Standard name
Human Ortholog
Description Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0.12 0.15 0.08 0.15 0.12 0.13 – 0.13 0.13 0.15 0 0 0 0.12 0.1 0.06 0 0.06 0 0 0 0
Bud 0.06 0.05 0 0 0 0.05 0.06 0.06 – 0.07 0.08 0.08 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.17 0.09 0.17 0.17 0.12 0.14 0.11 0.11 – 0.11 0.1 0.15 0.22 0.22 0.26 0.31 0.22 0.12 0 0 0 0.06 0.13 0.14
Cytoplasm 0.13 0.34 0.11 0.12 0.17 0.19 0.31 0.43 – 0.49 0.55 0.59 0.16 0.16 0.18 0.44 0.54 0.65 0 0.07 0 0 0 0
Endoplasmic Reticulum 0.67 0.61 0.81 0.82 0.78 0.73 0.59 0.4 – 0.35 0.22 0.26 0.72 0.72 0.74 0 0 0.12 0.82 0.76 0.9 0.82 0.75 0.74
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0.19 0.08 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 17 43 37 25 62 51 53 – 46 38 49 11 13 9 2 6 1 2 15 12 10 17 21
Bud 5 13 5 6 5 21 24 27 – 25 23 25 2 2 2 0 4 1 1 2 1 1 3 7
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 7 3 4 7 11
Bud Site 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 15 22 60 41 37 58 47 46 – 39 31 48 65 65 81 5 13 2 2 6 8 21 58 91
Cytoplasm 12 81 37 28 52 77 129 179 – 178 164 189 48 48 55 7 32 11 4 17 3 13 8 13
Endoplasmic Reticulum 60 146 286 200 243 297 246 167 – 127 65 84 214 214 231 0 0 2 96 200 330 303 341 478
Endosome 0 0 1 0 0 1 4 9 – 4 7 2 0 0 0 0 0 0 0 1 1 1 0 0
Golgi 2 0 2 0 6 2 3 0 – 1 1 2 0 0 0 0 1 0 0 2 0 0 0 1
Mitochondria 0 2 1 3 2 2 0 1 – 5 2 1 0 0 0 1 2 1 1 1 2 3 4 4
Nucleus 0 0 0 0 0 0 0 0 – 0 0 2 2 2 2 1 2 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 3 1 2 – 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 1 1 4 – 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 – 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 3 2 0 4 12 4 14 14 – 15 16 10 1 1 6 3 5 1 2 4 0 8 7 14
Unique Cell Count 89 239 351 243 313 405 420 418 364 296 320 298 296 311 16 59 17 118 262 368 372 453 650
Labelled Cell Count 99 283 435 320 384 528 521 502 445 350 415 343 345 386 20 66 19 118 262 368 372 453 650


