Standard name | |
---|---|
Human Ortholog | |
Description | Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Bud | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 3 | 0 | 3 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 3 | 1 | 2 | 1 | 0 | 0 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 274 | 122 | 109 | 100 | 87 | 155 | 197 | 178 | 101 | 171 | 83 | 100 | 225 | 223 | 269 | 129 | 113 | 46 | 277 | 115 | 109 | 115 | 169 | 127 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 10 | 7 | 2 | 0 | 0 | 1 | 0 | 3 | 5 |
Endosome | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 3 | 0 | 2 | 1 | 2 | 0 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 16 | 1 | 3 | 13 | 4 | 38 | 59 | 66 | 73 | 113 | 83 | 81 | 0 | 3 | 0 | 5 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 0 |
Nucleus | 2 | 0 | 0 | 3 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 1 | 1 | 0 | 0 | 7 | 3 | 1 | 6 | 1 | 3 | 0 | 0 | 0 | 2 | 6 | 2 | 1 | 6 | 3 | 0 | 0 | 0 |
Unique Cell Count | 287 | 124 | 115 | 108 | 89 | 164 | 228 | 208 | 147 | 223 | 145 | 151 | 228 | 224 | 271 | 135 | 120 | 52 | 299 | 127 | 131 | 121 | 188 | 138 |
Labelled Cell Count | 300 | 127 | 118 | 125 | 95 | 197 | 269 | 251 | 176 | 297 | 174 | 189 | 231 | 227 | 272 | 154 | 132 | 58 | 299 | 127 | 131 | 121 | 188 | 138 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.8 | 5.4 | 4.9 | 5.3 | 4.6 | 4.2 | 4.1 | 4.5 | 3.9 | 4.1 | 3.8 | 5.8 | 5.4 | 5.6 | 7.1 | 9.1 | 8.8 | 6.8 | 7.4 | 8.7 |
Std Deviation (1e-4) | 0.6 | 1.2 | 1.4 | 1.3 | 1.7 | 1.4 | 1.6 | 1.0 | 1.9 | 1.5 | 1.4 | 1.5 | 0.9 | 1.1 | 1.1 | 1.9 | 3.3 | 2.5 | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.03 | -0.23 | -0.37 | -0.39 | -0.27 | -0.48 | -0.41 | -0.51 | 0.09 | 0.01 | 0.05 | 0.4 | 0.75 | 0.71 | 0.33 | 0.44 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 1.1 | -0.1 | -2.4 | -2.5 | -5.3 | -4.2 | -6.8 | -5.6 | 2.1 | 2.9 | 2.8 | 0.3 | -0.2 | -1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0007 | 1.5515 | 1.1727 | 1.0887 | 1.0047 | 1.4475 | 2.1143 | 2.738 | 2.3619 | 2.1799 | 2.1918 | 2.0448 | 3.2482 | 3.0783 | 2.8384 | 2.9202 | 2.4655 | 2.9749 |
Actin | 0.0171 | 0.0003 | 0.0074 | 0.0009 | 0.0402 | 0.0047 | 0.0457 | 0.0234 | 0.0227 | 0.0669 | 0.035 | 0.0007 | 0.1114 | 0.0002 | 0.0442 | 0.0006 | 0.0002 | 0.0176 |
Bud | 0.0002 | 0.003 | 0.0021 | 0.0001 | 0.0001 | 0.0007 | 0.001 | 0.0021 | 0.0034 | 0.0003 | 0.003 | 0.0004 | 0.0011 | 0 | 0.0008 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0031 | 0.0002 | 0.0008 | 0.0002 | 0.0012 | 0.0027 | 0.0259 | 0.0002 | 0.0023 | 0.0002 | 0.0016 | 0.0009 | 0.0064 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0004 |
