Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.91 0.74 0.72 0.83 0.65 0.64 0.66 0.65 0.68 0.63 0.94 0.98 0.93 1.0 – – 0.9 0.84 0.76 0.7 0.78 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.07 0.1 0.12 0.1 0.13 0 0 0 0 – – 0.07 0.11 0.12 0.21 0.09 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0.09 0.43 0.37 0.18 0.29 0.31 0.27 0.24 0.16 0.23 0.11 0.08 0.13 0 – – 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 2 2 1 1 2 0 0 0 0 – – 0 1 0 0 0 1
Bud 0 1 0 0 1 0 1 6 0 2 4 0 0 0 0 0 – – 0 2 1 1 7 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 2 0 1 0 2 3 2 1 3 2 7 0 2 0 0 – – 0 0 0 0 0 0
Cytoplasm 197 366 31 144 257 237 336 340 232 265 226 251 116 216 288 19 – – 195 316 56 49 124 195
Endoplasmic Reticulum 1 8 0 0 3 6 18 7 5 6 6 4 2 3 1 2 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 1 4 0 0 1 0 0 0 0 0 – – 0 0 0 1 1 1
Golgi 0 0 0 0 1 3 3 2 1 1 2 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 11 3 10 4 25 23 0 1 0 0 – – 0 1 0 0 0 2
Nucleus 2 8 0 4 7 11 23 39 36 50 32 53 1 1 5 0 – – 14 43 9 15 14 18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 0
Vac/Vac Membrane 12 11 3 84 132 53 150 165 96 99 55 92 14 18 40 0 – – 2 9 3 2 5 2
Unique Cell Count 210 381 34 195 357 287 520 530 353 405 334 400 124 221 310 19 217 379 74 71 160 236
Labelled Cell Count 212 397 34 233 401 313 547 570 383 431 355 432 133 241 334 21 217 379 74 71 160 236


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.6 22.5 14.6 18.1 16.9 14.3 12.9 12.8 11.0 11.1 10.4 9.6 26.3 26.2 23.9 29.3 – – 22.8 23.1 22.0
Std Deviation (1e-4) 2.6 4.7 3.1 3.5 2.7 2.2 2.0 2.0 2.0 1.9 2.1 1.9 5.5 6.8 6.2 6.2 – – 5.4 5.1 5.4
Intensity Change (Log2) – – – 0.31 0.21 -0.04 -0.18 -0.2 -0.4 -0.4 -0.49 -0.6 0.85 0.84 0.71 1.0 – – 0.64 0.66 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Cytoplasm -2.2 -2.4 -1.3 -3.2 -3.2 -3.0 -3.1 -2.8 -3.3 0.5 2.0 0.4 1.3 – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – –
Vacuole 3.8 3.3 1.4 2.5 2.8 2.3 2.1 1.2 1.9 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.8914 27.1098 24.2048 20.3364 20.3677 25.3434 17.6584 23.3962 22.5714 23.0587 17.7556 24.1602 19.7678 17.1944 17.4396 21.4442 21.2405 18.6303
Actin 0.0019 0.0004 0.0056 0.031 0.0071 0.0001 0.0088 0.0006 0.0077 0.0002 0.111 0.0048 0.0001 0.0042 0.0295 0.0002 0.0002 0.0003
Bud 0.0009 0.0003 0.0008 0.0021 0.0015 0.002 0.0036 0.0001 0.0013 0.0002 0.0027 0.0003 0.0001 0.0003 0.0006 0.0001 0.0001 0.0001
Bud Neck 0.0008 0.0004 0.0016 0.0032 0.0041 0.001 0.0012 0.0004 0.0017 0.0007 0.0012 0.001 0.0002 0.0003 0.0011 0.0002 0.0005 0.0005
Bud Periphery 0.0011 0 0.001 0.0022 0.0015 0.0006 0.0026 0 0.0008 0.0001 0.0057 0.0002 0 0.0001 0.0005 0 0 0
