Standard name
Human Ortholog
Description Component of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0.08 0.1 0.14 0.11 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.27 0 0.07 0.19 0.14 0.15 0.13 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Nucleus 0.83 0.91 0.94 0.97 0.95 0.82 0.83 0.81 0.77 0.75 0.72 0.73 0.86 0.85 0.81 0.83 0.82 0.88 0.67 0.8 0.77 0.71 0.62 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.37 0.12 0.07 0 0.05 0.19 0.13 0.21 0.28 0.24 0.3 0.35 0.14 0.12 0.1 0 0 0 0.27 0.1 0.15 0.2 0.22 0.25
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 0 2 1 3 4 2 2 7 0 0 0 1 0 0 1 2 0 0 4 3
Bud Neck 0 0 0 0 1 0 1 2 0 1 7 6 0 0 0 0 0 0 0 0 1 1 2 1
Bud Site 0 1 0 1 0 13 3 13 6 12 15 20 0 1 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 8 3 3 7 15 21 35 9 16 8 7 17 27 46 36 24 16 0 2 0 1 1 2
Endoplasmic Reticulum 1 1 0 1 0 1 0 0 0 0 0 0 1 0 0 12 8 5 0 0 0 0 1 2
Endosome 2 1 2 1 0 0 2 3 1 1 0 0 0 0 1 1 2 1 1 5 1 1 5 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 3 1 0 0 1
Mitochondria 8 42 3 4 4 110 20 44 77 67 71 81 8 1 2 13 11 13 2 8 0 1 3 7
Nucleus 287 517 227 222 337 336 379 511 317 365 344 445 187 225 261 265 191 191 240 490 197 184 204 157
Nuclear Periphery 1 3 4 1 1 2 8 5 7 2 4 4 0 0 2 2 1 2 0 1 0 0 1 0
Nucleolus 127 67 18 8 16 78 57 135 114 118 141 214 30 33 33 1 2 1 96 62 37 50 72 67
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 1 0 0 0 0
SpindlePole 1 3 7 1 5 5 13 9 2 7 9 9 2 4 6 3 2 1 3 8 3 3 11 7
Vac/Vac Membrane 2 3 2 0 2 0 5 6 3 2 2 4 0 0 1 13 14 6 8 18 9 9 16 15
Unique Cell Count 346 570 241 230 355 409 455 631 410 486 476 608 218 264 321 320 232 217 360 611 257 260 330 273
Labelled Cell Count 431 646 266 242 373 562 511 766 540 593 603 797 245 293 356 348 255 236 360 611 257 260 330 273


