Standard name
Human Ortholog
Description Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.61 0.49 0.5 0.83 0.89 0.73 0.63 0.64 0.68 0.61 0.62 0.68 0.51 0.87 0.96 0.43 0.45 0.51 0.46 0.28 0.42 0.64 0.67 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.1 0.16 0 0.05 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.15 0.15 0.29 0.56 0.5 0.55 0.55 0.6 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0.16 0.07 0 0 0.06 0 0 0 0 0 0 0.11 0 0 0.1 0.05 0.09 0.27 0.41 0.23 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0 0 0
Vac/Vac Membrane 0.49 0.51 0.36 0.07 0 0 0 0 0 0 0 0 0.43 0.11 0 0.5 0.55 0.44 0.1 0.12 0.12 0.17 0.12 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 3 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 2
Bud 0 0 1 0 2 10 7 10 10 8 18 11 2 1 1 0 0 0 4 2 2 4 3 8
Bud Neck 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1 2 1 3
Bud Site 0 0 0 0 0 0 3 3 2 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 2 2 2 0 0 3 2 2 4 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 161 42 91 204 408 449 487 473 374 361 394 448 68 219 326 80 107 127 137 34 94 113 162 193
Endoplasmic Reticulum 3 0 2 0 1 12 3 0 0 5 0 1 0 11 1 6 7 7 1 0 2 3 3 7
Endosome 8 0 9 13 7 2 1 1 0 2 2 0 5 3 9 19 38 10 15 6 7 1 10 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 1 4
Mitochondria 3 0 28 37 67 176 432 374 300 330 383 374 0 0 0 4 6 1 3 0 4 2 1 7
Nucleus 25 14 12 1 1 34 25 11 11 9 12 12 15 2 0 18 12 22 80 50 52 10 10 6
Nuclear Periphery 0 0 0 0 0 0 5 5 1 3 1 0 0 1 0 0 0 0 2 0 1 0 0 0
Nucleolus 0 0 1 2 0 1 1 1 0 0 0 1 0 0 0 1 1 0 5 3 6 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 2 1 2 0 4 2 5 0 0 1 1 2 0 8 7 19 5 9 7
Vac/Vac Membrane 129 44 66 16 11 7 32 22 14 18 13 2 58 28 5 94 131 111 29 15 26 30 28 30
Unique Cell Count 265 86 181 246 459 615 774 743 550 596 634 663 134 252 339 188 239 251 298 124 226 178 241 294
Labelled Cell Count 330 100 210 276 499 699 997 903 715 743 829 860 149 265 344 224 305 279 298 124 226 178 241 294


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.6 4.6 4.5 4.5 3.8 3.2 3.4 3.0 3.0 2.9 3.0 6.9 6.6 6.8 7.6 7.7 7.8 7.6 7.7 8.1
Std Deviation (1e-4) 0.7 0.9 0.6 1.2 1.2 0.6 0.9 1.0 0.7 0.6 0.6 0.7 1.3 1.1 1.1 1.1 1.2 1.1 2.2 2.0 2.3
Intensity Change (Log2) – – – -0.02 -0.03 -0.27 -0.53 -0.44 -0.63 -0.61 -0.68 -0.63 0.58 0.52 0.58 0.72 0.75 0.76 0.72 0.76 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.2 10.6 5.8 3.1 3.3 4.3 2.5 2.9 4.3 0.1 8.3 12.5 -1.5 -1.1 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 -0.5 -2.4 -1.4 1.9 3.5 -0.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.8 -6.5 -7.4 -4.6 -4.8 -6.2
