Standard name | |
---|---|
Human Ortholog | |
Description | Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 5 | 5 | 2 | 3 | 5 | 4 | 11 | 6 | 6 | 5 | 10 | 12 | 4 | 3 | 5 | 2 | 1 | 2 | 8 | 3 | 2 | 7 | 9 |
Bud | 5 | 2 | 4 | 1 | 2 | 2 | 2 | 5 | 2 | 7 | 0 | 6 | 8 | 5 | 6 | 3 | 0 | 1 | 6 | 24 | 13 | 9 | 41 | 34 |
Bud Neck | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 31 | 41 | 12 | 17 | 17 | 18 |
Bud Site | 0 | 1 | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 2 | 9 | 10 | 19 | 20 | 16 | 20 | 27 | 3 | 8 | 11 | 6 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 18 | 35 | 18 | 26 | 86 | 141 | 166 | 195 | 124 | 143 | 135 | 147 | 109 | 244 | 391 | 123 | 50 | 63 | 7 | 20 | 8 | 11 | 30 | 15 |
Endoplasmic Reticulum | 0 | 3 | 1 | 0 | 6 | 5 | 9 | 20 | 12 | 10 | 13 | 12 | 7 | 8 | 7 | 10 | 3 | 5 | 2 | 5 | 1 | 0 | 3 | 0 |
Endosome | 0 | 2 | 8 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 6 | 6 | 0 | 5 | 18 | 8 | 3 | 19 | 10 |
Golgi | 0 | 12 | 1 | 4 | 2 | 1 | 2 | 5 | 0 | 0 | 0 | 0 | 32 | 33 | 29 | 75 | 46 | 38 | 1 | 1 | 2 | 4 | 3 | 2 |
Mitochondria | 15 | 17 | 8 | 2 | 0 | 6 | 12 | 17 | 14 | 23 | 13 | 24 | 13 | 0 | 0 | 29 | 6 | 17 | 1 | 2 | 1 | 2 | 2 | 1 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 105 | 131 | 58 | 6 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 48 | 11 | 5 | 81 | 51 | 67 | 19 | 66 | 8 | 24 | 19 | 9 |
Peroxisomes | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 2 | 2 | 0 | 2 | 5 | 7 | 5 | 6 | 18 | 11 |
SpindlePole | 4 | 2 | 29 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 5 | 1 | 2 | 41 | 15 | 8 | 79 | 82 | 80 | 89 | 202 | 160 |
Vac/Vac Membrane | 0 | 8 | 2 | 4 | 0 | 2 | 9 | 13 | 3 | 10 | 1 | 5 | 2 | 3 | 1 | 22 | 22 | 5 | 2 | 9 | 0 | 6 | 8 | 3 |
Unique Cell Count | 134 | 192 | 107 | 45 | 96 | 155 | 205 | 253 | 157 | 197 | 166 | 200 | 213 | 305 | 438 | 332 | 171 | 179 | 173 | 302 | 151 | 183 | 385 | 290 |
Labelled Cell Count | 151 | 222 | 145 | 52 | 102 | 173 | 219 | 288 | 182 | 219 | 188 | 231 | 245 | 321 | 457 | 403 | 204 | 210 | 173 | 302 | 151 | 183 | 385 | 290 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 38.5 | 43.8 | 23.9 | 23.3 | 21.9 | 18.8 | 15.6 | 16.2 | 16.1 | 14.2 | 15.1 | 14.8 | 55.6 | 57.3 | 59.4 | 40.3 | 46.2 | 51.4 | 40.6 | 39.3 | 38.4 |
Std Deviation (1e-4) | 11.6 | 15.8 | 9.1 | 8.8 | 6.3 | 5.1 | 4.4 | 4.6 | 3.8 | 3.8 | 3.5 | 3.4 | 16.7 | 16.0 | 16.1 | 12.9 | 16.6 | 20.6 | 14.9 | 14.5 | 14.0 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.13 | -0.35 | -0.61 | -0.56 | -0.57 | -0.75 | -0.67 | -0.69 | 1.22 | 1.26 | 1.31 | 0.75 | 0.95 | 1.1 | 0.76 | 0.72 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0.4 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 2.9 | 3.8 | 3.0 | 3.7 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5.1 | 10.4 | 12.1 | 10.9 | 10.6 | 9.9 | 9.3 | 10.5 | 9.5 | 5.9 | 11.7 | 15.5 | 3.9 | 2.3 | 3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.6 | 2.5 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 3.2 | 2.3 | 5.1 | 5.6 | 4.8 |