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.5 7.2 6.9 7.2 4.9 6.4 6.2 5.6 5.7 5.7 5.6 6.7 7.4 7.0 7.2 7.1 6.8 6.1 6.4 7.0
Std Deviation (1e-4) 1.1 1.1 1.3 1.6 1.5 1.3 1.5 1.4 1.5 1.2 1.3 1.2 1.7 1.9 1.9 1.8 1.7 1.6 1.7 1.6 1.9
Intensity Change (Log2) – – – -0.05 0.02 -0.54 -0.17 -0.2 -0.34 -0.34 -0.33 -0.35 -0.09 0.05 -0.03 0.01 -0.02 -0.07 -0.23 -0.17 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2.8 2.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.8 2.3 2.8 0 1.6 0 0 3.4 4.3 5.4 4.6 4.3 3.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 8.3 2.2 3.3 6.6 5.4 5.7 5.3 1.7 0 0 2.0 2.2 2.8
Nucleus 1.2 0.4 -4.4 -4.1 -4.8 -5.6 -6.2 -6.9 -6.8 -3.0 -3.3 -4.5 -4.1 -4.0 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.9 -1.5 4.0 2.0 4.8 6.2 5.5 6.7 8.2 2.2 1.9 1.1 -4.6 -3.6 -3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 -0.9 0 0 -1.1 -0.8 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.3 3.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3448 2.2558 2.2216 2.089 2.0852 2.1165 4.2794 3.2015 3.5568 3.3154 3.5252 3.6379 6.403 6.4944 5.9993 6.0663 5.2842 5.8693
Actin 0.0151 0 0.0226 0.005 0.0318 0.0025 0.0048 0 0 0 0.0001 0 0.0094 0 0.0001 0 0.0006 0
Bud 0.0005 0 0.0002 0.0005 0.0005 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0002 0
Bud Neck 0.0048 0 0.0015 0.0022 0.0008 0.0005 0.0008 0.0001 0 0 0.0004 0.0003 0.0005 0.0001 0.0002 0.0001 0.0015 0.0034
Bud Periphery 0.001 0 0.0004 0.0009 0.0008 0.0001 0.0003 0 0 0 0.0001 0 0.0004 0 0.0004 0 0.0009 0.0001
Bud Site 0.0132 0 0.0024 0.0028 0.0018 0.0001 0.0006 0 0 0 0.0001 0 0.0002 0.0001 0.0002 0 0.001 0.0002
Cell Periphery 0.0009 0 0.0003 0.0002 0.0001 0 0.0001 0 0 0 0 0 0.0001 0 0.0002 0 0.0002 0.0004
Cytoplasm 0.004 0.0001 0.0008 0.0015 0.0152 0.0001 0.0004 0 0 0 0 0 0.0002 0 0 0 0.0001 0
Cytoplasmic Foci 0.007 0 0.0012 0.028 0.0306 0.0011 0.0006 0 0 0 0 0 0.0016 0 0.0001 0 0.0003 0
Eisosomes 0.0003 0 0.0003 0.0001 0.0004 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0021 0 0.0017 0.0017 0.0021 0.0001 0.0002 0 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0.0003 0
Endosome 0.0181 0 0.0035 0.0295 0.0218 0.0024 0.0008 0 0 0 0.0001 0.0002 0.0011 0 0.0007 0 0.0007 0
Golgi 0.0083 0 0.002 0.0102 0.0129 0.001 0.0004 0 0 0 0 0 0.0018 0 0.0002 0 0.0002 0
Lipid Particles 0.0142 0 0.0013 0.0627 0.0466 0.005 0.0005 0 0 0 0 0 0.0076 0 0.0022 0 0.0011 0
Mitochondria 0.0046 0.0001 0.0011 0.0071 0.0147 0.0004 0.0006 0.0001 0.0001 0.0001 0.0004 0.0002 0.0005 0.0001 0.0114 0.0001 0.0079 0.0001
None 0.0049 0 0.0007 0.0013 0.0143 0.0003 0.0008 0 0 0 0.0001 0.0001 0.0004 0 0 0 0.0005 0
Nuclear Periphery 0.0225 0.0006 0.0063 0.009 0.013 0.0016 0.003 0.0004 0.0028 0.0001 0.0048 0.0009 0.0012 0.0002 0.0009 0.0001 0.0126 0.0001
Nucleolus 0.0509 0.0323 0.0184 0.0425 0.0705 0.0751 0.0363 0.0274 0.0234 0.0222 0.1984 0.0856 0.0391 0.0642 0.0372 0.0199 0.2108 0.09
Nucleus 0.8094 0.965 0.9285 0.7431 0.6702 0.9039 0.9433 0.9697 0.9732 0.9697 0.791 0.9116 0.9241 0.9003 0.9413 0.9796 0.7091 0.9002
Peroxisomes 0.0023 0 0.0004 0.0187 0.0127 0.0024 0.0002 0 0 0 0 0 0.0083 0 0.0002 0 0.0003 0
Punctate Nuclear 0.013 0.0016 0.0057 0.0299 0.033 0.0033 0.0052 0.0023 0.0002 0.0078 0.0042 0.0005 0.002 0.035 0.0011 0.0002 0.0493 0.0042
Vacuole 0.0015 0 0.0005 0.0021 0.0039 0.0001 0.0005 0 0 0 0.0001 0.0001 0.001 0 0.002 0 0.001 0.0012
Vacuole Periphery 0.0018 0 0.0003 0.0011 0.0021 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0012 0 0.0014 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.8496 24.4153 20.5328 16.6036 25.9682 16.3781 25.0977 27.2139 22.0329 18.6829
Translational Efficiency 1.9102 2.0703 1.5574 1.6486 1.441 2.4426 1.6841 1.4415 1.5432 1.9993

