Standard name | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | 4 | 0 | 6 | 5 | 5 | 3 | 7 | 7 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 9 | 4 | 7 | 8 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 7 | 4 | 6 | 4 | 5 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 149 | 220 | 233 | 255 | 215 | 273 | 176 | 315 | 222 | 351 | 253 | 225 | 210 | 210 | 247 | 29 | 37 | 31 | 53 | 57 | 32 | 30 | 45 | 32 |
Endoplasmic Reticulum | 2 | 2 | 0 | 1 | 2 | 6 | 9 | 28 | 1 | 8 | 4 | 10 | 2 | 0 | 5 | 9 | 8 | 5 | 4 | 3 | 3 | 4 | 8 | 6 |
Endosome | 27 | 28 | 26 | 2 | 29 | 14 | 1 | 3 | 7 | 2 | 7 | 0 | 5 | 9 | 3 | 0 | 3 | 3 | 14 | 17 | 12 | 13 | 14 | 21 |
Golgi | 2 | 0 | 2 | 0 | 1 | 1 | 0 | 6 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 2 | 1 | 0 | 0 |
Mitochondria | 23 | 29 | 35 | 17 | 26 | 122 | 145 | 138 | 112 | 242 | 133 | 145 | 1 | 1 | 0 | 9 | 9 | 4 | 20 | 22 | 21 | 19 | 23 | 24 |
Nucleus | 37 | 40 | 38 | 8 | 5 | 8 | 6 | 19 | 6 | 3 | 7 | 5 | 40 | 40 | 50 | 17 | 11 | 11 | 31 | 19 | 12 | 9 | 11 | 10 |
Nuclear Periphery | 4 | 4 | 1 | 0 | 4 | 23 | 28 | 43 | 23 | 20 | 10 | 16 | 0 | 0 | 0 | 12 | 13 | 12 | 4 | 3 | 7 | 6 | 8 | 5 |
Nucleolus | 0 | 3 | 1 | 0 | 2 | 4 | 0 | 6 | 0 | 1 | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 5 | 3 | 6 | 9 | 7 |
Vac/Vac Membrane | 300 | 404 | 263 | 134 | 187 | 208 | 126 | 203 | 145 | 187 | 130 | 143 | 154 | 183 | 193 | 115 | 146 | 122 | 375 | 514 | 465 | 369 | 530 | 482 |
Unique Cell Count | 451 | 618 | 494 | 349 | 401 | 521 | 380 | 655 | 414 | 639 | 447 | 444 | 375 | 395 | 445 | 157 | 196 | 160 | 523 | 667 | 574 | 480 | 670 | 613 |
Labelled Cell Count | 544 | 731 | 600 | 422 | 474 | 667 | 500 | 778 | 528 | 831 | 556 | 559 | 415 | 444 | 499 | 194 | 229 | 190 | 523 | 667 | 574 | 480 | 670 | 613 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.2 | 4.4 | 4.1 | 4.9 | 4.8 | 4.8 | 5.7 | 5.3 | 5.0 | 5.4 | 5.5 | 5.6 | 5.6 | 5.9 | 7.0 | 8.2 | 9.4 | 5.9 | 6.1 | 6.3 |
