Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in telomeric silencing; required for quiescent cell telomere hypercluster localization at nuclear membrane vicinity; interacts with PAD4-domain of Sir4p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 |
Bud | 0 | 1 | 3 | 1 | 1 | 5 | 7 | 4 | 3 | 9 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 2 | 3 | 4 | 9 | 14 | 2 | 9 | 4 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 9 | 9 | 8 | 10 | 5 | 7 | 11 | 9 | 5 | 7 | 5 | 19 | 34 | 34 | 17 | 36 | 41 | 0 | 1 | 0 | 1 | 5 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 15 | 12 | 0 | 1 | 1 | 1 | 1 | 0 |
Endosome | 36 | 8 | 7 | 2 | 19 | 2 | 9 | 2 | 4 | 1 | 2 | 1 | 28 | 12 | 3 | 19 | 42 | 58 | 21 | 35 | 41 | 21 | 37 | 43 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 2 | 0 | 3 | 2 | 6 |
Mitochondria | 27 | 100 | 187 | 164 | 151 | 233 | 249 | 294 | 236 | 258 | 165 | 175 | 29 | 62 | 113 | 12 | 22 | 43 | 6 | 12 | 15 | 12 | 8 | 21 |
Nucleus | 10 | 14 | 11 | 21 | 37 | 39 | 53 | 73 | 20 | 46 | 24 | 26 | 6 | 18 | 17 | 6 | 10 | 18 | 2 | 10 | 5 | 7 | 6 | 2 |
Nuclear Periphery | 80 | 110 | 78 | 85 | 116 | 137 | 164 | 125 | 131 | 90 | 73 | 85 | 105 | 87 | 100 | 61 | 140 | 135 | 148 | 116 | 172 | 58 | 110 | 104 |
Nucleolus | 36 | 41 | 34 | 34 | 32 | 62 | 79 | 61 | 76 | 62 | 48 | 43 | 47 | 26 | 35 | 30 | 51 | 65 | 5 | 19 | 7 | 6 | 7 | 7 |
Peroxisomes | 1 | 0 | 6 | 0 | 1 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 1 | 6 | 6 | 0 | 3 | 1 | 2 | 1 | 6 | 1 | 4 | 10 |
SpindlePole | 3 | 6 | 5 | 4 | 4 | 3 | 6 | 6 | 3 | 2 | 4 | 0 | 3 | 10 | 15 | 2 | 7 | 12 | 8 | 10 | 11 | 6 | 13 | 13 |
Vac/Vac Membrane | 29 | 35 | 21 | 10 | 23 | 24 | 25 | 10 | 15 | 11 | 6 | 13 | 34 | 31 | 24 | 31 | 89 | 85 | 1 | 8 | 7 | 6 | 6 | 7 |
Unique Cell Count | 149 | 207 | 257 | 208 | 280 | 346 | 413 | 420 | 319 | 330 | 226 | 213 | 173 | 209 | 240 | 121 | 291 | 327 | 205 | 227 | 281 | 132 | 220 | 230 |
Labelled Cell Count | 225 | 325 | 362 | 331 | 397 | 515 | 613 | 603 | 501 | 494 | 339 | 357 | 272 | 286 | 352 | 186 | 419 | 472 | 205 | 227 | 281 | 132 | 220 | 230 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 5.3 | 4.7 | 4.6 | 5.5 | 4.9 | 4.9 | 4.7 | 4.3 | 4.2 | 4.3 | 4.1 | 6.5 | 5.9 | 5.3 | 7.8 | 8.8 | 8.7 | 6.3 | 6.4 | 6.9 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.0 | 1.1 | 1.2 | 0.9 | 1.1 | 1.0 | 