Standard name
Human Ortholog
Description Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; may control nonselective autophagy and mitophagy through trafficking of Atg9p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.99 0.99 0.97 0.98 0.92 0.94 0.86 – 0.79 0.68 1.0 0.97 0.96 – 0.94 0.96 0.74 0.79 0.6 0.84 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.06 0 0.09 0.16 0.16 0.3 – 0.39 0.43 0 0 0 – 0 0 0.15 0.09 0.22 0.06 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 2 3 5 1 3 2
Bud 1 1 1 2 1 1 10 2 4 – 7 5 0 0 11 – 0 1 0 1 0 0 0 3
Bud Neck 4 1 1 3 11 4 9 5 3 – 7 5 0 0 0 – 4 6 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 1 1 1 – 0 3 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 3 1 3 3 1 3 2 – 0 2 0 1 0 – 3 3 2 2 4 0 1 1
Cytoplasm 212 250 195 246 311 384 392 461 232 – 215 240 150 296 295 – 244 388 166 200 103 135 234 245
Endoplasmic Reticulum 1 1 0 0 1 0 0 0 0 – 0 1 0 10 9 – 9 28 1 0 2 0 2 5
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 5 5 3 1 0 0 0 1
Golgi 1 1 0 0 1 0 0 0 0 – 0 0 0 0 0 – 1 0 1 0 1 1 0 0
Mitochondria 3 15 4 14 9 36 68 76 82 – 105 153 4 1 5 – 5 9 34 24 38 9 9 15
Nucleus 1 0 0 1 0 0 1 3 0 – 0 2 0 1 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 – 0 3 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 2 2 – 1 1 0 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 – 1 0 0 0 0 0 0 0
SpindlePole 1 1 0 1 0 0 6 8 4 – 5 12 0 0 1 – 3 2 3 2 1 1 1 4
Vac/Vac Membrane 1 2 0 0 0 0 2 0 1 – 0 0 0 0 0 – 4 7 1 7 2 0 5 6
Unique Cell Count 218 260 196 249 322 393 425 489 269 271 353 150 306 306 260 404 225 254 172 161 268 292
Labelled Cell Count 226 273 204 268 337 429 491 561 331 340 428 154 309 321 279 450 225 254 172 161 268 292


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.1 2.9 2.6 2.9 2.6 2.5 2.7 2.2 – 2.3 2.1 3.9 4.2 3.7 4.7 5.1 5.1 3.9 3.4 4.4
Std Deviation (1e-4) 0.7 0.6 0.9 0.9 0.8 0.8 1.2 1.1 1.1 – 0.9 1.1 1.3 1.8 1.7 1.7 2.1 1.6 1.3 1.1 2.0
Intensity Change (Log2) – – – -0.12 0.01 -0.13 -0.17 -0.1 -0.35 – -0.34 -0.43 0.44 0.56 0.37 0.73 0.84 0.83 0.44 0.24 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2077 -0.462 -0.4974 -0.6731 -0.9278 -0.4857 1.3716 0.7348 0.5687 0.5939 0.8906 0.5678 -0.2438 0.2158 -0.2484 -0.0151 -0.2876 0.0507
Actin 0.062 0.0008 0.0046 0.0027 0.0009 0.0016 0.0832 0.0002 0.0148 0.0125 0.0297 0.0101 0.0107 0.0001 0.0001 0.0001 0 0.0001
Bud 0.0011 0.0008 0.0008 0.0084 0 0.0006 0.0037 0.0001 0.0022 0.0002 0.0016 0.0011 0.001 0.0001 0 0 0 0
Bud Neck 0.0115 0.0002 0.0032 0.004 0 0.0038 0.0091 0 0.0003 0.0002 0.0011 0.0015 0.0008 0.0002 0 0 0 0
Bud Periphery 0.0021 0.0013 0.0018 0.0255 0.0001 0.0023 0.0081 0.0002 0.0097 0.0003 0.0043 0.0046 0.0034 0.0002 0.0001 0 0 0