Nucleus -3.7 0 -0.6 -2.1 0 -3.1 0 -3.3 -3.5 1.4 -3.4 0 1.0 -0.7 0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -7.8 -11.9 -14.5 -12.9 -13.8 -12.7 -12.7 -13.8 -15.8 1.2 -6.3 -11.1 2.6 3.7 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0005 0.0003 0.0007 0.0004 0.0004 0.0004 0.0133 0.0003 0.0003 0.0008 0.0098 0.0044 – – – – – –
Bud 0.0017 0.0002 0.0021 0.0003 0.0002 0.001 0.0005 0.0004 0.0004 0.0045 0.0001 0.0003 – – – – – –
Bud Neck 0.0009 0.0018 0.0021 0.001 0.0014 0.004 0.0021 0.0035 0.0025 0.0023 0.0012 0.0052 – – – – – –
Bud Periphery 0.0004 0.0001 0.0008 0.0003 0.0001 0.0007 0.0004 0.0002 0.0002 0.0026 0.0001 0.0003 – – – – – –
Bud Site 0.0016 0.0061 0.0035 0.001 0.0003 0.0006 0.0022 0.0057 0.0033 0.0018 0.0003 0.0011 – – – – – –
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 – – – – – –
Cytoplasm 0.5864 0.4987 0.5604 0.4772 0.4339 0.569 0.5717 0.5419 0.5648 0.4929 0.4836 0.5312 – – – – – –
Cytoplasmic Foci 0.0301 0.0206 0.0214 0.0112 0.0169 0.0193 0.0291 0.0257 0.022 0.0283 0.028 0.017 – – – – – –
Eisosomes 0 0 0.0001 0 0 0 0.0002 0 0 0 0.0001 0.0001 – – – – – –
Endoplasmic Reticulum 0.0113 0.0052 0.0069 0.0038 0.0075 0.0045 0.0122 0.0103 0.0082 0.0089 0.0078 0.0074 – – – – – –
Endosome 0.0279 0.023 0.0228 0.0119 0.0129 0.0157 0.0351 0.0342 0.0217 0.0201 0.0208 0.0165 – – – – – –
Golgi 0.0006 0.0005 0.0005 0.0003 0.0003 0.0003 0.0026 0.001 0.0008 0.0005 0.0029 0.0016 – – – – – –
Lipid Particles 0.0011 0.0007 0.0009 0.0004 0.0002 0.0003 0.0078 0.0008 0.0012 0.0004 0.0063 0.004 – – – – – –
Mitochondria 0.003 0.0006 0.0009 0.0023 0.001 0.0008 0.0019 0.0008 0.0006 0.0008 0.0014 0.0024 – – – – – –
None 0.1729 0.1982 0.2096 0.2313 0.2052 0.1413 0.1487 0.1285 0.1614 0.2121 0.1724 0.123 – – – – – –
Nuclear Periphery 0.0155 0.0104 0.0118 0.0121 0.0309 0.0099 0.0178 0.0089 0.0104 0.0111 0.0179 0.0105 – – – – – –
Nucleolus 0.0017 0.0102 0.0024 0.0019 0.002 0.0031 0.0023 0.0143 0.005 0.0009 0.0009 0.0053 – – – – – –
Nucleus 0.1149 0.1886 0.1339 0.2114 0.2153 0.2005 0.1189 0.1937 0.1685 0.1979 0.2167 0.2429 – – – – – –
Peroxisomes 0.0009 0.0003 0.0005 0.0004 0.0004 0.0003 0.0061 0.0004 0.0003 0.0005 0.005 0.005 – – – – – –
Punctate Nuclear 0.0177 0.0257 0.0131 0.0273 0.0656 0.0237 0.0163 0.0142 0.0192 0.0084 0.0208 0.0145 – – – – – –
Vacuole 0.0096 0.008 0.005 0.0043 0.0045 0.0041 0.0095 0.0138 0.0085 0.0045 0.0031 0.0068 – – – – – –
Vacuole Periphery 0.0012 0.0007 0.0006 0.0009 0.0009 0.0005 0.0011 0.0012 0.0007 0.0007 0.0007 0.0006 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5645 11.9872 13.6602 15.145 10.2453 13.8806 15.1503 17.3833 20.7404 18.3066
Translational Efficiency 1.2887 1.1945 1.065 1.1587 1.5796 1.5673 1.2781 0.9348 0.9032 1.006

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
