Standard name | |
---|---|
Human Ortholog | |
Description | Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 2 | 1 | 6 | 3 | 5 | 4 | 2 | 5 | 1 | 1 | 3 | 2 | 2 | 0 | 0 | 0 | 1 | 5 | 3 | 2 | 3 | 7 |
Bud | 0 | 0 | 2 | 2 | 4 | 4 | 13 | 14 | 11 | 10 | 2 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 19 | 4 | 18 | 35 | 38 |
Bud Neck | 0 | 0 | 1 | 1 | 3 | 2 | 5 | 2 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 8 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 7 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 3 | 0 | 2 |
Cytoplasm | 60 | 147 | 123 | 128 | 163 | 199 | 166 | 230 | 184 | 183 | 32 | 75 | 470 | 403 | 551 | 207 | 157 | 133 | 24 | 81 | 85 | 255 | 246 | 234 |
Endoplasmic Reticulum | 6 | 26 | 8 | 11 | 7 | 7 | 5 | 4 | 12 | 11 | 4 | 7 | 27 | 8 | 5 | 4 | 13 | 7 | 20 | 32 | 7 | 5 | 7 | 9 |
Endosome | 3 | 3 | 1 | 3 | 14 | 11 | 17 | 9 | 2 | 4 | 1 | 1 | 3 | 10 | 5 | 2 | 3 | 1 | 6 | 35 | 18 | 20 | 18 | 32 |
Golgi | 7 | 22 | 2 | 8 | 9 | 4 | 4 | 2 | 1 | 1 | 1 | 0 | 5 | 12 | 9 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 5 | 3 |
Mitochondria | 0 | 1 | 5 | 7 | 8 | 48 | 63 | 79 | 97 | 81 | 19 | 35 | 0 | 2 | 1 | 7 | 1 | 4 | 0 | 1 | 7 | 14 | 13 | 18 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 9 | 3 | 4 | 0 | 0 | 0 | 1 | 3 | 2 | 3 | 2 | 4 | 24 | 1 | 2 | 2 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 53 | 113 | 22 | 14 | 12 | 14 |
Nucleolus | 4 | 15 | 0 | 1 | 10 | 6 | 16 | 17 | 5 | 8 | 1 | 1 | 2 | 7 | 3 | 0 | 0 | 0 | 0 | 7 | 0 | 0 | 1 | 1 |
Peroxisomes | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 2 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 4 | 1 | 1 | 3 | 2 | 4 | 1 | 1 | 1 | 4 | 27 | 4 | 12 | 13 | 17 |
Vac/Vac Membrane | 37 | 155 | 18 | 15 | 25 | 10 | 35 | 57 | 14 | 15 | 5 | 4 | 46 | 42 | 49 | 4 | 12 | 3 | 11 | 90 | 8 | 26 | 29 | 28 |
Unique Cell Count | 108 | 344 | 155 | 165 | 229 | 253 | 277 | 363 | 284 | 274 | 60 | 103 | 549 | 477 | 626 | 214 | 171 | 141 | 134 | 453 | 172 | 389 | 402 | 437 |
Labelled Cell Count | 118 | 378 | 163 | 178 | 252 | 298 | 340 | 437 | 344 | 338 | 68 | 126 | 561 | 494 | 643 | 232 | 191 | 157 | 134 | 453 | 172 | 389 | 402 | 437 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.5 | 29.0 | 8.7 | 9.1 | 8.5 | 6.9 | 6.7 | 7.2 | 6.0 | 6.3 | 7.5 | 5.6 | 10.5 | 10.1 | 9.5 | 5.2 | 6.0 | 5.8 | 7.6 | 8.3 | 8.6 |
Std Deviation (1e-4) | 5.5 | 6.9 | 7.0 | 7.6 | 6.7 | 6.1 | 5.2 | 5.5 | 5.0 | 5.1 | 5.2 | 5.3 | 10.1 | 10.2 | 9.2 | 2.3 | 1.7 | 1.8 | 7.7 | 7.9 | 7.5 |
Intensity Change (Log2) | – | – | – | 0.06 | -0.04 | -0.33 | -0.37 | -0.27 | -0.55 | -0.45 | -0.21 | -0.64 | 0.27 | 0.21 | 0.12 | -0.73 | -0.53 | -0.59 | -0.19 | -0.07 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -1.8 | -0.2 | -4.1 | -3.6 | -3.2 | -2.8 | -3.8 | -1.2 | 1.9 | 1.5 | 2.8 | 5.4 | 3.2 | 3.8 |