Bud Site 0.0124 0.003 0.0123 0.0033 0 0.0044 0.0322 0.0002 0.0018 0.0007 0.004 0.0006 0.0008 0.0003 0 0 0 0
Cell Periphery 0.0005 0.0002 0.0007 0.0009 0 0.0007 0.0012 0.0001 0.0003 0.0001 0.0003 0.0001 0.0009 0.0005 0.0001 0 0 0
Cytoplasm 0.0125 0.038 0.0189 0.0361 0.0056 0.0425 0.0355 0.0584 0.0281 0.0284 0.0721 0.0847 0.025 0.016 0.0147 0.0103 0.0346 0.0199
Cytoplasmic Foci 0.0323 0.0368 0.0072 0.0165 0.0012 0.0174 0.0474 0.0054 0.0108 0.0054 0.007 0.0137 0.0034 0.0043 0.0019 0.0022 0.007 0.0033
Eisosomes 0.0007 0.0002 0.0003 0.0003 0.0004 0.0002 0.001 0.0001 0.0002 0.0002 0.0004 0.0002 0.0007 0.0003 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.003 0.0008 0.0005 0.0011 0 0.0002 0.0073 0.0005 0.0011 0.0019 0.0008 0.0016 0.0027 0.0004 0.0003 0.0002 0.0008 0.0003
Endosome 0.0415 0.003 0.0026 0.0016 0 0.0003 0.0401 0.0048 0.0123 0.0218 0.0273 0.0092 0.0054 0.0038 0.0005 0.0007 0.0132 0.0035
Golgi 0.0308 0.0005 0.0029 0.0005 0 0.0001 0.0142 0.0002 0.0039 0.0078 0.0106 0.0069 0.0005 0.0001 0 0 0.0001 0
Lipid Particles 0.0367 0.0017 0.0017 0.0012 0.0001 0.0007 0.0348 0.0005 0.0034 0.008 0.0247 0.0106 0.0028 0.0006 0.001 0.0004 0.0002 0.0003
Mitochondria 0.0165 0.0011 0.0052 0.0039 0.0001 0.0002 0.0166 0.0002 0.0192 0.0044 0.0297 0.0083 0.0007 0.0003 0.0001 0.0002 0.0001 0.0001
None 0.6882 0.9037 0.9301 0.8858 0.9911 0.9144 0.6037 0.923 0.8588 0.9014 0.7361 0.8195 0.9103 0.918 0.9794 0.9843 0.9402 0.9681
Nuclear Periphery 0.0052 0.0003 0.0003 0.0005 0 0.0002 0.0166 0.0003 0.0013 0.0028 0.0011 0.0069 0.0045 0.0002 0.0001 0.0002 0.0002 0.0001
Nucleolus 0.0017 0.0004 0.0024 0.0013 0 0.0003 0.0011 0.0002 0.0007 0.0001 0.0006 0.0012 0.0023 0.0006 0.0001 0.0001 0 0.0001
Nucleus 0.002 0.0008 0.0006 0.0011 0 0.0008 0.0021 0.0002 0.0016 0.0004 0.0008 0.0029 0.0023 0.0014 0.0001 0.0001 0.0001 0.0003
Peroxisomes 0.0293 0.0036 0.0013 0.0013 0.0002 0.0003 0.0138 0.0002 0.0142 0.001 0.0282 0.0036 0.0003 0.0001 0.0001 0.0001 0 0
Punctate Nuclear 0.006 0.0023 0.0014 0.0011 0.0001 0.0087 0.0193 0.0006 0.0018 0.0011 0.0011 0.0043 0.0107 0.0002 0.0003 0.0002 0.0001 0.0001
Vacuole 0.0028 0.0006 0.001 0.0024 0 0.0003 0.0063 0.0042 0.0125 0.0007 0.0113 0.006 0.009 0.051 0.0007 0.0007 0.0027 0.0033
Vacuole Periphery 0.0014 0.0001 0.0002 0.0005 0 0 0.0027 0.0003 0.001 0.0006 0.0073 0.0023 0.0017 0.0013 0 0.0001 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.1653 16.0236 16.692 25.8125 18.7709 20.6709 27.5736 26.1806 22.7768 28.1858
Translational Efficiency 0.8187 0.8527 0.8884 0.5358 0.8148 0.7103 0.5448 0.4771 0.6144 0.5484

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
163 1276 1440 1209 1572 1401 185 104 1735 2677 1625 1313