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1864 1417 483 1869 2026 2411 2183 1178 3890 3828 2666 3047

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 757.25 830.69 939.62 1045.45 764.57 891.81 975.55 1055.91 761.06 869.19 969.04 1049.49
Standard Deviation 110.50 146.94 166.08 173.25 101.48 128.26 140.50 166.35 105.96 138.66 146.13 170.69
Intensity Change Log 2 — 0.133541 0.311308 0.465282 — 0.222088 0.351567 0.465766 — 0.178706 0.331675 0.465526

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000350 0.000735 0.001173 0.000969 0.000183 0.000622 0.000525 0.001031 0.000263 0.000664 0.000642 0.000993
Bud Neck 0.005102 0.011220 0.013773 0.023998 0.005769 0.010465 0.021481 0.029598 0.005450 0.010745 0.020084 0.026163
Bud Site 0.000501 0.001589 0.001259 0.001678 0.000439 0.001590 0.000901 0.005823 0.000469 0.001590 0.000966 0.003281
Cell Periphery 0.000071 0.000152 0.000044 0.000077 0.000067 0.000082 0.000029 0.000120 0.000069 0.000108 0.000032 0.000093
Cytoplasm 0.002828 0.005591 0.000429 0.001151 0.001900 0.001387 0.000127 0.002875 0.002345 0.002943 0.000182 0.001818
Cytoplasmic Foci 0.000520 0.000946 0.000040 0.000228 0.000235 0.000819 0.000007 0.000432 0.000371 0.000866 0.000013 0.000307
Eisosomes 0.000025 0.000040 0.000042 0.000041 0.000020 0.000041 0.000044 0.000043 0.000023 0.000040 0.000044 0.000042
Endoplasmic Reticulum 0.002063 0.003730 0.002731 0.002081 0.001393 0.002428 0.001361 0.003337 0.001714 0.002910 0.001609 0.002567
Endosome 0.000270 0.000376 0.000108 0.000406 0.000173 0.000927 0.000049 0.000765 0.000220 0.000723 0.000060 0.000545
Golgi 0.000321 0.000303 0.000187 0.000160 0.000085 0.000438 0.000095 0.000463 0.000198 0.000388 0.000112 0.000277
Lipid Particles 0.000696 0.000196 0.000070 0.000142 0.000068 0.000632 0.000012 0.000098 0.000369 0.000470 0.000022 0.000125
Mitochondria 0.002207 0.001347 0.001186 0.001903 0.000818 0.002358 0.000751 0.002640 0.001484 0.001984 0.000830 0.002188
Mitotic Spindle 0.001791 0.002244 0.001748 0.003415 0.000366 0.005019 0.000254 0.018975 0.001049 0.003992 0.000525 0.009430
None 0.007116 0.009275 0.001797 0.002624 0.004460 0.003882 0.000925 0.003945 0.005733 0.005878 0.001083 0.003135
Nuclear Periphery 0.000297 0.000194 0.000127 0.000638 0.000216 0.000376 0.000080 0.000866 0.000255 0.000308 0.000089 0.000726
Nuclear Periphery Foci 0.000586 0.000670 0.000267 0.000359 0.000288 0.000850 0.000038 0.000903 0.000431 0.000783 0.000080 0.000570
Nucleolus 0.086057 0.098915 0.079318 0.048844 0.083158 0.089461 0.071736 0.035939 0.084547 0.092961 0.073109 0.043855
Nucleus 0.887984* 0.860749* 0.893665* 0.908716* 0.899779* 0.876161* 0.900899* 0.886815* 0.894127* 0.870456* 0.899589* 0.900249*
Peroxisomes 0.000927 0.000941 0.001359 0.000494 0.000250 0.000733 0.000422 0.000501 0.000574 0.000810 0.000592 0.000497
Vacuole 0.000158 0.000431 0.000470 0.000968 0.000145 0.001137 0.000131 0.002911 0.000151 0.000876 0.000192 0.001719
Vacuole Periphery 0.000128 0.000356 0.000207 0.001107 0.000188 0.000592 0.000135 0.001920 0.000159 0.000505 0.000148 0.001421

