Standard name
Human Ortholog
Description Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.07 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.05 – 0 0.15 0.1 0.13 – 0.06 0.08 0.11 0.07 – – – 0.07 0.07 0 0.06 0 0.05 0 0.15 0.24
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0.21 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.39 0 – 0.08 0 0.2 0.29 – 0.45 0.56 0.7 0.58 – – – 0 0 0 0.05 0 0.05 0 0 0
Nucleus 0.6 0.73 – 0.62 0.62 0.66 0.61 – 0.73 0.68 0.48 0.64 – – – 0.83 0.84 0.84 0.51 0.71 0.43 0 0.41 0.29
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.1 0.28 – 0.08 0.19 0.15 0.08 – 0.06 0.05 0.05 0 – – – 0.1 0.1 0.12 0.16 0.12 0.29 0 0.3 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 1 0 0 0 0
Bud 0 0 – 0 0 1 0 – 1 1 3 2 – – – 0 2 0 2 5 1 0 0 0
Bud Neck 0 0 – 0 2 1 1 – 0 1 1 0 – – – 0 0 0 1 0 0 0 0 0
Bud Site 1 0 – 0 0 0 0 – 0 1 2 3 – – – 0 0 1 – – – – – –
Cell Periphery 0 1 – 0 0 0 0 – 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Cytoplasm 17 12 – 1 11 12 13 – 3 9 14 7 – – – 26 22 14 11 4 2 0 1 1
Endoplasmic Reticulum 0 1 – 0 0 0 0 – 0 0 0 1 – – – 8 10 8 1 0 0 0 0 0
Endosome 2 2 – 5 1 1 0 – 0 0 0 0 – – – 0 1 5 9 7 1 0 0 0
Golgi 0 1 – 0 0 0 0 – 0 0 0 0 – – – 1 1 3 0 2 0 0 0 0
Mitochondria 69 4 – 2 2 23 30 – 22 61 88 60 – – – 11 4 1 9 5 2 0 0 0
Nucleus 105 182 – 15 46 76 62 – 36 73 60 67 – – – 294 280 267 106 195 23 0 4 1
Nuclear Periphery 1 1 – 0 0 0 0 – 0 0 1 2 – – – 1 3 1 6 2 3 0 0 0
Nucleolus 3 0 – 1 0 0 1 – 0 0 0 1 – – – 1 1 1 5 6 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
SpindlePole 0 3 – 0 1 1 0 – 1 0 0 0 – – – 1 0 0 11 6 1 0 0 0
Vac/Vac Membrane 17 70 – 2 14 17 8 – 3 5 6 4 – – – 37 33 37 34 32 15 0 3 0
Unique Cell Count 176 248 24 74 116 102 49 108 126 104 353 332 319 209 275 54 1 12 6
Labelled Cell Count 215 277 26 77 132 115 66 151 175 147 381 358 338 209 275 54 1 12 6


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.6 – 5.9 6.1 4.9 5.1 – 3.7 3.9 3.7 3.6 – – – 8.5 8.5 8.6 – – –
Std Deviation (1e-4) 0.7 1.0 – 2.0 1.9 1.4 1.9 – 0.4 0.8 0.8 0.7 – – – 1.7 1.8 1.8 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6963 0.0097 0.1652 -0.2379 0.2103 0.0248 -0.2858 -0.1569 -0.2876 -0.3093 0.0063 -0.1873 -0.1955 -0.1519 -0.5975 -0.5789 -0.802 -0.2773
Actin 0.04 0.0004 0.0159 0.004 0.0121 0.0031 0.0186 0.0007 0.0234 0.0106 0.0311 0.0146 0.0229 0.0003 0.0051 0.0229 0.0003 0.0109
Bud 0.0006 0.0001 0.0002 0.0001 0.0022 0.0001 0.0003 0.0003 0.0006 0.0003 0.0002 0.0002 0.0099 0.0001 0.0035 0.0002 0 0.0029
Bud Neck 0.0061 0.0006 0.0012 0.0005 0.0046 0.002 0.0049 0.0007 0.0107 0.0004 0.0015 0.0032 0.0023 0.001 0.002 0.0011 0.0008 0.0043
