Standard name | |
---|---|
Human Ortholog | |
Description | Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I. |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 1 | 1 | 3 | 5 |
Bud | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 8 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 3 | 1 | 4 | 5 |
Bud Neck | 1 | 1 | 4 | 3 | 3 | 5 | 3 | 11 | 4 | 14 | 3 | 2 | 5 | 0 | 1 | 0 | 1 | – | 0 | 1 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 1 | 1 | 1 | 0 | 1 | 2 |
Cytoplasm | 191 | 202 | 103 | 115 | 192 | 191 | 250 | 193 | 141 | 190 | 123 | 148 | 211 | 200 | 246 | 297 | 193 | – | 135 | 168 | 67 | 109 | 168 | 203 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 22 | 14 | – | 2 | 0 | 0 | 0 | 0 | 3 |
Endosome | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | – | 2 | 2 | 1 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 24 | 3 | 4 | 4 | 3 | 17 | 47 | 32 | 65 | 65 | 76 | 61 | 1 | 0 | 0 | 7 | 4 | – | 30 | 21 | 17 | 10 | 14 | 12 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 2 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 1 | 6 | 6 | 0 | 4 | 2 | 4 | 1 | 0 | 0 | 1 | 1 | – | 3 | 0 | 6 | 0 | 3 | 3 |
Vac/Vac Membrane | 1 | 4 | 0 | 0 | 1 | 1 | 5 | 6 | 1 | 0 | 0 | 4 | 0 | 2 | 1 | 11 | 9 | – | 3 | 10 | 4 | 1 | 2 | 3 |
Unique Cell Count | 205 | 208 | 109 | 120 | 199 | 199 | 278 | 217 | 172 | 241 | 170 | 188 | 213 | 204 | 247 | 310 | 209 | 196 | 214 | 110 | 131 | 208 | 251 | |
Labelled Cell Count | 219 | 213 | 113 | 126 | 206 | 217 | 323 | 253 | 218 | 284 | 213 | 221 | 218 | 204 | 248 | 343 | 230 | 196 | 214 | 110 | 131 | 208 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | 3.9 | 4.9 | 4.5 | 4.7 | 4.0 | 3.5 | 4.2 | 3.1 | 3.2 | 3.0 | 3.4 | 4.1 | 4.9 | 4.7 | 5.0 | 5.2 | 4.7 | 4.4 | 4.6 | 4.8 |
Std Deviation (1e-4) | 0.7 | 0.9 | 2.2 | 2.4 | 2.2 | 2.1 | 1.8 | 2.3 | 1.8 | 1.3 | 1.4 | 1.3 | 0.8 | 1.8 | 1.5 | 1.0 | 1.3 | 0.9 | 1.6 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.06 | -0.29 | -0.49 | -0.23 | -0.65 | -0.59 | -0.71 | -0.54 | -0.24 | 0.01 | -0.05 | 0.05 | 0.09 | -0.05 | -0.15 | -0.07 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0669 | -0.2448 | -0.0448 | 0.5134 | 0.0834 | 0.0446 | -0.5227 | -0.6373 | -0.5227 | -0.8523 | -0.3491 | -0.6725 | -1.4738 | -1.5833 | -1.6525 | -0.1578 | -1.2101 | -1.7068 |
Actin | 0.0133 | 0.0025 | 0.0022 | 0.0534 | 0.0034 | 0.0004 | 0.0157 | 0.0002 | 0.0214 | 0.0001 | 0.064 | 0.0002 | 0.0107 | 0.0003 | 0.0015 | 0.0087 | 0.0117 | 0.0038 |
Bud | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0014 | 0.0001 | 0.0008 | 0.0023 | 0.0014 | 0 | 0.0003 | 0.002 | 0.002 | 0.001 | 0.0013 | 0.0053 | 0.0035 | 0.0002 |
Bud Neck | 0.0041 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0.0008 | 0.003 | 0.0001 | 0.0027 | 0.0003 | 0.0014 | 0.0005 | 0.0038 | 0.0003 | 0.0001 | 0.0007 | 0.0005 | 0.0019 |
Bud Periphery | 0.0026 | 0.0001 | 0.0003 | 0.0003 | 0.005 | 0.0002 | 0.0021 | 0.006 | 0.0026 | 0 | 0.0005 | 0.0072 | 0.0026 | 0.002 | 0.0034 | 0.0196 | 0.0145 | 0.0003 |
Bud Site | 0.0064 | 0.0005 | 0.0027 | 0.0018 | 0.0022 | 0 | 0.0092 | 0.002 | 0.0037 | 0 | 0.0013 | 0.0002 | 0.0197 | 0.001 | 0.0024 | 0.0034 | 0.0005 | 0.0001 |