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.01 -9.92 -3.83 -1.58 1.44 -2.03 -23.70 -20.14 -3.24 -13.79 -2.89 -14.96 -8.15 -2.77 -4.33
Bud Neck -7.24 -8.76 -18.61 -11.27 -6.55 -7.25 -20.82 -17.65 -14.36 -6.46 -10.47 -23.50 -25.44 -18.48 -6.98
Bud Site -4.62 -6.88 -6.30 -0.78 -1.43 -2.96 -3.22 -5.85 -4.45 -5.43 -4.56 -6.42 -7.62 -4.17 -6.26
Cell Periphery -4.24 2.21 -0.21 3.64 -1.94 -0.92 2.97 -2.26 -1.69 -4.84 -3.29 4.32 -1.61 1.25 -5.24
Cytoplasm -2.10 3.40 1.73 3.55 -1.89 0.96 3.50 -1.66 -2.60 -4.71 -0.81 5.04 0.41 1.24 -4.83
Cytoplasmic Foci -0.93 2.69 1.91 2.07 -1.52 -1.79 2.58 0.18 1.94 -2.76 -1.76 3.71 1.76 2.94 -3.04
Eisosomes -6.85 -7.96 -8.14 -0.20 1.41 -13.06 -17.40 -16.02 -2.75 -1.44 -13.81 -17.52 -16.03 -1.48 0.66
Endoplasmic Reticulum -7.18 -3.43 -0.26 6.57 2.96 -7.89 0.39 -8.75 -5.01 -9.09 -9.73 1.21 -6.92 0.52 -8.08
Endosome -1.29 2.77 -0.47 0.23 -1.65 -3.68 5.40 -3.13 1.20 -4.16 -3.77 5.42 -2.11 1.62 -3.83
Golgi 0.12 1.03 1.29 2.82 1.25 -2.99 -1.14 -3.14 0.40 -3.03 -2.02 1.44 -0.71 1.67 -3.38
Lipid Particles 1.82 2.33 2.10 1.01 -0.60 -2.09 5.96 -2.66 1.81 -5.40 -0.48 2.75 1.79 1.96 -2.45
Mitochondria 1.32 1.38 0.25 -3.17 -3.56 -3.14 0.07 -4.56 -1.06 -7.36 -1.19 1.67 -2.21 -1.32 -9.08
Mitotic Spindle -0.55 -0.44 -3.04 -2.31 -2.28 -4.60 -0.45 -6.76 -5.26 -6.74 -3.75 0.72 -7.48 -5.25 -7.72
None -1.42 5.45 4.08 5.21 -1.51 0.69 6.23 0.21 -0.52 -6.60 -0.19 8.44 3.69 3.83 -5.60
Nuclear Periphery 1.58 2.34 -2.65 -3.52 -3.85 -2.20 2.91 -5.69 -4.52 -6.87 -1.02 4.12 -5.32 -4.87 -7.10
Nuclear Periphery Foci -0.67 2.25 1.07 1.51 -0.94 -1.82 3.30 -1.86 0.00 -3.09 -1.73 4.39 -0.60 1.15 -3.64
Nucleolus -3.17 -0.16 9.98 12.52 6.13 -1.62 2.51 13.38 15.97 11.27 -3.04 2.76 15.64 18.86 11.17
Nucleus 5.19 0.76 -2.41 -7.42 -2.51 5.08 0.70 4.18 0.61 3.87 6.76 -0.08 1.22 -4.65 1.27
Peroxisomes 0.02 -1.69 1.51 4.09 6.58 -3.31 -3.53 -4.66 1.10 -2.58 -1.26 -0.20 0.45 2.89 2.15
Vacuole -3.26 -2.94 -4.00 -2.62 -1.35 -2.88 0.86 -4.24 -3.09 -4.43 -3.51 -0.16 -5.29 -3.15 -5.35
Vacuole Periphery -8.72 -4.31 -1.95 -1.39 -1.72 -3.22 1.46 -1.70 -1.01 -1.79 -4.37 0.34 -2.56 -1.51 -2.58
Standard name
Human Ortholog
Description Component of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sap30

Sap30


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sap30-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available