Std Deviation (1e-4) | 0.8 | 1.4 | 1.1 | 0.9 | 1.8 | 2.1 | 2.0 | 2.3 | 2.4 | 2.4 | 2.4 | 2.6 | 0.9 | 0.9 | 1.0 | 1.7 | 2.2 | 2.7 | 1.5 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.14 | 0.11 | 0.11 | 0.37 | 0.25 | 0.17 | 0.28 | 0.31 | 0.34 | 0.34 | 0.42 | 0.65 | 0.88 | 1.09 | 0.42 | 0.46 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7.5 | 1.9 | 1.7 | -0.2 | 0.3 | 1.9 | 2.6 | 2.9 | 1.1 | 2.6 | 1.8 | 2.6 | -6.4 | -6.9 | -6.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.2 | 0 | 0 | -5.1 | -2.9 | -5.3 | -3.1 | -4.9 | -3.1 | -2.3 | -4.1 | -2.9 | -2.2 | -1.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -0.4 | 0 | 0 | 6.6 | 8.1 | 12.0 | 9.1 | 9.9 | -5.0 | -5.1 | -5.7 | -0.6 | -1.2 | -2.1 |
Nucleus | -3.4 | -4.5 | -4.7 | -4.1 | -3.7 | -4.4 | -6.5 | -4.4 | -4.8 | 1.5 | 1.3 | 1.9 | 1.2 | -1.0 | -0.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 5.6 | 5.0 | 3.6 | 2.9 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.3 | -2.0 | -4.3 | -5.9 | -7.6 | -5.5 | -8.2 | -7.5 | -6.5 | -3.6 | -2.0 | -3.0 | 4.4 | 5.1 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0092 | 0.728 | 0.55 | 0.2583 | 1.0228 | 0.4835 | 1.8435 | 2.7527 | 2.8148 | 2.5786 | 2.3718 | 3.1535 | 2.2506 | 3.566 | 2.969 | 2.8609 | 3.7038 | 3.4123 |
Actin | 0.0062 | 0.004 | 0.0002 | 0.0006 | 0.0012 | 0.0001 | 0.0002 | 0.0054 | 0.0001 | 0 | 0.0019 | 0.0003 | 0.0005 | 0.0005 | 0.0027 | 0 | 0.0005 | 0.0015 |
Bud | 0.0023 | 0.0014 | 0.0018 | 0.0164 | 0.0023 | 0.0005 | 0.0005 | 0.001 | 0.0011 | 0.0005 | 0.0025 | 0.001 | 0.0017 | 0.0013 | 0.0031 | 0.0003 | 0.001 | 0.0009 |
Bud Neck | 0.0082 | 0.0026 | 0.0024 | 0.0022 | 0.0012 | 0.003 | 0.0005 | 0.0034 | 0.001 | 0.0012 | 0.0039 | 0.0028 | 0.0057 | 0.0057 | 0.0011 | 0.001 | 0.0005 | 0.0026 |
Bud Periphery | 0.0033 | 0.0018 | 0.0072 | 0.0167 | 0.0043 | 0.0007 | 0.001 | 0.0016 | 0.0013 | 0.0008 | 0.003 | 0.0011 | 0.0042 | 0.0029 | 0.0073 | 0.0006 | 0.0041 | 0.0034 |
Bud Site | 0.008 | 0.0116 | 0.0042 | 0.0119 | 0.0036 | 0.0012 | 0.0007 | 0.0047 | 0.0022 | 0.0011 | 0.003 | 0.0005 | 0.0072 | 0.0098 | 0.0023 | 0.0006 | 0.0006 | 0.0006 |
Cell Periphery | 0.0011 | 0.0011 | 0.0008 | 0.0011 | 0.0009 | 0.0007 | 0.0007 | 0.0011 | 0.001 | 0.0006 | 0.0008 | 0.0009 | 0.0015 | 0.0035 | 0.0014 | 0.0006 | 0.0006 | 0.0015 |
Cytoplasm | 0.1037 | 0.1423 | 0.1148 | 0.1135 | 0.0839 | 0.1478 | 0.1371 | 0.1624 | 0.1158 | 0.1494 | 0.155 | 0.1736 | 0.1806 | 0.2188 | 0.1766 | 0.1228 | 0.1076 | 0.1918 |
