Standard name
Human Ortholog
Description Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under the nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Bud – 0.06 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Bud Neck – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 – 0 – – 0 – – – – 0 0 0 – – – – – – – – –
Cell Periphery – 0.85 0.87 – 0.87 – – 0.75 – – – – 0.92 0.92 0.86 – – – 0.95 0.7 0.85 0.72 0.73 0.72
Cytoplasm – 0.06 0.11 – 0 – – 0.2 – – – – 0.07 0.07 0.12 – – – 0 0.11 0 0 0 0
Endoplasmic Reticulum – 0.09 0 – 0 – – 0.05 – – – – 0 0 0 – – – 0 0 0 0 0 0
Endosome – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0.12 0.08 0.22 0.2 0.2
Nucleus – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 – 0 – – 0 – – – – 1 0 2 – – – 0 2 1 1 4 2
Bud – 2 1 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 2 1 3
Bud Neck – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 – 0 – – 0 – – – – 0 0 0 – – – – – – – – –
Cell Periphery – 28 103 – 39 – – 15 – – – – 119 119 64 – – – 73 92 160 131 143 155
Cytoplasm – 2 13 – 2 – – 4 – – – – 9 9 9 – – – 0 14 3 1 1 2
Endoplasmic Reticulum – 3 5 – 2 – – 1 – – – – 2 2 0 – – – 0 3 4 3 3 3
Endosome – 0 0 – 1 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 1 0 0
Mitochondria – 0 0 – 1 – – 0 – – – – 0 0 0 – – – 2 15 15 39 39 42
Nucleus – 0 0 – 0 – – 0 – – – – 0 0 1 – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 – 0 – – 0 – – – – 0 0 1 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 – 0 – – 0 – – – – 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 – 0 – – 0 – – – – 0 0 1 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 1 – 0 – – 0 – – – – 1 1 1 – – – 0 0 0 0 0 1
Unique Cell Count 33 119 45 20 129 129 74 77 132 189 184 197 215
Labelled Cell Count 35 123 45 20 132 131 79 77 132 189 184 197 215


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 178.4 156.1 – 192.0 – – 251.4 – – – – 127.4 127.4 117.8 – – – 153.3 162.1 181.5
Std Deviation (1e-4) – 40.3 49.1 – 57.7 – – 91.9 – – – – 41.8 41.8 46.7 – – – 46.9 52.1 58.0
Intensity Change (Log2) – – – – 0.3 – – 0.69 – – – – -0.29 -0.29 -0.41 – – – -0.03 0.05 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – 0 – – 0 – – – – 0 0 0 – – –
Bud – 0 – – 0 – – – – 0 0 0 – – –
Bud Neck – 0 – – 0 – – – – 0 0 0 – – –
Bud Site – 0 – – 0 – – – – 0 0 0 – – –
Cell Periphery – 0 – – -1.3 – – – – 1.5 1.5 0 – – –
Cytoplasm – 0 – – 0 – – – – -1.1 -1.1 0.3 – – –
Endoplasmic Reticulum – 0 – – 0 – – – – 0 0 0 – – –
Endosome – 0 – – 0 – – – – 0 0 0 – – –
Golgi – 0 – – 0 – – – – 0 0 0 – – –
Mitochondria – 0 – – 0 – – – – 0 0 0 – – –
Nucleus – 0 – – 0 – – – – 0 0 0 – – –
Nuclear Periphery – 0 – – 0 – – – – 0 0 0 – – –
Nucleolus – 0 – – 0 – – – – 0 0 0 – – –
Peroxisomes – 0 – – 0 – – – – 0 0 0 – – –
SpindlePole – 0 – – 0 – – – – 0 0 0 – – –
Vacuole – 0 – – 0 – – – – 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1104 9.6931 7.9798 4.8364 5.9289 7.3734 7.2634 17.6067 11.6501 7.3365 9.3119 13.6944 6.4505 20.5174 9.3219 7.259 10.6626 14.5807