Endoplasmic Reticulum | 0.6 | -1.0 | -1.2 | 0 | 0 | -0.4 | -0.6 | 0 | 0.5 | -0.1 | 0 | 0 | -1.8 | 0.9 | -0.1 |
Endosome | 0 | 2.7 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.5 | 0.1 | 4.6 | 5.3 | 5.2 | 7.3 | 6.5 | 5.9 | 6.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 3.0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.7 | -0.2 | -3.0 | 0.3 | 1.2 | -2.6 | -2.3 | -0.7 | -2.2 | -1.2 | -1.0 | -1.5 | -3.9 | -1.4 | -3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.3317 | 0.0014 | 0.0002 | 0.0001 | 0.003 | 0.0043 | 0.0623 | 0.0026 | 0.0319 | 0.0026 | 0.0006 | 0.004 | 0.0053 | 0.0055 | 0.0113 | 0.001 | 0.1109 | 0.017 |
Bud | 0.006 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.001 | 0.0002 | 0.0001 | 0.0013 | 0.0006 |
Bud Neck | 0.0023 | 0.0001 | 0.0002 | 0 | 0.001 | 0.002 | 0.0005 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0041 | 0.0007 | 0.0008 | 0.0007 | 0.0001 | 0.0025 | 0.0046 |
Bud Periphery | 0.0148 | 0 | 0 | 0 | 0 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0007 | 0.0005 | 0.0003 | 0 | 0.0011 | 0.0004 |
Bud Site | 0.0056 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0.0008 | 0.0006 | 0.0028 | 0.0003 | 0 | 0.0001 | 0.0054 | 0.0022 | 0.0026 | 0 | 0.0014 | 0.0001 |
Cell Periphery | 0.0015 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0.0002 | 0.0012 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0037 | 0.4059 | 0.4312 | 0.1518 | 0.3367 | 0.2307 | 0.141 | 0.3983 | 0.2905 | 0.2339 | 0.0381 | 0.1121 | 0.1078 | 0.3985 | 0.2605 | 0.5107 | 0.2143 | 0.1677 |
Cytoplasmic Foci | 0.0047 | 0.0315 | 0.0003 | 0 | 0.0031 | 0.0185 | 0.0308 | 0.0258 | 0.0092 | 0.0271 | 0.1405 | 0.0305 | 0.0661 | 0.0261 | 0.0385 | 0.0172 | 0.0307 | 0.0072 |
Eisosomes | 0.0032 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0629 | 0.0215 | 0.033 | 0.019 | 0.0117 | 0.0146 | 0.0483 | 0.0227 | 0.0337 | 0.0219 | 0.1565 | 0.0285 | 0.0791 | 0.023 | 0.0548 | 0.011 | 0.0224 | 0.048 |
Endosome | 0.0837 | 0.0014 | 0.0022 | 0.0014 | 0.0081 | 0.0465 | 0.0324 | 0.0233 | 0.0156 | 0.0532 | 0.0451 | 0.0295 | 0.0925 | 0.0141 | 0.0109 | 0.0183 | 0.0619 | 0.0159 |
Golgi | 0.0387 | 0.0002 | 0 | 0 | 0.0002 | 0.017 | 0.0255 | 0.0058 | 0.0051 | 0.0022 | 0.0066 | 0.0071 | 0.0125 | 0.0013 | 0.0031 | 0.0012 | 0.0153 | 0.0022 |
Lipid Particles | 0.0152 | 0.0003 | 0 | 0 | 0.0002 | 0.0115 | 0.0113 | 0.0025 | 0.0218 | 0.0049 | 0.0737 | 0.0075 | 0.0487 | 0.0051 | 0.0047 | 0.0167 | 0.0161 | 0.0061 |
Mitochondria | 0.0524 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0493 | 0.0055 | 0.0025 | 0.0012 | 0.0012 | 0.0005 | 0.0016 | 0.0044 | 0.0017 | 0.001 | 0.0003 | 0.0057 | 0.0176 |
