Standard name
Human Ortholog
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.06 0.07 0.14 0.21 0.06 0.15 0.13 0.06 0.13 0.11 0.14 0.13 0.22 0.26 0.11 0.05 0 0 0.06 0.12 0.11 0.11
Endoplasmic Reticulum 0 0 0 0.15 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0.17 0 0 0
Endosome 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 0 0 0 0.08 0.15 0.35 0.23 0.61 0.38 0 0 0 0 0.14 0.08 0.07 0 0.13 0.13 0.13
Nucleus 0 0.1 0.05 0.16 0.19 0.29 0.32 0.39 0.36 0.28 0.34 0.14 0.14 0.17 0 0 0 0 0 0 0.1 0.08
Nuclear Periphery 0.29 0.14 0 0 0.14 0.19 0.12 0.21 0.18 0.15 0.1 0.24 0.26 0.18 0.11 0.14 0.72 0.72 0.55 0.44 0.26 0.21
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.63 0.82 0.77 0.58 0.69 0.49 0.41 0.22 0.33 0.25 0.29 0.62 0.49 0.53 0.81 0.9 0.06 0.12 0.11 0.13 0.22 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 1 0 3 2 0 0 0 0 0 0 0 0 0 4 2
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 2 3 2 0 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 9 11 3 15 9 27 39 7 23 27 45 12 43 63 4 1 3 2 2 8 27 33
Endoplasmic Reticulum 7 5 0 11 0 0 0 3 4 5 1 5 5 5 1 0 9 5 7 2 10 12
Endosome 1 1 1 0 5 4 11 0 3 0 4 0 2 4 1 1 3 0 0 0 7 8
Golgi 1 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 1 1 0 0 2 0
Mitochondria 55 4 0 3 6 15 44 40 41 157 125 0 0 1 1 3 14 10 1 10 31 39
Nucleus 6 14 1 12 29 52 98 45 65 73 112 13 27 41 1 0 1 3 0 3 24 25
Nuclear Periphery 46 21 0 1 22 34 36 24 32 38 33 22 50 45 4 3 135 119 25 33 62 64
Nucleolus 0 0 0 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 1 0 3 0 3 2 0 0 4 1 0 1 9 5
Vac/Vac Membrane 101 121 17 42 108 88 124 25 60 65 97 58 95 129 29 19 11 19 5 9 52 99
Unique Cell Count 160 147 22 73 156 178 302 114 180 257 333 93 192 245 36 21 189 166 46 76 241 301
Labelled Cell Count 226 177 22 84 181 220 354 146 233 371 424 110 227 292 41 28 189 166 46 76 241 301