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.7 13.9 14.5 15.8 20.6 20.0 19.5 17.9 – 16.2 15.0 15.5 13.9 13.9 14.5 16.3 19.3 22.5 15.6 18.1 20.1
Std Deviation (1e-4) 2.9 2.9 2.8 3.0 4.1 4.1 3.5 3.5 – 3.6 3.0 2.9 2.0 2.0 2.1 4.7 7.0 8.3 2.6 3.5 4.2
Intensity Change (Log2) – – – 0.13 0.51 0.47 0.43 0.31 – 0.16 0.05 0.1 -0.06 -0.06 0.0 0.18 0.42 0.63 0.11 0.32 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -1.8 1.2 0 0.2 – 0.2 0.2 1.2 -3.9 -3.5 -4.5 0 -0.5 0
Bud 0 0 2.8 3.1 3.5 – 3.6 4.0 4.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.9 -1.0 -2.4 -2.4 – -2.5 -2.4 -0.7 1.5 1.6 2.8 0 0.9 0
Cytoplasm 0.4 2.3 3.2 6.8 9.9 – 11.2 12.3 13.3 2.1 2.1 2.7 0 8.3 0
Endoplasmic Reticulum 0.3 -1.2 -2.7 -6.8 -11.7 – -12.6 -15.1 -14.4 -2.9 -2.8 -2.2 -7.7 -12.6 -6.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 0 3.5 3.5 – 3.8 4.4 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.7536 13.2825 12.2676 13.8709 11.4248 12.8665 13.7343 16.3627 15.9572 16.4712 13.0755 14.855 15.3551 17.096 16.3344 16.2254 15.0805 15.6563
Actin 0.0484 0.0016 0.0431 0.0044 0.0458 0.0161 0.017 0.0001 0.0123 0.0005 0.0386 0.0008 0.0235 0.0001 0.0051 0.0012 0.0128 0.0005
Bud 0.0003 0.0026 0.0005 0.0003 0.0006 0.0002 0.0005 0 0.0001 0 0.0005 0 0.0001 0 0.0001 0 0.0002 0
Bud Neck 0.0013 0.0007 0.002 0.0005 0.0016 0.0017 0.0019 0.0001 0.0011 0.0002 0.0006 0.0005 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002
Bud Periphery 0.0005 0.0017 0.0016 0.0017 0.0018 0.0006 0.0009 0.0005 0.0004 0.0001 0.0006 0.0002 0.0002 0.0001 0.0004 0.0001 0.0003 0
Bud Site 0.0017 0.0091 0.0035 0.0002 0.0014 0.0005 0.0076 0.0002 0.0008 0 0.0028 0.0001 0.0002 0.0001 0.0004 0 0.0023 0
Cell Periphery 0.0042 0.014 0.0118 0.0219 0.0142 0.0024 0.0044 0.0082 0.0102 0.0041 0.0008 0.0017 0.003 0.0145 0.0044 0.0204 0.002 0.001
Cytoplasm 0.064 0.0921 0.0884 0.0239 0.0353 0.0382 0.0068 0.0226 0.0157 0.0046 0.0094 0.0083 0.0127 0.0211 0.006 0.0083 0.0055 0.0088
Cytoplasmic Foci 0.0112 0.0013 0.0042 0.0005 0.0068 0.0026 0.0166 0.0003 0.0052 0.0006 0.033 0.0018 0.0047 0.0003 0.0014 0.0005 0.0116 0.0006
Eisosomes 0.0004 0.0001 0.0016 0.0001 0.0006 0.0001 0.0003 0 0.0001 0 0.0002 0.0001 0.0003 0 0.0002 0.0001 0.0003 0
Endoplasmic Reticulum 0.7534 0.833 0.7976 0.8767 0.7526 0.8779 0.8479 0.9553 0.9187 0.9754 0.7493 0.9536 0.913 0.9435 0.94 0.9562 0.8916 0.9726
Endosome 0.025 0.001 0.0044 0.0023 0.0262 0.0158 0.0229 0.0006 0.0068 0.005 0.0595 0.0053 0.0036 0.0002 0.0037 0.0013 0.0254 0.0043
Golgi 0.0094 0.0006 0.0036 0.0015 0.0158 0.0053 0.0122 0.0001 0.0084 0.0012 0.0191 0.0026 0.0054 0.0002 0.0025 0.0015 0.0145 0.0004
Lipid Particles 0.0225 0.0134 0.0105 0.0333 0.0243 0.0127 0.0203 0.0049 0.0064 0.0014 0.0263 0.0029 0.0131 0.0135 0.0032 0.0052 0.0066 0.0024
Mitochondria 0.0071 0.0001 0.0041 0.0002 0.0036 0.0029 0.008 0 0.0062 0.0001 0.0099 0.0135 0.0023 0 0.0241 0 0.006 0.0001
None 0.0034 0.0003 0.0027 0.0001 0.0066 0.0005 0.0013 0.0003 0.0001 0.0007 0.0017 0.0003 0.0006 0 0.0002 0.0001 0.0003 0.0002
Nuclear Periphery 0.0202 0.0132 0.0058 0.0051 0.0235 0.0108 0.008 0.0024 0.0028 0.0041 0.0062 0.0018 0.0048 0.0022 0.0036 0.0019 0.0025 0.0065
Nucleolus 0.0004 0.0002 0.0002 0 0.0014 0.0003 0.0006 0 0 0 0.0002 0.0001 0 0 0 0 0 0
Nucleus 0.0034 0.0089 0.0025 0.0005 0.0045 0.002 0.001 0.0002 0.0001 0.0001 0.0008 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0004
Peroxisomes 0.0127 0 0.006 0 0.0017 0.0029 0.0113 0 0.001 0 0.0311 0.0005 0.0103 0 0.0003 0 0.0154 0
Punctate Nuclear 0.0047 0.0003 0.0022 0.0001 0.0222 0.0015 0.0013 0.0001 0.0001 0.0001 0.0039 0.0023 0.0002 0 0.0003 0 0.0005 0.0008
Vacuole 0.0048 0.0052 0.0032 0.0263 0.0082 0.0041 0.0058 0.004 0.0031 0.0015 0.0047 0.002 0.0012 0.004 0.0025 0.0028 0.0012 0.001
Vacuole Periphery 0.001 0.0005 0.0004 0.0003 0.0013 0.0008 0.0032 0.0001 0.0003 0.0002 0.0009 0.0014 0.0004 0 0.0011 0 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.6747 44.412 27.6402 34.4835 58.6998 46.4503 52.3287 47.1726 39.1683 54.0615
Translational Efficiency 2.2679 1.7466 1.5696 1.3147 0.9541 1.7377 1.5159 1.0166 1.015 1.0586

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Dfg5

Dfg5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dfg5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available