Cell Periphery | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0027 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0.0001 |
Cytoplasm | 0.0666 | 0.0714 | 0.0483 | 0.0626 | 0.0162 | 0.07 | 0.03 | 0.0265 | 0.0414 | 0.0294 | 0.0208 | 0.0595 | 0.0371 | 0.0585 | 0.0756 | 0.0564 | 0.1432 | 0.1554 |
Cytoplasmic Foci | 0.0254 | 0.0062 | 0.0182 | 0.0245 | 0.0231 | 0.0022 | 0.016 | 0.0039 | 0.0074 | 0.0008 | 0.0222 | 0.0109 | 0.0365 | 0.0072 | 0.0104 | 0.0283 | 0.0258 | 0.0135 |
Eisosomes | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0008 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0058 | 0.0008 | 0.001 | 0.0009 | 0.0009 | 0.0006 | 0.0039 | 0.0004 | 0.0027 | 0.0003 | 0.0015 | 0.0003 | 0.003 | 0.0009 | 0.0027 | 0.0048 | 0.0026 | 0.0033 |
Endosome | 0.0282 | 0.0009 | 0.018 | 0.0233 | 0.014 | 0.0007 | 0.0105 | 0.0004 | 0.0093 | 0.0004 | 0.0244 | 0.0006 | 0.0183 | 0.0011 | 0.0157 | 0.104 | 0.0146 | 0.0088 |
Golgi | 0.007 | 0.0001 | 0.0083 | 0.0236 | 0.0057 | 0.0001 | 0.0025 | 0.0001 | 0.0031 | 0 | 0.0078 | 0 | 0.0042 | 0.0001 | 0.0046 | 0.0129 | 0.0213 | 0.0023 |
Lipid Particles | 0.0145 | 0.0003 | 0.0057 | 0.0276 | 0.0139 | 0.0002 | 0.0043 | 0.0003 | 0.0028 | 0 | 0.0181 | 0.0001 | 0.0221 | 0.0004 | 0.0055 | 0.0069 | 0.0249 | 0.0042 |
Mitochondria | 0.0088 | 0.0004 | 0.0019 | 0.0054 | 0.0097 | 0.0072 | 0.0051 | 0.0024 | 0.0092 | 0.0004 | 0.0028 | 0.0003 | 0.0055 | 0.0009 | 0.0044 | 0.0899 | 0.0135 | 0.0153 |
None | 0.7418 | 0.8544 | 0.8745 | 0.6897 | 0.7876 | 0.877 | 0.8562 | 0.9348 | 0.8639 | 0.8889 | 0.7535 | 0.8826 | 0.7833 | 0.887 | 0.8425 | 0.4672 | 0.5421 | 0.7435 |
Nuclear Periphery | 0.0095 | 0.0037 | 0.0007 | 0.0015 | 0.0015 | 0.001 | 0.0058 | 0.0008 | 0.0033 | 0.0019 | 0.0136 | 0.0005 | 0.0069 | 0.0006 | 0.0025 | 0.0066 | 0.0133 | 0.0028 |
Nucleolus | 0.0041 | 0.0008 | 0.0002 | 0.0007 | 0.0023 | 0.0013 | 0.0012 | 0.0017 | 0.0008 | 0.0025 | 0.0012 | 0.0007 | 0.0026 | 0.0014 | 0.0005 | 0.0054 | 0.0008 | 0.0003 |
Nucleus | 0.0227 | 0.0403 | 0.0085 | 0.0372 | 0.054 | 0.0321 | 0.0044 | 0.0057 | 0.0091 | 0.0676 | 0.0031 | 0.0309 | 0.0191 | 0.0293 | 0.0131 | 0.1294 | 0.098 | 0.0345 |
Peroxisomes | 0.0221 | 0.0019 | 0.0057 | 0.0397 | 0.0529 | 0.0004 | 0.0046 | 0.0027 | 0.0079 | 0 | 0.0469 | 0.0001 | 0.0049 | 0.0006 | 0.0077 | 0.0014 | 0.0619 | 0.0028 |
Punctate Nuclear | 0.0078 | 0.0144 | 0.0018 | 0.0033 | 0.0023 | 0.005 | 0.0173 | 0.0087 | 0.0039 | 0.007 | 0.0141 | 0.0027 | 0.0104 | 0.0062 | 0.0016 | 0.0124 | 0.0018 | 0.0044 |
Vacuole | 0.005 | 0.0004 | 0.0009 | 0.0025 | 0.0027 | 0.0004 | 0.0032 | 0.0006 | 0.0015 | 0.0002 | 0.0009 | 0.0005 | 0.0044 | 0.0008 | 0.0031 | 0.0274 | 0.0023 | 0.002 |
Vacuole Periphery | 0.0022 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0001 | 0.001 | 0.0002 | 0.0005 | 0 | 0.0011 | 0.0001 | 0.0017 | 0.0001 | 0.0008 | 0.0082 | 0.003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.0118 | 13.1894 | 16.7778 | 12.5926 | 8.8815 | 9.4796 | 10.7162 | 13.1796 | 10.4034 | 11.9342 |
Translational Efficiency | 1.1916 | 0.8155 | 0.5503 | 0.7168 | 0.9694 | 1.4927 | 0.8963 | 0.6247 | 0.8214 | 0.948 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|