Mitochondria | 0 | 0 | -1.3 | -0.6 | -0.3 | 0.4 | 1.2 | 0.1 | 1.2 | -0.5 | 0 | 0 | 0.4 | -1.5 | 0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -4.7 | -8.5 | -10.4 | -11.3 | -12.6 | -10.4 | -11.1 | -10.7 | -11.6 | -5.7 | -12.1 | -15.4 | -5.8 | -4.1 | -2.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -3.5 | -5.5 | -6.9 | -7.6 | -8.4 | -6.9 | -7.7 | -7.1 | -7.7 | -6.8 | -9.2 | -10.7 | -3.6 | -4.1 | -5.5 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 3.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0203 | 0.0003 | 0.0285 | 0.005 | 0.0005 | 0.0107 | 0.0059 | 0.0027 | 0.0159 | 0.0078 | 0.0001 | 0.0068 | 0.0848 | 0.0011 | 0.0016 | 0.0006 | 0.0007 | 0.0008 |
Bud | 0.0033 | 0.0042 | 0.0242 | 0.0501 | 0.0007 | 0.0044 | 0.0025 | 0.0162 | 0.0075 | 0.0024 | 0.0002 | 0.0218 | 0.0009 | 0.0216 | 0.004 | 0.0035 | 0.0004 | 0.0027 |
Bud Neck | 0.0006 | 0.0099 | 0.0007 | 0.0007 | 0.0003 | 0.0254 | 0.006 | 0.0055 | 0.0049 | 0.0013 | 0.0014 | 0.0067 | 0.0006 | 0.011 | 0.001 | 0.001 | 0.0003 | 0.0011 |
Bud Periphery | 0.0022 | 0.0005 | 0.0069 | 0.0191 | 0.0002 | 0.0012 | 0.0009 | 0.0046 | 0.0021 | 0.0022 | 0 | 0.0065 | 0.0008 | 0.0078 | 0.0028 | 0.0008 | 0.0001 | 0.0014 |
Bud Site | 0.0022 | 0.0309 | 0.0125 | 0.0079 | 0.0002 | 0.0042 | 0.019 | 0.0452 | 0.038 | 0.0011 | 0.0003 | 0.0034 | 0.0081 | 0.0532 | 0.0158 | 0.003 | 0.0008 | 0.0031 |
Cell Periphery | 0.0009 | 0.0017 | 0.0014 | 0.0004 | 0.0002 | 0.0012 | 0.0007 | 0.0036 | 0.0009 | 0.0023 | 0.0001 | 0.0016 | 0.0005 | 0.0055 | 0.005 | 0.0002 | 0.0001 | 0.0008 |
Cytoplasm | 0.0602 | 0.1294 | 0.0592 | 0.0779 | 0.1966 | 0.0448 | 0.0364 | 0.1667 | 0.0808 | 0.0054 | 0.1239 | 0.1606 | 0.0005 | 0.0003 | 0.0007 | 0.0013 | 0.0033 | 0.0415 |
Cytoplasmic Foci | 0.6424 | 0.5957 | 0.6873 | 0.6534 | 0.5617 | 0.672 | 0.7666 | 0.5914 | 0.6687 | 0.5989 | 0.7626 | 0.6603 | 0.1824 | 0.4288 | 0.6446 | 0.6047 | 0.8701 | 0.6078 |
Eisosomes | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0003 | 0.0001 | 0.0012 | 0.0004 | 0.0001 | 0.0001 | 0.0019 | 0.0002 | 0.0001 | 0.0026 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0042 |
Endosome | 0.0021 | 0.0018 | 0.0069 | 0.0026 | 0.019 | 0.0036 | 0.0203 | 0.0014 | 0.0036 | 0.0102 | 0.0016 | 0.0147 | 0.0113 | 0.0002 | 0.0006 | 0.0002 | 0.0056 | 0.0048 |
Golgi | 0.0009 | 0.0007 | 0.0029 | 0.0014 | 0.0052 | 0.0025 | 0.006 | 0.0011 | 0.0021 | 0.0026 | 0.002 | 0.0041 | 0.0254 | 0.0006 | 0.0008 | 0.0007 | 0.0056 | 0.0016 |
Lipid Particles | 0.0541 | 0.0153 | 0.0161 | 0.0129 | 0.0233 | 0.0197 | 0.0375 | 0.0184 | 0.0255 | 0.0336 | 0.015 | 0.0108 | 0.163 | 0.0619 | 0.0202 | 0.0558 | 0.0798 | 0.0306 |
