Standard name | |
---|---|
Human Ortholog | |
Description | Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; functionally redundant with BNR1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 68 | 41 | 37 | 25 | 15 | 24 | 3 | 11 | 13 | 0 | 9 | 0 | 31 | 25 | 9 | – | – | – | 51 | 45 | 74 | 48 | 68 | 54 |
Bud | 17 | 16 | 72 | 46 | 50 | 52 | 31 | 82 | 64 | 27 | 42 | 17 | 30 | 15 | 22 | – | – | – | 43 | 61 | 49 | 67 | 82 | 67 |
Bud Neck | 0 | 0 | 26 | 3 | 26 | 14 | 18 | 18 | 11 | 4 | 4 | 8 | 0 | 0 | 0 | – | – | – | 52 | 75 | 92 | 64 | 35 | 24 |
Bud Site | 23 | 29 | 34 | 40 | 24 | 43 | 17 | 17 | 23 | 6 | 12 | 3 | 13 | 0 | 1 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 18 | 11 | 17 | 14 | 7 | 11 | 10 | 14 | 28 | 26 | 22 | 12 | 25 | 43 | 47 | – | – | – | 7 | 5 | 14 | 1 | 2 | 3 |
Cytoplasm | 154 | 202 | 340 | 277 | 292 | 436 | 447 | 568 | 443 | 412 | 312 | 301 | 244 | 385 | 458 | – | – | – | 113 | 141 | 196 | 33 | 76 | 99 |
Endoplasmic Reticulum | 13 | 10 | 2 | 0 | 1 | 4 | 1 | 3 | 4 | 0 | 4 | 0 | 12 | 7 | 4 | – | – | – | 10 | 7 | 9 | 3 | 8 | 8 |
Endosome | 3 | 12 | 6 | 3 | 1 | 1 | 0 | 11 | 0 | 0 | 1 | 0 | 4 | 2 | 0 | – | – | – | 13 | 12 | 17 | 6 | 11 | 7 |
Golgi | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 1 | 0 | 1 | 2 | 0 |
Mitochondria | 6 | 16 | 28 | 75 | 57 | 160 | 101 | 243 | 246 | 92 | 221 | 90 | 1 | 2 | 4 | – | – | – | 10 | 15 | 61 | 4 | 8 | 7 |
Nucleus | 0 | 1 | 2 | 0 | 2 | 3 | 4 | 4 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 4 | 0 | 1 | 1 | 2 | 9 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 15 | 24 | 20 | 11 | 1 | 11 | 0 | 6 | 8 | 1 | 2 | 0 | 13 | 2 | 1 | – | – | – | 1 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 28 | 15 | 62 | 39 | 41 | 31 | 30 | 54 | 28 | 13 | 12 | 6 | 4 | 2 | 0 | – | – | – | 4 | 3 | 17 | 3 | 7 | 3 |
Vac/Vac Membrane | 4 | 8 | 4 | 1 | 0 | 2 | 1 | 24 | 3 | 1 | 4 | 4 | 2 | 1 | 1 | – | – | – | 3 | 14 | 17 | 1 | 7 | 4 |
Unique Cell Count | 262 | 317 | 496 | 404 | 412 | 625 | 577 | 841 | 664 | 487 | 511 | 370 | 308 | 421 | 472 | 321 | 394 | 567 | 240 | 315 | 288 | |||
Labelled Cell Count | 352 | 386 | 654 | 534 | 519 | 793 | 666 | 1064 | 874 | 585 | 646 | 445 | 380 | 484 | 547 | 321 | 394 | 567 | 240 | 315 | 288 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 5.9 | 4.5 | 4.4 | 3.4 | 3.8 | 3.0 | 3.9 | 3.4 | 3.0 | 3.4 | 2.9 | 6.3 | 6.2 | 5.3 | – | – | – | 6.3 | 6.6 | 6.9 |
