Standard name
Human Ortholog
Description Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0.08 0.06 0.14 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0.08 0.11 0.05 0.16 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0.09 0.08 0.09 0.09 0.1 – 0.09 0.12 0.1 0 0.05 0.08 0.24 0.25 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0.16 0.28 0.26 0.11 0.12 0.18 0.19 0.25 – 0.2 0.16 0.23 0.24 0.43 0.36 0 0.05 0.07 0.37 0.41 0.66 0.71 0.79 0.78
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.23 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.12 0.06 0.18 0.17 0.21 – 0.36 0.43 0.39 0 0 0 0.07 0.12 0.14 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0.65 0.44 0.58 0.56 0.41 0.31 0.28 0.22 – 0.17 0.16 0.21 0.46 0.34 0.17 0.28 0.27 0.2 0.53 0.43 0.26 0.19 0.11 0.07
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.51 0.47 0.26 0.28 0.46 0.4 0.39 0.39 – 0.33 0.27 0.23 0.46 0.36 0.48 0.26 0.36 0.14 0 0.09 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 3 4 1 5 15 – 8 6 18 7 18 21 6 5 6 0 0 2 4 7 5
Bud 0 0 0 1 1 3 1 3 – 8 4 9 1 3 0 1 0 0 2 3 0 4 3 6
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 1 0 0 – 2 1 1 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 4 4 10 1 3 4 – 12 12 20 14 36 65 8 4 7 0 0 0 0 0 0
Cytoplasm 4 4 20 28 28 25 30 44 – 35 40 36 12 45 70 17 21 8 0 0 0 0 0 3
Endoplasmic Reticulum 23 25 63 33 42 47 63 115 – 77 56 84 147 357 314 2 4 3 62 54 176 276 396 465
Endosome 0 0 3 2 1 2 6 2 – 3 2 4 0 2 4 0 0 0 0 0 1 2 4 4
Golgi 0 0 1 3 7 1 0 3 – 2 0 0 10 17 8 0 5 10 1 0 1 3 3 5
Mitochondria 2 4 18 37 21 47 56 95 – 137 149 142 1 0 1 5 10 6 3 1 5 10 12 9
Nucleus 2 0 1 6 5 2 3 11 – 9 11 5 13 18 13 4 1 1 1 0 0 0 0 1
Nuclear Periphery 95 40 140 170 146 83 94 101 – 65 54 77 283 283 144 20 23 9 90 56 67 73 54 44
Nucleolus 1 0 2 1 1 0 2 4 – 3 0 2 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 – 0 0 0 1 1 1 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 75 42 62 84 161 106 129 176 – 125 92 82 280 300 415 19 30 6 5 11 6 8 18 42
Unique Cell Count 147 90 243 304 353 266 332 455 384 343 364 611 829 861 72 84 44 171 133 266 388 505 594
Labelled Cell Count 204 115 314 372 427 319 393 574 486 427 480 770 1080 1058 82 103 56 171 133 266 388 505 594


