Standard name | |
---|---|
Human Ortholog | |
Description | Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 73 | 73 | 156 | 79 | 92 | 39 | 90 | 70 | 56 | 60 | 76 | 117 | 19 | 11 | 13 | – | – | – | 31 | 26 | 23 | 22 | 26 | 34 |
Bud | 11 | 7 | 16 | 11 | 22 | 34 | 42 | 24 | 34 | 39 | 42 | 49 | 14 | 9 | 6 | – | – | – | 0 | 2 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 5 | 7 | 5 | 2 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 1 | 5 | 2 | 5 | 2 | 2 | 5 | 1 | 4 | 8 | 2 | 3 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 23 | 20 | 22 | 19 | 13 | 10 | 14 | 15 | 22 | 29 | 17 | 14 | 16 | 18 | – | – | – | 0 | 5 | 2 | 2 | 0 | 1 |
Endoplasmic Reticulum | 14 | 52 | 29 | 13 | 14 | 14 | 21 | 7 | 17 | 11 | 36 | 40 | 21 | 12 | 11 | – | – | – | 2 | 5 | 2 | 1 | 3 | 1 |
Endosome | 40 | 30 | 56 | 44 | 58 | 121 | 97 | 90 | 59 | 77 | 66 | 145 | 15 | 49 | 43 | – | – | – | 77 | 81 | 159 | 33 | 29 | 26 |
Golgi | 200 | 164 | 293 | 238 | 163 | 136 | 138 | 138 | 73 | 68 | 65 | 127 | 161 | 228 | 207 | – | – | – | 244 | 212 | 345 | 141 | 197 | 200 |
Mitochondria | 177 | 127 | 74 | 106 | 132 | 300 | 358 | 174 | 403 | 428 | 366 | 267 | 111 | 63 | 91 | – | – | – | 11 | 22 | 29 | 9 | 12 | 6 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 1 | 0 | 2 | 6 | 3 | 8 | 4 | 5 | 6 | 1 | 1 | 1 | – | – | – | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 3 | 2 | 4 | 1 | 8 | 14 | 34 | 50 | 31 | 32 | 40 | 51 | 0 | 11 | 9 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 2 | 1 | 5 | 29 | 13 | 7 | 10 | 9 | 6 | 17 | 3 | 4 | 5 | – | – | – | 15 | 8 | 23 | 9 | 21 | 24 |
SpindlePole | 4 | 2 | 29 | 19 | 40 | 34 | 43 | 43 | 18 | 34 | 28 | 64 | 1 | 9 | 3 | – | – | – | 3 | 4 | 5 | 3 | 1 | 0 |
Vac/Vac Membrane | 13 | 23 | 36 | 14 | 14 | 38 | 21 | 42 | 18 | 27 | 38 | 52 | 12 | 21 | 14 | – | – | – | 6 | 14 | 7 | 2 | 0 | 0 |
Unique Cell Count | 333 | 329 | 480 | 358 | 382 | 544 | 581 | 488 | 539 | 612 | 594 | 664 | 273 | 348 | 312 | 401 | 387 | 605 | 230 | 299 | 297 | |||
Labelled Cell Count | 547 | 509 | 717 | 555 | 571 | 782 | 879 | 668 | 754 | 821 | 809 | 962 | 374 | 438 | 422 | 401 | 387 | 605 | 230 | 299 | 297 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 10.2 | 10.1 | 9.6 | 9.3 | 8.8 | 8.5 | 8.9 | 8.0 | 7.9 | 7.7 | 8.5 | 11.6 | 12.0 | 12.1 | – | – | – | 9.7 | 10.2 | 10.5 |
Std Deviation (1e-4) | 1.7 | 1.7 | 1.2 | 1.2 | 1.5 | 1.3 | 1.2 | 1.4 | 1.4 | 1.5 | 1.4 | 1.5 | 1.7 | 1.7 | 1.9 | – | – | – | 1.6 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.12 | -0.2 | -0.24 | -0.18 | -0.33 | -0.36 | -0.38 | -0.24 | 0.21 | 0.26 | 0.26 | – | – | – | -0.05 | 0.02 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.3 | -2.7 | -10.3 | -6.5 | -6.7 | -8.7 | -9.3 | -7.8 | -5.8 | -8.0 | -10.4 | -9.5 | – | – | – |