Mitochondria | 0.0109 | 0.0171 | 0.0164 | 0.0089 | 0.0452 | 0.0282 | 0.0187 | 0.017 | 0.0293 | 0.0862 | 0.0172 | 0.0094 | 0.1379 | 0.1014 | 0.0503 | 0.2571 | 0.0028 | 0.0126 |
None | 0.0005 | 0.0006 | 0.0013 | 0.007 | 0.0006 | 0.0032 | 0.0044 | 0.0007 | 0.0044 | 0.0012 | 0.0002 | 0.0012 | 0.001 | 0.0006 | 0.0008 | 0.0013 | 0.0005 | 0.0013 |
Nuclear Periphery | 0.0033 | 0.0004 | 0.0019 | 0.0038 | 0.0003 | 0.0002 | 0.0026 | 0.0003 | 0.0004 | 0.0262 | 0.0001 | 0.0005 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0417 |
Nucleolus | 0.1544 | 0.1434 | 0.0845 | 0.093 | 0.1197 | 0.1129 | 0.0209 | 0.0727 | 0.066 | 0.1267 | 0.0229 | 0.0362 | 0.2475 | 0.2257 | 0.2003 | 0.0177 | 0.0047 | 0.1788 |
Nucleus | 0.0063 | 0.007 | 0.0052 | 0.0101 | 0.0031 | 0.0088 | 0.0016 | 0.0081 | 0.0066 | 0.0151 | 0.0008 | 0.0059 | 0.0061 | 0.0176 | 0.0136 | 0.0009 | 0.0009 | 0.0271 |
Peroxisomes | 0.0144 | 0.0159 | 0.0133 | 0.0087 | 0.0056 | 0.021 | 0.0315 | 0.0294 | 0.0302 | 0.05 | 0.0463 | 0.016 | 0.1135 | 0.0433 | 0.0209 | 0.0477 | 0.0088 | 0.0123 |
Punctate Nuclear | 0.0033 | 0.0118 | 0.0147 | 0.032 | 0.0018 | 0.0106 | 0.01 | 0.0049 | 0.0082 | 0.0047 | 0.0039 | 0.01 | 0.0121 | 0.0127 | 0.0036 | 0.0016 | 0.0134 | 0.0205 |
Vacuole | 0.0106 | 0.0103 | 0.0085 | 0.0028 | 0.0062 | 0.0119 | 0.0027 | 0.008 | 0.0034 | 0.0081 | 0.0008 | 0.0168 | 0.0009 | 0.005 | 0.0092 | 0.0005 | 0.001 | 0.0025 |
Vacuole Periphery | 0.0067 | 0.0031 | 0.0058 | 0.0018 | 0.0093 | 0.0133 | 0.0034 | 0.0017 | 0.0011 | 0.0112 | 0.0004 | 0.0064 | 0.0012 | 0.0014 | 0.0032 | 0.0011 | 0.0009 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.0152 | 41.8229 | 51.0873 | 57.2607 | 35.9286 | 69.7789 | 56.7622 | 54.6668 | 58.6757 | 61.5325 |
Translational Efficiency | 1.1996 | 1.6129 | 1.1985 | 1.1308 | 1.651 | 1.1712 | 1.4339 | 1.1291 | 1.193 | 1.3125 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2283 | 669 | 255 | 1944 | 1566 | 1679 | 535 | 140 | 3849 | 2348 | 790 | 2084 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2471.55 | 3229.53 | 2828.72 | 2683.62 | 2924.02 | 3086.16 | 2653.31 | 2744.42 | 2655.64 | 3127.01 | 2709.93 | 2687.70 |
Standard Deviation | 624.47 | 889.56 | 443.81 | 527.81 | 802.99 | 781.87 | 408.01 | 482.44 | 736.92 | 816.57 | 427.83 | 525.10 |
Intensity Change Log 2 | — | 0.385908 | 0.194733 | 0.118764 | — | 0.077860 | -0.140160 | -0.091452 | — | 0.227165 | 0.022935 | 0.008656 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000281 | 0.000599 | 0.001892 | 0.000178 | 0.001271 | 0.000723 | 0.000197 | 0.000267 | 0.000684 | 0.000688 | 0.000744 | 0.000184 |
Bud Neck | 0.015945 | 0.008937 | 0.008591 | 0.001487 | 0.010976 | 0.012956 | 0.002250 | 0.003754 | 0.013923 | 0.011811 | 0.004297 | 0.001640 |