None | 0.0134 | 0.0015 | 0.0002 | 0.0001 | 0.0007 | 0.0009 | 0.0106 | 0.0005 | 0.0005 | 0.0033 | 0.0003 | 0.0006 | 0.0188 | 0.0005 | 0.0041 | 0.0007 | 0.004 | 0.0023 |
Nuclear Periphery | 0.2897 | 0.4232 | 0.4486 | 0.6104 | 0.4724 | 0.4935 | 0.4866 | 0.4327 | 0.5209 | 0.522 | 0.4926 | 0.6659 | 0.3895 | 0.4122 | 0.5139 | 0.3601 | 0.3868 | 0.5868 |
Nucleolus | 0.0018 | 0.0001 | 0 | 0 | 0.0003 | 0.0012 | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0084 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0002 |
Nucleus | 0.0138 | 0.0877 | 0.0766 | 0.2117 | 0.1409 | 0.0756 | 0.0896 | 0.0546 | 0.0508 | 0.0508 | 0.0306 | 0.087 | 0.0987 | 0.088 | 0.0756 | 0.0274 | 0.0985 | 0.1068 |
Peroxisomes | 0.0025 | 0.0005 | 0 | 0 | 0.0002 | 0.0006 | 0.0024 | 0.0005 | 0.0019 | 0.0003 | 0.0014 | 0.0005 | 0.0017 | 0.0002 | 0.0014 | 0.0001 | 0.0052 | 0.0004 |
Punctate Nuclear | 0.0062 | 0.0202 | 0.0008 | 0.0004 | 0.0126 | 0.0052 | 0.0406 | 0.0163 | 0.0032 | 0.0674 | 0.0048 | 0.0072 | 0.0314 | 0.0106 | 0.005 | 0.0233 | 0.0103 | 0.0063 |
Vacuole | 0.0108 | 0.0017 | 0.0019 | 0.0011 | 0.0028 | 0.0109 | 0.0036 | 0.0036 | 0.0033 | 0.003 | 0.0019 | 0.0041 | 0.0133 | 0.0043 | 0.0038 | 0.0044 | 0.0042 | 0.0032 |
Vacuole Periphery | 0.0355 | 0.0025 | 0.0044 | 0.0038 | 0.005 | 0.0161 | 0.006 | 0.0063 | 0.0061 | 0.0051 | 0.0064 | 0.0089 | 0.0135 | 0.0039 | 0.0063 | 0.0073 | 0.0064 | 0.0063 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 136.1475 | 132.0012 | 141.5341 | 138.0473 | 133.4887 | 108.9931 | 126.5787 | 122.7247 | 125.5343 | 131.7295 |
Translational Efficiency | 1.8165 | 1.9132 | 1.5014 | 1.6801 | 1.6188 | 2.1479 | 1.6921 | 1.6784 | 1.4837 | 1.4896 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1522 | 1123 | 977 | 651 | 1524 | 1635 | 1407 | 124 | 3046 | 2758 | 2384 | 775 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1515.78 | 1789.05 | 1982.62 | 2730.69 | 1830.34 | 1923.96 | 2473.01 | 2909.76 | 1673.16 | 1869.03 | 2272.04 | 2759.34 |
Standard Deviation | 237.27 | 448.03 | 399.25 | 761.88 | 303.89 | 364.09 | 642.07 | 706.41 | 314.76 | 405.85 | 605.63 | 756.13 |
Intensity Change Log 2 | — | 0.239133 | 0.387348 | 0.849205 | — | 0.071967 | 0.434156 | 0.668788 | — | 0.150100 | 0.413140 | 0.753321 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000436 | 0.000378 | 0.000800 | 0.002806 | 0.000227 | 0.000203 | 0.000658 | 0.009245 | 0.000331 | 0.000274 | 0.000716 | 0.003836 |
Bud Neck | 0.009014 | 0.006761 | 0.022439 | 0.041265 | 0.005031 | 0.005508 | 0.027523 | 0.035181 | 0.007021 | 0.006018 | 0.025439 | 0.040292 |
Bud Site | 0.011406 | 0.005039 | 0.007129 | 0.050244 | 0.002184 | 0.003067 | 0.011106 | 0.025207 | 0.006792 | 0.003870 | 0.009476 | 0.046238 |
Cell Periphery | 0.000152 | 0.000276 | 0.000329 | 0.000640 | 0.000094 | 0.000101 | 0.000293 | 0.000366 | 0.000123 | 0.000172 | 0.000308 | 0.000596 |
