Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 – 0 0 0.05 0.08 0.06 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.71 0.43 0.37 0.28 0.28 0.17 – 0.11 0.12 0.15 0.12 0.11 0.68 0.68 0.75 0.87 – – 0.29 0 0.07 0 0 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0 0.05 0 – – 0 0 0 0 0 0
Endosome 0 0.07 0 0 0.11 0 – 0 0 0 0 0 0 0.12 0.11 0 – – 0.07 0.08 0.09 0.07 0.08 0.06
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.27 0.4 0.65 0.81 0.62 0.81 – 0.87 0.91 0.89 0.88 0.91 0.14 0.15 0.05 0.12 – – 0.4 0.67 0.64 0.68 0.63 0.5
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0.06 0 0 0.05 0 – 0.06 0.05 0.05 0.08 0.05 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0 0 0 – 0 0 0 0 0 0.05 0.08 0.07 0 – – 0.09 0 0 0 0.08 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 – 0 0 0 0 0 0 1 0 0 – – 5 9 12 3 3 11
Bud 1 3 13 8 5 8 – 14 16 25 40 34 1 2 0 1 – – 1 5 3 2 2 7
Bud Neck 1 0 5 0 0 0 – 3 0 1 1 0 0 0 0 2 – – 4 1 2 0 0 8
Bud Site 0 2 1 1 2 7 – 8 8 14 6 14 0 0 0 0 – – – – – – – –
Cell Periphery 1 6 2 2 1 0 – 3 0 2 4 4 1 0 1 2 – – 1 0 2 0 0 0
Cytoplasm 109 73 107 64 65 60 – 57 51 71 65 63 67 89 98 59 – – 59 10 23 2 6 20
Endoplasmic Reticulum 3 4 4 0 3 2 – 1 1 0 2 0 8 0 6 3 – – 2 3 2 0 0 1
Endosome 3 11 0 1 25 6 – 2 0 0 2 2 3 16 15 3 – – 15 18 30 6 11 12
Golgi 0 1 1 1 1 0 – 0 0 0 0 0 1 1 0 1 – – 0 4 2 1 2 3
Mitochondria 41 67 191 189 144 277 – 435 384 432 460 506 14 19 6 8 – – 81 160 217 64 93 104
Nucleus 1 0 1 1 1 9 – 8 5 8 7 4 1 0 0 0 – – 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 2 – 3 1 2 0 0 0 0 1 0 – – 0 1 2 0 1 0
Nucleolus 1 0 1 1 2 2 – 3 0 3 3 2 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 2 1 6 12 3 – 4 0 2 8 5 0 2 5 0 – – 0 4 10 3 4 1
SpindlePole 5 10 12 4 12 10 – 29 22 22 42 30 2 1 2 0 – – 6 5 8 2 5 6
Vac/Vac Membrane 4 17 6 4 6 14 – 10 8 8 6 11 5 10 9 0 – – 17 9 13 2 11 25
Unique Cell Count 154 168 292 232 233 343 501 422 486 521 554 99 130 131 68 204 239 342 96 148 209
Labelled Cell Count 170 197 346 282 279 400 580 496 590 646 675 103 141 143 79 204 239 342 96 148 209


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.9 3.3 3.4 4.1 3.4 – 3.3 2.9 3.2 3.0 3.1 4.7 5.0 4.7 5.5 – 4.8 4.4 5.1 5.0
Std Deviation (1e-4) 0.4 1.6 0.9 1.8 1.7 1.6 – 1.0 1.1 1.0 1.3 1.0 0.9 1.5 0.9 2.0 – 1.2 0.5 1.2 0.9
Intensity Change (Log2) – – – 0.03 0.3 0.05 – -0.0 -0.19 -0.06 -0.13 -0.12 0.5 0.6 0.5 0.72 – 0.53 0.39 0.62 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Cytoplasm -2.2 -2.1 -5.5 – -8.5 -7.8 -7.1 -8.1 -8.7 5.4 6.1 7.3 7.5 – –
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Mitochondria 0 0 0 – 0 0 0 0 0 0 -9.6 0 -8.0 – –
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 – –
Vacuole 0 0.4 1.5 – -0.1 -0.2 -0.4 0 -0.1 0 0 0 0 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1244 0.2657 0.144 -0.1609 -0.312 0.1111 -0.5189 0.1228 -0.1427 -0.1574 -0.2414 -0.0512 2.3898 2.3924 3.0986 2.3592 2.8282 2.2998
