Standard name
Human Ortholog
Description Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 1.0 0.97 0.93 0.94 – 0.85 0.79 0.71 0.74 0.99 1.0 1.0 0.96 0.98 0.97 0.95 0.92 0.95 0.94 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.06 – 0.25 0.29 0.44 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 1 0 0 1 0 1
Bud 0 0 0 0 0 0 1 – 2 3 7 6 0 1 1 1 0 0 0 0 0 2 1 8
Bud Neck 0 0 1 0 1 0 1 – 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 1 3 – 4 4 3 1 0 0 2 4 10 8 0 0 0 0 0 0
Cytoplasm 287 148 97 216 342 266 426 – 258 334 258 272 170 391 459 298 200 259 280 145 105 229 277 333
Endoplasmic Reticulum 1 0 0 0 0 1 0 – 0 0 1 1 1 0 1 17 11 15 0 0 0 1 4 1
Endosome 0 0 0 0 0 0 0 – 2 2 1 2 0 0 0 2 0 1 4 1 1 1 1 6
Golgi 0 1 0 0 1 0 0 – 0 0 0 0 0 0 2 1 0 1 3 3 0 0 2 2
Mitochondria 1 4 0 2 4 39 25 – 75 122 158 144 1 4 1 4 2 2 2 1 1 1 5 6
Nucleus 0 0 3 0 0 3 2 – 3 4 4 6 0 0 0 1 0 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 1 2 0 1 0 0 0 2 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 3 – 3 3 1 2 0 0 0 0 0 0 1 0 0 1 1 4
Vac/Vac Membrane 8 0 0 0 10 4 19 – 12 17 15 8 1 0 1 5 3 5 1 1 0 1 1 4
Unique Cell Count 297 151 99 217 352 286 452 305 422 361 369 172 392 460 309 205 267 297 157 111 244 301 377
Labelled Cell Count 298 153 102 219 360 315 481 360 493 453 443 173 396 467 336 227 293 297 157 111 244 301 377


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.3 6.1 5.4 6.2 4.7 5.1 – 4.5 4.3 4.0 4.1 5.5 4.8 5.7 5.9 6.5 6.4 5.6 5.3 5.4
Std Deviation (1e-4) 0.7 0.8 2.7 1.2 1.7 2.8 1.5 – 1.7 1.0 0.9 0.9 1.5 1.3 1.6 1.0 1.4 1.4 3.7 1.7 1.8
Intensity Change (Log2) – – – -0.18 0.02 -0.39 -0.27 – -0.44 -0.5 -0.6 -0.57 -0.15 -0.35 -0.1 -0.05 0.08 0.07 -0.12 -0.2 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 -0.4 -1.8 -1.5 – -3.5 -4.5 -5.6 -5.2 0.6 2.0 2.2 -0.8 -0.2 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2519 -0.7546 -0.8876 -1.274 -1.1054 -0.8845 0.2282 0.5696 0.46 0.2869 0.1525 0.422 1.4616 2.3627 2.1956 1.497 1.8684 2.062
Actin 0.0017 0.0006 0.0016 0.0026 0.0009 0.0006 0.0148 0.0088 0.002 0.0001 0.0023 0.0003 0.0279 0.0001 0.0219 0.0174 0.0493 0.0069
Bud 0.0011 0.0006 0.0001 0.0003 0 0.0001 0.0008 0.0021 0.0023 0.0001 0.0001 0.0001 0.0008 0 0.0008 0.0001 0.0009 0.0012
Bud Neck 0.0078 0.0001 0.0001 0.0004 0.0002 0.0003 0.0029 0.0004 0.0001 0.0001 0.0001 0.0002 0.0007 0 0.0009 0.0001 0.0009 0.0002
Bud Periphery 0.0016 0.0004 0.0002 0.0008 0.0001 0.0001 0.0013 0.0054 0.0072 0.0001 0.0002 0.0002 0.0014 0 0.0016 0.0001 0.002 0.0033
