Standard name
Human Ortholog
Description rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 – 0 0 0 0 0.06 0.1 0.06 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0.06 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.09 0 0.23 0 0.14 0.09 – 0.13 0.11 0.26 0.08 0.25 0.14 0.07 0.11 0.15 0.06 0 0 0 0 0.05 0.08
Nucleus 0.39 0.43 0.34 0 0.08 0.05 0.06 – 0.1 0.12 0.11 0.1 0.11 0 0.1 0.14 0.14 0.12 0.19 0.27 0.2 0.13 0.12 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 0.58 0.59 0.95 0.85 0.88 0.84 – 0.8 0.78 0.84 0.86 0.85 0.84 0.84 0.83 0.82 0.81 0.69 0.6 0.65 0.65 0.59 0.62
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.05 0.12 0.11 – 0.12 0.16 0.09 0.12 0.05 0 0 0 0 0 0 0 0 0 0.05 0.08
Vac/Vac Membrane 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 0 – 2 0 0 2 1 0 0 0 0 0 1 2 1 0 1 2
Bud Neck 0 0 0 1 1 0 1 – 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 2 – 4 6 9 8 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 7 1 7 6 14 – 5 2 3 4 5 12 8 5 9 12 0 1 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 1 3 1 0 0 0 0 0 0
Endosome 3 2 1 0 7 15 15 – 6 10 4 7 1 2 4 0 2 0 1 2 1 0 1 3
Golgi 0 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 53 44 2 23 10 34 26 – 28 25 57 22 22 16 9 30 32 13 3 6 2 1 3 7
Nucleus 179 201 56 4 18 12 18 – 22 27 23 29 10 5 13 37 30 28 97 148 41 5 7 3
Nuclear Periphery 3 0 2 0 1 1 0 – 4 2 2 0 0 0 0 0 0 0 18 9 5 1 1 0
Nucleolus 283 275 96 93 194 212 256 – 173 182 183 243 74 97 112 219 178 190 347 327 135 28 37 57
Peroxisomes 2 1 0 1 5 6 6 – 0 1 1 1 1 3 3 0 3 1 0 0 0 0 0 0
SpindlePole 1 2 1 5 11 29 34 – 26 37 19 35 4 4 4 2 1 0 10 5 2 0 3 7
Vac/Vac Membrane 13 21 11 1 6 4 2 – 1 5 0 2 0 0 3 9 6 7 18 34 15 0 3 7
Unique Cell Count 454 471 163 98 228 242 303 215 233 218 283 87 116 134 264 218 234 501 543 209 43 64 94
Labelled Cell Count 538 552 176 130 260 319 374 274 297 302 354 118 139 158 303 264 252 501 543 209 43 64 94


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.6 34.0 25.0 19.6 19.1 15.4 14.7 – 11.9 11.4 11.7 12.3 23.3 24.8 22.6 49.6 49.0 46.8 – 22.8 23.9
Std Deviation (1e-4) 6.4 10.3 9.1 6.6 5.0 5.4 4.4 – 3.5 3.3 3.6 4.2 10.6 12.4 8.6 21.3 22.4 21.6 – 8.7 11.1
Intensity Change (Log2) – – – -0.35 -0.39 -0.7 -0.76 – -1.07 -1.13 -1.1 -1.03 -0.1 -0.01 -0.14 0.99 0.97 0.91 – -0.13 -0.06