1.2 | 1.0 | 1.2 | 1.2 | 1.7 | 1.6 | 1.4 | 1.5 | 1.9 | 2.1 | 1.5 | 1.9 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.21 | 0.07 | 0.07 | -0.02 | -0.13 | -0.17 | -0.12 | -0.2 | 0.46 | 0.33 | 0.16 | 0.72 | 0.9 | 0.88 | 0.43 | 0.44 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | 0 | -1.7 | -1.5 | -0.7 | -0.5 | -1.6 | -0.2 | -0.7 | 3.1 | 4.7 | 4.2 | 3.8 | 3.8 | 3.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.1 |
Endosome | 0 | 2.2 | 0 | -0.4 | 0 | 0 | 0 | 0 | 0 | 5.0 | 1.6 | 0 | 4.7 | 4.8 | 5.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -4.5 | -1.4 | -3.3 | 0 | 0 | 0 | 0 | 0 | -11.4 | -9.3 | -5.8 | -11.4 | -15.7 | -14.6 |
Nucleus | 2.5 | 3.6 | 3.1 | 3.7 | 5.0 | 1.1 | 3.9 | 2.7 | 3.2 | -0.4 | 1.9 | 1.4 | 0.3 | -0.5 | 0.7 |
Nuclear Periphery | 0 | 2.7 | 2.3 | 2.5 | 0 | 0 | 0 | 0 | 0 | 6.2 | 2.5 | 2.6 | 3.8 | 4.2 | 2.7 |
Nucleolus | 0 | -0.6 | 1.6 | 2.0 | 0 | 0 | 0 | 0 | 0 | 3.6 | -0.3 | 0.4 | 2.8 | 1.4 | 2.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 0.1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 2.4 | 0 | 0.4 | 1.2 |
Vacuole | -1.4 | 0 | -0.6 | -1.1 | -3.5 | -1.7 | -2.6 | -2.6 | -0.9 | 3.5 | 2.3 | 0.7 | 4.6 | 6.5 | 5.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0758 | 3.2953 | 3.1536 | 3.0316 | 2.6398 | 3.1275 | 2.6814 | 3.6036 | 3.4366 | 2.6215 | 2.2004 | 3.0699 | 3.7309 | 4.1897 | 4.5399 | 4.0435 | 3.5163 | 3.9237 |
Actin | 0.0473 | 0.0018 | 0.0101 | 0.013 | 0.0793 | 0.0096 | 0.0401 | 0.0031 | 0.0059 | 0.0058 | 0.0014 | 0.0086 | 0.0121 | 0.0074 | 0.007 | 0.0096 | 0.0006 | 0.0013 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0009 | 0.0013 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0 | 0 |
Bud Neck | 0.0019 | 0.0008 | 0.0018 | 0.0271 | 0.0066 | 0.0013 | 0.0006 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0007 | 0.0009 | 0.0004 | 0.0055 | 0.0004 | 0.0001 | 0.0003 |
Bud Periphery | 0.0015 | 0.0004 | 0.0006 | 0.0018 | 0.0023 | 0.0004 | 0.0004 | 0.0006 | 0.0008 | 0.0006 | 0.0002 | 0.0001 | 0.0019 | 0.0006 | 0.0003 | 0.0014 | 0.0001 | 0.0002 |
Bud Site | 0.002 | 0.0045 | 0.0045 | 0.0173 | 0.0071 | 0.0007 | 0.002 | 0.0008 | 0.0027 | 0.0007 | 0.0001 | 0.0001 | 0.0038 | 0.0013 | 0.0174 | 0.0003 | 0 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 |