Bud Periphery 0.0013 0.0001 0.0002 0.0002 0.0047 0.0002 0.0004 0.0008 0.0018 0.0008 0.0002 0.0005 0.0046 0.0001 0.0033 0.0002 0 0.0016
Bud Site 0.003 0.0004 0.0017 0.0005 0.0323 0.0002 0.0155 0.0068 0.0084 0.0005 0.0006 0.0003 0.003 0.0012 0.0033 0.0005 0.0002 0.0013
Cell Periphery 0.0003 0 0.0001 0.0001 0.0016 0.0001 0.0005 0.0004 0.0007 0.0001 0.0001 0.0001 0.0005 0.0001 0.0002 0 0 0.0002
Cytoplasm 0.0527 0.0742 0.041 0.0257 0.0457 0.0305 0.0457 0.0758 0.0616 0.0333 0.0203 0.0745 0.0628 0.1265 0.064 0.0677 0.1338 0.0455
Cytoplasmic Foci 0.0163 0.0034 0.0094 0.002 0.0076 0.0043 0.0252 0.0036 0.0076 0.0024 0.0217 0.0023 0.0226 0.0021 0.0089 0.0026 0.0035 0.0064
Eisosomes 0.0006 0.0001 0.0003 0.0001 0.0003 0.0001 0.0004 0 0.0002 0.0006 0.0002 0.0002 0.0003 0 0.0002 0.0003 0 0.0001
Endoplasmic Reticulum 0.0101 0.0041 0.004 0.0015 0.004 0.0016 0.0057 0.0066 0.0055 0.0013 0.0013 0.0072 0.0051 0.005 0.0036 0.0019 0.0018 0.0032
Endosome 0.0397 0.0041 0.0053 0.0045 0.023 0.0034 0.0086 0.0039 0.009 0.0081 0.0443 0.016 0.0316 0.0018 0.0072 0.0024 0.0024 0.0059
Golgi 0.0119 0.0001 0.0012 0.0015 0.0032 0.0015 0.0039 0.0005 0.0022 0.0039 0.0532 0.003 0.0102 0.0001 0.0024 0.0033 0 0.0031
Lipid Particles 0.0222 0.0006 0.004 0.0089 0.0053 0.0053 0.0114 0.0023 0.0105 0.0581 0.0203 0.002 0.0189 0.0002 0.0061 0.0071 0.0001 0.0042
Mitochondria 0.0139 0.0006 0.0015 0.0102 0.0585 0.0041 0.0026 0.0013 0.0021 0.0684 0.0078 0.0139 0.005 0.0005 0.002 0.0149 0.0004 0.0023
None 0.0711 0.1315 0.0781 0.0693 0.0165 0.0564 0.0902 0.1293 0.1246 0.0536 0.0783 0.1182 0.1017 0.1294 0.101 0.0454 0.1262 0.0185
Nuclear Periphery 0.0845 0.0745 0.1081 0.08 0.0338 0.0373 0.068 0.0448 0.0855 0.0158 0.0221 0.036 0.0662 0.0373 0.0502 0.0334 0.0658 0.0262
Nucleolus 0.0064 0.0053 0.0067 0.0063 0.0181 0.0108 0.0042 0.0082 0.0057 0.0027 0.0055 0.0099 0.0046 0.006 0.0047 0.0032 0.0064 0.0146
Nucleus 0.5589 0.6374 0.6557 0.7527 0.675 0.7748 0.6426 0.6746 0.5994 0.642 0.6385 0.6371 0.5801 0.626 0.6865 0.7043 0.6179 0.8039
Peroxisomes 0.0151 0.0001 0.0042 0.0056 0.0052 0.0019 0.0174 0.0017 0.0033 0.0037 0.0322 0.001 0.0143 0.0001 0.0026 0.0081 0 0.0025
Punctate Nuclear 0.0346 0.0599 0.0591 0.0245 0.0288 0.0606 0.0302 0.0338 0.033 0.0901 0.018 0.056 0.0269 0.0601 0.0407 0.0793 0.0388 0.039
Vacuole 0.0061 0.0018 0.001 0.0011 0.016 0.0012 0.0025 0.0033 0.0028 0.0012 0.0012 0.0025 0.0048 0.0019 0.0017 0.0007 0.001 0.0027
Vacuole Periphery 0.005 0.0007 0.0011 0.0008 0.0015 0.0006 0.0014 0.0008 0.0014 0.002 0.0011 0.0014 0.002 0.0005 0.0008 0.0004 0.0005 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1846 1.6358 3.2369 8.987 5.1404 7.925 6.6157 9.4885 9.3232 8.8853
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