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP7000204060WT3HU80HU120HU1600204060WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30204060WT1AF140AF1800204060
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.6 -1.0 0.2 – -1.1 0 0 0 0 2.0 0.7 0 -0.1 0.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 2.8 2.5 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.9 1.8 4.4 3.2 – 4.2 3.7 6.7 3.0 6.2 4.2 0 3.9 4.6 2.2
Nucleus -5.6 -6.6 -7.8 -8.0 – -5.7 -5.5 -5.7 -6.2 -3.9 -6.0 -5.0 -4.9 -4.8 -5.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.3 5.8 6.6 6.1 – 4.6 4.1 5.5 6.4 4.2 4.4 4.6 5.5 4.9 4.9
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.4 4.3 4.1 – 4.3 5.1 3.5 4.4 0 0 0 0 0 0
Vacuole 0 -2.0 -2.7 0 – -3.5 -2.3 0 0 0 0 -1.8 -1.6 -1.9 -1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.1368 23.9008 22.332 20.1334 21.8043 20.9351 18.6935 24.1558 20.0672 14.5669 15.9457 20.9912 23.5311 28.5083 24.915 25.103 23.6141 25.3459
Actin 0.0154 0 0.0001 0.0009 0.0046 0.0001 0.0239 0 0.0111 0.0264 0.0365 0.0009 0.0213 0 0.0023 0 0.0061 0
Bud 0.0002 0.0003 0 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0
Bud Neck 0.0004 0.0001 0.0001 0.0001 0.0005 0.0002 0.0002 0 0.0045 0.0049 0.0008 0.0001 0.0017 0 0.0038 0 0.0001 0.0001
Bud Periphery 0.0003 0.0004 0 0.0003 0.0007 0.0003 0.0002 0 0.0003 0.0002 0.001 0.0003 0.0002 0 0.0002 0 0.0002 0.0001
Bud Site 0.0027 0.0002 0.0005 0.0001 0.0038 0.0001 0.0003 0 0.0035 0.0017 0.0029 0.0002 0.0014 0 0.0023 0 0.0003 0
Cell Periphery 0.0005 0.0003 0.0002 0.0008 0.0006 0.0009 0.0008 0.0001 0.0003 0.0006 0.0012 0.0007 0.0003 0.0004 0.0004 0.0001 0.0003 0.0007
Cytoplasm 0.0018 0.0001 0.0003 0.0004 0 0 0.0005 0 0.0027 0.032 0.0004 0.0001 0.0018 0 0 0 0.0004 0
Cytoplasmic Foci 0.0115 0.0004 0.0074 0.0015 0.0004 0.0003 0.0134 0 0.0178 0.0291 0.0166 0.0011 0.0185 0 0.0003 0 0.0112 0.0002
Eisosomes 0.0002 0 0 0 0.0005 0 0.0002 0 0.0002 0.0004 0.0006 0.0001 0.0002 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0016 0 0.0001 0.0011 0.0001 0 0.0003 0 0.0009 0.0017 0.0005 0.0001 0.0011 0 0 0 0.0003 0
Endosome 0.0073 0.0001 0.0007 0.0035 0.0009 0.0002 0.006 0 0.0057 0.0111 0.0052 0.0004 0.006 0 0.0001 0 0.011 0
Golgi 0.0032 0 0.0004 0.001 0.0012 0.0001 0.0036 0 0.0044 0.0058 0.0056 0.0005 0.0026 0 0.0002 0 0.0051 0.0002
Lipid Particles 0.0127 0.0016 0.0031 0.0056 0.0031 0.0078 0.0149 0.0001 0.0134 0.0189 0.0257 0.0094 0.0078 0.0001 0.0016 0.0001 0.0286 0.0123
Mitochondria 0.0058 0.0006 0.0006 0.0022 0.0263 0.0188 0.01 0.0006 0.0067 0.0051 0.0295 0.0247 0.0021 0.0006 0.0007 0.0004 0.0026 0.0107
None 0.0015 0 0.0004 0.0005 0.0002 0.0001 0.0078 0 0.0078 0.0226 0.0009 0.0003 0.0024 0 0 0 0.001 0
Nuclear Periphery 0.0161 0.0224 0.0024 0.0205 0.0128 0.0159 0.0021 0.0014 0.0027 0.0036 0.067 0.0077 0.0082 0.0016 0.0095 0.0001 0.0033 0.0139
Nucleolus 0.7904 0.8221 0.8426 0.8119 0.7507 0.7922 0.7827 0.8582 0.8117 0.706 0.6565 0.8277 0.8181 0.8753 0.8977 0.926 0.6986 0.862
Nucleus 0.0907 0.1453 0.1242 0.1266 0.1778 0.1365 0.1159 0.1383 0.0908 0.1047 0.1184 0.1026 0.0789 0.1171 0.0785 0.0728 0.197 0.0809
Peroxisomes 0.0151 0 0.0053 0.0002 0.001 0.0001 0.0068 0 0.0068 0.0101 0.003 0.0005 0.0037 0 0.0002 0 0.0176 0.0001
Punctate Nuclear 0.0088 0.0006 0.0098 0.0033 0.0012 0.0008 0.0054 0.0003 0.0054 0.0082 0.0014 0.002 0.0207 0.0002 0.0002 0.0002 0.0131 0.0011
Vacuole 0.0021 0.0018 0.0007 0.0032 0.0018 0.0059 0.0015 0.0002 0.0012 0.0032 0.0034 0.0017 0.001 0.0019 0.0002 0.0001 0.0008 0.0012
Vacuole Periphery 0.0118 0.0036 0.0012 0.0163 0.0116 0.0196 0.0034 0.0008 0.0019 0.0034 0.0223 0.0187 0.0016 0.0027 0.0015 0.0002 0.0023 0.0163