Cytoplasm | 0.0037 | 0.0012 | 0.0012 | 0.0006 | 0.003 | 0.0003 | 0.0034 | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0009 | 0.0094 | 0.0126 | 0.0009 | 0.0001 | 0.0004 |
Cytoplasmic Foci | 0.0061 | 0.003 | 0.0029 | 0.0055 | 0.0046 | 0.0033 | 0.0175 | 0.0036 | 0.0063 | 0.0039 | 0.0032 | 0.0012 | 0.0039 | 0.0222 | 0.0121 | 0.0016 | 0.0056 | 0.0012 |
Eisosomes | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0077 | 0.0022 | 0.0029 | 0.0023 | 0.0031 | 0.001 | 0.0018 | 0.0035 | 0.0016 | 0.0014 | 0.0013 | 0.0008 | 0.0036 | 0.0031 | 0.005 | 0.0028 | 0.0022 | 0.0013 |
Endosome | 0.0235 | 0.0065 | 0.0056 | 0.0077 | 0.0175 | 0.0095 | 0.0281 | 0.0028 | 0.0101 | 0.0031 | 0.0034 | 0.0031 | 0.0206 | 0.0117 | 0.0153 | 0.0044 | 0.0017 | 0.0024 |
Golgi | 0.0108 | 0.0018 | 0.003 | 0.0059 | 0.0141 | 0.0095 | 0.0085 | 0.0016 | 0.0085 | 0.0025 | 0.0042 | 0.0031 | 0.0054 | 0.0045 | 0.0056 | 0.0021 | 0.0038 | 0.0016 |
Lipid Particles | 0.0399 | 0.0189 | 0.0329 | 0.0215 | 0.082 | 0.0261 | 0.0271 | 0.0255 | 0.0435 | 0.0237 | 0.122 | 0.0321 | 0.0243 | 0.0465 | 0.0194 | 0.0245 | 0.1373 | 0.0479 |
Mitochondria | 0.0144 | 0.0019 | 0.0029 | 0.0115 | 0.0179 | 0.0138 | 0.0149 | 0.0022 | 0.0107 | 0.0023 | 0.0032 | 0.0019 | 0.0246 | 0.0066 | 0.0034 | 0.0039 | 0.0011 | 0.0007 |
None | 0.0104 | 0.001 | 0.0042 | 0.0017 | 0.0133 | 0.0008 | 0.009 | 0.0014 | 0.0029 | 0.0029 | 0.0009 | 0.0003 | 0.0037 | 0.0052 | 0.0036 | 0.0131 | 0.0006 | 0.002 |
Nuclear Periphery | 0.6847 | 0.7563 | 0.7239 | 0.7181 | 0.6063 | 0.8159 | 0.7686 | 0.8311 | 0.7852 | 0.7793 | 0.6878 | 0.8974 | 0.8005 | 0.6382 | 0.7329 | 0.7931 | 0.7152 | 0.8644 |
Nucleolus | 0.012 | 0.0326 | 0.0212 | 0.0171 | 0.041 | 0.0175 | 0.0171 | 0.0352 | 0.0176 | 0.0267 | 0.0482 | 0.0057 | 0.0127 | 0.0204 | 0.0114 | 0.0106 | 0.0114 | 0.015 |
Nucleus | 0.0414 | 0.0914 | 0.0953 | 0.0624 | 0.0313 | 0.0248 | 0.0287 | 0.0292 | 0.0287 | 0.0126 | 0.0158 | 0.0172 | 0.0142 | 0.0702 | 0.0675 | 0.024 | 0.004 | 0.0153 |
Peroxisomes | 0.0036 | 0.0016 | 0.0038 | 0.0081 | 0.0032 | 0.004 | 0.0043 | 0.001 | 0.0052 | 0.0031 | 0.0015 | 0.0006 | 0.0042 | 0.0064 | 0.0075 | 0.0018 | 0.0016 | 0.0004 |
Punctate Nuclear | 0.0648 | 0.0547 | 0.0611 | 0.0605 | 0.045 | 0.0503 | 0.0127 | 0.0374 | 0.0496 | 0.1172 | 0.0931 | 0.0176 | 0.0403 | 0.134 | 0.0509 | 0.0769 | 0.0998 | 0.0347 |
Vacuole | 0.004 | 0.0023 | 0.0024 | 0.002 | 0.0074 | 0.0009 | 0.0015 | 0.0009 | 0.0023 | 0.0006 | 0.0007 | 0.0003 | 0.0031 | 0.0015 | 0.0012 | 0.0016 | 0.0003 | 0.0006 |
