Standard name | |
---|---|
Human Ortholog | |
Description | rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 2 | – | 4 | 6 | 9 | 8 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 5 | 7 | 1 | 7 | 6 | 14 | – | 5 | 2 | 3 | 4 | 5 | 12 | 8 | 5 | 9 | 12 | 0 | 1 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 3 | 2 | 1 | 0 | 7 | 15 | 15 | – | 6 | 10 | 4 | 7 | 1 | 2 | 4 | 0 | 2 | 0 | 1 | 2 | 1 | 0 | 1 | 3 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 53 | 44 | 2 | 23 | 10 | 34 | 26 | – | 28 | 25 | 57 | 22 | 22 | 16 | 9 | 30 | 32 | 13 | 3 | 6 | 2 | 1 | 3 | 7 |
Nucleus | 179 | 201 | 56 | 4 | 18 | 12 | 18 | – | 22 | 27 | 23 | 29 | 10 | 5 | 13 | 37 | 30 | 28 | 97 | 148 | 41 | 5 | 7 | 3 |
Nuclear Periphery | 3 | 0 | 2 | 0 | 1 | 1 | 0 | – | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 18 | 9 | 5 | 1 | 1 | 0 |
Nucleolus | 283 | 275 | 96 | 93 | 194 | 212 | 256 | – | 173 | 182 | 183 | 243 | 74 | 97 | 112 | 219 | 178 | 190 | 347 | 327 | 135 | 28 | 37 | 57 |
Peroxisomes | 2 | 1 | 0 | 1 | 5 | 6 | 6 | – | 0 | 1 | 1 | 1 | 1 | 3 | 3 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | 1 | 5 | 11 | 29 | 34 | – | 26 | 37 | 19 | 35 | 4 | 4 | 4 | 2 | 1 | 0 | 10 | 5 | 2 | 0 | 3 | 7 |
Vac/Vac Membrane | 13 | 21 | 11 | 1 | 6 | 4 | 2 | – | 1 | 5 | 0 | 2 | 0 | 0 | 3 | 9 | 6 | 7 | 18 | 34 | 15 | 0 | 3 | 7 |
Unique Cell Count | 454 | 471 | 163 | 98 | 228 | 242 | 303 | 215 | 233 | 218 | 283 | 87 | 116 | 134 | 264 | 218 | 234 | 501 | 543 | 209 | 43 | 64 | 94 | |
Labelled Cell Count | 538 | 552 | 176 | 130 | 260 | 319 | 374 | 274 | 297 | 302 | 354 | 118 | 139 | 158 | 303 | 264 | 252 | 501 | 543 | 209 | 43 | 64 | 94 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.6 | 34.0 | 25.0 | 19.6 | 19.1 | 15.4 | 14.7 | – | 11.9 | 11.4 | 11.7 | 12.3 | 23.3 | 24.8 | 22.6 | 49.6 | 49.0 | 46.8 | – | 22.8 | 23.9 |
Std Deviation (1e-4) | 6.4 | 10.3 | 9.1 | 6.6 | 5.0 | 5.4 | 4.4 | – | 3.5 | 3.3 | 3.6 | 4.2 | 10.6 | 12.4 | 8.6 | 21.3 | 22.4 | 21.6 | – | 8.7 | 11.1 |
Intensity Change (Log2) | – | – | – | -0.35 | -0.39 | -0.7 | -0.76 | – | -1.07 | -1.13 | -1.1 | -1.03 | -0.1 | -0.01 | -0.14 | 0.99 | 0.97 | 0.91 | – | -0.13 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -0.6 | -1.0 | 0.2 | – | -1.1 | 0 | 0 | 0 | 0 | 2.0 | 0.7 | 0 | -0.1 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 2.8 | 2.5 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.9 | 1.8 | 4.4 | 3.2 | – | 4.2 | 3.7 | 6.7 | 3.0 | 6.2 | 4.2 | 0 | 3.9 | 4.6 | 2.2 |