Bud Site 0.0014 0.0001 0.0016 0.0025 0.001 0.0001 0.0053 0.0002 0.008 0.0009 0.0125 0.0001 0 0.0011 0.0013 0 0 0
Cell Periphery 0.0004 0.0001 0.0004 0.0005 0.0004 0.0001 0.0003 0.0001 0.0002 0.0001 0.0016 0 0 0.0001 0.0002 0 0 0
Cytoplasm 0.3968 0.5308 0.394 0.3398 0.3893 0.3757 0.4568 0.7592 0.621 0.4442 0.3819 0.5642 0.4353 0.7718 0.3933 0.4151 0.8341 0.5748
Cytoplasmic Foci 0.0048 0.0006 0.007 0.0161 0.0197 0.0005 0.0188 0.0005 0.0172 0.0048 0.0099 0.0005 0.0001 0.0028 0.0019 0.0001 0.0002 0.0015
Eisosomes 0 0 0.0001 0.0007 0.0001 0 0.0001 0 0.0001 0 0.0003 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0039 0.0007 0.0072 0.0018 0.0097 0.0016 0.0047 0.0017 0.0013 0.0004 0.0187 0.0016 0.0005 0.0017 0.0026 0.0001 0.0007 0.0027
Endosome 0.0063 0.0001 0.0126 0.027 0.0244 0.0022 0.0386 0.0007 0.0147 0.0298 0.0406 0.0047 0.0001 0.0019 0.0131 0 0.0002 0.0034
Golgi 0.003 0 0.0025 0.0146 0.0205 0 0.0134 0.0001 0.0053 0.0024 0.0055 0.0049 0 0.0003 0.015 0 0 0.0001
Lipid Particles 0.0025 0.0001 0.0029 0.005 0.0135 0.0001 0.0255 0.0001 0.0038 0.0029 0.0132 0.0043 0 0.0001 0.0012 0 0 0.0001
Mitochondria 0.0123 0.0002 0.0085 0.0499 0.0222 0.0008 0.0136 0.0002 0.0034 0.001 0.0071 0.0018 0.0003 0.0004 0.0035 0.0003 0.0001 0.0002
None 0.0045 0.0013 0.0016 0.0004 0.0045 0.0002 0.0066 0.0008 0.004 0.0002 0.0166 0.0003 0.0003 0.0011 0.0004 0.0001 0.0002 0.0008
Nuclear Periphery 0.0448 0.0247 0.0297 0.0201 0.0545 0.0333 0.0282 0.0113 0.0161 0.0149 0.0139 0.03 0.0223 0.0178 0.0186 0.0153 0.0217 0.0277
Nucleolus 0.0021 0.0003 0.0008 0.0004 0.0034 0.0006 0.0061 0.0001 0.0007 0.0007 0.0012 0.0002 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002
Nucleus 0.4934 0.4377 0.5097 0.4661 0.3622 0.5771 0.3278 0.2226 0.283 0.4726 0.2959 0.377 0.5386 0.1918 0.5138 0.5666 0.1398 0.3809
Peroxisomes 0.0004 0 0.0016 0.0041 0.004 0 0.006 0 0.0033 0.0006 0.0013 0.0002 0 0.0002 0.0002 0 0 0
Punctate Nuclear 0.0074 0.0014 0.0024 0.0008 0.0232 0.0011 0.0232 0.0008 0.0029 0.0011 0.0015 0.002 0.0009 0.0011 0.0014 0.0013 0.0017 0.0056
Vacuole 0.0086 0.0007 0.006 0.0086 0.0273 0.0025 0.0067 0.0005 0.0029 0.0199 0.0486 0.0011 0.0006 0.0021 0.0009 0.0001 0.0003 0.0006
Vacuole Periphery 0.0027 0.0001 0.0023 0.0029 0.0058 0.0007 0.0022 0.0001 0.0006 0.0024 0.0093 0.0009 0.0002 0.0005 0.0006 0.0001 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.098 14.5632 19.5349 18.6047 12.9982 28.1804 40.6349 43.3844 41.9006 25.9501
Translational Efficiency 1.6158 1.5205 0.8566 0.9224 0.9117 1.4365 1.346 1.1805 1.1974 1.1296

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
864 1829 189 242 532 1930 129 292 1396 3759 318 534