Bud | -0.2 | 1.7 | 2.2 | 2.8 | 1.2 | 2.2 | 2.3 | 2.7 | 2.9 | 1.2 | -0.6 | -1.2 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 1.3 | 0.6 | -1.6 | -2.4 | -1.1 | -1.2 | -0.5 | 0.6 | -1.5 | 0.6 | 0.3 | 1.0 | – | – | – |
Endoplasmic Reticulum | -1.6 | -1.6 | -2.8 | -1.9 | -3.8 | -2.2 | -3.7 | 0 | 0 | 0.9 | -1.7 | -1.6 | – | – | – |
Endosome | 0.3 | 1.5 | 4.5 | 2.3 | 2.9 | -0.4 | 0.5 | -0.3 | 4.5 | -2.8 | 1.0 | 0.9 | – | – | – |
Golgi | 1.6 | -5.4 | -11.7 | -12.3 | -10.3 | -15.8 | -17.4 | -17.3 | -14.5 | -0.6 | 1.3 | 1.5 | – | – | – |
Mitochondria | 4.9 | 6.5 | 13.2 | 15.2 | 7.2 | 19.0 | 17.9 | 15.3 | 9.0 | 7.7 | 1.0 | 4.7 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 1.6 | 2.1 | 4.4 | 6.4 | 4.3 | 4.0 | 4.9 | 5.3 | 0 | 2.5 | 2.2 | – | – | – |
Peroxisomes | 0 | 0 | 4.6 | 2.5 | 0 | 2.1 | 0 | 0 | 2.8 | 0 | 0 | 0 | – | – | – |
SpindlePole | -0.5 | 2.4 | 0.1 | 0.9 | 1.6 | -2.1 | -0.3 | -1.0 | 2.2 | -3.8 | -2.3 | -3.5 | – | – | – |
Vacuole | -2.2 | -2.4 | -0.3 | -2.8 | 0.6 | -3.0 | -2.2 | -0.7 | 0.2 | -1.7 | -0.8 | -1.7 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7304 | 7.7774 | 7.0218 | 6.6468 | 5.7725 | 6.4576 | 8.3015 | 9.9889 | 9.0251 | 9.0616 | 8.5232 | 9.4413 | 7.2186 | 9.2846 | 8.6351 | 7.7728 | 8.2601 | 8.5175 |
Actin | 0.047 | 0.0115 | 0.009 | 0.0026 | 0.0301 | 0.0202 | 0.0426 | 0.0105 | 0.0137 | 0.0015 | 0.0015 | 0.0071 | 0.0488 | 0.0321 | 0.0286 | 0.0047 | 0.0145 | 0.0156 |
Bud | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0005 | 0.0018 | 0.0001 | 0.0017 | 0.0001 |
Bud Neck | 0.0013 | 0.0005 | 0.0005 | 0.0003 | 0.0014 | 0.0009 | 0.0008 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.002 | 0.0006 | 0.0006 | 0.0003 | 0.0012 | 0.0004 |
Bud Periphery | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0.0002 | 0.0019 | 0.0001 | 0.0038 | 0.0001 |
Bud Site | 0.0019 | 0.0071 | 0.0014 | 0.0003 | 0.0018 | 0.0005 | 0.0021 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0047 | 0.0027 | 0.0017 | 0.0002 | 0.0027 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 |
Cytoplasm | 0.0137 | 0.012 | 0.0045 | 0.0025 | 0.0047 | 0.0044 | 0.0037 | 0.0093 | 0.0041 | 0.0024 | 0.0092 | 0.002 | 0.0068 | 0.0217 | 0.0243 | 0.0029 | 0.0036 | 0.0021 |
Cytoplasmic Foci | 0.1207 | 0.1963 | 0.1583 | 0.1303 | 0.1214 | 0.0973 | 0.1299 | 0.1901 | 0.1455 | 0.0852 | 0.12 | 0.0994 | 0.1362 | 0.1969 | 0.1442 | 0.1006 | 0.1086 | 0.0864 |
Eisosomes | 0.0007 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0003 | 0.0009 | 0.0004 | 0.0012 | 0.0023 | 0.0016 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0051 | 0.0012 | 0.0027 | 0.0004 | 0.0017 | 0.0009 |
Endosome | 0.2349 | 0.2323 | 0.2556 | 0.3432 | 0.2584 | 0.1878 | 0.2211 | 0.2476 | 0.1977 | 0.2554 | 0.2395 | 0.1382 | 0.2372 | 0.2694 | 0.2557 | 0.3243 | 0.327 | 0.1762 |
Golgi | 0.4794 | 0.5024 | 0.5092 | 0.494 | 0.5115 | 0.6201 | 0.5435 | 0.5215 | 0.5975 | 0.6463 | 0.6196 | 0.7401 | 0.4224 | 0.4262 | 0.5125 | 0.553 | 0.4463 | 0.6884 |