Bud Periphery | 0.0004 | 0.0009 | 0.0034 | 0.0001 | 0.0001 | 0.0009 | 0.0019 | 0.0015 | 0.0064 | 0.0003 | 0.0049 | 0.0005 | 0.0018 | 0 | 0.0011 | 0 | 0 | 0.0001 |
Bud Site | 0.0011 | 0.0033 | 0.0082 | 0.0002 | 0.0003 | 0.0018 | 0.0099 | 0.0132 | 0.0112 | 0.0009 | 0.0117 | 0.0001 | 0.0053 | 0.0003 | 0.0024 | 0.0001 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0014 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 |
Cytoplasm | 0.5129 | 0.6422 | 0.6047 | 0.6515 | 0.6055 | 0.6778 | 0.3255 | 0.4779 | 0.4926 | 0.5156 | 0.599 | 0.6729 | 0.4054 | 0.7433 | 0.5323 | 0.6878 | 0.675 | 0.5949 |
Cytoplasmic Foci | 0.0227 | 0.0072 | 0.0126 | 0.0109 | 0.0061 | 0.0124 | 0.0441 | 0.0083 | 0.0189 | 0.0052 | 0.0523 | 0.0084 | 0.0286 | 0.0031 | 0.0139 | 0.0131 | 0.0018 | 0.0071 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0008 | 0 | 0.0009 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0041 | 0.0043 | 0.0033 | 0.0057 | 0.0027 | 0.0016 | 0.0081 | 0.0021 | 0.0033 | 0.0016 | 0.0016 | 0.0027 | 0.0061 | 0.0018 | 0.0029 | 0.0019 | 0.0008 | 0.0023 |
Endosome | 0.0087 | 0.0039 | 0.0053 | 0.0067 | 0.0017 | 0.0043 | 0.0471 | 0.0026 | 0.0131 | 0.0016 | 0.0173 | 0.0039 | 0.0196 | 0.0004 | 0.0071 | 0.0144 | 0.0003 | 0.0018 |
Golgi | 0.0041 | 0.0003 | 0.0017 | 0.0004 | 0.0016 | 0.0016 | 0.0245 | 0.003 | 0.0065 | 0.002 | 0.0162 | 0.0001 | 0.0124 | 0.0001 | 0.0047 | 0.0006 | 0 | 0.0008 |
Lipid Particles | 0.0038 | 0.0005 | 0.0062 | 0.0005 | 0.0026 | 0.0039 | 0.0186 | 0.0016 | 0.0072 | 0.0003 | 0.02 | 0.0001 | 0.0133 | 0.0001 | 0.0081 | 0.0004 | 0 | 0.0002 |
Mitochondria | 0.0056 | 0.0002 | 0.0079 | 0.0002 | 0.0012 | 0.001 | 0.0084 | 0.0027 | 0.0024 | 0.0005 | 0.0081 | 0.0001 | 0.0046 | 0.0001 | 0.0088 | 0.0002 | 0.0001 | 0.0002 |
None | 0.3925 | 0.3268 | 0.3182 | 0.317 | 0.318 | 0.2719 | 0.3993 | 0.4489 | 0.385 | 0.4018 | 0.1966 | 0.3061 | 0.3447 | 0.2481 | 0.3365 | 0.2419 | 0.3206 | 0.3712 |
Nuclear Periphery | 0.0016 | 0.0008 | 0.001 | 0.0013 | 0.0011 | 0.0017 | 0.004 | 0.0003 | 0.0024 | 0.0004 | 0.0008 | 0.0003 | 0.0036 | 0.0003 | 0.0073 | 0.0006 | 0.0001 | 0.0007 |
Nucleolus | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0018 | 0.0022 | 0.0001 | 0.0018 | 0 | 0.0003 | 0.0001 | 0.0008 | 0 | 0.0027 | 0 | 0 | 0 |
Nucleus | 0.0029 | 0.0021 | 0.0019 | 0.0017 | 0.0024 | 0.0031 | 0.0028 | 0.0007 | 0.0049 | 0.0005 | 0.001 | 0.0009 | 0.0023 | 0.0011 | 0.0069 | 0.0011 | 0.0005 | 0.0005 |