Cytoplasmic Foci | 0.0761 | 0.0438 | 0.0545 | 0.0954 | 0.0704 | 0.0565 | 0.0457 | 0.0354 | 0.0466 | 0.0621 | 0.0814 | 0.0535 | 0.0405 | 0.0333 | 0.0459 | 0.0509 | 0.0361 | 0.0521 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0093 | 0.0035 | 0.0039 | 0.0032 | 0.0047 | 0.0042 | 0.0054 | 0.0068 | 0.006 | 0.0022 | 0.0029 | 0.0032 | 0.0745 | 0.0826 | 0.0971 | 0.0038 | 0.1162 | 0.0855 |
Endosome | 0.1572 | 0.0988 | 0.1086 | 0.1021 | 0.2715 | 0.1661 | 0.0976 | 0.0869 | 0.1059 | 0.1371 | 0.132 | 0.1302 | 0.1067 | 0.0661 | 0.112 | 0.178 | 0.2038 | 0.1354 |
Golgi | 0.0051 | 0.0016 | 0.0015 | 0.003 | 0.0031 | 0.0016 | 0.0016 | 0.0015 | 0.0014 | 0.0014 | 0.0064 | 0.0019 | 0.002 | 0.0018 | 0.0024 | 0.0018 | 0.0024 | 0.0027 |
Lipid Particles | 0.0356 | 0.0077 | 0.0113 | 0.0267 | 0.0068 | 0.0111 | 0.0096 | 0.0052 | 0.0082 | 0.0066 | 0.0045 | 0.0087 | 0.0106 | 0.0045 | 0.0137 | 0.0089 | 0.003 | 0.0087 |
Mitochondria | 0.0066 | 0.0045 | 0.0053 | 0.0096 | 0.0037 | 0.0027 | 0.0039 | 0.0036 | 0.0034 | 0.0026 | 0.006 | 0.0039 | 0.0029 | 0.0035 | 0.0043 | 0.003 | 0.0033 | 0.0046 |
None | 0.4345 | 0.4296 | 0.5051 | 0.4087 | 0.2783 | 0.3246 | 0.5364 | 0.4684 | 0.507 | 0.3939 | 0.4499 | 0.3994 | 0.414 | 0.4098 | 0.3617 | 0.3809 | 0.3512 | 0.2888 |
Nuclear Periphery | 0.0101 | 0.005 | 0.0047 | 0.0068 | 0.0155 | 0.0059 | 0.0032 | 0.0033 | 0.0103 | 0.0018 | 0.0017 | 0.0027 | 0.0034 | 0.0039 | 0.0051 | 0.0025 | 0.0034 | 0.0042 |
Nucleolus | 0.0042 | 0.0089 | 0.0052 | 0.0173 | 0.0047 | 0.0061 | 0.0015 | 0.0046 | 0.0028 | 0.0041 | 0.0015 | 0.0033 | 0.0016 | 0.0027 | 0.0017 | 0.0025 | 0.001 | 0.0017 |
Nucleus | 0.0087 | 0.0242 | 0.0117 | 0.0257 | 0.0334 | 0.0167 | 0.0038 | 0.0106 | 0.0083 | 0.0065 | 0.0057 | 0.01 | 0.0051 | 0.0089 | 0.0057 | 0.0067 | 0.0021 | 0.005 |
Peroxisomes | 0.0043 | 0.0013 | 0.002 | 0.0069 | 0.0013 | 0.0012 | 0.0008 | 0.0016 | 0.0076 | 0.0023 | 0.0017 | 0.0011 | 0.0007 | 0.0006 | 0.0012 | 0.0014 | 0.0008 | 0.0008 |
Punctate Nuclear | 0.0059 | 0.0027 | 0.0064 | 0.0055 | 0.0019 | 0.0025 | 0.001 | 0.0011 | 0.0023 | 0.0017 | 0.0009 | 0.0016 | 0.0008 | 0.0009 | 0.0041 | 0.0006 | 0.0006 | 0.0009 |
Vacuole | 0.1003 | 0.191 | 0.1348 | 0.1035 | 0.1857 | 0.2304 | 0.1379 | 0.1816 | 0.1556 | 0.2116 | 0.1275 | 0.1828 | 0.1268 | 0.1307 | 0.1379 | 0.2131 | 0.1442 | 0.1913 |