Std Deviation (1e-4) | 1.0 | 0.7 | 1.1 | 1.6 | 0.9 | 0.7 | 0.6 | 1.1 | 0.6 | 0.9 | 0.9 | 1.4 | 1.0 | 1.0 | 0.9 | – | – | – | 1.0 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.41 | -0.24 | -0.58 | -0.21 | -0.42 | -0.6 | -0.41 | -0.63 | 0.47 | 0.45 | 0.24 | – | – | – | 0.47 | 0.54 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | -0.9 | -4.1 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.0 | -5.6 | -5.2 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.1 | -4.8 | -5.0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | -5.5 | -5.5 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 4.1 | 4.1 | – | – | – |
Cytoplasm | 0 | 0.8 | 0.4 | 3.3 | -0.4 | -0.7 | 5.9 | -2.5 | 4.3 | 3.3 | 8.5 | 11.6 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.9 | -4.4 | -4.2 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | -3.5 | -4.1 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.6 | -7.1 | -7.9 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1579 | 2.7634 | 2.2494 | 1.7451 | 4.2768 | 2.8792 | 2.8365 | 4.2304 | 3.4688 | 3.7531 | 3.3916 | 3.8019 | 5.2839 | 5.7789 | 5.6211 | 5.3265 | 5.2351 | 5.5461 |
Actin | 0.0644 | 0.0193 | 0.0866 | 0.0013 | 0.0088 | 0.0503 | 0.0264 | 0.0161 | 0.042 | 0.0276 | 0.0473 | 0.0231 | 0.1095 | 0.0353 | 0.0707 | 0.0828 | 0.0629 | 0.0618 |
Bud | 0.0033 | 0.0021 | 0.0799 | 0.0046 | 0.0005 | 0.0009 | 0.0101 | 0.0218 | 0.0774 | 0.0421 | 0.0174 | 0.002 | 0.0093 | 0.0243 | 0.0836 | 0.0161 | 0.0417 | 0.005 |
Bud Neck | 0.0438 | 0.0098 | 0.001 | 0.0013 | 0.0009 | 0.4449 | 0.0535 | 0.0224 | 0.0061 | 0.0033 | 0.1495 | 0.5539 | 0.0504 | 0.014 | 0.0048 | 0.0045 | 0.0831 | 0.4509 |
Bud Periphery | 0.0132 | 0.0021 | 0.1483 | 0.0021 | 0.0004 | 0.0021 | 0.0177 | 0.0154 | 0.1543 | 0.0865 | 0.0668 | 0.0054 | 0.0178 | 0.0447 | 0.169 | 0.0419 | 0.1331 | 0.0113 |
Bud Site | 0.0454 | 0.3974 | 0.0135 | 0.0003 | 0.0014 | 0.005 | 0.1892 | 0.3283 | 0.0841 | 0.0317 | 0.0335 | 0.0058 | 0.2156 | 0.3192 | 0.0904 | 0.0186 | 0.0429 | 0.0127 |
Cell Periphery | 0.0061 | 0.005 | 0.0053 | 0.0004 | 0.0003 | 0.0014 | 0.0066 | 0.004 | 0.0045 | 0.003 | 0.0087 | 0.0024 | 0.0117 | 0.0068 | 0.0051 | 0.0056 | 0.0052 | 0.0044 |
Cytoplasm | 0.198 | 0.4217 | 0.6084 | 0.8161 | 0.4025 | 0.2421 | 0.2451 | 0.4121 | 0.4097 | 0.5474 | 0.3592 | 0.2341 | 0.4458 | 0.4308 | 0.4741 | 0.7147 | 0.5088 | 0.3343 |
Cytoplasmic Foci | 0.0552 | 0.0209 | 0.0106 | 0.0028 | 0.0781 | 0.0159 | 0.1153 | 0.0358 | 0.0218 | 0.0473 | 0.0578 | 0.0562 | 0.0435 | 0.041 | 0.0209 | 0.0177 | 0.0234 | 0.0413 |
Eisosomes | 0.0014 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0018 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0017 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0006 |
