Standard name
Human Ortholog
Description Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.95 0.93 0.98 0.95 0.86 0.86 0.69 0.77 0.57 0.66 0.99 1.0 0.99 0.96 0.94 0.88 0.93 0.91 0.84 0.95 0.9 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.12 0 0.23 0.26 0.32 0.5 0.51 0.57 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0
Bud 1 0 0 2 2 0 0 3 0 3 1 0 2 0 1 1 0 0 4 0 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 2 1 1 2 0 1 3 1 2 1 0 0 3 2 4 0 0 0 0 0 0
Cytoplasm 274 122 109 100 87 155 197 178 101 171 83 100 225 223 269 129 113 46 277 115 109 115 169 127
Endoplasmic Reticulum 0 1 1 1 0 0 0 0 0 0 3 1 2 0 0 10 7 2 0 0 1 0 3 5
Endosome 0 0 0 1 1 0 1 0 0 0 1 0 0 0 1 1 3 0 3 0 2 1 2 0
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 0 0 0 1 1
Mitochondria 16 1 3 13 4 38 59 66 73 113 83 81 0 3 0 5 0 1 2 0 1 0 1 0
Nucleus 2 0 0 3 0 3 1 1 0 0 1 1 0 0 0 0 0 3 0 0 0 0 0 0
Nuclear Periphery 2 1 2 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0
Nucleolus 1 0 1 0 0 0 1 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 3 1 1 1 0 0 7 3 1 6 1 3 0 0 0 2 6 2 1 6 3 0 0 0
Unique Cell Count 287 124 115 108 89 164 228 208 147 223 145 151 228 224 271 135 120 52 299 127 131 121 188 138
Labelled Cell Count 300 127 118 125 95 197 269 251 176 297 174 189 231 227 272 154 132 58 299 127 131 121 188 138


