Standard name
Human Ortholog
Description Cyclin-like component of the RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; forms a kinase-cyclin pair in the RNAPII holoenzyme with Ssn3p; required for both entry into and execution of the meiotic program; involved in glucose repression and telomere maintenance; cyclin homolog 35% identical to human cyclin C
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.1 0.08 0.15 0.08 0.08 0.09 0.07 0.09 0.05 0.06 0.13 0.17 0.13 0.11 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0 0 0.13 0.13 0.35 0.56 0.41 0.71 0.51 0.7 0.43 0 0 0 0 0 0.06 0 0 0 0 0.05 0
Nucleus 0.82 0.9 0.85 0.82 0.75 0.75 0.69 0.73 0.62 0.7 0.66 0.63 0.83 0.81 0.83 0.82 0.8 0.77 0.77 0.73 0.67 0.65 0.54 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.09 0.08 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.05 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05
Vac/Vac Membrane 0.06 0.07 0 0 0 0 0 0 0 0 0 0.12 0.05 0 0 0.06 0.06 0.08 0.1 0.14 0.13 0.11 0.15 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 2 1 3 1 4 1 2 1 0 0 0 0 0 1 3 1 1 1 5 7
Bud Neck 0 0 3 0 3 2 1 2 1 0 0 1 0 0 0 0 0 0 0 0 1 2 0 2
Bud Site 2 0 0 0 1 4 10 7 5 5 9 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 3 19 16 43 18 23 22 22 30 14 19 14 35 34 46 31 42 4 0 4 2 3 4
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 15 10 15 0 0 0 0 0 1
Endosome 2 0 0 2 3 4 0 2 0 0 0 7 1 1 2 5 5 6 2 2 1 1 6 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 2 2 0 0 1 1 4 0
Mitochondria 69 1 3 25 36 78 154 105 220 176 197 129 0 0 0 12 8 23 3 1 3 2 10 7
Nucleus 163 81 162 155 211 170 191 187 194 244 184 186 90 170 211 347 247 323 168 80 143 104 123 129
Nuclear Periphery 0 0 1 0 1 1 1 0 1 0 0 1 0 0 0 1 0 1 1 0 0 1 1 1
Nucleolus 13 0 1 0 0 2 1 0 1 2 2 2 0 0 0 0 2 2 4 5 17 15 18 15
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 13 10 7 2 4 16 2 10 1 5 2 6 5 7 9 3 7 2 6 8 12 11
Vac/Vac Membrane 11 6 1 0 7 3 4 1 9 8 8 37 5 8 8 25 20 35 21 14 27 17 33 25
Unique Cell Count 200 90 190 189 282 226 276 257 312 347 280 297 109 209 254 421 309 417 219 110 215 161 228 222
Labelled Cell Count 269 92 206 210 314 285 393 343 461 476 417 394 113 220 260 460 334 453 219 110 215 161 228 222


