Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 2 | 1 | 0 | 2 | 0 | – | 0 | 1 | 3 | 0 | 0 | 3 | 4 | 1 | 0 | 2 | 3 | 1 | 1 | 1 | 1 | 2 | 1 |
Bud | 9 | 6 | 5 | 19 | 16 | 41 | – | 66 | 34 | 59 | 32 | 34 | 6 | 10 | 9 | 1 | 4 | 1 | 2 | 1 | 0 | 1 | 4 | 4 |
Bud Neck | 4 | 0 | 2 | 1 | 3 | 7 | – | 16 | 5 | 9 | 2 | 5 | 0 | 2 | 3 | 0 | 1 | 2 | 2 | 2 | 1 | 1 | 5 | 4 |
Bud Site | 1 | 0 | 0 | 2 | 1 | 2 | – | 0 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 48 | 35 | 49 | 48 | 117 | 200 | – | 429 | 267 | 330 | 223 | 360 | 90 | 96 | 126 | 48 | 46 | 70 | 17 | 13 | 13 | 17 | 38 | 53 |
Endoplasmic Reticulum | 3 | 2 | 1 | 0 | 3 | 0 | – | 4 | 3 | 0 | 0 | 0 | 2 | 2 | 11 | 3 | 8 | 6 | 3 | 0 | 0 | 0 | 0 | 3 |
Endosome | 185 | 128 | 0 | 41 | 132 | 72 | – | 57 | 14 | 21 | 8 | 4 | 167 | 355 | 390 | 42 | 51 | 80 | 348 | 204 | 84 | 102 | 341 | 442 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 4 |
Mitochondria | 91 | 37 | 51 | 83 | 65 | 228 | – | 327 | 299 | 276 | 214 | 303 | 21 | 12 | 15 | 5 | 3 | 4 | 15 | 12 | 12 | 14 | 16 | 24 |
Nucleus | 2 | 1 | 0 | 0 | 0 | 0 | – | 5 | 2 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 4 | 6 |
Nuclear Periphery | 0 | 1 | 1 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 17 | 5 | 0 | 2 | 9 | 1 | – | 8 | 0 | 0 | 0 | 0 | 4 | 10 | 15 | 1 | 2 | 1 | 4 | 3 | 1 | 2 | 6 | 6 |
Peroxisomes | 9 | 2 | 0 | 22 | 18 | 35 | – | 20 | 2 | 11 | 3 | 4 | 4 | 0 | 42 | 0 | 5 | 0 | 2 | 2 | 0 | 1 | 0 | 1 |
SpindlePole | 125 | 38 | 25 | 67 | 133 | 98 | – | 163 | 66 | 69 | 36 | 52 | 44 | 140 | 174 | 20 | 21 | 32 | 46 | 23 | 15 | 15 | 51 | 44 |
Vac/Vac Membrane | 44 | 81 | 0 | 5 | 13 | 17 | – | 15 | 12 | 13 | 5 | 12 | 61 | 53 | 61 | 14 | 9 | 12 | 26 | 28 | 15 | 15 | 64 | 85 |
Unique Cell Count | 394 | 256 | 115 | 212 | 404 | 528 | 897 | 561 | 617 | 395 | 621 | 322 | 544 | 639 | 106 | 108 | 164 | 480 | 302 | 153 | 181 | 545 | 699 | |
Labelled Cell Count | 541 | 338 | 135 | 291 | 512 | 701 | 1111 | 706 | 794 | 524 | 781 | 403 | 686 | 853 | 135 | 152 | 214 | 480 | 302 | 153 | 181 | 545 | 699 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.2 | 3.4 | 3.6 | 4.0 | 3.4 | – | 3.5 | 3.1 | 3.5 | 3.4 | 3.1 | 5.1 | 5.2 | 5.0 | 6.7 | 7.0 | 6.5 | 5.4 | 5.4 | 5.7 |
Std Deviation (1e-4) | 6.5 | 0.8 | 0.7 | 1.0 | 1.1 | 1.3 | – | 1.1 | 2.8 | 4.1 | 3.8 | 0.5 | 0.9 | 0.8 | 0.8 | 1.2 | 4.5 | 1.6 | 2.8 | 2.5 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.26 | -0.0 | – | 0.03 | -0.14 | 0.06 | 0.0 | -0.14 | 0.59 | 0.63 | 0.56 | 0.98 | 1.06 | 0.94 | 0.67 | 0.68 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.8 | -2.8 | -0.9 | – | 1.1 | 1.0 | 2.1 | 2.6 | 3.0 | -2.9 | -5.9 | -5.4 | 0.4 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 9.8 | 12.8 | 12.1 | 7.5 | 8.4 | 8.9 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -9.4 | -14.0 | -14.7 | -6.8 | -7.2 | -8.7 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.0 | 0.9 | 1.2 | -0.5 | -0.4 | -0.5 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.0 | 3.5 | 3.5 | 4.