Actin 0.003 0.0102 0.0078 0.0042 0.0093 0.0045 0.0082 0.0116 0.0047 0.0088 0.0181 0.0083 0.0075 0.0093 0.0031 0.0019 0.0073 0.0036
Bud 0.0037 0.0036 0.0031 0.0079 0.0024 0.0028 0.0019 0.0044 0.0031 0.0029 0.0024 0.0018 0.0014 0.006 0.0037 0.0014 0.0041 0.0019
Bud Neck 0.0034 0.0018 0.0023 0.0068 0.0014 0.0123 0.0058 0.0027 0.0031 0.0029 0.0036 0.0085 0.0079 0.0016 0.0038 0.0019 0.002 0.0033
Bud Periphery 0.0041 0.0088 0.0068 0.0226 0.0079 0.0044 0.0056 0.0079 0.0076 0.0097 0.0064 0.0043 0.0048 0.0187 0.011 0.0025 0.0078 0.0056
Bud Site 0.0068 0.0447 0.0117 0.0247 0.0018 0.0025 0.0089 0.0455 0.0166 0.0029 0.0065 0.0021 0.0081 0.0234 0.0239 0.0014 0.0044 0.0017
Cell Periphery 0.0007 0.0029 0.0012 0.0013 0.0004 0.0007 0.0011 0.0016 0.0016 0.0013 0.0006 0.0005 0.0016 0.0011 0.0011 0.0002 0.0079 0.0009
Cytoplasm 0.0451 0.0529 0.0591 0.0259 0.0538 0.0502 0.0566 0.0454 0.0617 0.057 0.0515 0.0628 0.0636 0.0645 0.0465 0.0204 0.0393 0.0509
Cytoplasmic Foci 0.1588 0.1826 0.1371 0.1554 0.1603 0.1765 0.1628 0.1305 0.1495 0.1291 0.1392 0.1402 0.1182 0.1453 0.1477 0.2042 0.1503 0.1009
Eisosomes 0.0005 0.0007 0.0006 0.0005 0.0003 0.0003 0.0009 0.0005 0.001 0.0003 0.0004 0.0004 0.0011 0.0004 0.0004 0.0001 0.006 0.0003
Endoplasmic Reticulum 0.0027 0.003 0.0027 0.0034 0.0026 0.0021 0.0037 0.0033 0.0035 0.002 0.0019 0.0027 0.0045 0.0021 0.0026 0.0012 0.0047 0.0014
Endosome 0.1757 0.1416 0.1184 0.1356 0.1444 0.1278 0.1867 0.162 0.1142 0.1037 0.1317 0.1621 0.1759 0.1701 0.1464 0.177 0.1419 0.1353
Golgi 0.0567 0.0497 0.0353 0.0329 0.0539 0.0466 0.0509 0.0726 0.0418 0.0403 0.0454 0.0538 0.0579 0.0531 0.0512 0.0525 0.0367 0.048
Lipid Particles 0.0346 0.019 0.0196 0.0377 0.0486 0.0383 0.031 0.0115 0.0229 0.0082 0.0161 0.0318 0.0293 0.018 0.018 0.023 0.0138 0.0215
Mitochondria 0.2648 0.253 0.3456 0.3178 0.2232 0.27 0.2554 0.2962 0.2914 0.421 0.3178 0.295 0.3362 0.3156 0.3053 0.2921 0.334 0.3874
None 0.1479 0.1318 0.1667 0.1363 0.1758 0.133 0.1448 0.1366 0.171 0.0915 0.1551 0.1294 0.0993 0.0904 0.1288 0.0502 0.1327 0.151
Nuclear Periphery 0.0019 0.0014 0.0019 0.0026 0.0043 0.0011 0.0021 0.001 0.0024 0.0006 0.0012 0.002 0.002 0.0011 0.0029 0.0004 0.0026 0.0005
Nucleolus 0.0034 0.0031 0.0033 0.0044 0.0059 0.0026 0.0014 0.002 0.0024 0.0007 0.0016 0.0014 0.0013 0.0036 0.0056 0.0003 0.001 0.0007
Nucleus 0.0029 0.003 0.0041 0.0044 0.0082 0.0028 0.0025 0.0024 0.003 0.0014 0.0029 0.0028 0.0022 0.0025 0.007 0.0005 0.0022 0.0014
Peroxisomes 0.0603 0.0697 0.0545 0.0487 0.0779 0.1039 0.0501 0.0427 0.0827 0.0951 0.0798 0.071 0.0616 0.0547 0.0679 0.1611 0.0828 0.0739
Punctate Nuclear 0.0054 0.0019 0.0046 0.015 0.0069 0.007 0.0024 0.0014 0.0035 0.0009 0.0061 0.0036 0.001 0.0055 0.0051 0.001 0.0018 0.0011