233 1433 281 1528 1813 1800 231 201 2046 3233 512 1729

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 682.75 728.23 1073.44 831.40 666.89 735.30 987.99 906.75 668.70 732.17 1034.89 840.16
Standard Deviation 68.80 94.79 123.10 124.09 86.16 93.55 116.18 119.71 84.52 94.17 127.34 125.92
Intensity Change Log 2 — 0.093037 0.652812 0.284185 — 0.140884 0.567048 0.443256 — 0.116878 0.611071 0.364978

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.000410 0.001789 0.000763 0.000135 0.000133 0.001243 0.001626 0.000135 0.000256 0.001543 0.000863
Bud Neck 0.000835 0.006762 0.005746 0.012280 0.002613 0.005281 0.006608 0.007085 0.002410 0.005937 0.006135 0.011676
Bud Site 0.000163 0.001741 0.001539 0.009779 0.000399 0.001143 0.002036 0.014830 0.000372 0.001408 0.001763 0.010366
Cell Periphery 0.000141 0.000104 0.000094 0.000259 0.000083 0.000085 0.000100 0.000232 0.000090 0.000094 0.000097 0.000256
Cytoplasm 0.146282* 0.115415* 0.003715 0.122295* 0.136325* 0.118973* 0.015297 0.093932 0.137459* 0.117396* 0.008941 0.118997*
Cytoplasmic Foci 0.054386 0.028967 0.000241 0.001994 0.018699 0.020535 0.000393 0.007091 0.022763 0.024272 0.000310 0.002587
Eisosomes 0.000074 0.000047 0.000061 0.000025 0.000033 0.000032 0.000060 0.000048 0.000037 0.000039 0.000060 0.000028
Endoplasmic Reticulum 0.002069 0.005336 0.018419 0.007954 0.001939 0.003184 0.020213 0.009608 0.001954 0.004138 0.019228 0.008147
Endosome 0.002323 0.002839 0.000762 0.001303 0.001440 0.003612 0.001020 0.002239 0.001541 0.003270 0.000878 0.001412
Golgi 0.000379 0.001546 0.000110 0.000270 0.000268 0.000915 0.000083 0.013348 0.000281 0.001195 0.000098 0.001790
Lipid Particles 0.020356 0.008574 0.000972 0.000587 0.006170 0.007727 0.000989 0.000992 0.007785 0.008102 0.000980 0.000634
Mitochondria 0.004834 0.009030 0.001340 0.003114 0.002081 0.008163 0.000971 0.001992 0.002395 0.008548 0.001173 0.002983
Mitotic Spindle 0.000102 0.008796 0.000128 0.014227 0.001834 0.004723 0.000662 0.031289 0.001637 0.006529 0.000369 0.016210
None 0.020051 0.014998 0.001152 0.009907 0.019368 0.009621 0.006745 0.025530 0.019446 0.012004 0.003675 0.011723
Nuclear Periphery 0.004214 0.008051 0.001885 0.004378 0.005044 0.009646 0.003714 0.002735 0.004950 0.008939 0.002710 0.004187
Nuclear Periphery Foci 0.005175 0.006444 0.000941 0.001709 0.003136 0.003124 0.001967 0.001402 0.003368 0.004595 0.001404 0.001673
Nucleolus 0.022957 0.020856 0.004183 0.002142 0.016579 0.015863 0.003838 0.001356 0.017305 0.018076 0.004027 0.002051
Nucleus 0.707429* 0.749007* 0.941265* 0.788412* 0.779354* 0.776580* 0.915084* 0.755133* 0.771163* 0.764358* 0.929453* 0.784543*