Vacuole Periphery | 0.0092 | 0.0124 | 0.0137 | 0.0228 | 0.0215 | 0.0164 | 0.0105 | 0.0095 | 0.0117 | 0.0127 | 0.0075 | 0.0176 | 0.0089 | 0.0082 | 0.0126 | 0.0198 | 0.0169 | 0.0159 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.8841 | 31.9026 | 31.4031 | 36.479 | 31.634 | 32.0283 | 35.8773 | 37.544 | 29.4545 | 41.7801 |
Translational Efficiency | 0.7386 | 0.9145 | 0.8845 | 0.6401 | 0.7951 | 0.9789 | 0.677 | 0.6628 | 0.9165 | 0.6954 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
342 | 497 | 294 | 768 | 1839 | 1011 | 349 | 153 | 2181 | 1508 | 643 | 921 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 683.49 | 796.85 | 933.98 | 804.75 | 740.04 | 826.68 | 759.04 | 866.22 | 731.17 | 816.85 | 839.03 | 814.96 |
Standard Deviation | 79.13 | 123.89 | 100.54 | 118.18 | 138.47 | 122.22 | 95.55 | 99.32 | 132.56 | 123.57 | 131.04 | 117.51 |
Intensity Change Log 2 | — | 0.221388 | 0.450471 | 0.235620 | — | 0.159726 | 0.036573 | 0.227130 | — | 0.189661 | 0.250126 | 0.231213 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000046 | 0.000092 | 0.000136 | 0.002788 | 0.000024 | 0.000080 | 0.000452 | 0.000113 | 0.000027 | 0.000084 | 0.000307 | 0.002343 |
Bud Neck | 0.020350 | 0.014549 | 0.005900 | 0.012532 | 0.011651 | 0.011734 | 0.001659 | 0.004344 | 0.013015 | 0.012662 | 0.003598 | 0.011171 |
Bud Site | 0.012775 | 0.013913 | 0.025535 | 0.043118 | 0.013002 | 0.005013 | 0.005617 | 0.018178 | 0.012967 | 0.007946 | 0.014724 | 0.038975 |
Cell Periphery | 0.000141 | 0.000580 | 0.000087 | 0.000177 | 0.001075 | 0.002000 | 0.000116 | 0.000103 | 0.000929 | 0.001532 | 0.000103 | 0.000165 |
Cytoplasm | 0.176208* | 0.044844 | 0.459555* | 0.399201* | 0.036845 | 0.008037 | 0.425312* | 0.315872* | 0.058698 | 0.020168 | 0.440969* | 0.385358* |
Cytoplasmic Foci | 0.037053 | 0.019807 | 0.015103 | 0.022227 | 0.008483 | 0.008336 | 0.014183 | 0.012949 | 0.012963 | 0.012116 | 0.014604 | 0.020686 |
Eisosomes | 0.000062 | 0.000020 | 0.000032 | 0.000042 | 0.000012 | 0.000031 | 0.000058 | 0.000038 | 0.000020 | 0.000028 | 0.000046 | 0.000041 |
Endoplasmic Reticulum | 0.000714 | 0.001923 | 0.002323 | 0.001726 | 0.002340 | 0.004683 | 0.003391 | 0.002062 | 0.002085 | 0.003773 | 0.002903 | 0.001782 |
Endosome | 0.035125 | 0.056055 | 0.005738 | 0.011411 | 0.030730 | 0.034037 | 0.002654 | 0.007676 | 0.031419 | 0.041294 | 0.004065 | 0.010790 |
Golgi | 0.001534 | 0.002352 | 0.000098 | 0.003729 | 0.001228 | 0.003198 | 0.000117 | 0.000350 | 0.001276 | 0.002919 | 0.000109 | 0.003168 |