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 639.85 688.52 812.14 782.02 616.86 684.51 913.61 819.83 619.02 686.42 823.69 785.01
Standard Deviation 86.48 98.08 87.72 104.36 71.33 88.48 84.04 88.94 73.20 93.20 93.06 103.72
Intensity Change Log 2 — 0.105765 0.343995 0.289472 — 0.150129 0.566635 0.410382 — 0.127711 0.457572 0.350087

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000990 0.000191 0.000959 0.000085 0.000459 0.000342 0.000280 0.000083 0.000712 0.000208 0.000905
Bud Neck 0.038541 0.052679 0.002723 0.006179 0.010574 0.046461 0.001706 0.003868 0.013202 0.049425 0.002608 0.005996
Bud Site 0.005691 0.019123 0.002070 0.019014 0.002034 0.016971 0.001548 0.002857 0.002378 0.017996 0.002011 0.017734
Cell Periphery 0.000303 0.000175 0.000119 0.000172 0.000098 0.000444 0.000208 0.000152 0.000117 0.000316 0.000129 0.000170
Cytoplasm 0.337473* 0.302123* 0.195615* 0.466482* 0.503875* 0.323937* 0.155819* 0.393502* 0.488242* 0.313539* 0.191084* 0.460701*
Cytoplasmic Foci 0.284611* 0.302870* 0.005448 0.029090 0.188038* 0.260294* 0.012788 0.021517 0.197111* 0.280588* 0.006284 0.028490
Eisosomes 0.000332 0.000215 0.000032 0.000054 0.000111 0.000245 0.000105 0.000085 0.000132 0.000231 0.000040 0.000057
Endoplasmic Reticulum 0.001473 0.000697 0.003048 0.001450 0.000552 0.000829 0.005843 0.005118 0.000639 0.000766 0.003366 0.001740
Endosome 0.020154 0.036842 0.001527 0.012848 0.006243 0.032130 0.004319 0.002805 0.007550 0.034376 0.001845 0.012052
Golgi 0.001430 0.012051 0.000044 0.004427 0.001473 0.007818 0.000383 0.000051 0.001469 0.009836 0.000083 0.004080
Lipid Particles 0.020018 0.014228 0.001188 0.001703 0.008966 0.017302 0.003917 0.002330 0.010004 0.015837 0.001499 0.001753
Mitochondria 0.013686 0.026708 0.000469 0.003456 0.004761 0.023710 0.011523 0.000170 0.005599 0.025139 0.001728 0.003195
Mitotic Spindle 0.000545 0.005009 0.001807 0.056587 0.001409 0.010435 0.000254 0.000325 0.001328 0.007849 0.001631 0.052131
None 0.001152 0.002910 0.004002 0.007023 0.004251 0.003234 0.003064 0.004686 0.003960 0.003080 0.003895 0.006838
Nuclear Periphery 0.000604 0.000558 0.001978 0.001705 0.000686 0.001291 0.002212 0.001454 0.000678 0.000942 0.002005 0.001685
Nuclear Periphery Foci 0.000614 0.000317 0.000621 0.000921 0.000279 0.000454 0.001948 0.001599 0.000310 0.000389 0.000772 0.000975
Nucleolus 0.001690 0.002925 0.000586 0.001035 0.001417 0.002603 0.000786 0.000258 0.001443 0.002756 0.000609 0.000974
Nucleus 0.181333* 0.105858 0.755416* 0.261028* 0.230452* 0.144883* 0.687328* 0.359321* 0.225838* 0.126282 0.747664* 0.268814*
Peroxisomes 0.008920 0.037096 0.000553 0.003107 0.004052 0.022934 0.000346 0.000275 0.004509 0.029684 0.000530 0.002883