Nucleus | -5.6 | -6.6 | -7.8 | -8.0 | – | -5.7 | -5.5 | -5.7 | -6.2 | -3.9 | -6.0 | -5.0 | -4.9 | -4.8 | -5.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 6.3 | 5.8 | 6.6 | 6.1 | – | 4.6 | 4.1 | 5.5 | 6.4 | 4.2 | 4.4 | 4.6 | 5.5 | 4.9 | 4.9 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2.4 | 4.3 | 4.1 | – | 4.3 | 5.1 | 3.5 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -2.0 | -2.7 | 0 | – | -3.5 | -2.3 | 0 | 0 | 0 | 0 | -1.8 | -1.6 | -1.9 | -1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.1368 | 23.9008 | 22.332 | 20.1334 | 21.8043 | 20.9351 | 18.6935 | 24.1558 | 20.0672 | 14.5669 | 15.9457 | 20.9912 | 23.5311 | 28.5083 | 24.915 | 25.103 | 23.6141 | 25.3459 |
Actin | 0.0154 | 0 | 0.0001 | 0.0009 | 0.0046 | 0.0001 | 0.0239 | 0 | 0.0111 | 0.0264 | 0.0365 | 0.0009 | 0.0213 | 0 | 0.0023 | 0 | 0.0061 | 0 |
Bud | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0 | 0.0045 | 0.0049 | 0.0008 | 0.0001 | 0.0017 | 0 | 0.0038 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0003 | 0.0004 | 0 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 |
Bud Site | 0.0027 | 0.0002 | 0.0005 | 0.0001 | 0.0038 | 0.0001 | 0.0003 | 0 | 0.0035 | 0.0017 | 0.0029 | 0.0002 | 0.0014 | 0 | 0.0023 | 0 | 0.0003 | 0 |
Cell Periphery | 0.0005 | 0.0003 | 0.0002 | 0.0008 | 0.0006 | 0.0009 | 0.0008 | 0.0001 | 0.0003 | 0.0006 | 0.0012 | 0.0007 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0007 |
Cytoplasm | 0.0018 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 | 0.0005 | 0 | 0.0027 | 0.032 | 0.0004 | 0.0001 | 0.0018 | 0 | 0 | 0 | 0.0004 | 0 |
Cytoplasmic Foci | 0.0115 | 0.0004 | 0.0074 | 0.0015 | 0.0004 | 0.0003 | 0.0134 | 0 | 0.0178 | 0.0291 | 0.0166 | 0.0011 | 0.0185 | 0 | 0.0003 | 0 | 0.0112 | 0.0002 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0016 | 0 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0003 | 0 | 0.0009 | 0.0017 | 0.0005 | 0.0001 | 0.0011 | 0 | 0 | 0 | 0.0003 | 0 |
Endosome | 0.0073 | 0.0001 | 0.0007 | 0.0035 | 0.0009 | 0.0002 | 0.006 | 0 | 0.0057 | 0.0111 | 0.0052 | 0.0004 | 0.006 | 0 | 0.0001 | 0 | 0.011 | 0 |
Golgi | 0.0032 | 0 | 0.0004 | 0.001 | 0.0012 | 0.0001 | 0.0036 | 0 | 0.0044 | 0.0058 | 0.0056 | 0.0005 | 0.0026 | 0 | 0.0002 | 0 | 0.0051 | 0.0002 |
Lipid Particles | 0.0127 | 0.0016 | 0.0031 | 0.0056 | 0.0031 | 0.0078 | 0.0149 | 0.0001 | 0.0134 | 0.0189 | 0.0257 | 0.0094 | 0.0078 | 0.0001 | 0.0016 | 0.0001 | 0.0286 | 0.0123 |
Mitochondria | 0.0058 | 0.0006 | 0.0006 | 0.0022 | 0.0263 | 0.0188 | 0.01 | 0.0006 | 0.0067 | 0.0051 | 0.0295 | 0.0247 | 0.0021 | 0.0006 | 0.0007 | 0.0004 | 0.0026 | 0.0107 |
None | 0.0015 | 0 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0078 | 0 | 0.0078 | 0.0226 | 0.0009 | 0.0003 | 0.0024 | 0 | 0 | 0 | 0.001 | 0 |
Nuclear Periphery | 0.0161 | 0.0224 | 0.0024 | 0.0205 | 0.0128 | 0.0159 | 0.0021 | 0.0014 | 0.0027 | 0.0036 | 0.067 | 0.0077 | 0.0082 | 0.0016 | 0.0095 | 0.0001 | 0.0033 | 0.0139 |