Actin 0.0515 0.0188 0.0611 0.0193 0.0344 0.0364 0.0336 0.0131 0.0215 0.0122 0.0319 0.0119 0.0258 0.0204 0.0231 0.017 0.0293 0.01
Bud 0.0257 0.0189 0.0082 0.012 0.0159 0.0199 0.0206 0.0196 0.0176 0.0264 0.0142 0.0136 0.0259 0.0121 0.0272 0.0286 0.0316 0.0122
Bud Neck 0.0086 0.0036 0.0067 0.0265 0.0341 0.0212 0.0085 0.0027 0.0157 0.0071 0.0228 0.0411 0.0075 0.0031 0.0081 0.0089 0.0049 0.0377
Bud Periphery 0.1188 0.0377 0.0564 0.1152 0.2231 0.1144 0.1099 0.0434 0.0716 0.18 0.109 0.1162 0.1186 0.0235 0.0864 0.1003 0.119 0.1008
Bud Site 0.0798 0.2792 0.0908 0.0769 0.0176 0.0101 0.1231 0.2995 0.1603 0.0776 0.0795 0.0165 0.1875 0.2958 0.1121 0.0346 0.0515 0.0077
Cell Periphery 0.2226 0.3383 0.3728 0.3504 0.3704 0.3974 0.4417 0.4683 0.4717 0.3701 0.4826 0.5608 0.3293 0.4865 0.4321 0.3997 0.4805 0.6017
Cytoplasm 0.1072 0.1473 0.1328 0.0969 0.106 0.197 0.0863 0.0678 0.1069 0.1941 0.1543 0.134 0.0657 0.0652 0.1178 0.2978 0.0622 0.0858
Cytoplasmic Foci 0.0214 0.018 0.0167 0.0205 0.0106 0.0153 0.012 0.0044 0.0107 0.0073 0.0258 0.0101 0.0085 0.0027 0.0108 0.0021 0.0157 0.0156
Eisosomes 0.0067 0.0072 0.015 0.0029 0.0108 0.006 0.0066 0.0071 0.0114 0.0032 0.0056 0.0035 0.0094 0.0162 0.0103 0.0012 0.0033 0.0045
Endoplasmic Reticulum 0.0217 0.0106 0.0162 0.0093 0.0122 0.011 0.0113 0.0058 0.0076 0.0137 0.007 0.0132 0.0197 0.0025 0.0109 0.0087 0.0081 0.0101
Endosome 0.031 0.0097 0.0163 0.0472 0.0587 0.0134 0.0107 0.0018 0.0079 0.0003 0.0113 0.0031 0.0104 0.0003 0.0117 0.0008 0.0064 0.0065
Golgi 0.0077 0.0024 0.0032 0.0244 0.0127 0.0057 0.0022 0.0009 0.0008 0.0005 0.0011 0.0005 0.0026 0.0003 0.001 0.001 0.0034 0.0025
Lipid Particles 0.0131 0.0042 0.0055 0.0146 0.027 0.0043 0.0064 0.0034 0.0026 0.0019 0.0039 0.0028 0.006 0.0016 0.0023 0.0021 0.0326 0.0031
Mitochondria 0.0175 0.0032 0.0163 0.0386 0.0088 0.0041 0.0066 0.0013 0.0029 0.005 0.0013 0.0012 0.0067 0.0017 0.0039 0.001 0.0321 0.0087
None 0.1693 0.0555 0.1183 0.0829 0.0022 0.1038 0.0789 0.0288 0.0547 0.0595 0.0161 0.0419 0.1305 0.0476 0.1026 0.017 0.0634 0.0329
Nuclear Periphery 0.0174 0.0015 0.002 0.0018 0.0025 0.0015 0.003 0.0021 0.0011 0.0012 0.0011 0.0011 0.0017 0.0006 0.0017 0.002 0.0033 0.001
Nucleolus 0.0028 0.0026 0.0048 0.0019 0.0026 0.0018 0.0022 0.0043 0.0031 0.003 0.001 0.001 0.0025 0.0008 0.0026 0.0046 0.0042 0.0036
Nucleus 0.0107 0.0016 0.0025 0.0016 0.002 0.0023 0.0047 0.0025 0.0016 0.0016 0.0012 0.0023 0.0018 0.0008 0.0018 0.0053 0.0057 0.0013
Peroxisomes 0.0031 0.0006 0.0025 0.0105 0.0033 0.0004 0.0007 0.0002 0.0025 0.0006 0.0009 0.0003 0.001 0.0002 0.0003 0.0002 0.0022 0.0012
Punctate Nuclear 0.0019 0.0007 0.002 0.0005 0.0007 0.0005 0.0022 0.0036 0.0008 0.0006 0.0003 0.0028 0.0007 0.0001 0.0004 0.001 0.0082 0.0008
Vacuole 0.0542 0.0373 0.0467 0.0424 0.0401 0.0304 0.0276 0.0191 0.026 0.0313 0.0271 0.0211 0.0364 0.0177 0.0309 0.063 0.0298 0.0507