Bud Site 0.001 0.0014 0.0004 0.0003 0 0.0001 0.0182 0.0092 0.0013 0 0.0003 0 0.0104 0.0001 0.0063 0.0004 0.0021 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.002 0.0004 0.0002 0 0 0.0001 0.0006 0.0001 0.0003 0 0.0005 0.0001
Cytoplasm 0.1488 0.2176 0.1937 0.2058 0.2212 0.2592 0.2425 0.3395 0.2921 0.2902 0.4672 0.4706 0.1213 0.2732 0.1866 0.1876 0.285 0.2993
Cytoplasmic Foci 0.0232 0.0066 0.0093 0.0182 0.012 0.018 0.0192 0.0053 0.0061 0.0042 0.0121 0.0029 0.0161 0.0022 0.011 0.0071 0.0051 0.0087
Eisosomes 0.0003 0.0002 0.0009 0.0003 0.0001 0 0.0003 0.0001 0.0001 0 0 0 0.0005 0.0001 0.0005 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.002 0.0012 0.0016 0.0045 0.0008 0.0007 0.0031 0.0036 0.0021 0.0007 0.0012 0.0009 0.0066 0.0015 0.0024 0.0041 0.0022 0.0024
Endosome 0.0069 0.0011 0.004 0.0058 0.0019 0.0024 0.0104 0.0028 0.0022 0.001 0.0294 0.0009 0.0161 0.0005 0.0082 0.0025 0.0208 0.0143
Golgi 0.001 0.0001 0.0003 0.0008 0.0001 0.0002 0.0055 0.0028 0.0006 0 0.0136 0 0.0047 0.0001 0.005 0.0013 0.0053 0.0053
Lipid Particles 0.0051 0.0003 0.0006 0.0044 0.0002 0.0009 0.0092 0.001 0.0009 0 0.0093 0 0.0064 0.0001 0.0063 0.0013 0.0045 0.0063
Mitochondria 0.0008 0.0003 0.0004 0.0264 0.0002 0.0003 0.0071 0.0022 0.0008 0.0001 0.0073 0.0001 0.0043 0.0001 0.019 0.0004 0.0149 0.0013
None 0.782 0.7635 0.782 0.7146 0.7598 0.709 0.6351 0.608 0.6713 0.7016 0.4482 0.5219 0.7505 0.7207 0.7066 0.7546 0.5542 0.6338
Nuclear Periphery 0.0038 0.0007 0.0009 0.0039 0.0005 0.0004 0.0058 0.0019 0.0037 0.0003 0.003 0.0002 0.0058 0.0003 0.0074 0.0008 0.021 0.0057
Nucleolus 0.0004 0.0002 0.0002 0.0005 0.0001 0.0001 0.0004 0.0003 0.0002 0 0.0001 0 0.0005 0 0.0005 0 0.001 0.0001
Nucleus 0.0015 0.0024 0.0011 0.0023 0.0006 0.0014 0.0017 0.0023 0.0032 0.0006 0.0008 0.001 0.0025 0.0004 0.0038 0.0008 0.0099 0.0011
Peroxisomes 0.0041 0.0002 0.0003 0.0024 0.0001 0.0025 0.0138 0.0012 0.0013 0.0001 0.0014 0 0.0121 0 0.0072 0.02 0.0022 0.0074
Punctate Nuclear 0.0032 0.0016 0.0011 0.0038 0.0008 0.0031 0.0037 0.0006 0.0013 0.0004 0.001 0.0002 0.0049 0.0002 0.0018 0.0005 0.0027 0.0015
Vacuole 0.0031 0.0006 0.0008 0.0012 0.0004 0.0004 0.0016 0.0017 0.0007 0.0002 0.001 0.0003 0.0046 0.0003 0.0012 0.0007 0.0105 0.0005
Vacuole Periphery 0.0006 0.0001 0.0001 0.0007 0.0001 0.0001 0.0007 0.0003 0.0002 0 0.0013 0 0.0012 0 0.0007 0.0001 0.0048 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.5196 17.3048 1.1674 4.4269 2.2817 10.553 4.7719 6.4414 6.7247 7.2042
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
224 1045 896 1566 893 2045 186 1433 1117 3090 1082 2999

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.54 745.52 942.73 970.14 676.54 773.91 994.82 902.49 670.52 764.31 951.68 937.82