Peroxisomes | 0.0147 | 0.0001 | 0.0114 | 0.0002 | 0.0119 | 0.003 | 0.0197 | 0.01 | 0.0049 | 0.0007 | 0.0257 | 0.0001 | 0.0115 | 0 | 0.0089 | 0.0339 | 0 | 0.0009 |
Punctate Nuclear | 0.0017 | 0.0006 | 0.0012 | 0.0009 | 0.002 | 0.0036 | 0.0034 | 0.0004 | 0.0039 | 0.0002 | 0.003 | 0.0009 | 0.0174 | 0.0003 | 0.0069 | 0.0005 | 0.0001 | 0.0005 |
Vacuole | 0.0015 | 0.0025 | 0.0017 | 0.0009 | 0.0004 | 0.0011 | 0.0048 | 0.0005 | 0.0056 | 0.0007 | 0.0011 | 0.0005 | 0.0016 | 0.0005 | 0.0014 | 0.0019 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0 | 0.0008 | 0.0006 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.758 | 17.4461 | 12.4479 | 13.3524 | 19.4641 | 9.0024 | 15.9436 | 11.9056 | 13.3854 | 12.7701 |
Translational Efficiency | 0.6636 | 0.7475 | 0.814 | 0.6741 | 0.4981 | 0.7981 | 0.7986 | 1.0311 | 0.67 | 0.8071 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1885 | 1115 | 2266 | 1149 | 1075 | 1703 | 160 | 257 | 2960 | 2818 | 2426 | 1406 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 699.09 | 752.99 | 935.09 | 913.06 | 771.31 | 824.85 | 1051.52 | 963.16 | 725.32 | 796.42 | 942.77 | 922.22 |
Standard Deviation | 95.69 | 107.01 | 112.12 | 124.43 | 93.95 | 107.04 | 105.29 | 133.82 | 101.21 | 112.65 | 115.36 | 127.67 |
Intensity Change Log 2 | — | 0.107153 | 0.419627 | 0.385231 | — | 0.096821 | 0.447094 | 0.320465 | — | 0.101742 | 0.434100 | 0.351620 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000066 | 0.000278 | 0.000246 | 0.003498 | 0.000069 | 0.000311 | 0.000506 | 0.001269 | 0.000067 | 0.000298 | 0.000263 | 0.003091 |
Bud Neck | 0.013911 | 0.013603 | 0.004932 | 0.010768 | 0.007336 | 0.020881 | 0.001761 | 0.003962 | 0.011523 | 0.018001 | 0.004723 | 0.009524 |
Bud Site | 0.010633 | 0.013250 | 0.006463 | 0.098204 | 0.002867 | 0.022995 | 0.004286 | 0.082861 | 0.007813 | 0.019139 | 0.006319 | 0.095399 |
Cell Periphery | 0.000140 | 0.000087 | 0.000051 | 0.000268 | 0.000045 | 0.000111 | 0.000226 | 0.000202 | 0.000105 | 0.000101 | 0.000062 | 0.000256 |
Cytoplasm | 0.646587* | 0.592595* | 0.590649* | 0.437786* | 0.675618* | 0.541789* | 0.431377* | 0.396792* | 0.657130* | 0.561892* | 0.580144* | 0.430293* |
Cytoplasmic Foci | 0.098583 | 0.129776 | 0.006165 | 0.029854 | 0.080892 | 0.131250 | 0.014328 | 0.058101 | 0.092158 | 0.130667 | 0.006704 | 0.035017 |
Eisosomes | 0.000077 | 0.000049 | 0.000011 | 0.000045 | 0.000039 | 0.000075 | 0.000100 | 0.000040 | 0.000063 | 0.000065 | 0.000017 | 0.000044 |