Vacuole Periphery 0.0073 0.0009 0.0032 0.0036 0.0042 0.003 0.0013 0.0004 0.0011 0.0028 0.0019 0.0011 0.0017 0.0002 0.002 0.0031 0.0025 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.1715 11.1789 10.735 13.8453 14.783 5.1464 7.6462 8.7506 8.4328 8.1736
Translational Efficiency 1.6227 1.8451 1.0437 0.9041 0.8031 0.4849 0.4412 0.4133 0.4345 0.2873

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
99 1120 247 1748 1306 1585 107 1562 1405 2705 354 3310

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1932.52 7973.65 1620.48 834.19 11828.54 6869.03 2528.70 777.62 11131.24 7326.40 1895.00 807.49
Standard Deviation 1007.86 3485.64 743.92 265.02 3373.55 2932.19 1469.32 106.13 4130.95 3219.38 1101.21 207.86
Intensity Change Log 2 — 2.044757 -0.254062 -1.212035 — -0.784094 -2.225804 -3.927063 — 0.109160 -1.729693 -3.093838

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.016514 0.000619 0.029657 0.010677 0.000328 0.000875 0.021153 0.002343 0.001469 0.000769 0.027087 0.006744
Bud Neck 0.030621 0.001057 0.022293 0.018603 0.000254 0.001719 0.032755 0.014817 0.002394 0.001445 0.025455 0.016816
Bud Site 0.126517* 0.009249 0.203749* 0.099106 0.001467 0.018426 0.132465* 0.064456 0.010278 0.014626 0.182202* 0.082755
Cell Periphery 0.579718* 0.942153* 0.561682* 0.013000 0.972361* 0.920292* 0.720934* 0.003061 0.944695* 0.929343* 0.609818* 0.008310
Cytoplasm 0.060035 0.003086 0.031248 0.421046* 0.000038 0.019002 0.003940 0.474640* 0.004266 0.012412 0.022994 0.446338*
Cytoplasmic Foci 0.021203 0.000770 0.008453 0.023043 0.000128 0.000852 0.001450 0.030353 0.001613 0.000818 0.006336 0.026492
Eisosomes 0.014295 0.005687 0.023449 0.001280 0.008566 0.008015 0.026901 0.000443 0.008970 0.007051 0.024493 0.000885
Endoplasmic Reticulum 0.003462 0.000319 0.017541 0.002640 0.000488 0.001307 0.016569 0.001641 0.000697 0.000898 0.017247 0.002169
Endosome 0.005441 0.000142 0.002431 0.004004 0.000093 0.000442 0.001489 0.005414 0.000469 0.000317 0.002147 0.004669
Golgi 0.010609 0.000970 0.000984 0.001877 0.000537 0.001640 0.001038 0.002364 0.001247 0.001363 0.001000 0.002107
Lipid Particles 0.010652 0.000286 0.002213 0.002484 0.000135 0.002482 0.002315 0.002366 0.000876 0.001573 0.002244 0.002428
Mitochondria 0.002203 0.002745 0.008581 0.001923 0.000531 0.001198 0.001044 0.003034 0.000649 0.001839 0.006303 0.002447
Mitotic Spindle 0.001799 0.000331 0.001320 0.022027 0.000409 0.000631 0.000854 0.010868 0.000507 0.000507 0.001179 0.016761
None 0.000081 0.000261 0.000486 0.003590 0.000035 0.000592 0.000075 0.003828 0.000039 0.000455 0.000362 0.003702
Nuclear Periphery 0.000766 0.000442 0.000467 0.001426 0.000583 0.000823 0.000469 0.001023 0.000596 0.000665 0.000468 0.001236
Nuclear Periphery Foci 0.000623 0.000218 0.001438 0.000878 0.000371 0.000382 0.000548 0.000570 0.000389 0.000314 0.001169 0.000733
Nucleolus 0.000042 0.000210 0.000321 0.000342 0.000163 0.000217 0.000145 0.000356 0.000154 0.000214 0.000268 0.000348
Nucleus 0.021598 0.001058 0.053361 0.221324* 0.000202 0.001292 0.007592 0.275576* 0.001709 0.001195 0.039527 0.246926*
