Standard name | |
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Human Ortholog | |
Description | Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 4 | 0 |
Bud | 0 | 4 | 0 | 1 | 2 | 3 | 1 | – | 2 | 4 | 7 | 8 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 8 | 2 | 4 | 11 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 5 | 2 | 1 | – | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 1 | 1 | 2 | 5 |
Bud Site | 0 | 4 | 0 | 0 | 0 | 5 | 23 | – | 17 | 25 | 24 | 21 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 18 | 56 | 15 | 13 | 22 | 34 | 40 | – | 24 | 19 | 21 | 18 | 20 | 43 | 53 | 37 | 33 | 27 | 2 | 26 | 4 | 2 | 6 | 7 |
Endoplasmic Reticulum | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 12 | 16 | 4 | 1 | 0 | 0 | 2 | 3 | 3 |
Endosome | 0 | 0 | 2 | 4 | 4 | 11 | 10 | – | 3 | 4 | 3 | 6 | 0 | 7 | 6 | 5 | 9 | 4 | 4 | 1 | 3 | 1 | 10 | 13 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 21 | 216 | 8 | 29 | 19 | 109 | 158 | – | 253 | 264 | 211 | 155 | 48 | 22 | 33 | 32 | 13 | 32 | 4 | 7 | 9 | 1 | 10 | 16 |
Nucleus | 242 | 220 | 427 | 343 | 441 | 392 | 557 | – | 456 | 485 | 259 | 404 | 425 | 434 | 354 | 289 | 310 | 249 | 235 | 246 | 404 | 212 | 289 | 200 |
Nuclear Periphery | 1 | 2 | 0 | 2 | 0 | 0 | 0 | – | 3 | 6 | 2 | 5 | 2 | 5 | 3 | 1 | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 3 |
Nucleolus | 9 | 6 | 0 | 0 | 1 | 5 | 6 | – | 17 | 26 | 25 | 19 | 9 | 16 | 16 | 5 | 5 | 1 | 15 | 3 | 10 | 13 | 32 | 24 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 5 | 19 | 5 | 17 | 13 | 25 | – | 10 | 13 | 10 | 17 | 0 | 8 | 3 | 2 | 5 | 0 | 3 | 12 | 5 | 6 | 12 | 16 |
Vac/Vac Membrane | 7 | 12 | 7 | 5 | 14 | 19 | 22 | – | 12 | 15 | 8 | 12 | 6 | 14 | 14 | 27 | 40 | 29 | 12 | 70 | 42 | 35 | 99 | 92 |
Unique Cell Count | 279 | 363 | 454 | 370 | 497 | 507 | 690 | 599 | 641 | 381 | 513 | 466 | 513 | 453 | 362 | 394 | 308 | 295 | 389 | 491 | 285 | 496 | 405 | |
Labelled Cell Count | 303 | 525 | 478 | 403 | 526 | 593 | 843 | 797 | 863 | 570 | 668 | 510 | 550 | 484 | 412 | 435 | 348 | 295 | 389 | 491 | 285 | 496 | 405 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 3.5 | 5.9 | 5.4 | 5.8 | 4.7 | 5.0 | – | 4.5 | 4.5 | 4.1 | 4.9 | 5.3 | 5.3 | 5.6 | 7.8 | 8.0 | 8.0 | 4.9 | 4.9 | 5.1 |
Std Deviation (1e-4) | 1.1 | 0.7 | 1.1 | 1.1 | 1.2 | 1.0 | 1.3 | – | 1.0 | 1.1 | 1.2 | 1.0 | 1.1 | 1.1 | 7.0 | 1.9 | 1.9 | 2.0 | 1.0 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.02 | -0.34 | -0.23 | – | -0.39 | -0.38 | -0.51 | -0.28 | -0.16 | -0.16 | -0.08 | 0.4 | 0.44 | 0.45 | -0.27 | -0.27 | -0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.2 | 5.5 | 5.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6962 | 3.5652 | 3.6613 | 3.5041 | 3.1036 | 3.5017 | 1.1254 | 1.3544 | 1.3582 | 1.2168 | 1.4002 | 1.1061 | 1.3706 | 1.6548 | 1.5864 | 1.4466 | 1.3971 | 1.389 |
Actin | 0.024 | 0 | 0 | 0 | 0.0004 | 0 | 0.0045 | 0 | 0 | 0 | 0.0238 | 0.0006 | 0.0014 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0016 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0.0009 | 0.0013 | 0.0002 | 0.0001 | 0.0007 | 0.0008 | 0.0008 | 0.0004 | 0.0001 | 0 | 0 | 0.0004 | 0.0006 |