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3288 | 1.4538 | 1.3465 | 1.7106 | 1.5734 | 1.3162 | 0.0645 | 0.3297 | 0.2956 | 0.2185 | 0.0131 | 0.1563 | 2.2639 | 2.2698 | 2.396 | 2.2172 | 2.324 | 1.8245 |
Actin | 0.007 | 0.0001 | 0.0069 | 0.0074 | 0.0276 | 0.0028 | 0.0145 | 0.0001 | 0.0048 | 0.004 | 0.0011 | 0.0017 | 0.0534 | 0.0001 | 0.0129 | 0.0105 | 0.0004 | 0.0001 |
Bud | 0.0002 | 0.0002 | 0.0041 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0029 | 0.0013 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0001 |
Bud Neck | 0.0015 | 0.0008 | 0.0022 | 0.0053 | 0.0013 | 0.0005 | 0.0006 | 0.0008 | 0.0009 | 0.0051 | 0.0002 | 0.001 | 0.0037 | 0.0016 | 0.0003 | 0.001 | 0.0009 | 0.0007 |
Bud Periphery | 0.0002 | 0.0003 | 0.0011 | 0.0002 | 0.001 | 0.0001 | 0.0007 | 0.0001 | 0.0037 | 0.0019 | 0.0004 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0008 | 0.0001 | 0.0001 |
Bud Site | 0.0013 | 0.0017 | 0.0015 | 0.0009 | 0.0013 | 0.0002 | 0.0031 | 0.002 | 0.0091 | 0.0237 | 0.0016 | 0.0016 | 0.0104 | 0.003 | 0.0015 | 0.0022 | 0.0019 | 0.0004 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0012 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.04 | 0.0554 | 0.0675 | 0.0059 | 0.0064 | 0.0392 | 0.0855 | 0.0925 | 0.097 | 0.1019 | 0.0247 | 0.0319 | 0.071 | 0.1185 | 0.1333 | 0.1001 | 0.1765 | 0.122 |
Cytoplasmic Foci | 0.234 | 0.3282 | 0.2468 | 0.3215 | 0.3324 | 0.3295 | 0.2197 | 0.2799 | 0.2125 | 0.1966 | 0.1848 | 0.28 | 0.2505 | 0.2414 | 0.2435 | 0.3261 | 0.4321 | 0.3087 |
Eisosomes | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0061 | 0.0013 | 0.0021 | 0.0012 | 0.0053 | 0.0012 | 0.0057 | 0.0024 | 0.0067 | 0.0013 | 0.0044 | 0.0017 | 0.0067 | 0.0044 | 0.0043 | 0.0015 | 0.0002 | 0.0021 |
Endosome | 0.4119 | 0.3848 | 0.391 | 0.398 | 0.2778 | 0.3663 | 0.3548 | 0.3739 | 0.3871 | 0.4236 | 0.405 | 0.4494 | 0.3079 | 0.2852 | 0.2849 | 0.2707 | 0.2719 | 0.3469 |
Golgi | 0.0214 | 0.0204 | 0.0365 | 0.0364 | 0.0384 | 0.0215 | 0.0313 | 0.0166 | 0.0291 | 0.0331 | 0.0307 | 0.0354 | 0.0164 | 0.0108 | 0.0326 | 0.0326 | 0.0398 | 0.0212 |
Lipid Particles | 0.1321 | 0.0999 | 0.0934 | 0.1129 | 0.0942 | 0.1235 | 0.1303 | 0.1023 | 0.073 | 0.0658 | 0.1219 | 0.1235 | 0.0858 | 0.076 | 0.0576 | 0.0742 | 0.0413 | 0.0848 |
Mitochondria | 0.0102 | 0.0043 | 0.0027 | 0.003 | 0.0192 | 0.0012 | 0.0206 | 0.0008 | 0.0211 | 0.0454 | 0.0231 | 0.0059 | 0.0089 | 0.0047 | 0.0068 | 0.058 | 0.0015 | 0.0016 |