Cytoplasm | 0.170942* | 0.264160* | 0.163524* | 0.070780 | 0.135989* | 0.130066* | 0.130241* | 0.048090 | 0.153454* | 0.184666* | 0.143881* | 0.067150 |
Cytoplasmic Foci | 0.028270 | 0.011068 | 0.017570 | 0.111616 | 0.009921 | 0.007043 | 0.017190 | 0.100910 | 0.019089 | 0.008682 | 0.017345 | 0.109903 |
Eisosomes | 0.000009 | 0.000005 | 0.000014 | 0.000055 | 0.000003 | 0.000006 | 0.000018 | 0.000028 | 0.000006 | 0.000005 | 0.000016 | 0.000051 |
Endoplasmic Reticulum | 0.003268 | 0.001952 | 0.003294 | 0.001155 | 0.006573 | 0.002523 | 0.001608 | 0.000693 | 0.004921 | 0.002291 | 0.002299 | 0.001081 |
Endosome | 0.013703 | 0.004950 | 0.004370 | 0.029266 | 0.007951 | 0.004624 | 0.006029 | 0.014933 | 0.010825 | 0.004757 | 0.005349 | 0.026973 |
Golgi | 0.005848 | 0.003999 | 0.001992 | 0.028213 | 0.002252 | 0.001968 | 0.002863 | 0.028145 | 0.004049 | 0.002795 | 0.002506 | 0.028202 |
Lipid Particles | 0.003192 | 0.001713 | 0.002335 | 0.013890 | 0.002500 | 0.000955 | 0.001384 | 0.010519 | 0.002846 | 0.001264 | 0.001774 | 0.013350 |
Mitochondria | 0.004831 | 0.001592 | 0.004658 | 0.024844 | 0.001706 | 0.002285 | 0.005408 | 0.027416 | 0.003267 | 0.002003 | 0.005101 | 0.025256 |
Mitotic Spindle | 0.096363 | 0.045371 | 0.028754 | 0.227157* | 0.063268 | 0.073403 | 0.101272* | 0.269451* | 0.079805 | 0.061989 | 0.071553 | 0.233924* |
None | 0.010640 | 0.016990 | 0.012267 | 0.005839 | 0.004103 | 0.005093 | 0.014471 | 0.002125 | 0.007369 | 0.009937 | 0.013568 | 0.005245 |
Nuclear Periphery | 0.071718 | 0.079315 | 0.127897* | 0.043245 | 0.143706* | 0.197980* | 0.172365* | 0.074461 | 0.107736* | 0.149662* | 0.154141* | 0.048240 |
Nuclear Periphery Foci | 0.433024* | 0.454177* | 0.131707 | 0.171338 | 0.551891* | 0.448595* | 0.177106 | 0.156153 | 0.492497* | 0.450868* | 0.158501 | 0.168908 |
Nucleolus | 0.018863 | 0.008343 | 0.026823 | 0.017802 | 0.003557 | 0.007935 | 0.029400 | 0.015507 | 0.011205 | 0.008101 | 0.028344 | 0.017435 |
Nucleus | 0.073119 | 0.062021 | 0.412929* | 0.045643 | 0.034792 | 0.084140 | 0.273414* | 0.059852 | 0.053943 | 0.075134 | 0.330590* | 0.047917 |
Peroxisomes | 0.001199 | 0.000414 | 0.001607 | 0.007490 | 0.000134 | 0.000377 | 0.001538 | 0.005808 | 0.000666 | 0.000392 | 0.001566 | 0.007221 |
Vacuole | 0.027831 | 0.023205 | 0.023429 | 0.073354 | 0.017900 | 0.016225 | 0.018032 | 0.073296 | 0.022862 | 0.019067 | 0.020244 | 0.073345 |
Vacuole Periphery | 0.016173 | 0.008270 | 0.006132 | 0.033357 | 0.006219 | 0.007904 | 0.008081 | 0.042613 | 0.011192 | 0.008053 | 0.007282 | 0.034838 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.73 | -4.71 | -4.94 | -5.09 | -3.01 | 0.82 | -3.01 | -1.10 | -1.11 | -0.95 | 1.49 | -4.60 | -3.15 | -3.24 | -2.28 |
