Standard name
Human Ortholog
Description Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0.08 0.06 0.14 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0.08 0.11 0.05 0.16 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.09 0.08 0.09 0.09 0.1 – 0.09 0.12 0.1 0 0.05 0.08 0.24 0.25 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0.16 0.28 0.26 0.11 0.12 0.18 0.19 0.25 – 0.2 0.16 0.23 0.24 0.43 0.36 0 0.05 0.07 0.37 0.41 0.66 0.71 0.79 0.78
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.23 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.12 0.06 0.18 0.17 0.21 – 0.36 0.43 0.39 0 0 0 0.07 0.12 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.65 0.44 0.58 0.56 0.41 0.31 0.28 0.22 – 0.17 0.16 0.21 0.46 0.34 0.17 0.28 0.27 0.2 0.53 0.43 0.26 0.19 0.11 0.07
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.47 0.26 0.28 0.46 0.4 0.39 0.39 – 0.33 0.27 0.23 0.46 0.36 0.48 0.26 0.36 0.14 0 0.09 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 3 4 1 5 15 – 8 6 18 7 18 21 6 5 6 0 0 2 4 7 5
Bud 0 0 0 1 1 3 1 3 – 8 4 9 1 3 0 1 0 0 2 3 0 4 3 6
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 1 0 0 – 2 1 1 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 4 4 10 1 3 4 – 12 12 20 14 36 65 8 4 7 0 0 0 0 0 0
Cytoplasm 4 4 20 28 28 25 30 44 – 35 40 36 12 45 70 17 21 8 0 0 0 0 0 3
Endoplasmic Reticulum 23 25 63 33 42 47 63 115 – 77 56 84 147 357 314 2 4 3 62 54 176 276 396 465
Endosome 0 0 3 2 1 2 6 2 – 3 2 4 0 2 4 0 0 0 0 0 1 2 4 4
Golgi 0 0 1 3 7 1 0 3 – 2 0 0 10 17 8 0 5 10 1 0 1 3 3 5
Mitochondria 2 4 18 37 21 47 56 95 – 137 149 142 1 0 1 5 10 6 3 1 5 10 12 9
Nucleus 2 0 1 6 5 2 3 11 – 9 11 5 13 18 13 4 1 1 1 0 0 0 0 1
Nuclear Periphery 95 40 140 170 146 83 94 101 – 65 54 77 283 283 144 20 23 9 90 56 67 73 54 44
Nucleolus 1 0 2 1 1 0 2 4 – 3 0 2 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 – 0 0 0 1 1 1 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 75 42 62 84 161 106 129 176 – 125 92 82 280 300 415 19 30 6 5 11 6 8 18 42
Unique Cell Count 147 90 243 304 353 266 332 455 384 343 364 611 829 861 72 84 44 171 133 266 388 505 594
Labelled Cell Count 204 115 314 372 427 319 393 574 486 427 480 770 1080 1058 82 103 56 171 133 266 388 505 594


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 11.8 10.6 11.3 11.7 10.5 10.2 9.9 – 8.9 9.0 9.2 13.4 12.3 13.4 10.1 11.3 15.2 13.8 15.2 15.6
Std Deviation (1e-4) 2.3 2.2 2.2 2.4 2.6 2.3 2.2 2.1 – 1.9 2.0 2.1 2.2 2.0 2.3 2.4 2.7 3.3 2.6 2.9 2.7
Intensity Change (Log2) – – – 0.1 0.14 -0.0 -0.04 -0.09 – -0.25 -0.24 -0.2 0.34 0.21 0.35 -0.06 0.09 0.52 0.39 0.52 0.56