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.8 5.4 4.9 5.3 4.6 4.2 4.1 4.5 3.9 4.1 3.8 5.8 5.4 5.6 7.1 9.1 8.8 6.8 7.4 8.7
Std Deviation (1e-4) 0.6 1.2 1.4 1.3 1.7 1.4 1.6 1.0 1.9 1.5 1.4 1.5 0.9 1.1 1.1 1.9 3.3 2.5 1.5 1.6 1.8
Intensity Change (Log2) – – – -0.13 -0.03 -0.23 -0.37 -0.39 -0.27 -0.48 -0.41 -0.51 0.09 0.01 0.05 0.4 0.75 0.71 0.33 0.44 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 1.1 -0.1 -2.4 -2.5 -5.3 -4.2 -6.8 -5.6 2.1 2.9 2.8 0.3 -0.2 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0007 1.5515 1.1727 1.0887 1.0047 1.4475 2.1143 2.738 2.3619 2.1799 2.1918 2.0448 3.2482 3.0783 2.8384 2.9202 2.4655 2.9749
Actin 0.0171 0.0003 0.0074 0.0009 0.0402 0.0047 0.0457 0.0234 0.0227 0.0669 0.035 0.0007 0.1114 0.0002 0.0442 0.0006 0.0002 0.0176
Bud 0.0002 0.003 0.0021 0.0001 0.0001 0.0007 0.001 0.0021 0.0034 0.0003 0.003 0.0004 0.0011 0 0.0008 0.0001 0 0.0001
Bud Neck 0.0031 0.0002 0.0008 0.0002 0.0012 0.0027 0.0259 0.0002 0.0023 0.0002 0.0016 0.0009 0.0064 0.0001 0.0012 0.0001 0.0001 0.0004
Bud Periphery 0.0004 0.0009 0.0034 0.0001 0.0001 0.0009 0.0019 0.0015 0.0064 0.0003 0.0049 0.0005 0.0018 0 0.0011 0 0 0.0001
Bud Site 0.0011 0.0033 0.0082 0.0002 0.0003 0.0018 0.0099 0.0132 0.0112 0.0009 0.0117 0.0001 0.0053 0.0003 0.0024 0.0001 0 0.0001
Cell Periphery 0.0002 0.0003 0.0003 0.0001 0.0001 0.0002 0.0014 0.0002 0.0005 0.0001 0.0002 0.0001 0.0005 0.0002 0.0003 0 0 0.0001
Cytoplasm 0.5129 0.6422 0.6047 0.6515 0.6055 0.6778 0.3255 0.4779 0.4926 0.5156 0.599 0.6729 0.4054 0.7433 0.5323 0.6878 0.675 0.5949
Cytoplasmic Foci 0.0227 0.0072 0.0126 0.0109 0.0061 0.0124 0.0441 0.0083 0.0189 0.0052 0.0523 0.0084 0.0286 0.0031 0.0139 0.0131 0.0018 0.0071
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0007 0.0001 0.0005 0.0001 0.0002 0.0001 0.0003 0 0.0008 0 0.0009 0 0 0.0001
Endoplasmic Reticulum 0.0041 0.0043 0.0033 0.0057 0.0027 0.0016 0.0081 0.0021 0.0033 0.0016 0.0016 0.0027 0.0061 0.0018 0.0029 0.0019 0.0008 0.0023
Endosome 0.0087 0.0039 0.0053 0.0067 0.0017 0.0043 0.0471 0.0026 0.0131 0.0016 0.0173 0.0039 0.0196 0.0004 0.0071 0.0144 0.0003 0.0018
Golgi 0.0041 0.0003 0.0017 0.0004 0.0016 0.0016 0.0245 0.003 0.0065 0.002 0.0162 0.0001 0.0124 0.0001 0.0047 0.0006 0 0.0008
Lipid Particles 0.0038 0.0005 0.0062 0.0005 0.0026 0.0039 0.0186 0.0016 0.0072 0.0003 0.02 0.0001 0.0133 0.0001 0.0081 0.0004 0 0.0002
Mitochondria 0.0056 0.0002 0.0079 0.0002 0.0012 0.001 0.0084 0.0027 0.0024 0.0005 0.0081 0.0001 0.0046 0.0001 0.0088 0.0002 0.0001 0.0002
None 0.3925 0.3268 0.3182 0.317 0.318 0.2719 0.3993 0.4489 0.385 0.4018 0.1966 0.3061 0.3447 0.2481 0.3365 0.2419 0.3206 0.3712
Nuclear Periphery 0.0016 0.0008 0.001 0.0013 0.0011 0.0017 0.004 0.0003 0.0024 0.0004 0.0008 0.0003 0.0036 0.0003 0.0073 0.0006 0.0001 0.0007
Nucleolus 0.0003 0.0002 0.0003 0.0001 0.0001 0.0018 0.0022 0.0001 0.0018 0 0.0003 0.0001 0.0008 0 0.0027 0 0 0
Nucleus 0.0029 0.0021 0.0019 0.0017 0.0024 0.0031 0.0028 0.0007 0.0049 0.0005 0.001 0.0009 0.0023 0.0011 0.0069 0.0011 0.0005 0.0005
Peroxisomes 0.0147 0.0001 0.0114 0.0002 0.0119 0.003 0.0197 0.01 0.0049 0.0007 0.0257 0.0001 0.0115 0 0.0089 0.0339 0 0.0009
Punctate Nuclear 0.0017 0.0006 0.0012 0.0009 0.002 0.0036 0.0034 0.0004 0.0039 0.0002 0.003 0.0009 0.0174 0.0003 0.0069 0.0005 0.0001 0.0005
Vacuole 0.0015 0.0025 0.0017 0.0009 0.0004 0.0011 0.0048 0.0005 0.0056 0.0007 0.0011 0.0005 0.0016 0.0005 0.0014 0.0019 0.0001 0.0003
Vacuole Periphery 0.0006 0.0002 0.0003 0.0002 0.0001 0.0002 0.0013 0.0001 0.0008 0.0001 0.0003 0.0001 0.0007 0 0.0008 0.0006 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.758 17.4461 12.4479 13.3524 19.4641 9.0024 15.9436 11.9056 13.3854 12.7701
Translational Efficiency 0.6636 0.7475 0.814 0.6741 0.4981 0.7981 0.7986 1.0311 0.67 0.8071

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Msg5

Msg5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msg5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available