Nucleolus | 0.7904 | 0.8221 | 0.8426 | 0.8119 | 0.7507 | 0.7922 | 0.7827 | 0.8582 | 0.8117 | 0.706 | 0.6565 | 0.8277 | 0.8181 | 0.8753 | 0.8977 | 0.926 | 0.6986 | 0.862 |
Nucleus | 0.0907 | 0.1453 | 0.1242 | 0.1266 | 0.1778 | 0.1365 | 0.1159 | 0.1383 | 0.0908 | 0.1047 | 0.1184 | 0.1026 | 0.0789 | 0.1171 | 0.0785 | 0.0728 | 0.197 | 0.0809 |
Peroxisomes | 0.0151 | 0 | 0.0053 | 0.0002 | 0.001 | 0.0001 | 0.0068 | 0 | 0.0068 | 0.0101 | 0.003 | 0.0005 | 0.0037 | 0 | 0.0002 | 0 | 0.0176 | 0.0001 |
Punctate Nuclear | 0.0088 | 0.0006 | 0.0098 | 0.0033 | 0.0012 | 0.0008 | 0.0054 | 0.0003 | 0.0054 | 0.0082 | 0.0014 | 0.002 | 0.0207 | 0.0002 | 0.0002 | 0.0002 | 0.0131 | 0.0011 |
Vacuole | 0.0021 | 0.0018 | 0.0007 | 0.0032 | 0.0018 | 0.0059 | 0.0015 | 0.0002 | 0.0012 | 0.0032 | 0.0034 | 0.0017 | 0.001 | 0.0019 | 0.0002 | 0.0001 | 0.0008 | 0.0012 |
Vacuole Periphery | 0.0118 | 0.0036 | 0.0012 | 0.0163 | 0.0116 | 0.0196 | 0.0034 | 0.0008 | 0.0019 | 0.0034 | 0.0223 | 0.0187 | 0.0016 | 0.0027 | 0.0015 | 0.0002 | 0.0023 | 0.0163 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 114.9751 | 113.1385 | 111.9382 | 80.5007 | 112.862 | 92.6418 | 82.8418 | 65.609 | 63.0076 | 81.4388 |
Translational Efficiency | 1.3295 | 1.3402 | 1.0148 | 1.5227 | 0.9838 | 1.0758 | 1.2759 | 1.1898 | 1.0724 | 1.1445 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1983 | 969 | 259 | 2234 | 1835 | 1926 | 1714 | 125 | 3818 | 2895 | 1973 | 2359 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1411.51 | 1931.94 | 2625.89 | 3433.93 | 1785.47 | 1895.18 | 2395.60 | 3128.68 | 1591.24 | 1907.48 | 2425.83 | 3417.76 |
Standard Deviation | 300.97 | 568.20 | 490.96 | 985.61 | 427.20 | 487.75 | 566.91 | 803.55 | 411.91 | 516.37 | 562.93 | 979.20 |
Intensity Change Log 2 | — | 0.452811 | 0.895567 | 1.282621 | — | 0.086031 | 0.424083 | 0.809250 | — | 0.259549 | 0.651406 | 1.037560 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000734 | 0.000736 | 0.000477 | 0.001456 | 0.000691 | 0.000789 | 0.000843 | 0.001122 | 0.000714 | 0.000771 | 0.000795 | 0.001438 |
Bud Neck | 0.002826 | 0.000975 | 0.002054 | 0.005113 | 0.000950 | 0.001999 | 0.003140 | 0.006568 | 0.001924 | 0.001656 | 0.002997 | 0.005190 |
Bud Site | 0.001874 | 0.000171 | 0.000388 | 0.000828 | 0.000141 | 0.000329 | 0.000381 | 0.000817 | 0.001041 | 0.000276 | 0.000382 | 0.000827 |
Cell Periphery | 0.000279 | 0.000023 | 0.000010 | 0.000134 | 0.000015 | 0.000140 | 0.000026 | 0.000062 | 0.000152 | 0.000101 | 0.000024 | 0.000130 |
Cytoplasm | 0.000684 | 0.000166 | 0.000016 | 0.000264 | 0.000054 | 0.000393 | 0.000071 | 0.000126 | 0.000381 | 0.000317 | 0.000064 | 0.000257 |