Standard Deviation 75.28 124.83 109.05 859.07 113.35 93.10 106.66 120.49 107.48 105.77 110.41 627.25
Intensity Change Log 2 — 0.205507 0.544105 0.585453 — 0.193991 0.556260 0.415736 — 0.199630 0.550333 0.501166

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000263 0.000254 0.000348 0.000734 0.000077 0.000188 0.000477 0.000456 0.000115 0.000210 0.000371 0.000601
Bud Neck 0.032933 0.013458 0.003064 0.002503 0.007214 0.020350 0.001922 0.005793 0.012372 0.018019 0.002868 0.004075
Bud Site 0.010208 0.013055 0.010081 0.028415 0.002930 0.017540 0.004243 0.023297 0.004390 0.016023 0.009077 0.025970
Cell Periphery 0.000636 0.000123 0.000177 0.000469 0.000198 0.000102 0.000250 0.000105 0.000286 0.000109 0.000190 0.000295
Cytoplasm 0.370026* 0.570036* 0.477163* 0.645590* 0.529701* 0.564910* 0.415938* 0.634838* 0.497680* 0.566644* 0.466638* 0.640452*
Cytoplasmic Foci 0.359419* 0.179410 0.015908 0.031547 0.217547* 0.145365 0.020356 0.024881 0.245998* 0.156879 0.016673 0.028362
Eisosomes 0.000588 0.000071 0.000048 0.000041 0.000206 0.000078 0.000090 0.000029 0.000282 0.000075 0.000056 0.000035
Endoplasmic Reticulum 0.001830 0.000773 0.004092 0.000974 0.001218 0.000535 0.005660 0.001194 0.001340 0.000615 0.004362 0.001079
Endosome 0.017975 0.020551 0.003891 0.003037 0.012035 0.012109 0.006037 0.004568 0.013226 0.014964 0.004260 0.003769
Golgi 0.009162 0.003970 0.000375 0.000970 0.001543 0.002640 0.000130 0.003304 0.003071 0.003090 0.000333 0.002085
Lipid Particles 0.017703 0.003064 0.002757 0.000717 0.007789 0.002831 0.003183 0.000771 0.009777 0.002910 0.002830 0.000743
Mitochondria 0.021250 0.004012 0.000547 0.000258 0.001962 0.005398 0.000241 0.000547 0.005830 0.004929 0.000494 0.000396
Mitotic Spindle 0.003587 0.002713 0.002587 0.010952 0.001316 0.009194 0.000832 0.034925 0.001771 0.007002 0.002285 0.022407
None 0.006165 0.009183 0.004480 0.011286 0.009404 0.004344 0.004557 0.004698 0.008755 0.005981 0.004493 0.008138
Nuclear Periphery 0.000296 0.000252 0.001794 0.000387 0.000513 0.000454 0.001650 0.000679 0.000469 0.000385 0.001769 0.000527
Nuclear Periphery Foci 0.000316 0.000805 0.002763 0.001489 0.000711 0.000251 0.007279 0.001731 0.000632 0.000438 0.003540 0.001604
Nucleolus 0.001738 0.001559 0.000451 0.000170 0.001770 0.001151 0.000391 0.000187 0.001764 0.001289 0.000440 0.000178
Nucleus 0.091422 0.087688 0.369315* 0.105599 0.148917* 0.128648 0.275830* 0.139782* 0.137387* 0.114796 0.353245* 0.121932
Peroxisomes 0.013128 0.004970 0.000308 0.000550 0.002299 0.005827 0.000296 0.001696 0.004471 0.005537 0.000306 0.001098