WT3RAP60RAP140RAP220RAP300RAP380RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.9 – 0 0 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.9 0 0 0 – 1.1 1.4 2.4 0.6 2.0 3.4 0 0 0
Cytoplasm 0.4 -0.1 0.5 0.3 0.6 – 0.4 1.3 0.7 -4.4 -1.6 -0.1 3.6 4.0 0
Endoplasmic Reticulum -4.6 -4.4 -2.3 -2.0 -0.2 – -1.7 -2.8 -0.8 -0.6 4.8 3.1 -4.3 -4.1 -2.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 -0.7 3.5 3.3 4.6 – 8.0 9.5 8.7 -6.5 0 0 -0.1 1.3 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 -3.9 -6.0 -7.1 -9.4 – -10.6 -10.6 -9.2 -3.0 -6.6 -12.9 -4.4 -4.8 -4.5
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0.6 5.0 3.4 3.4 3.5 – 1.9 0.4 -0.8 5.5 3.1 6.3 0.1 1.8 -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.5379 14.0675 13.8725 12.8501 11.8643 13.2849 16.6412 15.3933 14.0771 14.2509 14.6686 14.0952 16.3561 14.9628 15.0644 14.6327 13.7943 14.173
Actin 0.0131 0.0001 0.0092 0.0178 0.0022 0.001 0.0197 0 0.0051 0.0002 0.0166 0.0019 0.0012 0.0011 0.004 0.0005 0.0036 0.0003
Bud 0.0002 0 0.0001 0.0003 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0
Bud Neck 0.0053 0.0001 0.0006 0.0014 0.0007 0.0001 0.0002 0 0.0002 0.0001 0.0005 0 0 0.0001 0.0001 0.0001 0.0011 0
Bud Periphery 0.0003 0 0.0002 0.0007 0.0007 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0.0005 0.0003 0.0004 0.0002 0
Bud Site 0.0029 0 0.0008 0.0015 0.0012 0 0.0006 0 0.0005 0 0.0005 0 0.0001 0.0001 0.0001 0 0.0011 0
Cell Periphery 0.0003 0.0003 0.0016 0.0006 0.0006 0 0.0004 0.0002 0.0004 0.0002 0.0006 0.0001 0.0002 0.0076 0.0007 0.0003 0.0001 0
Cytoplasm 0.022 0.0036 0.0015 0.0063 0.0013 0.0019 0.0155 0.0001 0.0031 0.0003 0.0091 0.0001 0.0003 0.0079 0.0002 0.0002 0.0054 0.0001
Cytoplasmic Foci 0.0126 0.0001 0.0003 0.0071 0.003 0.0011 0.0034 0 0.0159 0 0.0304 0.0012 0.0035 0.0002 0.001 0 0.0123 0.0032
Eisosomes 0.0003 0 0.0006 0.0001 0.0019 0 0.0001 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0964 0.3155 0.2833 0.3306 0.2253 0.073 0.1152 0.3835 0.2995 0.3919 0.3424 0.125 0.0789 0.3235 0.3927 0.3985 0.204 0.1037
Endosome 0.0221 0.0011 0.0015 0.0081 0.0069 0.0048 0.0023 0.0002 0.0139 0.0007 0.0056 0.0016 0.0075 0.0025 0.001 0.0011 0.0207 0.0003
Golgi 0.0038 0.0001 0.0006 0.0026 0.003 0.0004 0.0043 0 0.0074 0 0.0021 0.001 0.0055 0.0001 0.0013 0.0001 0.0093 0.0002
Lipid Particles 0.0096 0.0009 0.003 0.0098 0.015 0.0014 0.0054 0.0001 0.0069 0.0001 0.0019 0.0013 0.0092 0.0004 0.002 0.0001 0.0212 0.0005
Mitochondria 0.002 0.0002 0.0005 0.0014 0.0347 0.0002 0.0012 0.0002 0.0008 0.0008 0.0004 0.0003 0.0025 0.0006 0.0043 0.0009 0.0025 0.0001
None 0.0051 0 0.0055 0.0011 0.0526 0.0004 0.0037 0 0.0016 0 0.0018 0.0001 0.0001 0.0002 0.0001 0.0001 0.001 0
Nuclear Periphery 0.7462 0.6131 0.6137 0.5596 0.5577 0.8636 0.7693 0.5564 0.5703 0.5498 0.527 0.8238 0.8298 0.5755 0.5258 0.5463 0.6414 0.848
Nucleolus 0.0008 0.0004 0.0008 0.0001 0.0036 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0.0001 0 0 0.0007 0
Nucleus 0.0123 0.0291 0.0408 0.0223 0.029 0.0231 0.0047 0.0019 0.011 0.004 0.0019 0.0016 0.0075 0.0076 0.004 0.0055 0.0078 0.0033
Peroxisomes 0.0018 0 0.0001 0.0005 0.0159 0.001 0.0026 0 0.001 0 0.0045 0.0009 0.0004 0 0.0048 0 0.0114 0.0028
Punctate Nuclear 0.0145 0.0004 0.0018 0.0014 0.0011 0.004 0.0003 0 0.0027 0 0.0236 0.0004 0.0002 0.0004 0.0001 0 0.0132 0.0045
Vacuole 0.0045 0.0098 0.0092 0.0041 0.0108 0.0027 0.0034 0.0047 0.0071 0.0053 0.0037 0.0018 0.0034 0.0201 0.0064 0.0056 0.0062 0.0015
Vacuole Periphery 0.0239 0.0251 0.0242 0.0224 0.0327 0.0211 0.0474 0.0525 0.0524 0.0465 0.0268 0.039 0.0494 0.0513 0.0511 0.0402 0.0365 0.0313