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1191.70 1354.28 1684.45 1480.71 1503.08 1320.36 1794.91 1748.97 1310.36 1336.86 1729.26 1627.40
Standard Deviation 174.61 256.53 357.50 320.51 255.19 242.88 252.03 334.22 257.98 250.19 323.53 354.21
Intensity Change Log 2 — 0.184505 0.499257 0.313268 — -0.186990 0.255990 0.218584 — -0.010823 0.368654 0.261224

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000460 0.000293 0.001255 0.000709 0.000496 0.000490 0.000971 0.000361 0.000474 0.000394 0.001140 0.000519
Bud Neck 0.001323 0.003922 0.002829 0.004099 0.002476 0.003208 0.004748 0.003044 0.001762 0.003555 0.003607 0.003522
Bud Site 0.002031 0.002492 0.002940 0.014394 0.002492 0.003468 0.006438 0.015876 0.002207 0.002993 0.004359 0.015205
Cell Periphery 0.000145 0.000110 0.000345 0.000155 0.000188 0.000217 0.000397 0.000214 0.000161 0.000165 0.000366 0.000188
Cytoplasm 0.397591* 0.286487* 0.213725* 0.429571* 0.243135* 0.174256* 0.267370* 0.539791* 0.338730* 0.228864* 0.235487* 0.489841*
Cytoplasmic Foci 0.007514 0.002308 0.004674 0.031563 0.004732 0.002364 0.008817 0.013772 0.006453 0.002337 0.006355 0.021834
Eisosomes 0.000009 0.000005 0.000014 0.000013 0.000008 0.000008 0.000022 0.000009 0.000009 0.000007 0.000018 0.000011
Endoplasmic Reticulum 0.002511 0.001174 0.002473 0.001481 0.002037 0.001655 0.004816 0.000430 0.002330 0.001421 0.003424 0.000906
Endosome 0.001136 0.000469 0.000539 0.018545 0.000904 0.001445 0.003626 0.041010 0.001048 0.000970 0.001791 0.030829
Golgi 0.000304 0.000480 0.000271 0.010906 0.000962 0.001389 0.000423 0.002058 0.000555 0.000947 0.000333 0.006068
Lipid Particles 0.000485 0.000265 0.000723 0.002493 0.000363 0.000431 0.002520 0.000883 0.000438 0.000350 0.001452 0.001613
Mitochondria 0.000407 0.000726 0.000780 0.035083 0.000508 0.002343 0.000809 0.000522 0.000445 0.001557 0.000791 0.016185
Mitotic Spindle 0.003970 0.004386 0.001231 0.043488 0.003984 0.008800 0.003296 0.019069 0.003975 0.006653 0.002069 0.030136
None 0.007983 0.009127 0.004664 0.011481 0.006485 0.008486 0.003558 0.020672 0.007412 0.008798 0.004216 0.016507
Nuclear Periphery 0.000392 0.000408 0.000741 0.003617 0.000380 0.000580 0.001607 0.000551 0.000388 0.000496 0.001092 0.001940
Nuclear Periphery Foci 0.003580 0.001036 0.002313 0.012225 0.001606 0.000840 0.008121 0.001242 0.002828 0.000936 0.004669 0.006219
Nucleolus 0.000376 0.000932 0.000199 0.000183 0.000801 0.000462 0.000198 0.000183 0.000538 0.000691 0.000199 0.000183
Nucleus 0.559371* 0.679099* 0.728022* 0.305322* 0.719239* 0.781831* 0.590168* 0.249122* 0.620295* 0.731845* 0.672100* 0.274591*
Peroxisomes 0.000409 0.000116 0.000080 0.000609 0.000144 0.000538 0.000142 0.000374 0.000308 0.000333 0.000105 0.000480