Bud Site | 0.128564* | 0.087260 | 0.007741 | 0.007736 | 0.088612 | 0.101276* | 0.003695 | 0.011195 | 0.112309* | 0.097282 | 0.005001 | 0.007968 |
Cell Periphery | 0.000495 | 0.000735 | 0.002305 | 0.000356 | 0.000429 | 0.000417 | 0.000981 | 0.001240 | 0.000468 | 0.000508 | 0.001409 | 0.000415 |
Cytoplasm | 0.105829* | 0.068069 | 0.656130* | 0.854943* | 0.066066 | 0.078985 | 0.861746* | 0.804681* | 0.089651 | 0.075874 | 0.795376* | 0.851566* |
Cytoplasmic Foci | 0.661871* | 0.755411* | 0.027917 | 0.005311 | 0.733494* | 0.717229* | 0.010705 | 0.015512 | 0.691012* | 0.728108* | 0.016261 | 0.005996 |
Eisosomes | 0.000060 | 0.000112 | 0.000007 | 0.000002 | 0.000050 | 0.000048 | 0.000002 | 0.000003 | 0.000056 | 0.000067 | 0.000004 | 0.000002 |
Endoplasmic Reticulum | 0.000230 | 0.000111 | 0.000429 | 0.000095 | 0.000061 | 0.000166 | 0.000098 | 0.000054 | 0.000161 | 0.000150 | 0.000205 | 0.000092 |
Endosome | 0.007556 | 0.004349 | 0.005300 | 0.002012 | 0.007669 | 0.004695 | 0.000935 | 0.001000 | 0.007602 | 0.004596 | 0.002344 | 0.001944 |
Golgi | 0.008755 | 0.007879 | 0.011868 | 0.003228 | 0.010158 | 0.006224 | 0.004087 | 0.003116 | 0.009326 | 0.006695 | 0.006599 | 0.003220 |
Lipid Particles | 0.001221 | 0.004467 | 0.016179 | 0.002282 | 0.002569 | 0.001004 | 0.005729 | 0.011979 | 0.001769 | 0.001991 | 0.009102 | 0.002934 |
Mitochondria | 0.000625 | 0.000360 | 0.000116 | 0.000152 | 0.000721 | 0.000682 | 0.000045 | 0.000019 | 0.000664 | 0.000590 | 0.000068 | 0.000143 |
Mitotic Spindle | 0.012957 | 0.007993 | 0.008876 | 0.005126 | 0.013998 | 0.018349 | 0.002396 | 0.000666 | 0.013381 | 0.015399 | 0.004487 | 0.004826 |
None | 0.000879 | 0.000541 | 0.001646 | 0.000967 | 0.000443 | 0.000836 | 0.001956 | 0.000991 | 0.000702 | 0.000752 | 0.001856 | 0.000969 |
Nuclear Periphery | 0.000083 | 0.000066 | 0.000918 | 0.000103 | 0.000090 | 0.000168 | 0.000204 | 0.000119 | 0.000086 | 0.000139 | 0.000434 | 0.000104 |
Nuclear Periphery Foci | 0.002531 | 0.002452 | 0.013537 | 0.001482 | 0.002719 | 0.002382 | 0.005291 | 0.004894 | 0.002608 | 0.002402 | 0.007953 | 0.001711 |
Nucleolus | 0.001693 | 0.001609 | 0.000199 | 0.000287 | 0.001362 | 0.002616 | 0.000093 | 0.000181 | 0.001559 | 0.002329 | 0.000127 | 0.000280 |
Nucleus | 0.002416 | 0.002861 | 0.045859 | 0.022937 | 0.001982 | 0.004313 | 0.048779 | 0.023099 | 0.002239 | 0.003899 | 0.047836 | 0.022948 |
Peroxisomes | 0.030320 | 0.029375 | 0.000083 | 0.000221 | 0.037436 | 0.026568 | 0.000030 | 0.000156 | 0.033215 | 0.027368 | 0.000047 | 0.000217 |
Vacuole | 0.014329 | 0.014843 | 0.184299 | 0.086702 | 0.016667 | 0.015798 | 0.049994 | 0.115667 | 0.015280 | 0.015526 | 0.093345 | 0.088648 |
Vacuole Periphery | 0.003361 | 0.001971 | 0.006109 | 0.004393 | 0.003227 | 0.004566 | 0.000787 | 0.001407 | 0.003307 | 0.003827 | 0.002505 | 0.004193 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.11 | -1.33 | 2.69 | 2.78 | 1.48 | 0.67 | 1.46 | 1.31 | 1.24 | -1.24 | -0.26 | -0.06 | 1.96 | 2.74 | 1.79 |