Vacuole Periphery | 0.0181 | 0.0167 | 0.0189 | 0.0141 | 0.0123 | 0.0098 | 0.013 | 0.019 | 0.0169 | 0.0122 | 0.0126 | 0.0088 | 0.0176 | 0.0098 | 0.0208 | 0.0256 | 0.0145 | 0.0101 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.7408 | 15.0622 | 12.0592 | 16.1833 | 17.8216 | 17.7262 | 16.5319 | 21.572 | 21.1794 | 21.1689 |
Translational Efficiency | 1.6504 | 1.3329 | 1.5486 | 1.3892 | 1.1897 | 1.2914 | 1.165 | 0.9603 | 1.0982 | 1.1064 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
71 | 504 | 841 | 1238 | 1931 | 1972 | 373 | 1491 | 2002 | 2476 | 1214 | 2729 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 905.93 | 794.93 | 1092.43 | 974.53 | 745.40 | 840.59 | 1043.86 | 954.39 | 751.09 | 831.30 | 1077.51 | 963.53 |
Standard Deviation | 1485.58 | 137.99 | 160.31 | 135.18 | 114.72 | 113.86 | 125.01 | 138.26 | 303.06 | 120.58 | 152.01 | 137.24 |
Intensity Change Log 2 | — | -0.188572 | 0.270069 | 0.105307 | — | 0.173387 | 0.485842 | 0.356564 | — | -0.013879 | 0.371479 | 0.224165 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000267 | 0.000501 | 0.000444 | 0.000199 | 0.000225 | 0.000191 | 0.000336 | 0.000169 | 0.000226 | 0.000254 | 0.000411 | 0.000183 |
Bud Neck | 0.001401 | 0.000557 | 0.001875 | 0.001206 | 0.000340 | 0.000456 | 0.001051 | 0.001548 | 0.000378 | 0.000476 | 0.001622 | 0.001393 |
Bud Site | 0.000416 | 0.000336 | 0.000188 | 0.000880 | 0.000198 | 0.000186 | 0.000197 | 0.002286 | 0.000206 | 0.000217 | 0.000191 | 0.001648 |
Cell Periphery | 0.000108 | 0.000072 | 0.000025 | 0.000020 | 0.000053 | 0.000033 | 0.000019 | 0.000021 | 0.000055 | 0.000041 | 0.000023 | 0.000021 |
Cytoplasm | 0.023390 | 0.004022 | 0.000920 | 0.001945 | 0.002728 | 0.001794 | 0.000316 | 0.002130 | 0.003461 | 0.002247 | 0.000734 | 0.002046 |
Cytoplasmic Foci | 0.010264 | 0.004809 | 0.000264 | 0.001309 | 0.003729 | 0.004770 | 0.000121 | 0.000903 | 0.003961 | 0.004778 | 0.000220 | 0.001087 |
Eisosomes | 0.000053 | 0.000025 | 0.000008 | 0.000004 | 0.000016 | 0.000012 | 0.000007 | 0.000005 | 0.000017 | 0.000015 | 0.000008 | 0.000004 |
Endoplasmic Reticulum | 0.006525 | 0.006061 | 0.002930 | 0.002323 | 0.003470 | 0.003291 | 0.003265 | 0.001931 | 0.003578 | 0.003855 | 0.003033 | 0.002109 |