Peroxisomes 0.001136 0.000009 0.000663 0.001372 0.000002 0.000043 0.000182 0.001157 0.000082 0.000029 0.000518 0.001271
Vacuole 0.091499 0.024343 0.029030 0.147581 0.008780 0.015668 0.027157 0.099637 0.014609 0.019260 0.028464 0.124956
Vacuole Periphery 0.001188 0.006046 0.000632 0.001777 0.004528 0.004101 0.000926 0.002052 0.004293 0.004906 0.000721 0.001907

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.08 -1.67 2.23 -4.72 4.51 -2.29 -2.41 -2.79 -1.42 2.27 1.48 -5.54 -2.70 -4.83 5.14
Bud Neck 2.50 0.73 1.54 -11.39 4.08 -5.61 -5.33 -9.84 -8.22 3.46 1.02 -9.01 -8.38 -13.62 5.35
Bud Site 4.89 -2.37 3.13 -10.44 7.93 -8.13 -5.24 -10.69 -5.24 3.64 -1.97 -11.22 -10.99 -10.59 8.77
Cell Periphery -9.18 0.81 13.76 257.71* 20.30 10.85 7.89 414.33* 205.14* 21.29* 2.88 16.83 206.61 303.58* 27.54*
Cytoplasm 4.16 1.49 -18.56 -54.40* -35.00 -8.07 -2.58 -56.97* -51.54 -54.12* -4.44 -5.33 -76.18* -73.29* -53.02*
Cytoplasmic Foci 3.31 1.65 0.53 -16.96 -3.11 -4.49 -2.09 -18.73 -18.02 -14.67 1.59 -3.75 -21.41 -24.91 -7.53
Eisosomes 1.42 -1.26 2.30 8.32 7.97 0.47 -4.21 8.91 10.12 6.30 1.76 -5.90 8.91 12.82 10.15
Endoplasmic Reticulum 3.65 -3.46 0.41 -17.13 3.62 -1.48 -1.99 -4.39 -1.84 1.79 -0.47 -4.45 -5.55 -6.46 3.96
Endosome 2.36 0.97 1.14 -9.86 0.54 -2.92 -1.67 -6.81 -6.03 -2.10 0.83 -4.38 -8.38 -9.84 -1.04
Golgi 1.68 1.68 1.71 0.36 0.39 -4.65 -1.43 -1.88 0.63 -0.61 -0.31 0.50 0.31 0.94 -0.20
Lipid Particles 1.60 1.23 1.32 -4.58 0.88 -4.19 -1.31 -3.98 0.65 0.23 -1.23 -2.13 -2.12 -1.07 0.77
Mitochondria -0.33 -2.23 0.58 1.68 3.00 -2.77 -1.58 -3.38 -1.66 -1.74 -2.98 -3.32 -3.49 0.56 2.73
Mitotic Spindle 1.64 0.00 -6.63 -8.11 -7.03 -3.05 -1.52 -5.79 -5.63 -5.09 0.00 -2.55 -9.82 -9.83 -8.48
None -1.65 -3.65 -9.96 -9.15 -7.74 -1.75 -1.48 -9.52 -5.75 -9.28 -2.17 -4.19 -13.91 -9.49 -11.19
Nuclear Periphery 2.23 1.03 -8.44 -15.38 -12.16 -1.47 0.64 -6.13 -9.18 -10.94 -0.46 0.21 -8.92 -16.93 -15.40
Nuclear Periphery Foci 2.41 -2.68 -2.10 -7.32 2.06 -0.04 -0.92 -1.26 -1.86 -0.19 0.29 -2.94 -2.02 -5.11 2.14
Nucleolus -3.44 -3.64 -6.31 -2.76 0.23 -1.06 -0.07 -3.08 -1.93 -2.58 -1.64 -2.64 -5.26 -3.19 -0.58
Nucleus 2.39 -2.63 -19.07 -45.16 -14.45 -5.70 -1.78 -46.06 -45.87 -33.83* 1.04 -5.71 -63.03 -64.17 -23.72
Peroxisomes 2.04 0.61 0.56 -4.75 -0.16 -7.96 -1.99 -6.35 -6.03 -3.16 1.34 -2.82 -6.39 -7.29 -0.99
Vacuole 3.95 3.02 -7.95 -33.28 -23.71 -5.46 -2.87 -28.54 -27.15 -13.80 -2.57 -4.31 -42.10 -42.85 -26.62
Vacuole Periphery -7.58 1.16 0.67 9.23 -0.47 0.55 4.84 4.08 6.28 -0.72 -0.86 5.23 4.59 11.15 -1.04
Standard name
Human Ortholog
Description Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under the nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p
Localization
Cell Percentages cell periphery (21%), mitochondrion (7%), mixed (36%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mep2

Mep2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mep2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available