Lipid Particles | 0.0258 | 0.0056 | 0.0154 | 0.0069 | 0.004 | 0.0248 | 0.0146 | 0.0021 | 0.0067 | 0.0018 | 0.0018 | 0.0016 | 0.0154 | 0.007 | 0.0025 | 0.0026 | 0.0169 | 0.0047 |
Mitochondria | 0.0188 | 0.0056 | 0.0132 | 0.0023 | 0.0515 | 0.0223 | 0.0107 | 0.0027 | 0.0047 | 0.0028 | 0.0008 | 0.0037 | 0.0271 | 0.0021 | 0.0043 | 0.0018 | 0.025 | 0.0041 |
None | 0.0087 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0002 |
Nuclear Periphery | 0.0078 | 0.0001 | 0.0031 | 0.0002 | 0.0003 | 0.0018 | 0.0035 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0087 | 0.0004 | 0.0011 | 0.0001 | 0.0019 | 0.0092 |
Nucleolus | 0.0036 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0.0003 | 0 | 0 | 0.0004 | 0.0001 |
Nucleus | 0.0026 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0009 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0031 | 0.0002 | 0.0024 | 0 | 0.0015 | 0.0007 |
Peroxisomes | 0.0144 | 0.0224 | 0.0209 | 0.0041 | 0.0065 | 0.0116 | 0.0135 | 0.0122 | 0.0259 | 0.001 | 0.0049 | 0.0046 | 0.0592 | 0.0275 | 0.0086 | 0.0045 | 0.0059 | 0.0053 |
Punctate Nuclear | 0.0097 | 0.0002 | 0.0025 | 0.0001 | 0.0003 | 0.0008 | 0.0057 | 0.0002 | 0.001 | 0.0001 | 0.0001 | 0.0007 | 0.0059 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0032 |
Vacuole | 0.0017 | 0.0012 | 0.002 | 0.0099 | 0.0026 | 0.0016 | 0.0013 | 0.0009 | 0.0006 | 0.0012 | 0.0008 | 0.0004 | 0.0087 | 0.0085 | 0.004 | 0.0019 | 0.0256 | 0.0008 |
Vacuole Periphery | 0.0022 | 0.0009 | 0.002 | 0.0023 | 0.0023 | 0.0017 | 0.0014 | 0.0009 | 0.0008 | 0.0014 | 0.0009 | 0.0006 | 0.0041 | 0.0019 | 0.0022 | 0.0023 | 0.0102 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 63.1604 | 33.5595 | 48.4591 | 65.2215 | 51.6336 | 46.1399 | 53.076 | 57.0997 | 56.9456 | 73.9282 |
Translational Efficiency | 0.8483 | 1.1547 | 0.8867 | 0.7943 | 0.8763 | 1.1585 | 0.9174 | 0.7271 | 0.8934 | 0.735 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1596 | 391 | 197 | 1001 | 1800 | 1424 | 1153 | 18 | 3396 | 1815 | 1350 | 1019 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 858.76 | 1224.29 | 1822.25 | 1333.47 | 936.04 | 1108.67 | 1077.97 | 1419.46 | 899.72 | 1133.58 | 1186.58 | 1334.99 |
Standard Deviation | 107.70 | 200.04 | 224.22 | 205.50 | 190.07 | 162.24 | 189.08 | 177.01 | 161.52 | 177.57 | 326.97 | 205.34 |
Intensity Change Log 2 | — | 0.511618 | 1.085394 | 0.634858 | — | 0.244188 | 0.203675 | 0.600700 | — | 0.378338 | 0.692339 | 0.617145 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007682 | 0.006481 | 0.022180 | 0.031139 | 0.005210 | 0.004232 | 0.001887 | 0.000629 | 0.006372 | 0.004717 | 0.004848 | 0.030600 |
Bud Neck | 0.000509 | 0.000455 | 0.006643 | 0.001884 | 0.001437 | 0.000348 | 0.006693 | 0.000999 | 0.001001 | 0.000371 | 0.006686 | 0.001868 |
