 | 35.00 | 26.17 | -6.56 |
Mitochondria | -3.02 | 8.61 | 4.62 | 6.07 | -3.55 | -5.70 | 6.43 | 5.73 | 10.61 | -0.69 | -6.39 | 10.45 | 5.22 | 10.23 | -3.61 |
Mitotic Spindle | -3.36 | -3.47 | -11.09 | -9.40 | -10.36 | -3.77 | -1.00 | -4.38 | -3.91 | -3.95 | -5.22 | -2.32 | -11.72 | -10.03 | -10.99 |
None | 6.73 | 11.25 | 8.40 | 2.17 | -4.64 | 2.91 | 6.94 | 3.04 | 1.33 | -1.70 | 7.94 | 13.10 | 7.67 | 1.09 | -4.81 |
Nuclear Periphery | -33.21* | -72.79* | -38.64* | -2.48 | 21.57 | -8.43 | -4.57 | -3.15 | 0.43 | 0.44 | -28.17 | -47.50 | -22.76 | 0.05 | 18.72 |
Nuclear Periphery Foci | -18.42* | -14.06 | -22.00 | -4.89 | -13.28 | -3.48 | -4.66 | -5.68 | -4.72 | -2.47 | -15.00 | -7.91 | -18.28 | -8.84 | -12.42 |
Nucleolus | -4.81 | -6.63 | -5.00 | -0.01 | 0.49 | 1.86 | -4.07 | -1.45 | -1.84 | 1.82 | -2.39 | -7.13 | -4.58 | -3.10 | 1.55 |
Nucleus | 22.42 | 17.59 | 19.88 | -3.41 | 0.57 | 11.74 | -4.59 | 3.65 | -1.64 | 6.21 | 25.19 | 10.00 | 15.75 | -5.73 | 3.42 |
Peroxisomes | 0.36 | 8.99 | 8.44 | 4.67 | -2.87 | -1.42 | 2.29 | 2.95 | 4.28 | 1.60 | -0.50 | 8.97 | 8.63 | 7.11 | -0.76 |
Vacuole | -7.51 | 0.97 | -13.12 | -8.03 | -13.05 | -5.07 | -4.44 | -4.75 | -4.21 | -2.89 | -9.05 | -1.31 | -13.38 | -9.93 | -12.06 |
Vacuole Periphery | -10.46 | -2.63 | -5.87 | 0.50 | -3.91 | -6.28 | 2.82 | -1.68 | 0.45 | -2.27 | -10.82 | -0.75 | -5.27 | 0.62 | -4.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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