Vacuole 0.0138 0.0116 0.0106 0.0084 0.0074 0.0075 0.0129 0.0149 0.0093 0.0147 0.0082 0.0109 0.0101 0.0089 0.0134 0.0042 0.0124 0.0056
Vacuole Periphery 0.0039 0.0031 0.0032 0.0035 0.0035 0.003 0.0041 0.0033 0.0031 0.005 0.0035 0.0046 0.0045 0.0041 0.0045 0.0024 0.0044 0.0031

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
44 1568 168 1427 1966 951 1066 1531 2010 2519 1234 2958

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 650.80 715.96 910.94 825.45 660.47 711.22 789.23 836.55 660.26 714.17 805.80 831.20
Standard Deviation 111.68 89.63 107.63 103.50 77.37 79.26 117.73 113.98 78.29 85.89 123.66 109.19
Intensity Change Log 2 — 0.137665 0.485142 0.342967 — 0.106803 0.256953 0.340959 — 0.122203 0.374714 0.341956

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000775 0.001275 0.000294 0.010483 0.000926 0.000635 0.005982 0.009797 0.000923 0.001033 0.005207 0.010128
Bud Neck 0.024774 0.044159 0.005104 0.031425 0.041698 0.067920 0.028026 0.037546 0.041328 0.053130 0.024906 0.034593
Bud Site 0.018169 0.030235 0.007852 0.098118 0.010465 0.017408 0.112409* 0.108161* 0.010633 0.025392 0.098174 0.103316*
Cell Periphery 0.000784 0.000266 0.000316 0.000288 0.000317 0.000298 0.000253 0.000238 0.000327 0.000278 0.000262 0.000262
Cytoplasm 0.282272* 0.089099 0.249327* 0.135421* 0.141275* 0.110844* 0.230547* 0.128965* 0.144362* 0.097309 0.233104* 0.132080*
Cytoplasmic Foci 0.263975* 0.290580* 0.108016 0.160953 0.363487* 0.315458* 0.145530 0.166637 0.361309* 0.299972* 0.140423 0.163895
Eisosomes 0.000864 0.000272 0.000182 0.000189 0.000436 0.000339 0.000123 0.000173 0.000445 0.000297 0.000131 0.000181
Endoplasmic Reticulum 0.001284 0.000982 0.004013 0.004486 0.002276 0.000887 0.001990 0.002000 0.002254 0.000946 0.002265 0.003200
Endosome 0.021516 0.077578 0.020996 0.063330 0.052465 0.093884 0.065406 0.065708 0.051788 0.083734 0.059360 0.064561
Golgi 0.010156 0.073664 0.000470 0.032804 0.037773 0.041920 0.032672 0.038076 0.037168 0.061680 0.028288 0.035532
Lipid Particles 0.045999 0.012789 0.010935 0.005263 0.037788 0.019573 0.006048 0.004034 0.037968 0.015350 0.006713 0.004627
Mitochondria 0.054486 0.216071* 0.000676 0.062366 0.171748* 0.158394* 0.061369 0.068139 0.169181* 0.194296* 0.053106 0.065354
Mitotic Spindle 0.007914 0.013774 0.002723 0.074273 0.003081 0.006141 0.063098 0.078941 0.003187 0.010892 0.054879 0.076689
None 0.010797 0.001132 0.001837 0.000568 0.001385 0.000691 0.001724 0.001410 0.001591 0.000966 0.001739 0.001004
Nuclear Periphery 0.000414 0.000279 0.002005 0.000893 0.000829 0.000245 0.000702 0.000344 0.000819 0.000266 0.000879 0.000609
Nuclear Periphery Foci 0.000280 0.000317 0.003437 0.001187 0.000229 0.000199 0.001004 0.000672 0.000230 0.000272 0.001335 0.000921
Nucleolus 0.002826 0.001570 0.003710 0.001657 0.001929 0.001424 0.001720 0.001863 0.001949 0.001515 0.001991 0.001764