Endoplasmic Reticulum | 0.000603 | 0.000667 | 0.001015 | 0.002125 | 0.000264 | 0.000391 | 0.005910 | 0.001988 | 0.000480 | 0.000500 | 0.001338 | 0.002100 |
Endosome | 0.003265 | 0.016315 | 0.000861 | 0.028089 | 0.002932 | 0.018296 | 0.006132 | 0.026936 | 0.003144 | 0.017512 | 0.001209 | 0.027878 |
Golgi | 0.000781 | 0.003071 | 0.000105 | 0.021843 | 0.000264 | 0.003189 | 0.000133 | 0.042676 | 0.000593 | 0.003142 | 0.000107 | 0.025651 |
Lipid Particles | 0.002084 | 0.002292 | 0.000318 | 0.002787 | 0.000936 | 0.001934 | 0.003216 | 0.004580 | 0.001667 | 0.002076 | 0.000509 | 0.003115 |
Mitochondria | 0.001153 | 0.003068 | 0.000542 | 0.025497 | 0.000414 | 0.005391 | 0.000267 | 0.009622 | 0.000885 | 0.004472 | 0.000523 | 0.022595 |
Mitotic Spindle | 0.003312 | 0.008510 | 0.008183 | 0.078209 | 0.001104 | 0.021246 | 0.001799 | 0.053725 | 0.002510 | 0.016207 | 0.007762 | 0.073733 |
None | 0.006348 | 0.006012 | 0.007090 | 0.010698 | 0.002764 | 0.003276 | 0.005482 | 0.008613 | 0.005047 | 0.004358 | 0.006984 | 0.010317 |
Nuclear Periphery | 0.000438 | 0.000314 | 0.001080 | 0.000819 | 0.000219 | 0.000252 | 0.001901 | 0.000774 | 0.000358 | 0.000277 | 0.001134 | 0.000811 |
Nuclear Periphery Foci | 0.000346 | 0.001009 | 0.000586 | 0.001340 | 0.000268 | 0.000350 | 0.006548 | 0.001474 | 0.000318 | 0.000611 | 0.000979 | 0.001364 |
Nucleolus | 0.001040 | 0.001456 | 0.000201 | 0.000288 | 0.000438 | 0.001341 | 0.000371 | 0.000142 | 0.000821 | 0.001387 | 0.000212 | 0.000262 |
Nucleus | 0.170549* | 0.091890 | 0.334033* | 0.108143 | 0.163231* | 0.090136 | 0.247184* | 0.142955* | 0.167891* | 0.090830 | 0.328306* | 0.114507 |
Peroxisomes | 0.000978 | 0.006348 | 0.000180 | 0.005038 | 0.001513 | 0.007320 | 0.000291 | 0.015574 | 0.001172 | 0.006935 | 0.000188 | 0.006964 |
Vacuole | 0.038124 | 0.107438 | 0.037109 | 0.125248 | 0.058423 | 0.128044 | 0.267988* | 0.146228 | 0.045496 | 0.119891 | 0.052336 | 0.129083 |
Vacuole Periphery | 0.000984 | 0.001970 | 0.000180 | 0.009453 | 0.000363 | 0.001419 | 0.000194 | 0.001485 | 0.000758 | 0.001637 | 0.000180 | 0.007996 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.76 | -2.60 | -5.81 | -4.23 | -4.35 | -4.40 | -8.56 | -7.34 | -3.18 | -1.18 | -6.83 | -2.99 | -6.47 | -4.52 | -4.56 |
Bud Neck | 0.44 | 7.05 | 4.48 | 3.76 | -2.59 | -7.05 | 4.72 | 2.92 | 10.39 | -3.50 | -4.34 | 7.01 | 4.33 | 8.40 | -2.55 |
Bud Site | -1.24 | 3.25 | -4.99 | -4.46 | -6.91 | -9.54 | -2.70 | -2.66 | 1.22 | -2.22 | -6.91 | 2.00 | -6.24 | -2.26 | -7.16 |