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.6 5.3 5.8 6.2 5.4 5.5 4.1 4.5 4.1 4.4 6.2 6.0 5.9 6.8 6.7 5.5 5.8
Std Deviation (1e-4) 0.4 1.0 0.6 1.5 1.6 1.5 2.2 0.9 0.6 1.1 1.2 0.9 1.1 1.2 1.0 1.6 1.1 1.0
Intensity Change (Log2) 0.12 0.23 0.02 0.04 -0.38 -0.24 -0.39 -0.28 0.22 0.17 0.14 0.35 0.34 0.06 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 1.3 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 2.5 2.7 3.2 3.0 2.4 2.8 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2.7 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 -0.8 -2.5 -3.3 -5.1 -4.0 -5.1 -4.7 -1.3 -2.5 -2.2 0.3 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3996 -0.2202 -0.425 -0.5693 -0.2373 -0.4193 0.4115 0.9174 0.7271 0.6962 -0.0373 0.7653 -0.2674 -0.0653 -0.4441 -0.2823 -0.3929 -0.1992
Actin 0.0303 0.0002 0.0056 0.0009 0.0096 0.0192 0.0366 0.0001 0.0132 0.0012 0.002 0.007 0.0755 0.0002 0.0124 0.0397 0.0026 0.0218
Bud 0.0007 0 0.0003 0 0.0005 0.0001 0.0003 0 0.0007 0 0.0001 0 0.0002 0.0002 0.0002 0.0002 0.0007 0.0001
Bud Neck 0.0089 0 0.0002 0.0001 0.0004 0.0003 0.0052 0 0.0004 0.0001 0.0004 0.0004 0.0008 0.0038 0.0001 0.0003 0.0004 0.0005
Bud Periphery 0.001 0 0.0002 0.0001 0.0007 0.0001 0.0006 0 0.0011 0.0001 0.0003 0.0001 0.0003 0.0002 0.0002 0.0003 0.0011 0.0002
Bud Site 0.0109 0 0.0007 0.0001 0.0084 0.0002 0.0044 0 0.004 0.0001 0.0002 0.0002 0.0019 0.0072 0.0008 0.0013 0.0012 0.0006
Cell Periphery 0.0005 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0005 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0.0003 0.0001
Cytoplasm 0.0223 0.0158 0.0268 0.0411 0.0363 0.0184 0.0077 0.0073 0.0054 0.0101 0.0065 0.0055 0.0129 0.0101 0.0131 0.0134 0.022 0.0117
Cytoplasmic Foci 0.0247 0.0024 0.0079 0.0036 0.0218 0.0014 0.0054 0.0002 0.0064 0.0015 0.0018 0.0044 0.0367 0.0023 0.0081 0.0034 0.0123 0.0128
Eisosomes 0.0006 0 0.0002 0.0001 0.0002 0.0001 0.0003 0 0.0006 0.0002 0.0001 0 0.0006 0 0.0001 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.084 0.0698 0.0566 0.0643 0.007 0.0374 0.0257 0.0498 0.0313 0.0638 0.0098 0.016 0.0312 0.0548 0.0497 0.0406 0.0309 0.0194
Endosome 0.0363 0.0158 0.0171 0.017 0.0251 0.0063 0.0124 0.0028 0.0127 0.0094 0.014 0.003 0.0137 0.0035 0.007 0.0073 0.0528 0.0103
Golgi 0.0135 0.0002 0.0056 0.0024 0.0107 0.0006 0.0072 0 0.0051 0.0008 0.0062 0.0006 0.009 0.0002 0.0017 0.001 0.0178 0.0068
Lipid Particles 0.0259 0.0066 0.0197 0.0346 0.0242 0.0178 0.0189 0.0003 0.0125 0.006 0.034 0.0015 0.0396 0.0016 0.0028 0.0029 0.0541 0.0138
Mitochondria 0.0088 0.0009 0.0194 0.0237 0.0136 0.0022 0.0045 0.0008 0.0087 0.003 0.04 0.0011 0.0051 0.0009 0.0039 0.0024 0.0652 0.0046
None 0.0347 0.0082 0.0709 0.1521 0.0381 0.0262 0.0123 0.0547 0.0208 0.0427 0.0969 0.0411 0.0363 0.0319 0.0287 0.0364 0.0389 0.0144
Nuclear Periphery 0.6284 0.8141 0.6979 0.5883 0.6683 0.7759 0.7905 0.8118 0.814 0.8038 0.7118 0.8654 0.6416 0.7944 0.7923 0.795 0.5368 0.7898
Nucleolus 0.0021 0.0002 0.0008 0.0009 0.0011 0.0015 0.0008 0.0003 0.0005 0.0002 0.0017 0.0018 0.0003 0.0128 0.0002 0.0003 0.0013 0.0007
Nucleus 0.0197 0.0305 0.0212 0.0174 0.0164 0.0685 0.0347 0.0396 0.0156 0.0157 0.0358 0.0255 0.0232 0.0326 0.0384 0.0202 0.1129 0.0599
Peroxisomes 0.0087 0.0001 0.0133 0.0123 0.0962 0.0005 0.0076 0 0.0043 0.0003 0.0037 0.0002 0.0461 0.0003 0.0089 0.0017 0.0259 0.0092
Punctate Nuclear 0.014 0.0023 0.0031 0.0065 0.0051 0.0055 0.0043 0.001 0.0028 0.0008 0.0171 0.0109 0.0078 0.007 0.0022 0.0058 0.0027 0.0092
Vacuole 0.0048 0.0057 0.004 0.0059 0.0021 0.0021 0.0023 0.0022 0.0058 0.0026 0.0026 0.0011 0.0015 0.0037 0.0023 0.0019 0.0069 0.0014
Vacuole Periphery 0.0192 0.027 0.0282 0.0284 0.0142 0.0156 0.0181 0.0289 0.0334 0.0375 0.0149 0.0139 0.0156 0.0322 0.027 0.0257 0.0129 0.0126

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.8932 16.8342 17.9568 23.5271 16.7858 23.5263 26.4623 23.335 25.7669 25.4986
Translational Efficiency 0.3457 0.4742 0.5146 0.4073 0.431 0.4724 0.4709 0.4847 0.4107 0.3644

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
Localization
Cell Percentages nuclear periphery (23%), ER (11%), mixed (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Asi1

Asi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available