Endoplasmic Reticulum | 0.0216 | 0.0019 | 0.0043 | 0.0083 | 0.004 | 0.0585 | 0.0034 | 0.0029 | 0.0046 | 0.0058 | 0.0067 | 0.0053 | 0.0072 | 0.0037 | 0.0042 | 0.0038 | 0.0056 | 0.0018 |
Endosome | 0.0531 | 0.0146 | 0.0068 | 0.0029 | 0.1121 | 0.0327 | 0.0258 | 0.0023 | 0.0107 | 0.0243 | 0.0588 | 0.0042 | 0.0124 | 0.0159 | 0.0127 | 0.0058 | 0.0207 | 0.0089 |
Golgi | 0.0422 | 0.0238 | 0.0035 | 0.0004 | 0.2395 | 0.0438 | 0.01 | 0.0013 | 0.0059 | 0.0082 | 0.0181 | 0.0043 | 0.0112 | 0.0034 | 0.0062 | 0.0043 | 0.0098 | 0.006 |
Lipid Particles | 0.1302 | 0.0066 | 0.0004 | 0.0001 | 0.0128 | 0.0183 | 0.013 | 0.0007 | 0.0061 | 0.0036 | 0.012 | 0.002 | 0.0043 | 0.0007 | 0.0004 | 0.0005 | 0.0072 | 0.0007 |
Mitochondria | 0.0841 | 0.0022 | 0.0015 | 0.0005 | 0.0295 | 0.041 | 0.0125 | 0.0003 | 0.006 | 0.0033 | 0.0187 | 0.0016 | 0.0144 | 0.0011 | 0.0031 | 0.0015 | 0.0089 | 0.0012 |
None | 0.1133 | 0.0536 | 0.0223 | 0.1532 | 0.0915 | 0.0239 | 0.2049 | 0.1267 | 0.1492 | 0.1447 | 0.1147 | 0.0903 | 0.0304 | 0.0504 | 0.044 | 0.0696 | 0.0163 | 0.0536 |
Nuclear Periphery | 0.012 | 0.0008 | 0.0008 | 0.001 | 0.0003 | 0.0024 | 0.0014 | 0.0004 | 0.0011 | 0.0014 | 0.0017 | 0.0004 | 0.0008 | 0.0006 | 0.0007 | 0.0007 | 0.0016 | 0.0002 |
Nucleolus | 0.0081 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0039 | 0.0003 | 0.0006 | 0.0007 | 0.0015 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0002 |
Nucleus | 0.0217 | 0.0027 | 0.0011 | 0.0017 | 0.0002 | 0.0014 | 0.0029 | 0.0012 | 0.002 | 0.0019 | 0.0024 | 0.0007 | 0.0012 | 0.0012 | 0.0011 | 0.0018 | 0.0016 | 0.0006 |
Peroxisomes | 0.0207 | 0.0039 | 0.0014 | 0 | 0.0142 | 0.0075 | 0.0395 | 0.0027 | 0.0042 | 0.0036 | 0.005 | 0.0053 | 0.0069 | 0.0017 | 0.0031 | 0.0012 | 0.0124 | 0.0024 |
Punctate Nuclear | 0.0562 | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0028 | 0.0115 | 0.0016 | 0.0012 | 0.0046 | 0.013 | 0.0016 | 0.0023 | 0.0014 | 0.0007 | 0.004 | 0.0065 | 0.0007 |
Vacuole | 0.0038 | 0.0073 | 0.0034 | 0.0022 | 0.0017 | 0.0017 | 0.005 | 0.003 | 0.0072 | 0.0074 | 0.0049 | 0.001 | 0.0027 | 0.0028 | 0.0041 | 0.0038 | 0.0062 | 0.0013 |
Vacuole Periphery | 0.002 | 0.0004 | 0.0004 | 0.0006 | 0.0008 | 0.0014 | 0.0008 | 0.0001 | 0.0009 | 0.0011 | 0.0022 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0016 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.7639 | 31.9706 | 29.9666 | 34.3374 | 32.9465 | 30.2883 | 36.9616 | 35.884 | 36.3534 | 40.4993 |
Translational Efficiency | 0.6092 | 0.4509 | 0.4385 | 0.4066 | 0.3751 | 0.4925 | 0.4211 | 0.3709 | 0.364 | 0.3688 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