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.9751 113.1385 111.9382 80.5007 112.862 92.6418 82.8418 65.609 63.0076 81.4388
Translational Efficiency 1.3295 1.3402 1.0148 1.5227 0.9838 1.0758 1.2759 1.1898 1.0724 1.1445

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1983 969 259 2234 1835 1926 1714 125 3818 2895 1973 2359

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1411.51 1931.94 2625.89 3433.93 1785.47 1895.18 2395.60 3128.68 1591.24 1907.48 2425.83 3417.76
Standard Deviation 300.97 568.20 490.96 985.61 427.20 487.75 566.91 803.55 411.91 516.37 562.93 979.20
Intensity Change Log 2 — 0.452811 0.895567 1.282621 — 0.086031 0.424083 0.809250 — 0.259549 0.651406 1.037560

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000734 0.000736 0.000477 0.001456 0.000691 0.000789 0.000843 0.001122 0.000714 0.000771 0.000795 0.001438
Bud Neck 0.002826 0.000975 0.002054 0.005113 0.000950 0.001999 0.003140 0.006568 0.001924 0.001656 0.002997 0.005190
Bud Site 0.001874 0.000171 0.000388 0.000828 0.000141 0.000329 0.000381 0.000817 0.001041 0.000276 0.000382 0.000827
Cell Periphery 0.000279 0.000023 0.000010 0.000134 0.000015 0.000140 0.000026 0.000062 0.000152 0.000101 0.000024 0.000130
Cytoplasm 0.000684 0.000166 0.000016 0.000264 0.000054 0.000393 0.000071 0.000126 0.000381 0.000317 0.000064 0.000257
Cytoplasmic Foci 0.025665 0.002754 0.002958 0.004077 0.001916 0.001785 0.001032 0.003392 0.014251 0.002109 0.001285 0.004041
Eisosomes 0.000650 0.000160 0.000170 0.000264 0.000233 0.000213 0.000175 0.000118 0.000450 0.000195 0.000174 0.000256
Endoplasmic Reticulum 0.000469 0.000134 0.000096 0.000200 0.000124 0.000314 0.000128 0.000168 0.000303 0.000254 0.000124 0.000198
Endosome 0.000413 0.000279 0.000011 0.000084 0.000013 0.000118 0.000088 0.000069 0.000221 0.000172 0.000078 0.000083
Golgi 0.000360 0.000160 0.000256 0.000240 0.000076 0.000183 0.000114 0.000295 0.000224 0.000175 0.000133 0.000243
Lipid Particles 0.000897 0.000059 0.000015 0.000144 0.000036 0.000361 0.000007 0.000048 0.000483 0.000260 0.000008 0.000139
Mitochondria 0.005937 0.001764 0.001804 0.005886 0.001620 0.001689 0.002602 0.005389 0.003862 0.001714 0.002498 0.005860
Mitotic Spindle 0.006857 0.000172 0.006534 0.003549 0.001157 0.002014 0.000859 0.006612 0.004117 0.001398 0.001604 0.003711
None 0.002213 0.000983 0.000266 0.000955 0.000427 0.000826 0.000559 0.000753 0.001355 0.000879 0.000520 0.000945
Nuclear Periphery 0.001809 0.001293 0.001510 0.016778 0.001960 0.001156 0.001467 0.012819 0.001882 0.001202 0.001473 0.016568
Nuclear Periphery Foci 0.003902 0.000656 0.000564 0.006192 0.001338 0.000608 0.000332 0.002524 0.002669 0.000624 0.000362 0.005997
Nucleolus 0.671034 0.627595 0.727877 0.667628 0.695174 0.550887 0.634425 0.662573 0.682636 0.576563 0.646693 0.667360
Nucleus 0.146185 0.324650 0.135885 0.231434 0.244425 0.409751 0.321615 0.247969 0.193401 0.381266 0.297234 0.232310
Peroxisomes 0.121867 0.032931 0.117945 0.020852 0.046844 0.021724 0.029756 0.039892 0.085809 0.025475 0.041333 0.021861
Vacuole 0.003207 0.000880 0.000392 0.003813 0.000473 0.001398 0.000419 0.001220 0.001893 0.001225 0.000416 0.003675
Vacuole Periphery 0.002136 0.003456 0.000771 0.030111 0.002334 0.003324 0.001958 0.007464 0.002231 0.003368 0.001802 0.028911