Bud Site | 0.004535 | 0.005547 | 0.006252 | 0.057166 | 0.004411 | 0.005014 | 0.008966 | 0.009151 | 0.004469 | 0.005129 | 0.008570 | 0.056318 |
Cell Periphery | 0.000133 | 0.000064 | 0.000204 | 0.000174 | 0.000161 | 0.000058 | 0.000186 | 0.000027 | 0.000148 | 0.000059 | 0.000189 | 0.000172 |
Cytoplasm | 0.002818 | 0.001768 | 0.000856 | 0.059144 | 0.002179 | 0.001931 | 0.019754 | 0.011107 | 0.002480 | 0.001896 | 0.016996 | 0.058295 |
Cytoplasmic Foci | 0.247314* | 0.276348* | 0.178844 | 0.266922* | 0.219148* | 0.283911* | 0.336216* | 0.359685* | 0.232385* | 0.282281* | 0.313251* | 0.268560* |
Eisosomes | 0.000082 | 0.000040 | 0.000089 | 0.000053 | 0.000074 | 0.000037 | 0.000076 | 0.000008 | 0.000078 | 0.000038 | 0.000078 | 0.000052 |
Endoplasmic Reticulum | 0.000535 | 0.000344 | 0.001898 | 0.000349 | 0.000491 | 0.000340 | 0.000603 | 0.000078 | 0.000512 | 0.000341 | 0.000792 | 0.000344 |
Endosome | 0.167201 | 0.161356 | 0.175556 | 0.145535 | 0.187632 | 0.148819 | 0.275150* | 0.248233* | 0.178030 | 0.151520 | 0.260617* | 0.147349 |
Golgi | 0.444541* | 0.511577* | 0.429270* | 0.304173* | 0.506446* | 0.509192* | 0.104239 | 0.141309* | 0.477353* | 0.509706* | 0.151670* | 0.301296* |
Lipid Particles | 0.014750 | 0.003451 | 0.052675 | 0.005719 | 0.014274 | 0.006913 | 0.059637 | 0.008746 | 0.014498 | 0.006167 | 0.058621 | 0.005772 |
Mitochondria | 0.063745 | 0.005952 | 0.001210 | 0.002950 | 0.030923 | 0.011000 | 0.005462 | 0.000056 | 0.046348 | 0.009912 | 0.004842 | 0.002899 |
Mitotic Spindle | 0.004821 | 0.008786 | 0.037948 | 0.055363 | 0.002891 | 0.007620 | 0.090502 | 0.179777* | 0.003798 | 0.007871 | 0.082833 | 0.057561 |
None | 0.000201 | 0.000015 | 0.000039 | 0.000031 | 0.000512 | 0.000275 | 0.003793 | 0.000010 | 0.000366 | 0.000219 | 0.003245 | 0.000031 |
Nuclear Periphery | 0.000064 | 0.000026 | 0.002573 | 0.000077 | 0.000056 | 0.000059 | 0.000504 | 0.000115 | 0.000060 | 0.000052 | 0.000806 | 0.000078 |
Nuclear Periphery Foci | 0.001210 | 0.000158 | 0.014810 | 0.000800 | 0.003575 | 0.000290 | 0.011335 | 0.002193 | 0.002464 | 0.000262 | 0.011842 | 0.000825 |
Nucleolus | 0.000173 | 0.000021 | 0.000037 | 0.000032 | 0.001178 | 0.000115 | 0.003879 | 0.000024 | 0.000706 | 0.000095 | 0.003318 | 0.000032 |
Nucleus | 0.000139 | 0.000019 | 0.000060 | 0.000095 | 0.000628 | 0.000978 | 0.005853 | 0.000022 | 0.000398 | 0.000771 | 0.005008 | 0.000093 |
Peroxisomes | 0.032626 | 0.006954 | 0.005495 | 0.025717 | 0.011148 | 0.007156 | 0.038619 | 0.009840 | 0.021242 | 0.007112 | 0.033786 | 0.025437 |
Vacuole | 0.003246 | 0.006260 | 0.054602 | 0.036800 | 0.004014 | 0.006189 | 0.023781 | 0.026018 | 0.003653 | 0.006204 | 0.028278 | 0.036609 |
Vacuole Periphery | 0.003674 | 0.004379 | 0.008757 | 0.005877 | 0.003610 | 0.005523 | 0.002863 | 0.001973 | 0.003640 | 0.005276 | 0.003723 | 0.005808 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.74 | -2.52 | -6.48 | -6.76 | -1.31 | 0.95 | 3.69 | 5.21 | 4.22 | 1.72 | 1.85 | 1.35 | -7.03 | -7.53 | -7.33 |