Cytoplasmic Foci | 0.025665 | 0.002754 | 0.002958 | 0.004077 | 0.001916 | 0.001785 | 0.001032 | 0.003392 | 0.014251 | 0.002109 | 0.001285 | 0.004041 |
Eisosomes | 0.000650 | 0.000160 | 0.000170 | 0.000264 | 0.000233 | 0.000213 | 0.000175 | 0.000118 | 0.000450 | 0.000195 | 0.000174 | 0.000256 |
Endoplasmic Reticulum | 0.000469 | 0.000134 | 0.000096 | 0.000200 | 0.000124 | 0.000314 | 0.000128 | 0.000168 | 0.000303 | 0.000254 | 0.000124 | 0.000198 |
Endosome | 0.000413 | 0.000279 | 0.000011 | 0.000084 | 0.000013 | 0.000118 | 0.000088 | 0.000069 | 0.000221 | 0.000172 | 0.000078 | 0.000083 |
Golgi | 0.000360 | 0.000160 | 0.000256 | 0.000240 | 0.000076 | 0.000183 | 0.000114 | 0.000295 | 0.000224 | 0.000175 | 0.000133 | 0.000243 |
Lipid Particles | 0.000897 | 0.000059 | 0.000015 | 0.000144 | 0.000036 | 0.000361 | 0.000007 | 0.000048 | 0.000483 | 0.000260 | 0.000008 | 0.000139 |
Mitochondria | 0.005937 | 0.001764 | 0.001804 | 0.005886 | 0.001620 | 0.001689 | 0.002602 | 0.005389 | 0.003862 | 0.001714 | 0.002498 | 0.005860 |
Mitotic Spindle | 0.006857 | 0.000172 | 0.006534 | 0.003549 | 0.001157 | 0.002014 | 0.000859 | 0.006612 | 0.004117 | 0.001398 | 0.001604 | 0.003711 |
None | 0.002213 | 0.000983 | 0.000266 | 0.000955 | 0.000427 | 0.000826 | 0.000559 | 0.000753 | 0.001355 | 0.000879 | 0.000520 | 0.000945 |
Nuclear Periphery | 0.001809 | 0.001293 | 0.001510 | 0.016778 | 0.001960 | 0.001156 | 0.001467 | 0.012819 | 0.001882 | 0.001202 | 0.001473 | 0.016568 |
Nuclear Periphery Foci | 0.003902 | 0.000656 | 0.000564 | 0.006192 | 0.001338 | 0.000608 | 0.000332 | 0.002524 | 0.002669 | 0.000624 | 0.000362 | 0.005997 |
Nucleolus | 0.671034* | 0.627595* | 0.727877* | 0.667628* | 0.695174* | 0.550887* | 0.634425* | 0.662573* | 0.682636* | 0.576563* | 0.646693* | 0.667360* |
Nucleus | 0.146185* | 0.324650* | 0.135885* | 0.231434* | 0.244425* | 0.409751* | 0.321615* | 0.247969* | 0.193401* | 0.381266* | 0.297234* | 0.232310* |
Peroxisomes | 0.121867* | 0.032931 | 0.117945* | 0.020852 | 0.046844 | 0.021724 | 0.029756 | 0.039892 | 0.085809 | 0.025475 | 0.041333 | 0.021861 |
Vacuole | 0.003207 | 0.000880 | 0.000392 | 0.003813 | 0.000473 | 0.001398 | 0.000419 | 0.001220 | 0.001893 | 0.001225 | 0.000416 | 0.003675 |
Vacuole Periphery | 0.002136 | 0.003456 | 0.000771 | 0.030111 | 0.002334 | 0.003324 | 0.001958 | 0.007464 | 0.002231 | 0.003368 | 0.001802 | 0.028911 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.05 | 4.25 | -11.55 | -12.61 | -14.56 | -2.58 | -3.92 | -3.96 | -2.96 | -2.50 | -1.81 | -2.32 | -13.85 | -12.66 | -11.70 |