Vacuole 0.009798 0.005933 0.032068 0.058254 0.008505 0.006710 0.091653 0.075035 0.009305 0.006332 0.056239 0.067430
Vacuole Periphery 0.000207 0.000229 0.000113 0.015806 0.000554 0.000479 0.000301 0.015783 0.000339 0.000358 0.000189 0.015793

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.64 -6.05 -1.30 -2.77 4.81 0.44 -6.20 2.35 1.39 7.46 1.12 -8.26 -0.00 -1.09 8.19
Bud Neck -5.25 -5.08 -4.80 -0.78 1.47 -1.60 -4.24 -3.84 -2.33 2.11 -5.65 -6.60 -6.97 -1.91 2.38
Bud Site -0.85 -3.61 -2.43 -2.26 -1.41 -0.87 -2.86 -2.97 -2.57 -0.60 -1.35 -4.37 -3.88 -3.43 -1.64
Cell Periphery 2.01 -6.52 -3.52 -5.03 5.06 0.19 -7.59 -3.79 -1.60 4.64 0.36 -9.43 -5.48 -2.77 6.36
Cytoplasm 11.28 14.37 0.94 -6.15 -10.29 6.94 0.86 -8.62 -15.10 -7.87 14.83 11.88 -3.73 -14.81 -13.27
Cytoplasmic Foci 4.78 1.20 -1.12 -3.57 -1.85 2.27 -2.38 -4.28 -7.43 -1.88 5.29 -0.31 -3.16 -7.16 -2.86
Eisosomes 3.69 -4.89 -5.40 -7.93 0.41 -0.21 -6.54 -5.45 -7.11 4.09 2.25 -8.00 -7.70 -10.73 3.19
Endoplasmic Reticulum 5.45 -0.02 6.38 2.14 3.79 1.10 -2.13 7.28 13.23 4.80 4.65 -1.55 10.20 9.70 6.35
Endosome 2.03 0.91 -1.44 -2.08 -1.76 -0.58 -3.07 -0.85 -0.67 0.23 0.79 -1.60 -1.44 -1.67 -0.94
Golgi -0.56 -1.26 -1.44 -1.21 -1.32 -0.17 1.28 1.68 1.95 1.75 -0.75 0.80 -0.70 -0.17 -1.10
Lipid Particles 1.59 -4.22 -5.61 -6.43 -1.03 -0.17 -7.39 -9.99 -8.80 1.30 0.92 -7.77 -10.42 -10.82 -0.28
Mitochondria -1.84 -5.68 -1.53 -1.41 -1.25 -2.41 -5.54 1.82 2.55 6.36 -2.84 -7.26 -1.50 -0.76 -1.00
Mitotic Spindle -0.33 -0.01 -2.89 -2.84 -2.86 -1.84 -0.71 -4.83 -4.12 -4.18 -1.74 -0.51 -5.57 -5.01 -5.20
None -0.72 4.39 -2.24 -1.80 -4.78 -1.65 3.32 -5.47 -4.59 -7.56 -1.32 5.49 -5.25 -4.55 -8.82
Nuclear Periphery -1.76 -7.95 -4.09 -3.90 0.36 -5.10 -11.62 -11.38 -9.16 2.79 -5.34 -13.14 -9.06 -7.93 1.42
Nuclear Periphery Foci 3.29 1.31 0.32 -1.74 -0.56 1.99 -3.40 -0.91 -4.57 3.21 3.82 -1.33 0.45 -2.84 1.67
Nucleolus -1.31 0.68 0.49 1.50 -0.26 0.62 1.06 0.97 2.07 -1.47 -0.45 1.23 1.10 1.98 -0.68
Nucleus -11.14 -8.75 11.51 19.66 16.67 -5.54 6.57 28.23 39.45 11.39 -13.65 -2.10 25.67 40.21 20.70
Peroxisomes 1.06 1.03 0.87 -0.81 -0.80 -1.67 -0.38 0.13 1.77 0.74 -0.02 0.95 0.91 1.48 -0.14
Vacuole 1.85 -7.37 -11.93 -12.08 -7.50 -0.72 -8.40 -16.10 -16.08 -4.14 0.35 -10.57 -19.90 -19.96 -8.85
Vacuole Periphery -0.08 0.72 -1.39 -1.38 -1.42 0.49 0.89 -0.25 -0.51 -0.70 0.23 1.05 -1.41 -1.46 -1.60
Standard name
Human Ortholog
Description Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (75%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tda1

Tda1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tda1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available