Vacuole 0.036357 0.080912 0.099543 0.153992 0.051773 0.076816 0.250458* 0.115103 0.048682 0.078201 0.125486 0.135410
Vacuole Periphery 0.004999 0.003142 0.000307 0.000322 0.000877 0.001270 0.000182 0.001417 0.001703 0.001903 0.000285 0.000845

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.04 -3.23 -1.16 -1.19 -0.33 -5.41 -10.99 -5.96 -3.53 3.35 -4.78 -11.74 -2.91 -2.04 0.21
Bud Neck 3.46 5.30 5.47 8.70 1.99 -8.82 4.74 2.82 11.38 -1.73 -3.55 6.55 6.50 14.63 0.22
Bud Site -1.08 0.47 -2.25 -1.76 -2.96 -9.11 -4.06 -6.38 1.54 -4.11 -9.40 -2.49 -7.61 0.15 -3.50
Cell Periphery 4.43 3.90 2.20 -1.01 -0.54 2.68 -0.84 2.95 1.38 6.52 4.73 2.45 1.43 -1.00 0.24
Cytoplasm -11.04 -1.27 -10.27 1.85 -13.08 -3.23 10.73 1.41 5.10 -10.09 -7.24 10.40 -3.47 4.71 -15.37
Cytoplasmic Foci 11.58 23.92 22.94 26.44 -8.45 9.59 30.15 30.51 32.10 -1.22 12.84 37.58 36.10 41.09 -7.32
Eisosomes 7.44 7.60 7.84 6.50 4.90 2.12 1.63 2.76 9.16 9.37 4.11 4.24 4.78 10.81 8.95
Endoplasmic Reticulum 2.12 -6.84 1.05 -5.76 14.70 2.50 -10.10 -1.80 -9.07 10.09 3.04 -13.44 -0.71 -10.69 16.79
Endosome -0.73 4.38 4.94 12.14 5.12 0.06 4.73 8.02 11.55 5.58 -1.21 7.80 9.58 16.90 6.65
Golgi 3.79 7.35 7.11 4.54 -0.93 -2.19 7.07 0.85 2.33 -2.08 0.08 8.85 5.89 4.95 -2.04
Lipid Particles 5.86 5.97 6.72 7.64 2.90 5.49 5.02 7.87 5.39 8.13 7.82 7.42 10.28 8.09 3.66
Mitochondria 4.68 5.68 5.80 4.72 2.61 -3.41 2.77 2.49 6.52 -3.93 0.95 5.74 6.07 8.05 1.89
Mitotic Spindle 0.30 0.30 -1.44 -3.03 -3.37 -4.58 -0.35 -7.44 -4.51 -7.58 -3.78 -0.74 -7.50 -4.56 -7.90
None -0.71 0.64 -0.09 1.15 -1.96 2.57 3.54 2.65 -0.29 -3.23 1.60 3.11 1.82 0.30 -2.52
Nuclear Periphery 0.71 -22.57 -8.17 -13.90 18.85 0.33 -10.30 -9.30 -5.42 5.21 0.65 -20.67 -8.86 -6.01 16.36
Nuclear Periphery Foci -4.32 -12.29 -9.36 -6.38 4.93 5.43 -8.37 -6.65 -8.91 6.37 2.41 -13.63 -9.93 -11.96 7.06
Nucleolus 0.26 1.86 2.22 7.44 5.25 1.80 6.15 6.23 4.15 -0.01 1.57 5.78 6.54 7.06 3.72
Nucleus 0.33 -24.52 -3.96 -7.88 27.57 3.08 -7.14 -3.56 -8.01 5.76 4.04 -25.27 -2.35 -9.04 26.15
Peroxisomes 3.81 6.34 6.29 9.09 -1.13 -5.49 8.03 3.91 7.55 -1.50 -1.88 8.90 7.94 10.91 -1.75
Vacuole -8.99 -15.33 -28.70 -22.72 -11.32 -6.73 -14.50 -24.81 -21.31 5.35 -9.81 -19.11 -37.29 -31.72 -7.26
Vacuole Periphery 1.47 3.96 4.01 5.04 0.79 -2.00 5.13 1.51 3.10 -1.69 -0.47 5.39 4.76 6.49 -0.91
Standard name
Human Ortholog
Description Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
Localization
Cell Percentages cytoplasm (52%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Jlp2

Jlp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jlp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available