Cell Periphery | 3.42 | 5.56 | 0.73 | -3.13 | -5.81 | -6.08 | -8.15 | -5.05 | -0.18 | 2.70 | 0.55 | 4.30 | -1.29 | -2.04 | -5.84 |
Cytoplasm | 6.36 | 18.18 | 29.74 | 19.08 | 14.96 | 13.48 | 17.73 | 22.70 | 14.76 | 1.09 | 14.37 | 23.64 | 37.22 | 23.31 | 16.28 |
Cytoplasmic Foci | -5.28 | 31.00 | 27.91 | 24.18 | -4.73 | -8.88 | 18.51 | 16.33 | 25.44 | 0.06 | -9.54 | 36.99 | 32.73 | 36.90 | -4.76 |
Eisosomes | 4.01 | 9.61 | 3.85 | -0.63 | -20.10 | -6.18 | -7.63 | -5.87 | 1.53 | 3.91 | -0.28 | 9.89 | 2.02 | 2.86 | -18.78 |
Endoplasmic Reticulum | -1.37 | -9.57 | -12.19 | -11.90 | -4.89 | -3.93 | -9.62 | -11.16 | -10.69 | 2.77 | -0.93 | -14.76 | -17.03 | -17.50 | -4.98 |
Endosome | -10.32 | 11.14 | -1.89 | 6.71 | -4.32 | -13.01 | -2.67 | -0.55 | 8.46 | 0.55 | -16.63 | 10.67 | -2.03 | 10.17 | -4.48 |
Golgi | -3.31 | 3.31 | -2.76 | -0.50 | -3.58 | -5.68 | 3.65 | -1.64 | -0.41 | -1.69 | -5.94 | 3.69 | -3.34 | -0.58 | -3.96 |
Lipid Particles | -0.62 | 6.32 | 1.04 | 1.63 | -4.47 | -6.15 | -5.54 | -2.30 | 0.46 | 1.54 | -2.00 | 6.35 | -0.15 | 1.42 | -4.73 |
Mitochondria | -1.85 | 5.62 | -2.39 | -1.02 | -2.80 | -4.40 | 3.33 | -1.57 | 3.03 | -1.82 | -4.49 | 4.61 | -2.64 | 0.17 | -2.93 |
Mitotic Spindle | -2.45 | -3.64 | -7.29 | -5.49 | -5.56 | -7.06 | -1.45 | -3.28 | -0.44 | -2.49 | -7.04 | -4.62 | -8.19 | -3.60 | -5.94 |
None | 0.62 | -0.68 | -1.78 | -1.81 | -1.61 | -0.60 | -1.51 | -5.07 | -4.03 | -3.60 | 1.25 | -3.32 | -4.26 | -4.63 | -2.24 |
Nuclear Periphery | 0.44 | -6.35 | -9.03 | -21.29 | -6.44 | -3.14 | -13.57 | -12.05 | -11.73 | -1.11 | 0.41 | -10.53 | -13.84 | -25.10 | -7.03 |
Nuclear Periphery Foci | -3.18 | -3.25 | -6.98 | -1.47 | -5.84 | -2.60 | -8.51 | -7.95 | -7.49 | 5.61 | -3.00 | -7.55 | -10.34 | -7.27 | -3.61 |
Nucleolus | -0.56 | 3.68 | 3.56 | 6.31 | -0.80 | -3.28 | 2.31 | 1.99 | 3.84 | -0.51 | -1.70 | 3.92 | 3.80 | 6.02 | -0.83 |
Nucleus | 13.94 | -33.41 | -6.78 | -17.24 | 21.26 | 12.64 | -5.10 | -7.24 | -12.32 | -0.81 | 19.63 | -35.65 | -9.59 | -22.22 | 20.02 |
Peroxisomes | -6.66 | 8.31 | 0.13 | 6.13 | -2.18 | -6.42 | 3.26 | -0.12 | 4.15 | -1.40 | -9.64 | 6.41 | 0.29 | 8.59 | -2.61 |
Vacuole | -16.11 | -9.31 | -31.46 | -19.77 | -27.47 | -13.97 | -14.61 | -15.57 | -11.56 | 0.93 | -23.18 | -11.40 | -34.46 | -22.81 | -27.62 |
Vacuole Periphery | -1.84 | 3.84 | -1.01 | 0.46 | -2.83 | -5.44 | 1.65 | 0.81 | 7.12 | -1.13 | -3.42 | 4.13 | -1.25 | 0.57 | -2.86 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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