148 1615 234 122 1678 1713 200 1622 1826 3328 434 1744

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 827.07 840.66 1091.24 1192.99 773.02 801.48 1156.25 1082.33 777.40 820.49 1121.20 1090.07
Standard Deviation 97.23 113.58 136.20 157.47 99.43 104.93 122.45 154.83 100.35 110.96 134.02 157.57
Intensity Change Log 2 — 0.023513 0.399887 0.528501 — 0.052161 0.580876 0.485563 — 0.037424 0.490162 0.507916

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000435 0.000207 0.000895 0.000692 0.000112 0.000226 0.001314 0.002054 0.000138 0.000217 0.001088 0.001959
Bud Neck 0.001879 0.002667 0.004653 0.006453 0.002028 0.007149 0.005157 0.022048 0.002016 0.004974 0.004885 0.020957
Bud Site 0.004237 0.004235 0.003120 0.006155 0.001458 0.005616 0.004230 0.015741 0.001683 0.004946 0.003631 0.015071
Cell Periphery 0.000329 0.000115 0.000185 0.000120 0.000092 0.000116 0.000221 0.000195 0.000111 0.000115 0.000201 0.000189
Cytoplasm 0.444119* 0.486693* 0.199585* 0.216640* 0.601614* 0.501200* 0.140342* 0.264621* 0.588849* 0.494160* 0.172284* 0.261264*
Cytoplasmic Foci 0.139309 0.041491 0.003090 0.009822 0.098070 0.121127 0.003327 0.007271 0.101412 0.082482 0.003199 0.007450
Eisosomes 0.000126 0.000025 0.000042 0.000045 0.000037 0.000058 0.000069 0.000028 0.000044 0.000042 0.000054 0.000029
Endoplasmic Reticulum 0.005868 0.002974 0.012017 0.005847 0.002300 0.003246 0.021581 0.006870 0.002589 0.003114 0.016424 0.006799
Endosome 0.013473 0.002870 0.003193 0.006886 0.004960 0.008721 0.003943 0.008336 0.005650 0.005882 0.003539 0.008235
Golgi 0.001538 0.000569 0.000148 0.000274 0.000465 0.001962 0.000150 0.003646 0.000552 0.001286 0.000149 0.003410
Lipid Particles 0.012710 0.001849 0.001633 0.003833 0.003876 0.007117 0.002410 0.000996 0.004592 0.004561 0.001991 0.001195
Mitochondria 0.010713 0.001663 0.000705 0.000393 0.001671 0.005204 0.001661 0.010300 0.002404 0.003486 0.001145 0.009607
Mitotic Spindle 0.002377 0.003837 0.003823 0.294661* 0.000985 0.005009 0.001584 0.087660 0.001098 0.004440 0.002791 0.102140*
None 0.005900 0.007163 0.007892 0.008783 0.006740 0.003741 0.003694 0.011229 0.006672 0.005402 0.005957 0.011058
Nuclear Periphery 0.001311 0.001477 0.002529 0.002808 0.000957 0.001677 0.004442 0.006084 0.000986 0.001580 0.003410 0.005854
Nuclear Periphery Foci 0.009697 0.002909 0.004538 0.021152 0.002741 0.002785 0.006626 0.004558 0.003305 0.002845 0.005500 0.005719
Nucleolus 0.003603 0.002276 0.000623 0.003112 0.001281 0.001951 0.001441 0.012594 0.001469 0.002109 0.001000 0.011931
Nucleus 0.280044* 0.402979* 0.673138* 0.174866* 0.239098* 0.277464* 0.674419* 0.477827* 0.242416* 0.338373* 0.673729* 0.456633*