None | 0.0278 | 0.0173 | 0.0571 | 0.0039 | 0.029 | 0.0225 | 0.0339 | 0.0685 | 0.0845 | 0.0572 | 0.1276 | 0.022 | 0.0904 | 0.1651 | 0.1553 | 0.0053 | 0.0004 | 0.0757 |
Nuclear Periphery | 0.0129 | 0.0013 | 0.0069 | 0.0021 | 0.0317 | 0.0043 | 0.018 | 0.0015 | 0.0044 | 0.0007 | 0.0038 | 0.0031 | 0.0139 | 0.0027 | 0.004 | 0.0022 | 0.0001 | 0.0009 |
Nucleolus | 0.0021 | 0.0059 | 0.0011 | 0.0007 | 0.0018 | 0.0005 | 0.0041 | 0.0015 | 0.0008 | 0.0007 | 0.0005 | 0.0019 | 0.0031 | 0.0055 | 0.0006 | 0.0035 | 0.0005 | 0.0005 |
Nucleus | 0.0129 | 0.0021 | 0.0047 | 0.0009 | 0.0047 | 0.0008 | 0.0063 | 0.0026 | 0.002 | 0.0007 | 0.0009 | 0.0013 | 0.0164 | 0.0063 | 0.0043 | 0.0233 | 0.0002 | 0.0013 |
Peroxisomes | 0.0485 | 0.0483 | 0.0548 | 0.0879 | 0.1064 | 0.0647 | 0.0152 | 0.0235 | 0.0241 | 0.0213 | 0.0375 | 0.0134 | 0.0216 | 0.0187 | 0.0437 | 0.0556 | 0.0275 | 0.0169 |
Punctate Nuclear | 0.0147 | 0.0105 | 0.004 | 0.0073 | 0.0112 | 0.0121 | 0.0168 | 0.0047 | 0.0025 | 0.0007 | 0.0065 | 0.012 | 0.0151 | 0.0046 | 0.0042 | 0.0038 | 0.0022 | 0.0039 |
Vacuole | 0.0127 | 0.0154 | 0.0129 | 0.0029 | 0.0049 | 0.0078 | 0.0301 | 0.0239 | 0.027 | 0.0103 | 0.0197 | 0.0105 | 0.0188 | 0.0471 | 0.0082 | 0.0232 | 0.0017 | 0.0105 |
Vacuole Periphery | 0.0024 | 0.0017 | 0.0021 | 0.0011 | 0.0044 | 0.0013 | 0.0077 | 0.002 | 0.0064 | 0.0034 | 0.005 | 0.0032 | 0.0041 | 0.0031 | 0.0014 | 0.0044 | 0.0007 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.1418 | 9.5765 | 7.1062 | 8.9828 | 2.3149 | 10.7067 | 8.8759 | 6.5352 | 12.5082 | 13.4791 |
Translational Efficiency | 1.635 | 1.0961 | 1.8217 | 1.3226 | 6.8262 | 2.0781 | 1.3351 | 1.6037 | 1.5258 | 1.5603 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1038 | 1340 | 746 | 983 | 1323 | 1465 | 1326 | 826 | 2361 | 2805 | 2072 | 1809 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 632.41 | 758.54 | 918.48 | 837.54 | 671.11 | 762.11 | 873.79 | 797.73 | 654.10 | 760.40 | 889.88 | 819.36 |
Standard Deviation | 71.24 | 107.35 | 105.82 | 114.88 | 93.48 | 100.29 | 100.60 | 108.66 | 86.59 | 103.74 | 104.73 | 113.83 |
Intensity Change Log 2 | — | 0.262365 | 0.538388 | 0.405298 | — | 0.183450 | 0.380737 | 0.249351 | — | 0.222275 | 0.459375 | 0.327116 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000109 | 0.000131 | 0.000299 | 0.000990 | 0.000137 | 0.000074 | 0.000222 | 0.000199 | 0.000125 | 0.000101 | 0.000250 | 0.000629 |
Bud Neck | 0.055004 | 0.008663 | 0.026906 | 0.019487 | 0.024426 | 0.012344 | 0.015905 | 0.039007 | 0.037870 | 0.010585 | 0.019866 | 0.028400 |
Bud Site | 0.004602 | 0.005555 | 0.010816 | 0.020377 | 0.003986 | 0.004552 | 0.010416 | 0.012304 | 0.004257 | 0.005031 | 0.010560 | 0.016691 |
Cell Periphery | 0.000225 | 0.000024 | 0.000047 | 0.000066 | 0.000057 | 0.000065 | 0.000022 | 0.000199 | 0.000131 | 0.000046 | 0.000031 | 0.000127 |