Nucleus 0.151361* 0.013177 0.394107* 0.039050 0.039301 0.026442 0.042573 0.025609 0.041754 0.018185 0.090432 0.032093
Peroxisomes 0.061893 0.090930 0.002541 0.089515 0.046504 0.080280 0.063177 0.135371* 0.046841 0.086909 0.054921 0.113249*
Vacuole 0.036810 0.033863 0.178247 0.180211 0.037918 0.048307 0.126626 0.120807 0.037894 0.039316 0.133654 0.149465
Vacuole Periphery 0.002653 0.007988 0.003213 0.007519 0.008169 0.008711 0.009022 0.005508 0.008048 0.008261 0.008231 0.006478

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 1.65 -7.71 -7.41 -8.30 0.63 -4.45 -7.86 -8.99 -2.75 -0.25 -4.24 -9.88 -11.42 -4.16
Bud Neck -1.51 1.38 -0.59 4.14 -10.91 -5.24 5.11 1.49 6.29 -4.01 -3.70 6.34 2.68 6.99 -5.12
Bud Site -1.49 1.25 -7.88 -13.40 -13.78 -4.87 -16.27 -22.13 -19.80 0.51 -10.94 -15.71 -28.79 -22.60 -0.70
Cell Periphery 2.25 2.00 2.32 0.54 1.52 0.49 2.15 2.34 1.82 0.40 1.34 2.18 2.46 1.82 0.35
Cytoplasm 4.51 0.60 3.09 -8.75 5.72 4.38 -11.05 0.55 -3.60 10.99 8.92 -11.62 0.45 -9.24 12.28
Cytoplasmic Foci -0.77 5.35 4.07 21.23 -4.66 5.24 31.80 31.60 18.95 -3.58 9.04 34.04 35.91 28.30 -4.08
Eisosomes 1.49 1.73 1.75 5.45 0.52 2.53 14.81 12.40 5.08 -4.97 5.60 14.19 12.20 7.57 -5.22
Endoplasmic Reticulum 0.52 -4.61 -6.20 -6.85 0.29 3.48 -0.06 0.14 -4.85 0.29 3.08 -0.96 -2.61 -7.61 -2.47
Endosome -6.12 0.04 -3.71 5.92 -9.43 -8.26 -1.56 -1.82 6.59 -0.05 -10.08 -0.43 -1.91 8.62 -1.06
Golgi -14.68 2.91 -4.19 13.41 -12.34 -1.22 3.41 2.26 2.87 -1.39 -9.11 4.90 3.74 12.80 -1.99
Lipid Particles 2.92 3.05 3.55 6.18 2.61 7.07 17.17 18.45 7.75 2.03 11.35 17.13 18.82 10.00 2.45
Mitochondria -7.62 2.58 0.12 21.39 -14.15 1.48 17.05 17.27 11.89 -1.03 -3.28 18.72 19.07 24.24 -2.02
Mitotic Spindle -1.58 1.39 -11.54 -12.38 -14.20 -2.21 -13.06 -16.23 -15.22 -2.46 -6.34 -12.80 -21.99 -19.08 -4.37
None 1.10 1.02 1.16 2.50 3.91 1.93 -0.47 0.04 -1.26 0.43 1.61 -0.17 1.35 -0.08 1.45
Nuclear Periphery 1.11 -6.16 -6.10 -5.58 3.25 3.66 -0.55 1.69 -4.37 3.43 3.16 -1.58 -0.36 -5.87 2.04
Nuclear Periphery Foci -0.22 -6.63 -6.97 -6.93 4.51 0.71 -5.73 -6.66 -6.65 2.49 -1.11 -7.90 -10.28 -8.99 2.97
Nucleolus 1.13 0.10 1.02 -0.88 2.15 1.50 0.54 -0.07 -1.69 -0.67 1.35 -0.07 0.23 -1.70 0.42
Nucleus 4.39 -6.01 3.24 -11.81 15.14 4.13 -3.40 2.10 -2.13 4.82 9.85 -9.73 0.48 -10.70 10.19
Peroxisomes -1.48 3.30 -0.71 2.70 -18.66 -6.74 -2.08 -14.16 -6.12 -10.26 -11.46 -0.43 -14.02 -2.95 -10.67
Vacuole 0.69 -6.59 -10.05 -28.37 -1.36 -3.39 -17.98 -21.97 -18.57 -0.92 -0.74 -20.08 -34.10 -33.84 -4.35
Vacuole Periphery -5.95 -0.18 -3.92 2.39 -2.30 -0.67 0.38 4.38 4.19 2.97 -0.36 1.11 4.49 4.67 2.08
Standard name
Human Ortholog
Description Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
Localization
Cell Percentages mitochondrion (53%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrx7

Mrx7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available