Lipid Particles | 0.001459 | 0.001009 | 0.001027 | 0.001342 | 0.000601 | 0.001895 | 0.002093 | 0.001001 | 0.000736 | 0.001603 | 0.001605 | 0.001286 |
Mitochondria | 0.000292 | 0.003413 | 0.000127 | 0.004193 | 0.002554 | 0.010779 | 0.000334 | 0.001354 | 0.002199 | 0.008352 | 0.000239 | 0.003722 |
Mitotic Spindle | 0.000290 | 0.003438 | 0.000371 | 0.049187 | 0.000413 | 0.000558 | 0.000455 | 0.003991 | 0.000394 | 0.001507 | 0.000417 | 0.041679 |
None | 0.000623 | 0.000530 | 0.002532 | 0.002302 | 0.000994 | 0.000233 | 0.005677 | 0.007630 | 0.000936 | 0.000331 | 0.004239 | 0.003187 |
Nuclear Periphery | 0.000586 | 0.000392 | 0.001137 | 0.001998 | 0.000140 | 0.000579 | 0.001258 | 0.001012 | 0.000210 | 0.000517 | 0.001203 | 0.001834 |
Nuclear Periphery Foci | 0.000242 | 0.001340 | 0.003502 | 0.001757 | 0.000062 | 0.000169 | 0.001484 | 0.000565 | 0.000090 | 0.000555 | 0.002407 | 0.001559 |
Nucleolus | 0.001443 | 0.002212 | 0.000638 | 0.000666 | 0.000849 | 0.001625 | 0.000659 | 0.000606 | 0.000942 | 0.001818 | 0.000649 | 0.000656 |
Nucleus | 0.126929 | 0.017468 | 0.198983* | 0.166269* | 0.006390 | 0.015482 | 0.431910* | 0.444982* | 0.025292 | 0.016137 | 0.325409* | 0.212570* |
Peroxisomes | 0.000927 | 0.001458 | 0.000339 | 0.002097 | 0.000434 | 0.001739 | 0.000853 | 0.000303 | 0.000511 | 0.001646 | 0.000618 | 0.001799 |
Vacuole | 0.577885* | 0.763591* | 0.276439* | 0.265866* | 0.799511* | 0.845464* | 0.101118 | 0.171353 | 0.764758* | 0.818481* | 0.181280 | 0.250165* |
Vacuole Periphery | 0.005316 | 0.051013 | 0.000396 | 0.007371 | 0.082662 | 0.044329 | 0.000600 | 0.005517 | 0.070533 | 0.046532 | 0.000507 | 0.007063 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.03 | -5.06 | -1.84 | -1.77 | -1.70 | -1.68 | -3.48 | -4.57 | -2.19 | 2.05 | -2.13 | -4.24 | -1.91 | -1.81 | -1.44 |
Bud Neck | 1.28 | 3.95 | 2.72 | 1.84 | -2.62 | -0.05 | 8.40 | 5.76 | 4.73 | -2.69 | 0.19 | 7.77 | 2.82 | 2.43 | -4.20 |
Bud Site | -0.41 | -0.45 | -2.77 | -2.92 | -1.95 | 4.99 | 4.90 | -0.43 | -1.63 | -1.74 | 3.27 | 1.59 | -3.51 | -5.06 | -4.05 |
Cell Periphery | -5.76 | 2.05 | 0.60 | 6.23 | -2.06 | -2.94 | 7.82 | 7.91 | 6.56 | 0.78 | -2.72 | 7.99 | 7.78 | 7.21 | -1.09 |