Bud Neck | 0.30 | -6.52 | -9.03 | -7.44 | 4.75 | 3.68 | -6.28 | 1.16 | -2.60 | 6.94 | 3.76 | -8.12 | -4.86 | -10.15 | 6.53 |
Bud Site | -0.79 | -1.64 | -15.94 | -15.07 | -15.30 | -0.47 | -4.20 | -1.83 | -1.54 | 0.03 | -0.68 | -4.72 | -16.08 | -15.57 | -14.58 |
Cell Periphery | 3.94 | -3.37 | -2.82 | -7.53 | 1.27 | 2.25 | -0.43 | 2.95 | 3.69 | 3.03 | 3.48 | -0.87 | -1.10 | -10.20 | 0.34 |
Cytoplasm | 1.34 | 2.86 | -13.97 | -14.32 | -14.58 | 0.43 | -7.19 | -2.14 | -2.21 | 2.09 | 1.23 | -6.95 | -14.17 | -14.34 | -9.35 |
Cytoplasmic Foci | -2.94 | 6.27 | -2.39 | 1.17 | -7.37 | -10.74 | -15.01 | -3.10 | -1.67 | -0.57 | -9.93 | -11.82 | -5.34 | 2.39 | 5.53 |
Eisosomes | 8.03 | -0.41 | 6.04 | -2.43 | 2.26 | 3.03 | -0.30 | 5.42 | 7.03 | 10.17 | 5.77 | -0.21 | 3.69 | -3.20 | 4.30 |
Endoplasmic Reticulum | 1.56 | -4.43 | 1.69 | -0.11 | 5.34 | 1.92 | -1.72 | 6.26 | 4.30 | 9.87 | 2.35 | -3.54 | 2.56 | -0.09 | 7.02 |
Endosome | 0.68 | -0.83 | 4.58 | 2.24 | 3.31 | 7.61 | -10.31 | -1.44 | -2.38 | 0.50 | 6.42 | -11.58 | 7.02 | 1.51 | 15.21 |
Golgi | -5.12 | 0.82 | 15.10 | 15.19 | 6.44 | -0.37 | 59.92* | 7.81 | 7.86 | -0.81 | -4.86 | 48.86* | 20.35 | 22.18 | -13.89 |
Lipid Particles | 5.88 | -5.31 | 4.48 | -2.78 | 6.62 | 3.70 | -10.74 | 1.18 | -0.42 | 8.65 | 5.49 | -12.00 | 5.93 | -0.36 | 14.65 |
Mitochondria | 13.81 | 16.35 | 15.68 | 1.66 | -2.07 | 7.23 | 9.97 | 12.79 | 8.25 | 6.13 | 14.69 | 17.77 | 18.67 | 5.41 | 1.91 |
Mitotic Spindle | -1.40 | -4.05 | -11.55 | -9.25 | -2.29 | -3.02 | -14.60 | -2.60 | -2.53 | -1.29 | -3.03 | -14.98 | -12.01 | -10.85 | 3.10 |
None | 1.87 | 1.63 | 1.68 | -4.09 | 0.86 | 0.75 | -3.12 | 2.68 | 1.13 | 3.55 | 0.65 | -3.21 | 3.03 | 1.01 | 3.53 |
Nuclear Periphery | 1.63 | -5.14 | -0.88 | -6.57 | 5.10 | -0.15 | -4.82 | -1.92 | -1.80 | 4.03 | 0.48 | -6.88 | -1.74 | -2.56 | 6.66 |
Nuclear Periphery Foci | 1.92 | -3.15 | 0.54 | -5.89 | 3.24 | 3.60 | -5.12 | 0.91 | -1.54 | 5.37 | 4.01 | -6.68 | 2.76 | -5.10 | 8.00 |
Nucleolus | 1.62 | 1.47 | 1.50 | -0.91 | 0.21 | 2.82 | -3.79 | 3.10 | 1.59 | 5.01 | 2.96 | -4.21 | 3.32 | 1.35 | 5.00 |
Nucleus | 2.63 | 1.81 | -0.21 | -4.66 | -3.44 | -0.54 | -4.40 | 2.60 | 1.56 | 5.00 | -0.76 | -4.59 | 1.96 | 1.30 | 4.87 |
Peroxisomes | 9.10 | 9.26 | 2.35 | -7.47 | -7.67 | 2.94 | -11.21 | 0.42 | -0.82 | 7.72 | 9.54 | -5.70 | -1.66 | -8.66 | 3.53 |
Vacuole | -2.49 | -8.15 | -14.70 | -12.40 | 2.02 | -2.89 | -10.26 | -3.37 | -3.02 | -0.18 | -4.05 | -12.94 | -14.73 | -13.42 | -3.67 |
Vacuole Periphery | -0.62 | -3.94 | -2.80 | -1.11 | 2.22 | -2.33 | 2.12 | 2.68 | 3.78 | 1.37 | -2.37 | -0.05 | -3.13 | -0.44 | -3.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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