Vacuole 0.078622 0.072931 0.022442 0.116048 0.030049 0.080092 0.104649 0.199247 0.034613 0.076679 0.031801 0.122638
Vacuole Periphery 0.002747 0.003693 0.000119 0.006713 0.000594 0.003474 0.000912 0.000099 0.000797 0.003579 0.000209 0.006189

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.56 -6.13 -3.02 1.23 -1.71 -5.71 -8.66 -5.14 0.22 0.04 -3.60 -8.08 -3.12 0.96 -1.65
Bud Neck -1.51 4.03 3.74 14.36 -4.50 -12.58 6.71 1.62 12.06 -2.19 -15.01 7.72 5.35 20.36 -5.02
Bud Site -5.34 2.02 -2.52 3.17 -5.67 -8.20 -1.16 -4.50 5.77 -3.78 -11.64 -0.06 -5.95 3.71 -5.84
Cell Periphery 3.21 4.41 4.78 4.20 1.17 -3.04 -6.57 -3.81 2.39 1.81 -3.28 -1.95 -0.03 3.27 1.80
Cytoplasm 1.91 8.42 -0.23 -3.70 -16.19 18.71 25.04 9.28 2.48 -4.12 21.42 37.62 15.15 -2.44 -16.90
Cytoplasmic Foci -0.91 14.71 14.03 42.88 -8.94 -9.18 33.70 27.22 33.83 -2.19 -12.59 39.54 36.13 57.03 -8.96
Eisosomes 3.96 11.46 10.37 8.93 -8.90 -7.71 -2.40 -1.37 6.07 0.87 -7.28 10.54 7.84 13.73 -6.31
Endoplasmic Reticulum 1.16 -3.99 -1.86 -14.93 6.43 -2.30 -11.29 -6.18 -5.93 0.45 -1.21 -18.82 -14.34 -14.71 6.41
Endosome -4.39 5.82 4.55 14.20 -3.64 -13.90 1.65 3.84 15.83 2.18 -18.21 8.47 2.33 18.44 -3.37
Golgi -8.65 3.84 0.20 8.39 -2.56 -6.67 4.46 5.54 8.48 1.03 -10.90 6.11 0.35 9.70 -2.53
Lipid Particles 1.02 3.32 3.29 10.47 -1.42 -5.08 5.09 7.13 9.71 2.34 -4.47 9.39 9.43 14.87 0.16
Mitochondria -2.26 2.56 2.35 11.04 -3.64 -8.62 -0.06 6.24 11.38 1.02 -11.23 4.65 4.75 15.26 -0.76
Mitotic Spindle -3.90 -1.36 -8.04 -6.82 -7.74 -4.51 1.70 -0.78 3.17 -1.44 -5.39 -0.38 -7.88 -5.95 -7.79
None -2.70 -4.60 -6.46 -2.83 -2.95 1.32 3.42 1.27 0.20 -1.16 1.17 0.92 -1.94 -3.24 -3.16
Nuclear Periphery 0.28 -13.25 -15.70 -19.15 -6.01 -2.39 -10.20 -7.34 -4.04 0.51 -1.70 -15.09 -16.83 -13.19 -5.70
Nuclear Periphery Foci 0.94 -0.50 -0.71 -7.10 -0.58 -0.99 -6.00 -6.57 -5.55 -0.25 -0.71 -6.61 -8.53 -5.82 -0.18
Nucleolus -1.60 2.05 1.74 4.58 -1.86 -3.28 3.87 6.09 7.25 3.59 -4.06 4.73 3.78 7.07 -1.54
Nucleus 5.21 -37.78 -10.03 -25.58 40.29 11.95 -16.83 -3.27 -6.67 9.07 16.92 -63.84 -12.48 -25.44 40.39
Peroxisomes -8.36 3.63 3.31 14.46 -1.44 -11.48 9.96 10.09 14.15 0.87 -16.78 9.28 6.25 19.17 -1.45
Vacuole 0.29 4.32 -11.79 -18.22 -24.48 -13.77 -9.14 -11.52 -9.71 -5.13 -14.84 -4.94 -26.75 -20.38 -23.13
Vacuole Periphery -0.86 3.31 0.64 1.83 -3.03 -6.53 0.30 7.76 7.68 1.22 -8.42 6.20 -1.92 2.21 -2.96
Standard name
Human Ortholog
Description Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; may control nonselective autophagy and mitophagy through trafficking of Atg9p
Localization
Cell Percentages cytoplasm (15%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pik1

Pik1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pik1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available