Endosome | 0.008747 | 0.006922 | 0.000561 | 0.002413 | 0.003870 | 0.007883 | 0.000617 | 0.002243 | 0.004043 | 0.007687 | 0.000578 | 0.002320 |
Golgi | 0.001159 | 0.003587 | 0.000220 | 0.001814 | 0.002376 | 0.003455 | 0.000103 | 0.001111 | 0.002333 | 0.003482 | 0.000184 | 0.001430 |
Lipid Particles | 0.157773* | 0.058332 | 0.000449 | 0.001162 | 0.055950 | 0.038844 | 0.001013 | 0.002261 | 0.059561 | 0.042811 | 0.000622 | 0.001762 |
Mitochondria | 0.013950 | 0.008876 | 0.001021 | 0.001726 | 0.006414 | 0.007695 | 0.000695 | 0.001242 | 0.006681 | 0.007935 | 0.000921 | 0.001462 |
Mitotic Spindle | 0.023541 | 0.054226 | 0.173408* | 0.279013* | 0.020686 | 0.053335 | 0.151126* | 0.220963* | 0.020787 | 0.053516 | 0.166562* | 0.247297* |
None | 0.008739 | 0.006520 | 0.000519 | 0.000282 | 0.004829 | 0.001213 | 0.000268 | 0.000536 | 0.004968 | 0.002293 | 0.000442 | 0.000421 |
Nuclear Periphery | 0.051906 | 0.045394 | 0.240826* | 0.174929* | 0.051630 | 0.066128 | 0.184163* | 0.163631* | 0.051640 | 0.061908 | 0.223416* | 0.168756* |
Nuclear Periphery Foci | 0.574580* | 0.744378* | 0.448867* | 0.508196* | 0.795735* | 0.757647* | 0.590275* | 0.570404* | 0.787892* | 0.754946* | 0.492315* | 0.542183* |
Nucleolus | 0.068488 | 0.023425 | 0.055230 | 0.007155 | 0.028419 | 0.025302 | 0.031161 | 0.009297 | 0.029840 | 0.024920 | 0.047835 | 0.008325 |
Nucleus | 0.038806 | 0.027007 | 0.070616 | 0.009872 | 0.017290 | 0.021091 | 0.033786 | 0.012701 | 0.018054 | 0.022296 | 0.059300 | 0.011418 |
Peroxisomes | 0.003881 | 0.002814 | 0.000516 | 0.000161 | 0.001020 | 0.001047 | 0.000270 | 0.000148 | 0.001122 | 0.001406 | 0.000440 | 0.000154 |
Vacuole | 0.002568 | 0.001311 | 0.000636 | 0.003635 | 0.000476 | 0.003678 | 0.001000 | 0.004032 | 0.000550 | 0.003196 | 0.000748 | 0.003852 |
Vacuole Periphery | 0.003436 | 0.000827 | 0.000478 | 0.001757 | 0.000547 | 0.001948 | 0.000210 | 0.002438 | 0.000649 | 0.001720 | 0.000396 | 0.002129 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.45 | -2.37 | 0.60 | 3.69 | 4.42 | 0.76 | -2.57 | 0.57 | -0.25 | 2.98 | -0.75 | -4.69 | 0.39 | 1.49 | 5.94 |
Bud Neck | 1.82 | -1.19 | 0.01 | -4.25 | 2.42 | -1.65 | -4.38 | -6.07 | -5.54 | -0.06 | -1.48 | -9.91 | -7.66 | -7.16 | 1.64 |
Bud Site | 0.75 | 1.12 | -1.22 | -1.72 | -1.92 | 0.03 | -1.73 | -2.45 | -2.46 | -1.91 | -0.18 | -1.57 | -2.93 | -2.93 | -2.59 |