Bud Neck | 1.85 | -11.02 | -14.51 | -14.92 | -6.81 | -1.71 | -14.52 | -6.70 | -6.53 | -2.90 | 0.47 | -17.94 | -16.51 | -16.57 | -6.99 |
Bud Site | 4.24 | 1.32 | -9.63 | -11.37 | -10.23 | -1.14 | -9.83 | -5.01 | -4.81 | -2.77 | 3.44 | -5.02 | -11.67 | -12.53 | -10.12 |
Cell Periphery | -5.05 | -6.07 | -11.61 | -8.11 | -5.70 | -0.23 | -7.11 | -6.30 | -5.97 | -2.89 | -3.01 | -9.09 | -13.42 | -11.51 | -7.46 |
Cytoplasm | -7.23 | 1.96 | 10.38 | 15.80 | 8.38 | 0.10 | -0.15 | 4.28 | 4.29 | 4.48 | -4.26 | 1.75 | 10.33 | 13.57 | 9.21 |
Cytoplasmic Foci | 5.65 | 2.72 | -9.62 | -12.47 | -10.97 | 2.01 | -3.14 | -4.18 | -4.43 | -3.62 | 6.20 | 0.57 | -11.78 | -13.70 | -11.92 |
Eisosomes | 3.91 | -4.15 | -7.28 | -8.08 | -5.73 | -1.49 | -8.23 | -4.57 | -3.76 | -1.31 | 0.52 | -8.69 | -8.47 | -8.38 | -5.65 |
Endoplasmic Reticulum | 3.68 | -0.48 | 6.83 | 4.22 | 8.09 | 5.61 | 7.04 | 8.48 | 7.22 | 4.87 | 6.42 | 6.35 | 10.10 | 7.22 | 7.85 |
Endosome | 9.22 | 9.94 | -4.79 | -8.89 | -9.15 | 4.69 | 2.68 | -2.28 | -4.31 | -3.54 | 10.09 | 8.62 | -6.15 | -9.53 | -9.19 |
Golgi | 2.56 | 6.25 | -5.34 | -6.00 | -6.76 | 0.94 | -1.19 | -2.29 | -2.34 | -2.21 | 3.24 | 4.28 | -6.32 | -6.82 | -6.96 |
Lipid Particles | 2.29 | 0.62 | -6.77 | -7.97 | -6.76 | 4.31 | 2.78 | -2.22 | -2.88 | -2.69 | 4.58 | 2.11 | -7.42 | -8.77 | -8.11 |
Mitochondria | 4.08 | 0.02 | -4.27 | -5.36 | -4.36 | -1.69 | -6.24 | -1.96 | -1.88 | -1.48 | 2.74 | -3.59 | -5.11 | -5.55 | -4.49 |
Mitotic Spindle | 7.03 | 9.64 | -9.36 | -13.17 | -14.44 | -1.71 | -5.71 | -6.56 | -6.21 | -5.13 | 3.37 | 0.41 | -12.34 | -13.69 | -12.32 |
None | -2.66 | -0.20 | 3.69 | 5.11 | 3.38 | -1.51 | -5.42 | 0.25 | 1.57 | 5.38 | -2.28 | -4.07 | 1.78 | 3.55 | 5.04 |
Nuclear Periphery | -1.67 | -5.28 | 5.69 | 7.01 | 9.02 | -6.37 | 0.15 | 7.19 | 11.04 | 6.75 | -7.83 | -3.97 | 13.33 | 19.56 | 14.64 |
Nuclear Periphery Foci | -1.86 | 25.26* | 20.53* | 20.65* | -1.66 | 9.40 | 36.90* | 23.09* | 17.16 | 2.23 | 4.83 | 42.91 | 32.12 | 27.54* | 0.07 |
Nucleolus | 5.89 | -3.25 | 0.26 | -4.32 | 3.07 | -5.08 | -12.05 | -3.52 | -2.44 | 1.71 | 2.51 | -10.24 | -3.55 | -4.91 | 4.30 |
Nucleus | 2.12 | -27.99 | 1.38 | -0.31 | 27.10 | -10.83 | -27.73 | -4.10 | -1.31 | 7.52 | -6.28 | -38.05 | -2.83 | 1.02 | 28.51 |
Peroxisomes | 3.09 | -0.74 | -6.38 | -7.78 | -5.59 | -1.69 | -5.39 | -3.13 | -2.95 | -1.92 | 2.05 | -3.69 | -7.84 | -8.38 | -6.13 |
Vacuole | 1.34 | -0.06 | -10.99 | -11.26 | -10.62 | 0.35 | -2.07 | -5.35 | -5.42 | -4.94 | 1.67 | -1.12 | -13.01 | -13.50 | -12.52 |
Vacuole Periphery | 4.98 | 7.37 | -2.91 | -5.10 | -5.96 | -1.41 | -1.10 | -3.28 | -3.04 | -3.10 | 3.26 | 4.95 | -4.94 | -5.94 | -6.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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