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.8592 57.3612 43.0033 53.5475 51.4609 40.9911 47.6838 47.0139 38.2332 58.4515
Translational Efficiency 0.7032 0.6372 0.7373 0.4651 0.5448 1.0422 0.7234 0.6317 0.7339 0.5276

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 1014 253 117 213 2009 2021 1359 299 3023 2274 1476

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1333.86 1164.03 1524.61 1405.51 1120.46 1257.04 1385.23 1215.29 1181.84 1225.84 1400.74 1230.37
Standard Deviation 163.81 232.01 239.85 232.77 214.32 217.25 212.94 203.77 223.09 226.61 220.50 212.53
Intensity Change Log 2 — -0.196479 0.192833 0.075486 — 0.165939 0.306034 0.117209 — -0.019679 0.245616 0.094684

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002437 0.000471 0.000808 0.000463 0.000368 0.000339 0.000236 0.001249 0.000963 0.000383 0.000300 0.001187
Bud Neck 0.003243 0.000910 0.004089 0.004644 0.001424 0.000614 0.000985 0.003269 0.001947 0.000713 0.001330 0.003378
Bud Site 0.009319 0.002659 0.001521 0.003809 0.003423 0.000987 0.001221 0.005582 0.005119 0.001548 0.001255 0.005442
Cell Periphery 0.001795 0.000885 0.000413 0.000629 0.000384 0.000298 0.000232 0.000549 0.000789 0.000495 0.000252 0.000555
Cytoplasm 0.013534 0.009067 0.001775 0.014207 0.007623 0.007439 0.010776 0.023230 0.009323 0.007985 0.009775 0.022514
Cytoplasmic Foci 0.015024 0.002022 0.000710 0.003033 0.002981 0.001300 0.000549 0.004955 0.006445 0.001542 0.000567 0.004803
Eisosomes 0.000177 0.000008 0.000240 0.000058 0.000016 0.000006 0.000005 0.000047 0.000062 0.000007 0.000031 0.000048
Endoplasmic Reticulum 0.564441 0.375212 0.231468 0.501039 0.520824 0.274287 0.513226 0.580679 0.533370 0.308141 0.481878 0.574366
Endosome 0.024299 0.014311 0.018028 0.018745 0.012467 0.008012 0.009130 0.022108 0.015870 0.010125 0.010120 0.021842
Golgi 0.006984 0.000855 0.000888 0.001982 0.001238 0.000332 0.000137 0.004987 0.002891 0.000508 0.000220 0.004749
Lipid Particles 0.056239 0.003453 0.003484 0.006467 0.005068 0.002215 0.000855 0.008886 0.019786 0.002630 0.001147 0.008694
Mitochondria 0.002074 0.003488 0.000601 0.000289 0.001963 0.003424 0.001087 0.002761 0.001995 0.003445 0.001033 0.002565
Mitotic Spindle 0.009035 0.001770 0.003186 0.002144 0.000254 0.001393 0.001925 0.011184 0.002780 0.001519 0.002065 0.010467
None 0.000005 0.001925 0.000164 0.000052 0.000397 0.002679 0.000318 0.001097 0.000284 0.002426 0.000300 0.001014
Nuclear Periphery 0.073144 0.360379 0.462573 0.155302 0.243217 0.544022 0.329962 0.099541 0.194300 0.482423 0.344716 0.103961
Nuclear Periphery Foci 0.129896 0.201233 0.255152 0.249245 0.181637 0.125542 0.111891 0.198013 0.166755 0.150931 0.127830 0.202074
Nucleolus 0.000049 0.001015 0.000208 0.000129 0.000153 0.000787 0.000125 0.000240 0.000123 0.000864 0.000134 0.000231
Nucleus 0.000889 0.004188 0.000788 0.002325 0.006062 0.011905 0.008970 0.004919 0.004574 0.009317 0.008060 0.004713
Peroxisomes 0.000357 0.000291 0.000024 0.000092 0.000295 0.000301 0.000071 0.000202 0.000312 0.000298 0.000065 0.000193
Vacuole 0.080806 0.010878 0.011489 0.032921 0.006615 0.010359 0.007457 0.022692 0.027954 0.010533 0.007906 0.023503
Vacuole Periphery 0.006252 0.004981 0.002390 0.002424 0.003592 0.003757 0.000843 0.003811 0.004357 0.004168 0.001015 0.003701