Bud Neck | 5.78 | 1.01 | 16.13 | 8.49 | 9.94 | -2.33 | 9.90 | 3.24 | 4.82 | -3.20 | 1.99 | 8.85 | 19.75 | 15.38 | 9.16 |
Bud Site | 5.52 | 27.75* | 29.41* | 13.64 | 2.24 | -2.29 | 23.11* | 17.96* | 19.11* | -3.86 | 3.30 | 36.49 | 35.94 | 26.40* | -0.82 |
Cell Periphery | -1.60 | -5.26 | 3.96 | 2.98 | 5.90 | 0.03 | -3.40 | -3.94 | -4.10 | -0.31 | -0.88 | -5.97 | 2.92 | 3.26 | 6.83 |
Cytoplasm | 4.59 | -22.50 | -105.45* | -88.22* | -12.25 | -2.36 | -68.99* | -26.64 | -25.92 | 4.64 | 2.62 | -60.35 | -121.68* | -117.04* | -8.03 |
Cytoplasmic Foci | -6.57 | 81.86* | 93.59* | 64.99* | 10.08 | 2.12 | 98.16* | 88.66* | 80.69* | -2.80 | -3.68 | 126.47 | 132.96 | 110.42* | 10.10 |
Eisosomes | -2.51 | 9.55 | 11.58 | 5.63 | 4.17 | -0.02 | 6.57 | 6.26 | 11.56 | -2.75 | -1.46 | 12.13 | 13.04 | 10.50 | 5.00 |
Endoplasmic Reticulum | 1.26 | -1.28 | 1.69 | 0.94 | 3.85 | -2.17 | -3.81 | -0.38 | 2.09 | 3.38 | 0.26 | -0.64 | 1.59 | 2.06 | 4.39 |
Endosome | 3.01 | 1.40 | 8.25 | 3.40 | 6.91 | 3.13 | 7.90 | 7.31 | 6.72 | -1.32 | 4.48 | 8.25 | 10.59 | 7.06 | 4.00 |
Golgi | 0.38 | -1.33 | 7.22 | 3.69 | 7.67 | 3.24 | 5.60 | 7.60 | 5.03 | 1.48 | 2.70 | 4.12 | 9.88 | 6.20 | 5.22 |
Lipid Particles | -2.36 | -7.85 | -5.88 | 1.05 | 6.95 | 3.84 | -5.44 | -4.53 | -5.15 | -2.61 | -0.74 | -9.91 | -6.05 | -3.47 | 7.43 |
Mitochondria | 1.86 | 3.73 | 3.87 | 4.07 | 0.63 | -0.03 | 2.59 | 2.75 | 7.48 | 6.00 | 0.29 | 4.49 | 4.25 | 7.16 | -1.74 |
Mitotic Spindle | 1.53 | 0.38 | 5.15 | 2.07 | 2.99 | -2.38 | 6.28 | 8.06 | 9.62 | 3.21 | -2.03 | 5.57 | 6.72 | 7.19 | 1.37 |
None | 1.36 | -1.88 | -0.06 | -2.25 | 2.75 | -2.06 | -6.39 | -1.56 | 0.66 | 4.50 | -0.26 | -4.81 | -1.11 | -0.83 | 4.56 |
Nuclear Periphery | 1.88 | -9.68 | -4.46 | -5.93 | 9.13 | -4.40 | -8.96 | -5.09 | -0.89 | 3.13 | -4.23 | -12.18 | -5.21 | 0.58 | 10.52 |
Nuclear Periphery Foci | 0.20 | -8.72 | 2.28 | 1.36 | 9.48 | 0.22 | -5.28 | -3.85 | -3.95 | 0.10 | 0.09 | -10.10 | 2.05 | 1.53 | 11.56 |
Nucleolus | 0.66 | 5.05 | 4.89 | 3.01 | -2.17 | -1.56 | 3.85 | 3.67 | 4.33 | -2.25 | -1.17 | 6.48 | 5.98 | 4.85 | -7.16 |
Nucleus | -0.28 | -11.39 | -23.88 | -22.48 | 4.60 | -3.81 | -17.80 | -7.65 | -6.90 | 6.07 | -3.67 | -21.12 | -25.17 | -22.51 | 8.90 |
Peroxisomes | 0.40 | 17.00 | 16.97 | 10.08 | -1.04 | 3.55 | 14.25 | 14.15 | 16.17 | -2.46 | 2.98 | 22.11 | 22.03 | 19.09 | -3.17 |
Vacuole | -0.76 | -13.48 | -25.51 | -22.86 | 6.89 | -0.19 | -11.44 | -10.47 | -10.45 | -5.86 | -1.04 | -16.98 | -27.47 | -26.50 | 1.88 |
Vacuole Periphery | 1.96 | -1.00 | 0.80 | -1.13 | 1.52 | -1.76 | 4.77 | 3.90 | 5.47 | -0.67 | -1.06 | 3.14 | 1.11 | 1.89 | -1.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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