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.05 4.25 -11.55 -12.61 -14.56 -2.58 -3.92 -3.96 -2.96 -2.50 -1.81 -2.32 -13.85 -12.66 -11.70
Bud Neck 8.71 2.17 -7.68 -14.68 -8.80 -5.38 -12.83 -4.10 -3.43 -2.76 1.51 -6.39 -12.10 -12.44 -7.89
Bud Site 4.77 3.99 2.64 -6.51 -3.90 -2.99 -5.58 -2.45 -1.87 -1.79 3.97 3.47 0.67 -5.51 -4.76
Cell Periphery 2.35 2.46 1.24 -4.42 -4.89 -2.34 -3.79 -3.15 1.28 -2.44 0.71 2.25 0.26 -0.72 -4.46
Cytoplasm 3.67 5.20 2.96 -1.44 -5.21 -1.96 0.05 -1.22 1.71 -1.93 -0.15 4.60 1.44 0.89 -4.21
Cytoplasmic Foci 10.94 11.49 11.19 -1.17 -0.96 -0.08 2.08 -1.52 -1.42 -2.44 10.98 13.02 8.80 -2.72 -5.06
Eisosomes 12.49 12.09 8.74 -4.46 -3.81 1.25 3.88 6.86 6.29 4.37 11.36 12.36 6.56 -2.75 -3.68
Endoplasmic Reticulum 3.51 3.94 2.98 -1.84 -3.65 -3.20 -0.54 -1.36 1.83 -1.23 0.68 3.75 2.15 1.37 -5.00
Endosome 0.49 2.73 2.19 0.72 -3.95 -3.21 -0.99 -2.09 0.72 0.02 0.43 1.43 1.69 0.93 -0.16
Golgi 2.32 1.63 2.19 -1.18 0.98 -1.80 -1.65 -4.22 -1.58 -3.31 0.88 2.59 -0.66 -1.42 -4.75
Lipid Particles 4.86 5.20 4.17 -1.18 -2.01 -1.32 2.04 -0.75 1.24 -2.09 1.21 5.36 3.23 0.70 -2.17
Mitochondria 6.27 6.10 0.01 -11.82 -11.01 -0.48 -5.12 -4.23 -4.13 -3.10 6.02 3.61 -4.45 -13.00 -9.95
Mitotic Spindle 7.14 0.14 2.76 -5.20 1.63 -1.14 0.84 -1.77 -1.66 -1.80 3.64 4.31 -0.34 -3.08 -3.28
None 2.49 3.98 2.54 0.16 -8.93 -2.45 -1.71 -4.12 0.24 -2.06 1.68 3.18 1.54 -0.48 -4.47
Nuclear Periphery 1.03 0.40 -9.99 -10.23 -9.06 2.04 0.99 -1.90 -2.05 -1.99 2.18 0.96 -10.24 -10.71 -10.28
Nuclear Periphery Foci 5.73 5.27 -3.08 -11.23 -9.79 2.67 4.65 -1.51 -2.25 -2.60 6.30 7.56 -6.18 -11.79 -12.75
Nucleolus 3.98 -3.84 0.56 -3.48 4.12 15.48 6.67 1.97 -3.35 -0.32 15.15 5.00 2.57 -10.91 -2.33
Nucleus -17.26 0.66 -12.82 8.41 -7.92 -17.30 -8.21 -0.45 5.80 2.51 -26.99 -13.78 -6.34 18.60 7.56
Peroxisomes 22.88 -0.25 29.27 5.29 13.51 11.65 7.64 1.28 -2.82 -1.44 26.55 16.74 28.72 2.43 11.00
Vacuole 4.95 6.40 -1.14 -8.96 -12.18 -3.08 0.51 -3.05 0.36 -3.11 2.03 6.12 -5.10 -7.11 -11.91
Vacuole Periphery -2.93 6.42 -16.03 -14.95 -16.93 -1.61 1.15 -2.91 -2.22 -3.16 -2.68 1.84 -16.10 -15.05 -16.37
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer
Localization
Cell Percentages nucleolus (50%), nucleus (9%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nop2

Nop2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available