Peroxisomes 0.002877 0.000756 0.000200 0.000775 0.000974 0.003794 0.000383 0.001165 0.001129 0.002320 0.000284 0.001138
Vacuole 0.057581 0.032653 0.077785 0.236354* 0.030116 0.040826 0.122692 0.052053 0.032342 0.036860 0.098479 0.064945
Vacuole Periphery 0.001874 0.000591 0.000208 0.000328 0.000424 0.001010 0.000315 0.004725 0.000542 0.000806 0.000257 0.004418

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.73 -5.87 -3.25 -6.20 2.37 -3.35 -9.12 -3.26 -2.87 1.29 -3.14 -13.38 -3.36 -3.06 1.18
Bud Neck -1.80 -4.53 -6.17 -5.81 -2.60 -6.52 -4.94 -17.77 -13.62 -8.96 -6.58 -6.72 -18.24 -15.96 -11.21
Bud Site -0.17 -1.06 -4.62 -4.72 -4.19 -4.61 -4.69 -9.41 -4.60 -3.67 -5.17 -6.31 -9.96 -5.97 -5.04
Cell Periphery 6.54 2.60 4.50 -2.82 2.52 -2.51 -6.50 -2.47 -0.84 3.96 -0.30 -8.13 -1.41 -1.13 5.43
Cytoplasm -1.61 11.02 9.67 16.48 -0.26 10.96 38.52 48.49 35.07 -6.52 12.60 43.16 48.74 41.30 -5.52
Cytoplasmic Foci 8.26 11.72 11.13 12.50 -3.78 -4.37 26.92 26.86 29.63 -1.48 4.70 29.52 28.84 32.00 -2.18
Eisosomes 7.82 4.85 4.94 -5.71 0.30 -6.62 -6.48 -0.08 7.06 6.54 1.03 -5.64 2.37 1.65 7.37
Endoplasmic Reticulum 2.98 -6.29 -0.09 -3.68 6.57 -2.19 -11.15 -13.39 -8.18 8.04 -2.24 -14.81 -12.93 -10.49 9.95
Endosome 5.91 4.58 2.31 -4.37 -2.55 -4.65 0.15 0.53 5.21 0.30 -0.11 1.37 1.28 1.54 -0.13
Golgi 1.47 1.92 1.51 0.05 -3.29 -4.22 2.61 -1.77 1.41 -2.38 -3.18 3.36 -1.49 0.27 -2.45
Lipid Particles 5.08 4.84 3.73 -4.48 -3.50 -4.39 2.51 5.84 9.30 4.83 0.05 4.56 6.90 8.84 4.65
Mitochondria 1.41 1.53 1.54 1.59 0.79 -3.11 0.18 -2.94 0.46 -3.55 -1.16 1.48 -1.88 -0.99 -4.20
Mitotic Spindle -0.80 -1.32 -5.45 -5.39 -5.29 -3.46 -2.05 -14.54 -13.40 -7.70 -3.63 -2.69 -15.46 -14.64 -11.84
None -0.72 0.60 -1.27 -0.97 -1.84 4.12 4.18 -3.48 -8.52 -8.01 2.01 3.02 -3.69 -6.62 -6.76
Nuclear Periphery -1.37 -6.98 -5.97 -5.60 -1.50 -3.65 -8.94 -18.76 -16.14 -4.08 -4.83 -12.23 -19.25 -17.22 -7.46
Nuclear Periphery Foci 3.80 1.13 -2.97 -4.90 -3.66 0.09 -3.16 -6.07 -5.53 1.02 0.86 -4.07 -6.56 -6.81 -0.17
Nucleolus 0.91 2.46 -0.75 -1.74 -3.25 -1.51 -1.56 -10.49 -10.15 -6.43 -1.71 -1.09 -10.49 -10.00 -8.98
Nucleus -5.53 -12.37 2.30 8.42 14.60 -4.80 -18.22 -28.74 -23.48 5.01 -14.02 -26.39 -26.72 -16.48 8.75
Peroxisomes 2.42 2.96 1.98 -1.20 -3.05 -5.89 3.26 1.78 7.19 -2.04 -3.77 4.40 2.40 6.68 -3.00
Vacuole 2.99 -5.52 -10.44 -11.45 -7.24 -3.98 -9.26 -14.93 -12.38 4.54 -2.54 -12.04 -16.92 -16.01 3.21
Vacuole Periphery 1.83 2.27 2.17 1.12 -1.03 -5.71 1.73 -2.69 -1.25 -2.92 -2.21 3.07 -2.36 -1.67 -2.97
Standard name
Human Ortholog
Description Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sqs1

Sqs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sqs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available