Bud Neck | 8.71 | 2.17 | -7.68 | -14.68 | -8.80 | -5.38 | -12.83 | -4.10 | -3.43 | -2.76 | 1.51 | -6.39 | -12.10 | -12.44 | -7.89 |
Bud Site | 4.77 | 3.99 | 2.64 | -6.51 | -3.90 | -2.99 | -5.58 | -2.45 | -1.87 | -1.79 | 3.97 | 3.47 | 0.67 | -5.51 | -4.76 |
Cell Periphery | 2.35 | 2.46 | 1.24 | -4.42 | -4.89 | -2.34 | -3.79 | -3.15 | 1.28 | -2.44 | 0.71 | 2.25 | 0.26 | -0.72 | -4.46 |
Cytoplasm | 3.67 | 5.20 | 2.96 | -1.44 | -5.21 | -1.96 | 0.05 | -1.22 | 1.71 | -1.93 | -0.15 | 4.60 | 1.44 | 0.89 | -4.21 |
Cytoplasmic Foci | 10.94 | 11.49 | 11.19 | -1.17 | -0.96 | -0.08 | 2.08 | -1.52 | -1.42 | -2.44 | 10.98 | 13.02 | 8.80 | -2.72 | -5.06 |
Eisosomes | 12.49 | 12.09 | 8.74 | -4.46 | -3.81 | 1.25 | 3.88 | 6.86 | 6.29 | 4.37 | 11.36 | 12.36 | 6.56 | -2.75 | -3.68 |
Endoplasmic Reticulum | 3.51 | 3.94 | 2.98 | -1.84 | -3.65 | -3.20 | -0.54 | -1.36 | 1.83 | -1.23 | 0.68 | 3.75 | 2.15 | 1.37 | -5.00 |
Endosome | 0.49 | 2.73 | 2.19 | 0.72 | -3.95 | -3.21 | -0.99 | -2.09 | 0.72 | 0.02 | 0.43 | 1.43 | 1.69 | 0.93 | -0.16 |
Golgi | 2.32 | 1.63 | 2.19 | -1.18 | 0.98 | -1.80 | -1.65 | -4.22 | -1.58 | -3.31 | 0.88 | 2.59 | -0.66 | -1.42 | -4.75 |
Lipid Particles | 4.86 | 5.20 | 4.17 | -1.18 | -2.01 | -1.32 | 2.04 | -0.75 | 1.24 | -2.09 | 1.21 | 5.36 | 3.23 | 0.70 | -2.17 |
Mitochondria | 6.27 | 6.10 | 0.01 | -11.82 | -11.01 | -0.48 | -5.12 | -4.23 | -4.13 | -3.10 | 6.02 | 3.61 | -4.45 | -13.00 | -9.95 |
Mitotic Spindle | 7.14 | 0.14 | 2.76 | -5.20 | 1.63 | -1.14 | 0.84 | -1.77 | -1.66 | -1.80 | 3.64 | 4.31 | -0.34 | -3.08 | -3.28 |
None | 2.49 | 3.98 | 2.54 | 0.16 | -8.93 | -2.45 | -1.71 | -4.12 | 0.24 | -2.06 | 1.68 | 3.18 | 1.54 | -0.48 | -4.47 |
Nuclear Periphery | 1.03 | 0.40 | -9.99 | -10.23 | -9.06 | 2.04 | 0.99 | -1.90 | -2.05 | -1.99 | 2.18 | 0.96 | -10.24 | -10.71 | -10.28 |
Nuclear Periphery Foci | 5.73 | 5.27 | -3.08 | -11.23 | -9.79 | 2.67 | 4.65 | -1.51 | -2.25 | -2.60 | 6.30 | 7.56 | -6.18 | -11.79 | -12.75 |
Nucleolus | 3.98 | -3.84 | 0.56 | -3.48 | 4.12 | 15.48* | 6.67 | 1.97 | -3.35 | -0.32 | 15.15* | 5.00 | 2.57 | -10.91 | -2.33 |
Nucleus | -17.26 | 0.66 | -12.82 | 8.41 | -7.92 | -17.30 | -8.21 | -0.45 | 5.80 | 2.51 | -26.99 | -13.78 | -6.34 | 18.60 | 7.56 |
Peroxisomes | 22.88* | -0.25 | 29.27* | 5.29 | 13.51 | 11.65 | 7.64 | 1.28 | -2.82 | -1.44 | 26.55* | 16.74 | 28.72 | 2.43 | 11.00 |
Vacuole | 4.95 | 6.40 | -1.14 | -8.96 | -12.18 | -3.08 | 0.51 | -3.05 | 0.36 | -3.11 | 2.03 | 6.12 | -5.10 | -7.11 | -11.91 |
Vacuole Periphery | -2.93 | 6.42 | -16.03 | -14.95 | -16.93 | -1.61 | 1.15 | -2.91 | -2.22 | -3.16 | -2.68 | 1.84 | -16.10 | -15.05 | -16.37 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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