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.5 4.9 5.2 5.0 4.0 3.8 4.5 3.7 4.0 3.6 4.2 5.8 6.0 5.9 6.6 6.9 6.5 5.5 6.0 6.1
Std Deviation (1e-4) 0.7 1.1 0.8 1.7 1.9 1.1 1.2 1.6 0.8 1.1 0.8 1.0 1.3 1.4 1.4 1.5 1.7 1.6 1.3 1.8 1.7
Intensity Change (Log2) – – – 0.07 0.02 -0.29 -0.39 -0.12 -0.43 -0.3 -0.46 -0.22 0.25 0.3 0.26 0.43 0.49 0.4 0.15 0.29 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 1.7 -0.7 -0.6 -0.5 -1.2 -0.5 -2.1 -1.4 0.8 2.0 1.1 0.3 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 4.3 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Nucleus -0.9 -2.7 -2.5 -4.0 -3.2 -5.5 -3.9 -4.7 -5.4 -0.6 -1.0 -0.6 -0.9 -1.5 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.6 -2.3 0 0 0 0 0 0 0 -1.9 -1.9 -2.6 -3.3 -2.1 -4.4
Vacuole 0 0 0 0 0 0 0 0 4.8 0 0 0 3.1 3.2 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3919 0.5642 0.398 0.0122 0.0435 0.4186 0.5897 0.7943 0.7015 0.5021 0.1138 0.6051 0.5885 0.0259 0.7709 0.5164 0.6274 0.2324
Actin 0.0098 0.0001 0.0011 0.0002 0.0052 0.0099 0.01 0 0.0028 0 0.0001 0.0005 0.0617 0.0001 0.0161 0.0153 0.003 0.0122
Bud 0.0003 0.0018 0 0.0027 0.0001 0.0001 0.0032 0 0.0048 0 0 0 0.0006 0.0002 0.0005 0.0003 0.0005 0.0001
Bud Neck 0.0017 0.0006 0.0003 0.0005 0.0012 0.0039 0.0056 0.0003 0.0004 0.0001 0.0007 0.001 0.0063 0.0004 0.0015 0.0008 0.0019 0.0016
Bud Periphery 0.0005 0.0002 0 0.0004 0.0003 0.0001 0.0018 0 0.0013 0 0 0.0001 0.0011 0.0001 0.0007 0.0007 0.0014 0.0001
Bud Site 0.0006 0.001 0.0001 0.0001 0.0003 0.0001 0.0027 0.0001 0.0008 0 0 0.0001 0.0095 0.0005 0.0159 0.0005 0.0038 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0004 0 0 0 0 0 0.0007 0 0.0007 0.0001 0.0006 0
Cytoplasm 0.0207 0.02 0.0046 0.0097 0.0037 0.0009 0.0086 0.0023 0.002 0.0009 0.0036 0.0005 0.0058 0.0057 0.0113 0.0115 0.0022 0.0028
Cytoplasmic Foci 0.0046 0.0016 0.0002 0.0003 0.0031 0.0005 0.0104 0.0001 0.0013 0 0.0001 0.0004 0.041 0.0024 0.0087 0.0008 0.0009 0.001
Eisosomes 0.0002 0 0 0 0.0001 0 0.0003 0 0 0 0 0 0.002 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0044 0.0007 0.0036 0.0004 0.0019 0.0011 0.0046 0.0002 0.0003 0 0.0001 0.0001 0.0056 0.0004 0.0182 0.0013 0.0014 0.0033
Endosome 0.0238 0.0032 0.0028 0.0003 0.0246 0.0044 0.0144 0.0002 0.0038 0 0.0001 0.0012 0.0246 0.0004 0.0032 0.0085 0.007 0.0062
Golgi 0.0026 0 0.0002 0 0.0031 0.0013 0.0033 0 0.0009 0 0 0.0005 0.0115 0 0.0017 0.006 0.0022 0.0023
Lipid Particles 0.006 0.0001 0.0003 0 0.0157 0.0004 0.0186 0 0.0018 0 0 0.0015 0.0218 0.0001 0.0038 0.0014 0.0058 0.0019
Mitochondria 0.0022 0.0007 0.0003 0.0003 0.0122 0.0004 0.0163 0.0002 0.0012 0.0001 0.0001 0.0019 0.0134 0.0002 0.0014 0.004 0.0045 0.0005
None 0.0344 0.0168 0.0024 0.0185 0.0029 0.0134 0.028 0.0091 0.0073 0.0001 0.0427 0.0065 0.0055 0.002 0.0016 0.0059 0.001 0.0014
Nuclear Periphery 0.0353 0.0127 0.0136 0.0224 0.0279 0.0068 0.0326 0.0089 0.0118 0.0008 0.0062 0.0044 0.0211 0.0075 0.01 0.0067 0.029 0.0137
Nucleolus 0.0101 0.0108 0.0063 0.0069 0.013 0.018 0.0215 0.009 0.0085 0.0048 0.0323 0.0208 0.0079 0.0082 0.0062 0.009 0.022 0.0173
Nucleus 0.8018 0.8996 0.9487 0.9183 0.8429 0.9156 0.7837 0.9476 0.934 0.9899 0.8947 0.9435 0.7137 0.9432 0.86 0.9135 0.8936 0.9152
Peroxisomes 0.0013 0 0 0 0.0058 0.0001 0.0069 0 0.0072 0 0 0.0022 0.0175 0 0.0047 0.0003 0.0005 0.0005
Punctate Nuclear 0.0357 0.0279 0.0148 0.0183 0.0327 0.0224 0.0227 0.0217 0.009 0.0033 0.0189 0.0145 0.0249 0.0283 0.0322 0.0116 0.0106 0.0192
Vacuole 0.0022 0.0016 0.0005 0.0003 0.0013 0.0003 0.0028 0.0002 0.0004 0 0.0001 0.0002 0.0022 0.0002 0.0011 0.0007 0.0049 0.0003
Vacuole Periphery 0.0017 0.0003 0.0003 0.0003 0.0019 0.0002 0.0018 0.0001 0.0003 0 0.0001 0.0002 0.0016 0.0001 0.0004 0.0008 0.0031 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.1899 13.1923 6.0241 14.1252 10.3028 2.2691 11.7996 11.0803 10.1217 13.1568
Translational Efficiency 0.5295 0.5545 1.031 0.4503 0.4669 4.1802 0.6354 0.4584 0.5392 0.5199

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cyclin-like component of the RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; forms a kinase-cyclin pair in the RNAPII holoenzyme with Ssn3p; required for both entry into and execution of the meiotic program; involved in glucose repression and telomere maintenance; cyclin homolog 35% identical to human cyclin C
Localization
Cell Percentages nucleus (87%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ssn8

Ssn8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssn8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available