Bud Periphery | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0011 | 0.0004 | 0 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 |
Bud Site | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0035 | 0.0011 | 0.0001 | 0.0002 | 0.0016 | 0.0001 | 0.0007 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0042 | 0.0004 | 0.0001 | 0.0006 | 0.001 | 0 | 0.0011 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0012 | 0.0004 | 0.0001 | 0 | 0.0006 | 0.0022 |
Cytoplasmic Foci | 0.005 | 0 | 0 | 0.0034 | 0.0001 | 0 | 0.0135 | 0.0001 | 0.0028 | 0 | 0.0025 | 0.0012 | 0.0012 | 0 | 0 | 0 | 0 | 0.0003 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0013 | 0 | 0 | 0.0001 | 0.0011 | 0 | 0.0008 | 0 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Endosome | 0.0044 | 0 | 0 | 0.0007 | 0.0013 | 0 | 0.016 | 0.0001 | 0.007 | 0.0014 | 0.0002 | 0.0029 | 0.0015 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 |
Golgi | 0.0015 | 0 | 0 | 0.0001 | 0 | 0 | 0.007 | 0 | 0.0027 | 0 | 0.0007 | 0.0019 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Lipid Particles | 0.0014 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0143 | 0 | 0.0007 | 0 | 0.0005 | 0.0014 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.0023 | 0.0001 | 0.0001 | 0.0004 | 0.0008 | 0 | 0.0024 | 0.0002 | 0.0004 | 0.0006 | 0.0008 | 0.0013 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 |
None | 0.0023 | 0.0086 | 0.0001 | 0.0008 | 0.0003 | 0 | 0.0023 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0028 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0184 | 0.001 | 0.0014 | 0.0017 | 0.0306 | 0.0002 | 0.0016 | 0.0051 | 0.0029 | 0.0017 | 0.0024 | 0.0029 | 0.0014 | 0.0053 | 0.0007 | 0.0003 | 0.0063 | 0.0008 |
Nucleolus | 0.0149 | 0.0162 | 0.0103 | 0.0076 | 0.0466 | 0.0399 | 0.0231 | 0.0155 | 0.0054 | 0.0178 | 0.0566 | 0.0312 | 0.0098 | 0.009 | 0.0061 | 0.003 | 0.0677 | 0.0294 |
Nucleus | 0.9018 | 0.9718 | 0.9868 | 0.9407 | 0.9089 | 0.9576 | 0.893 | 0.9646 | 0.972 | 0.9407 | 0.8904 | 0.9469 | 0.9626 | 0.9794 | 0.9828 | 0.9964 | 0.9046 | 0.96 |
Peroxisomes | 0.0009 | 0 | 0 | 0.0001 | 0 | 0 | 0.0048 | 0 | 0.0001 | 0 | 0.0157 | 0.0055 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0129 | 0.0014 | 0.0011 | 0.0425 | 0.0058 | 0.0011 | 0.004 | 0.0107 | 0.0047 | 0.0072 | 0.0028 | 0.0006 | 0.014 | 0.0053 | 0.01 | 0.0002 | 0.0187 | 0.0059 |
Vacuole | 0.001 | 0.0002 | 0 | 0.0004 | 0.0006 | 0.0001 | 0.0038 | 0.0007 | 0.0002 | 0.0205 | 0.0001 | 0.001 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0007 | 0 | 0 | 0.0003 | 0.0006 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0074 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.294 | 11.0486 | 9.3294 | 13.1715 | 12.7092 | 8.945 | 18.0088 | 21.7096 | 12.4858 | 17.4016 |
Translational Efficiency | 1.1389 | 1.7305 | 1.3525 | 0.6001 | 0.9677 | 1.1875 | 0.9685 | 0.6221 | 0.8333 | 0.7415 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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