Cytoplasm | 0.083976 | 0.021568 | 0.012869 | 0.088724 | 0.042808 | 0.018431 | 0.014111 | 0.153740* | 0.060907 | 0.019930 | 0.013664 | 0.118410* |
Cytoplasmic Foci | 0.248422* | 0.171319 | 0.109335 | 0.183239* | 0.214393* | 0.145698 | 0.131109 | 0.144127 | 0.229354* | 0.157938 | 0.123270 | 0.165380 |
Eisosomes | 0.000279 | 0.000029 | 0.000060 | 0.000055 | 0.000099 | 0.000059 | 0.000024 | 0.000191 | 0.000178 | 0.000045 | 0.000037 | 0.000117 |
Endoplasmic Reticulum | 0.000838 | 0.000384 | 0.001129 | 0.002053 | 0.000256 | 0.000421 | 0.000492 | 0.000950 | 0.000512 | 0.000404 | 0.000721 | 0.001549 |
Endosome | 0.355313* | 0.622060* | 0.524316* | 0.317248* | 0.517631* | 0.631759* | 0.605403* | 0.250417* | 0.446269* | 0.627126* | 0.576208* | 0.286733* |
Golgi | 0.026392 | 0.029505 | 0.007330 | 0.005073 | 0.017704 | 0.031620 | 0.006625 | 0.004415 | 0.021524 | 0.030610 | 0.006879 | 0.004773 |
Lipid Particles | 0.066939 | 0.013257 | 0.009957 | 0.010152 | 0.053399 | 0.042180 | 0.006322 | 0.017686 | 0.059352 | 0.028363 | 0.007631 | 0.013592 |
Mitochondria | 0.019514 | 0.010403 | 0.002326 | 0.001486 | 0.010210 | 0.015316 | 0.001943 | 0.007540 | 0.014300 | 0.012969 | 0.002081 | 0.004250 |
Mitotic Spindle | 0.000380 | 0.021229 | 0.043823 | 0.050965 | 0.003112 | 0.007844 | 0.052148 | 0.023152 | 0.001911 | 0.014238 | 0.049151 | 0.038265 |
None | 0.001945 | 0.000231 | 0.000242 | 0.001029 | 0.000871 | 0.000225 | 0.000145 | 0.004794 | 0.001343 | 0.000228 | 0.000180 | 0.002748 |
Nuclear Periphery | 0.000860 | 0.000302 | 0.001255 | 0.001389 | 0.000390 | 0.000327 | 0.000807 | 0.001727 | 0.000597 | 0.000315 | 0.000969 | 0.001543 |
Nuclear Periphery Foci | 0.000424 | 0.001781 | 0.014134 | 0.010379 | 0.001399 | 0.003032 | 0.012139 | 0.003223 | 0.000970 | 0.002434 | 0.012857 | 0.007111 |
Nucleolus | 0.002583 | 0.001411 | 0.017850 | 0.003404 | 0.003149 | 0.002434 | 0.006556 | 0.011144 | 0.002900 | 0.001945 | 0.010622 | 0.006938 |
Nucleus | 0.031397 | 0.003543 | 0.034542 | 0.024452 | 0.012611 | 0.004156 | 0.004814 | 0.128987 | 0.020870 | 0.003863 | 0.015517 | 0.072183 |
Peroxisomes | 0.046161 | 0.035004 | 0.070049 | 0.065670 | 0.052694 | 0.036010 | 0.050748 | 0.032991 | 0.049822 | 0.035530 | 0.057697 | 0.050749 |
Vacuole | 0.051095 | 0.048605 | 0.109231 | 0.192124 | 0.038699 | 0.038879 | 0.078540 | 0.160976 | 0.044149 | 0.043525 | 0.089590 | 0.177902 |
Vacuole Periphery | 0.003542 | 0.004996 | 0.003484 | 0.001638 | 0.001968 | 0.004574 | 0.001509 | 0.002233 | 0.002660 | 0.004776 | 0.002220 | 0.001909 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.19 | -4.56 | -7.57 | -7.27 | -4.86 | 1.86 | -2.24 | -2.60 | -4.45 | -0.17 | 0.88 | -4.27 | -7.83 | -8.33 | -4.71 |