Peroxisomes 0.001972 0.004040 0.000403 0.000762 0.001624 0.002964 0.000353 0.000206 0.001664 0.003441 0.000380 0.000697
Vacuole 0.005508 0.006056 0.015015 0.016944 0.002657 0.006989 0.018408 0.028771 0.002982 0.006575 0.016546 0.018319
Vacuole Periphery 0.000613 0.000982 0.000242 0.000898 0.000220 0.000707 0.000215 0.000556 0.000265 0.000829 0.000230 0.000858

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.77 -11.77 -6.20 -4.00 7.09 -0.17 -10.89 -6.76 -6.77 0.15 -4.53 -16.61 -9.24 -7.63 7.00
Bud Neck -7.87 -8.75 -13.58 -6.91 -6.12 -3.74 -4.17 -6.53 -4.12 -0.99 -5.59 -6.59 -11.76 -8.72 -6.19
Bud Site -6.88 -6.71 -6.90 -5.40 -4.68 -4.09 -4.31 -2.58 -2.37 -1.94 -6.96 -7.16 -7.18 -6.21 -5.05
Cell Periphery 1.62 1.11 -1.99 -4.37 -3.64 -0.15 -2.90 -5.36 -5.24 -2.81 -0.68 -3.01 -5.69 -5.51 -4.02
Cytoplasm 2.39 11.60 4.45 4.41 -22.84 2.81 24.60 9.15 7.38 -6.40 3.87 29.81 9.58 7.05 -22.04
Cytoplasmic Foci 4.13 9.30 9.10 13.57 -4.12 -0.68 14.70 10.55 10.52 -1.75 -0.48 17.15 16.05 18.59 -4.33
Eisosomes 3.58 0.69 6.20 5.35 6.25 1.00 -5.54 -6.72 -7.10 -1.01 -0.49 -6.96 -0.41 0.08 6.85
Endoplasmic Reticulum -4.98 -13.59 -13.38 -10.56 6.17 -4.40 -11.57 -10.80 -9.75 2.90 -8.61 -18.29 -22.15 -16.34 7.71
Endosome -0.79 2.06 1.69 4.63 -1.55 -2.98 0.98 -2.02 1.70 -2.93 -3.72 2.31 1.29 4.84 -1.69
Golgi -2.78 1.37 1.20 3.72 -0.51 -1.91 4.95 -0.93 -0.66 -1.01 -3.56 4.70 -0.43 2.00 -1.12
Lipid Particles 3.04 5.16 5.26 9.92 2.70 -1.56 7.66 7.35 8.56 -0.92 -0.38 9.31 9.81 13.63 2.70
Mitochondria -1.35 1.02 0.65 5.77 -2.18 -6.60 4.68 0.70 6.54 -1.77 -7.97 2.72 -0.16 7.55 -2.59
Mitotic Spindle -5.22 -1.68 -6.60 -1.26 -6.45 -2.59 1.09 -2.64 -2.10 -2.79 -4.67 1.61 -6.08 -3.04 -6.89
None 1.44 5.92 3.98 6.39 -13.47 5.72 6.67 1.74 -1.32 -2.96 4.79 10.92 8.00 4.96 -5.85
Nuclear Periphery -3.53 2.27 -2.22 2.29 -9.53 -4.48 0.43 -0.77 3.99 -1.06 -5.16 2.44 -1.91 4.25 -5.44
Nuclear Periphery Foci -1.47 1.99 2.66 5.73 0.23 0.13 2.12 2.21 1.86 -0.01 -2.26 2.28 3.92 5.97 0.34
Nucleolus 0.14 4.58 5.18 13.15 3.52 1.59 9.38 11.15 10.80 1.64 0.29 10.73 12.70 17.21 3.74
Nucleus -1.98 -11.60 -5.38 -6.88 14.38 0.06 -12.83 0.51 0.48 7.35 0.93 -19.51 -4.25 -5.68 15.70
Peroxisomes -3.04 2.87 2.78 7.49 0.03 -2.43 4.14 4.52 6.54 1.95 -4.11 4.57 4.16 9.09 -0.04
Vacuole -0.88 -5.83 -11.44 -11.99 -2.63 -3.98 -7.16 -7.61 -7.21 -4.88 -5.30 -9.79 -15.32 -13.63 -4.12
Vacuole Periphery -0.86 0.81 0.27 1.89 -1.03 -4.65 0.07 -0.75 3.22 -0.73 -5.26 0.19 -1.11 1.89 -1.20
Standard name
Human Ortholog
Description Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Yku70

Yku70


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yku70-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available