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.84 1.50 1.73 -0.55 0.29 0.07 0.77 -2.10 -2.18 -2.36 1.79 1.91 -0.27 -2.14 -2.26
Bud Neck 3.25 -0.39 -0.55 -1.85 -0.31 0.79 0.32 -2.58 -5.75 -4.85 1.87 0.83 -2.46 -5.97 -4.53
Bud Site 2.32 2.59 1.73 -1.01 -1.56 1.23 1.07 -1.44 -4.64 -4.38 2.30 2.43 -0.45 -4.36 -4.54
Cell Periphery 1.55 2.71 2.09 0.40 -1.02 0.67 1.03 -1.02 -2.33 -2.74 1.47 2.97 1.19 -0.50 -2.83
Cytoplasm 1.15 3.10 -0.09 -0.81 -1.94 0.08 -0.88 -3.91 -7.24 -5.57 0.51 -0.19 -4.03 -7.14 -6.03
Cytoplasmic Foci 2.99 3.32 2.52 -0.55 -1.27 1.08 1.61 -1.31 -5.01 -6.45 2.88 3.53 0.79 -4.78 -6.56
Eisosomes 2.25 -0.07 1.52 -1.91 0.93 1.38 1.55 -1.51 -2.01 -2.07 2.41 1.28 0.72 -2.23 -0.70
Endoplasmic Reticulum 4.41 7.62 1.15 -3.45 -7.16 10.88 0.37 -2.16 -25.00 -4.78 11.18 2.38 -1.71 -23.43 -7.09
Endosome 1.74 0.99 0.83 -1.64 -0.24 2.29 1.67 -4.57 -11.05 -10.58 2.66 2.59 -2.72 -10.04 -9.99
Golgi 1.73 1.72 1.27 -0.77 -0.75 1.13 1.37 -2.78 -4.46 -4.67 2.03 2.29 -1.23 -4.38 -4.70
Lipid Particles 3.47 3.46 3.23 -1.63 -1.35 2.08 3.13 -2.54 -6.39 -7.86 3.67 3.96 2.29 -6.29 -7.84
Mitochondria -0.74 0.74 0.91 4.32 3.24 -2.87 2.36 -0.93 0.76 -1.99 -2.17 1.49 -0.64 1.07 -1.98
Mitotic Spindle 1.08 0.81 1.02 -0.33 0.30 -2.24 -2.56 -5.74 -4.92 -4.52 0.58 0.25 -3.13 -4.93 -4.41
None -2.37 -1.62 -2.52 2.31 1.07 -3.51 0.45 -2.08 2.27 -2.56 -4.18 -0.20 -2.47 2.48 -2.55
Nuclear Periphery -16.58 -17.33 -3.42 9.91 12.20 -16.30 -4.70 8.29 51.58 28.42 -19.48 -10.04 6.40 50.76 31.16
Nuclear Periphery Foci -3.42 -5.41 -3.83 -1.92 0.29 4.38 5.42 -1.12 -9.62 -11.33 1.43 3.49 -2.79 -7.06 -10.04
Nucleolus -2.75 -1.99 -1.16 2.44 0.62 -4.35 0.21 -2.00 3.19 -2.09 -4.93 -0.61 -2.49 3.63 -1.99
Nucleus -3.32 0.61 -1.45 1.01 -1.62 -1.49 -0.86 0.06 3.91 2.55 -1.67 -1.34 -0.34 3.18 2.28
Peroxisomes 0.46 2.61 1.78 2.28 -1.28 -0.12 2.38 0.86 0.76 -2.08 0.08 3.04 1.36 1.12 -2.26
Vacuole 4.57 4.53 2.27 -2.61 -2.55 -1.82 -0.59 -6.31 -6.10 -7.47 3.53 3.97 0.60 -6.70 -7.82
Vacuole Periphery 0.26 0.91 0.87 2.19 -0.07 -0.17 3.11 -0.16 -0.02 -4.23 0.10 2.50 0.43 0.72 -4.13
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
Localization
Cell Percentages ER (47%), nuclear periphery (1%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Alg9

Alg9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available