Bud Neck | 10.74 | 5.43 | 7.28 | -7.08 | 1.96 | 5.03 | 2.53 | -2.49 | -5.92 | -4.32 | 11.67 | 6.36 | 3.56 | -8.55 | -2.63 |
Bud Site | -0.81 | -4.23 | -7.99 | -8.54 | -4.53 | -0.57 | -7.15 | -4.13 | -3.57 | -0.15 | -1.04 | -7.83 | -9.07 | -8.45 | -3.35 |
Cell Periphery | 9.97 | 8.58 | 7.76 | -8.86 | -2.80 | -0.77 | 7.09 | -6.70 | -5.62 | -8.79 | 8.19 | 10.23 | 1.47 | -7.33 | -9.76 |
Cytoplasm | 11.13 | 12.38 | -4.18 | -13.93 | -14.95 | 6.83 | 5.84 | -13.55 | -17.49 | -16.97 | 12.69 | 12.79 | -12.96 | -22.23 | -22.27 |
Cytoplasmic Foci | 9.85 | 18.54 | 11.77 | 2.61 | -7.33 | 10.34 | 13.40 | 8.35 | -0.69 | -3.14 | 14.04 | 21.98 | 14.01 | 1.17 | -6.75 |
Eisosomes | 13.69 | 11.41 | 11.82 | -9.31 | 0.98 | 5.43 | 11.51 | -5.81 | -9.51 | -12.90 | 14.54 | 15.09 | 6.35 | -11.24 | -12.19 |
Endoplasmic Reticulum | 2.24 | -2.98 | -4.75 | -7.54 | -2.17 | -2.41 | -6.61 | -4.62 | -3.46 | -1.79 | 1.05 | -4.68 | -6.95 | -8.19 | -3.90 |
Endosome | -20.28 | -9.26 | 10.61 | 33.69* | 19.50 | -9.66 | -3.23 | 21.70 | 32.10 | 25.37* | -20.03 | -9.10 | 23.82 | 46.78 | 33.20* |
Golgi | -1.07 | 9.70 | 11.62 | 14.09 | 5.06 | -6.35 | 9.06 | 10.43 | 13.95 | 2.72 | -5.34 | 13.21 | 15.77 | 19.85 | 5.04 |
Lipid Particles | 12.77 | 13.46 | 14.28 | 2.34 | 0.44 | 2.40 | 13.54 | 9.74 | 7.49 | -4.75 | 9.90 | 19.29 | 17.15 | 8.15 | -3.95 |
Mitochondria | 4.19 | 9.03 | 9.97 | 7.36 | 1.74 | -2.47 | 5.75 | 1.99 | 4.15 | -2.32 | 0.86 | 10.55 | 8.04 | 7.57 | -1.54 |
Mitotic Spindle | -8.89 | -8.94 | -11.38 | -6.39 | -1.41 | -3.37 | -13.35 | -6.57 | -5.27 | 5.40 | -9.12 | -16.15 | -12.92 | -8.54 | 2.56 |
None | 1.89 | 1.95 | 0.95 | -2.39 | -2.73 | 2.86 | 2.68 | -2.51 | -3.27 | -3.23 | 2.85 | 2.88 | -1.50 | -3.84 | -3.86 |
Nuclear Periphery | 1.99 | -2.63 | -4.76 | -10.45 | -2.22 | 0.70 | -5.87 | -3.75 | -3.91 | -1.21 | 2.12 | -5.23 | -6.54 | -8.73 | -2.69 |
Nuclear Periphery Foci | -5.26 | -8.21 | -7.91 | -6.86 | 1.60 | -1.86 | -7.78 | -4.04 | -0.59 | 6.27 | -3.21 | -11.15 | -8.50 | -6.15 | 4.27 |
Nucleolus | 2.23 | -7.13 | -3.20 | -4.55 | 5.83 | 1.21 | -4.91 | -5.27 | -5.98 | -1.30 | 2.16 | -8.70 | -5.94 | -7.32 | 3.69 |
Nucleus | 10.13 | -1.42 | -1.65 | -10.56 | 0.13 | 5.40 | 2.69 | -14.34 | -15.87 | -15.19 | 11.26 | 0.84 | -13.05 | -18.45 | -13.02 |
Peroxisomes | 3.18 | -4.18 | -0.55 | -3.33 | 3.46 | 4.90 | 1.28 | 5.78 | 1.39 | 4.50 | 5.83 | -1.75 | 3.14 | -1.91 | 4.49 |
Vacuole | 0.04 | -9.23 | -24.56 | -24.79 | -14.16 | 0.08 | -11.57 | -18.39 | -18.35 | -10.29 | -0.07 | -14.51 | -30.70 | -30.68 | -18.52 |
Vacuole Periphery | -2.31 | 0.25 | 6.95 | 6.30 | 4.87 | -6.24 | 2.16 | 0.42 | 5.51 | -0.84 | -5.75 | 2.05 | 4.75 | 8.60 | 2.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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