Cytoplasm | 10.23 | -11.96 | -9.99 | -25.62 | 4.06 | 9.43 | -22.42 | -11.31 | -12.78 | 3.29 | 10.15 | -30.00 | -28.19 | -33.28 | 3.56 |
Cytoplasmic Foci | 3.56 | 5.74 | 5.28 | 1.13 | -0.80 | 0.05 | -2.68 | -1.01 | -0.88 | 0.99 | 0.34 | -0.57 | -1.78 | -1.79 | -1.41 |
Eisosomes | 6.18 | 3.57 | 2.31 | -8.28 | -2.44 | -5.31 | -11.34 | -8.83 | -3.81 | 2.44 | -2.90 | -10.04 | -10.86 | -6.02 | 1.88 |
Endoplasmic Reticulum | -6.39 | -7.15 | -8.22 | -1.01 | 0.99 | -5.11 | -4.68 | -1.29 | 3.95 | 3.58 | -4.93 | -4.61 | -0.77 | 4.93 | 4.59 |
Endosome | -3.76 | 7.23 | 6.73 | 9.47 | -1.42 | -1.11 | 17.89 | 12.22 | 9.92 | -1.48 | -3.68 | 18.68 | 15.28 | 13.76 | -3.05 |
Golgi | -0.70 | 1.07 | -0.21 | 0.71 | -2.47 | -1.93 | 4.64 | 3.90 | 3.01 | -0.77 | -2.19 | 4.16 | -0.27 | 1.70 | -2.49 |
Lipid Particles | 1.75 | 1.81 | 0.38 | -1.57 | -1.64 | -2.81 | -6.87 | -3.72 | 1.45 | 3.54 | -2.78 | -6.23 | -4.57 | 0.87 | 1.76 |
Mitochondria | -1.91 | 4.02 | -2.73 | 0.60 | -2.95 | -3.21 | 2.99 | 2.02 | 3.99 | -1.13 | -3.34 | 3.14 | 0.13 | 3.40 | -2.92 |
Mitotic Spindle | -1.50 | -0.52 | -6.82 | -5.80 | -6.79 | -0.38 | -1.25 | -1.43 | -1.34 | -1.15 | -1.52 | -1.06 | -6.86 | -6.53 | -6.80 |
None | 0.65 | -4.21 | -6.21 | -7.02 | -1.65 | 3.62 | -7.97 | -3.20 | -3.62 | -1.02 | 3.39 | -8.06 | -5.65 | -7.65 | 0.63 |
Nuclear Periphery | 2.35 | -3.16 | -7.28 | -7.92 | -3.46 | -3.23 | -17.05 | -8.65 | -4.38 | 2.18 | -3.16 | -11.64 | -9.71 | -7.78 | -3.07 |
Nuclear Periphery Foci | -1.53 | -8.87 | -3.01 | 0.15 | 5.20 | -5.39 | -9.25 | -6.25 | -5.40 | 4.29 | -1.94 | -12.25 | -3.96 | -1.70 | 4.72 |
Nucleolus | -1.45 | 3.61 | 3.52 | 3.24 | -0.74 | -2.44 | 2.32 | 2.67 | 3.76 | 0.62 | -3.21 | 3.24 | 2.70 | 4.60 | -0.94 |
Nucleus | 9.86 | -4.84 | -4.80 | -21.06 | 1.02 | -3.61 | -30.10 | -15.56 | -15.17 | 0.93 | 3.29 | -28.41 | -23.82 | -25.00 | 8.04 |
Peroxisomes | -1.57 | 2.41 | -0.44 | 0.88 | -2.22 | -1.74 | -0.18 | 2.84 | 2.06 | 1.09 | -2.26 | 0.78 | -1.43 | 1.19 | -1.69 |
Vacuole | -8.79 | 10.52 | 10.77 | 25.11 | -1.46 | -4.66 | 54.83* | 21.93 | 23.32 | -3.60 | -5.98 | 46.60* | 37.08 | 40.53* | -7.18 |
Vacuole Periphery | -9.60 | 4.14 | 1.00 | 9.99 | -3.44 | 6.71 | 19.72 | 17.14 | 9.43 | -1.08 | 5.12 | 19.62 | 18.32 | 13.74 | -3.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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