Cell Periphery | 1.32 | 3.31 | 3.52 | 5.38 | 1.67 | 3.87 | 3.25 | 5.98 | 2.79 | 0.74 | 2.62 | 5.57 | 6.97 | 5.70 | 1.63 |
Cytoplasm | 1.78 | 1.95 | 1.87 | 1.11 | -1.36 | 1.18 | 3.65 | -0.30 | -1.21 | -2.88 | 1.66 | 3.13 | 1.07 | -0.65 | -2.56 |
Cytoplasmic Foci | 1.09 | 1.83 | 1.72 | 2.61 | -2.47 | -0.77 | 5.62 | 4.31 | 5.98 | -3.15 | -0.63 | 5.70 | 4.50 | 6.20 | -3.48 |
Eisosomes | 2.22 | 3.72 | 4.09 | 5.93 | 3.87 | 2.60 | 1.64 | 7.73 | 6.04 | 2.11 | 1.35 | 4.79 | 9.49 | 8.93 | 4.08 |
Endoplasmic Reticulum | 0.12 | 1.44 | 1.64 | 4.84 | 1.13 | 0.39 | -0.85 | 4.35 | 2.95 | 3.44 | -0.83 | 0.87 | 4.09 | 4.28 | 3.34 |
Endosome | 0.71 | 3.38 | 2.57 | 3.04 | -5.02 | -3.69 | 5.69 | 2.68 | 5.32 | -3.38 | -3.82 | 7.28 | 3.17 | 6.05 | -6.46 |
Golgi | -2.46 | 4.13 | -0.71 | 1.67 | -2.11 | -1.26 | 5.36 | 1.99 | 2.81 | -1.79 | -1.60 | 5.31 | 1.70 | 3.04 | -2.71 |
Lipid Particles | 3.47 | 5.69 | 5.66 | 9.30 | -3.97 | 4.22 | 17.29 | 16.93 | 13.90 | -1.94 | 4.16 | 18.33 | 17.94 | 16.85 | -4.22 |
Mitochondria | 1.02 | 3.24 | 3.03 | 2.78 | -1.27 | -0.99 | 9.26 | 8.09 | 5.95 | -2.86 | -1.04 | 9.46 | 7.87 | 6.29 | -2.04 |
Mitotic Spindle | -3.25 | -13.59 | -22.13 | -18.73 | -8.09 | -7.87 | -10.38 | -24.48* | -19.54 | -4.31 | -8.63 | -19.22 | -34.92 | -28.11* | -8.39 |
None | 0.33 | 2.44 | 2.55 | 2.91 | 2.22 | 4.65 | 5.90 | 5.38 | 1.91 | -0.65 | 2.91 | 6.01 | 6.09 | 3.76 | 0.59 |
Nuclear Periphery | 0.52 | -15.62 | -11.16 | -21.25 | 7.32 | -5.29 | -14.00 | -23.96 | -20.45 | 1.79 | -4.08 | -28.58 | -30.62 | -28.21 | 8.07 |
Nuclear Periphery Foci | -3.93 | 2.57 | 1.30 | 14.22 | -4.46 | 4.10 | 14.05 | 23.23* | 18.94* | 0.65 | 3.80 | 30.34 | 29.31 | 25.86* | -5.04 |
Nucleolus | 2.28 | 0.67 | 3.29 | 4.69 | 12.21 | 1.25 | -0.58 | 7.93 | 7.09 | 5.19 | 1.76 | -4.87 | 9.16 | 8.84 | 13.08 |
Nucleus | 0.91 | -3.16 | 2.61 | 3.88 | 12.24 | -1.86 | -4.36 | 1.39 | 3.06 | 4.87 | -2.20 | -11.17 | 2.97 | 5.14 | 12.84 |
Peroxisomes | 0.81 | 3.44 | 3.75 | 3.57 | 3.62 | 0.12 | 3.76 | 5.25 | 4.18 | 1.60 | -1.02 | 4.24 | 6.08 | 5.48 | 4.18 |
Vacuole | 0.89 | 1.13 | -1.40 | -3.04 | -3.39 | -4.27 | -2.09 | -4.75 | -1.91 | -0.63 | -4.36 | -2.40 | -6.11 | -2.47 | -3.23 |
Vacuole Periphery | 1.23 | 1.40 | 0.75 | -1.80 | -2.60 | -4.02 | 3.40 | -2.97 | -0.72 | -3.43 | -3.68 | 2.14 | -3.52 | -0.86 | -4.15 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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