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 11.8 10.6 11.3 11.7 10.5 10.2 9.9 – 8.9 9.0 9.2 13.4 12.3 13.4 10.1 11.3 15.2 13.8 15.2 15.6
Std Deviation (1e-4) 2.3 2.2 2.2 2.4 2.6 2.3 2.2 2.1 – 1.9 2.0 2.1 2.2 2.0 2.3 2.4 2.7 3.3 2.6 2.9 2.7
Intensity Change (Log2) – – – 0.1 0.14 -0.0 -0.04 -0.09 – -0.25 -0.24 -0.2 0.34 0.21 0.35 -0.06 0.09 0.52 0.39 0.52 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.9 – 0 0 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.9 0 0 0 – 1.1 1.4 2.4 0.6 2.0 3.4 0 0 0
Cytoplasm 0.4 -0.1 0.5 0.3 0.6 – 0.4 1.3 0.7 -4.4 -1.6 -0.1 3.6 4.0 0
Endoplasmic Reticulum -4.6 -4.4 -2.3 -2.0 -0.2 – -1.7 -2.8 -0.8 -0.6 4.8 3.1 -4.3 -4.1 -2.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 -0.7 3.5 3.3 4.6 – 8.0 9.5 8.7 -6.5 0 0 -0.1 1.3 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.4 -3.9 -6.0 -7.1 -9.4 – -10.6 -10.6 -9.2 -3.0 -6.6 -12.9 -4.4 -4.8 -4.5
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0.6 5.0 3.4 3.4 3.5 – 1.9 0.4 -0.8 5.5 3.1 6.3 0.1 1.8 -1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.5379 14.0675 13.8725 12.8501 11.8643 13.2849 16.6412 15.3933 14.0771 14.2509 14.6686 14.0952 16.3561 14.9628 15.0644 14.6327 13.7943 14.173
Actin 0.0131 0.0001 0.0092 0.0178 0.0022 0.001 0.0197 0 0.0051 0.0002 0.0166 0.0019 0.0012 0.0011 0.004 0.0005 0.0036 0.0003
Bud 0.0002 0 0.0001 0.0003 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0
Bud Neck 0.0053 0.0001 0.0006 0.0014 0.0007 0.0001 0.0002 0 0.0002 0.0001 0.0005 0 0 0.0001 0.0001 0.0001 0.0011 0
Bud Periphery 0.0003 0 0.0002 0.0007 0.0007 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0.0005 0.0003 0.0004 0.0002 0
Bud Site 0.0029 0 0.0008 0.0015 0.0012 0 0.0006 0 0.0005 0 0.0005 0 0.0001 0.0001 0.0001 0 0.0011 0
Cell Periphery 0.0003 0.0003 0.0016 0.0006 0.0006 0 0.0004 0.0002 0.0004 0.0002 0.0006 0.0001 0.0002 0.0076 0.0007 0.0003 0.0001 0
Cytoplasm 0.022 0.0036 0.0015 0.0063 0.0013 0.0019 0.0155 0.0001 0.0031 0.0003 0.0091 0.0001 0.0003 0.0079 0.0002 0.0002 0.0054 0.0001
Cytoplasmic Foci 0.0126 0.0001 0.0003 0.0071 0.003 0.0011 0.0034 0 0.0159 0 0.0304 0.0012 0.0035 0.0002 0.001 0 0.0123 0.0032
Eisosomes 0.0003 0 0.0006 0.0001 0.0019 0 0.0001 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0964 0.3155 0.2833 0.3306 0.2253 0.073 0.1152 0.3835 0.2995 0.3919 0.3424 0.125 0.0789 0.3235 0.3927 0.3985 0.204 0.1037
Endosome 0.0221 0.0011 0.0015 0.0081 0.0069 0.0048 0.0023 0.0002 0.0139 0.0007 0.0056 0.0016 0.0075 0.0025 0.001 0.0011 0.0207 0.0003
Golgi 0.0038 0.0001 0.0006 0.0026 0.003 0.0004 0.0043 0 0.0074 0 0.0021 0.001 0.0055 0.0001 0.0013 0.0001 0.0093 0.0002
Lipid Particles 0.0096 0.0009 0.003 0.0098 0.015 0.0014 0.0054 0.0001 0.0069 0.0001 0.0019 0.0013 0.0092 0.0004 0.002 0.0001 0.0212 0.0005
Mitochondria 0.002 0.0002 0.0005 0.0014 0.0347 0.0002 0.0012 0.0002 0.0008 0.0008 0.0004 0.0003 0.0025 0.0006 0.0043 0.0009 0.0025 0.0001
None 0.0051 0 0.0055 0.0011 0.0526 0.0004 0.0037 0 0.0016 0 0.0018 0.0001 0.0001 0.0002 0.0001 0.0001 0.001 0
Nuclear Periphery 0.7462 0.6131 0.6137 0.5596 0.5577 0.8636 0.7693 0.5564 0.5703 0.5498 0.527 0.8238 0.8298 0.5755 0.5258 0.5463 0.6414 0.848
Nucleolus 0.0008 0.0004 0.0008 0.0001 0.0036 0.0001 0 0 0.0002 0 0.0001 0 0.0001 0.0001 0 0 0.0007 0
Nucleus 0.0123 0.0291 0.0408 0.0223 0.029 0.0231 0.0047 0.0019 0.011 0.004 0.0019 0.0016 0.0075 0.0076 0.004 0.0055 0.0078 0.0033
Peroxisomes 0.0018 0 0.0001 0.0005 0.0159 0.001 0.0026 0 0.001 0 0.0045 0.0009 0.0004 0 0.0048 0 0.0114 0.0028
Punctate Nuclear 0.0145 0.0004 0.0018 0.0014 0.0011 0.004 0.0003 0 0.0027 0 0.0236 0.0004 0.0002 0.0004 0.0001 0 0.0132 0.0045
Vacuole 0.0045 0.0098 0.0092 0.0041 0.0108 0.0027 0.0034 0.0047 0.0071 0.0053 0.0037 0.0018 0.0034 0.0201 0.0064 0.0056 0.0062 0.0015
Vacuole Periphery 0.0239 0.0251 0.0242 0.0224 0.0327 0.0211 0.0474 0.0525 0.0524 0.0465 0.0268 0.039 0.0494 0.0513 0.0511 0.0402 0.0365 0.0313

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.8592 57.3612 43.0033 53.5475 51.4609 40.9911 47.6838 47.0139 38.2332 58.4515
Translational Efficiency 0.7032 0.6372 0.7373 0.4651 0.5448 1.0422 0.7234 0.6317 0.7339 0.5276

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
Localization
Cell Percentages ER (47%), nuclear periphery (1%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Alg9

Alg9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available