165 | 880 | 149 | 1119 | 1783 | 1411 | 1380 | 1399 | 1948 | 2291 | 1529 | 2518 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 630.64 | 851.71 | 1234.46 | 953.46 | 797.21 | 766.85 | 947.31 | 732.34 | 783.10 | 799.45 | 975.29 | 830.61 |
Standard Deviation | 70.72 | 105.80 | 125.05 | 180.23 | 86.50 | 106.10 | 114.47 | 157.45 | 97.07 | 113.74 | 143.53 | 200.70 |
Intensity Change Log 2 | — | 0.433546 | 0.968992 | 0.596356 | — | -0.056015 | 0.248877 | -0.122446 | — | 0.180866 | 0.611655 | 0.239589 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006817 | 0.006743 | 0.087108 | 0.022951 | 0.010280 | 0.004911 | 0.014467 | 0.005862 | 0.009987 | 0.005615 | 0.021546 | 0.013456 |
Bud Neck | 0.108411 | 0.093241 | 0.037338 | 0.044205 | 0.163650 | 0.090335 | 0.081479 | 0.023716 | 0.158971 | 0.091451 | 0.077178 | 0.032822 |
Bud Site | 0.089421 | 0.298060* | 0.281170* | 0.411555* | 0.271318* | 0.265088* | 0.430943* | 0.188502* | 0.255911* | 0.277753* | 0.416348* | 0.287627* |
Cell Periphery | 0.005493 | 0.001008 | 0.003895 | 0.000681 | 0.002035 | 0.000465 | 0.000713 | 0.003750 | 0.002328 | 0.000674 | 0.001024 | 0.002386 |
Cytoplasm | 0.347508* | 0.329751* | 0.142692* | 0.337644* | 0.351534* | 0.381364* | 0.314902* | 0.425777* | 0.351193* | 0.361539* | 0.298120* | 0.386611* |
Cytoplasmic Foci | 0.219710* | 0.153230 | 0.089886 | 0.036408 | 0.103559 | 0.132358 | 0.032813 | 0.036373 | 0.113397 | 0.140375 | 0.038375 | 0.036388 |
Eisosomes | 0.002719 | 0.000660 | 0.001695 | 0.000280 | 0.002692 | 0.000385 | 0.000224 | 0.000275 | 0.002695 | 0.000491 | 0.000368 | 0.000277 |
Endoplasmic Reticulum | 0.005950 | 0.001488 | 0.005948 | 0.000718 | 0.001906 | 0.000952 | 0.000845 | 0.001291 | 0.002248 | 0.001158 | 0.001342 | 0.001036 |
Endosome | 0.020343 | 0.025962 | 0.015249 | 0.006651 | 0.012516 | 0.021456 | 0.004718 | 0.010029 | 0.013179 | 0.023187 | 0.005744 | 0.008528 |
Golgi | 0.012477 | 0.015175 | 0.001503 | 0.003277 | 0.011325 | 0.013193 | 0.000886 | 0.007942 | 0.011423 | 0.013954 | 0.000946 | 0.005869 |
Lipid Particles | 0.018740 | 0.004264 | 0.006288 | 0.000687 | 0.003059 | 0.004526 | 0.000641 | 0.001592 | 0.004388 | 0.004425 | 0.001191 | 0.001190 |
Mitochondria | 0.022116 | 0.004927 | 0.000034 | 0.002020 | 0.007009 | 0.006932 | 0.000507 | 0.003758 | 0.008288 | 0.006162 | 0.000461 | 0.002986 |
Mitotic Spindle | 0.000600 | 0.002664 | 0.000580 | 0.007710 | 0.001922 | 0.003602 | 0.003885 | 0.009348 | 0.001810 | 0.003241 | 0.003562 | 0.008620 |
None | 0.001238 | 0.000307 | 0.000033 | 0.001613 | 0.000316 | 0.000667 | 0.000413 | 0.024614 | 0.000394 | 0.000529 | 0.000376 | 0.014393 |
Nuclear Periphery | 0.000396 | 0.000037 | 0.000148 | 0.000070 | 0.000025 | 0.000070 | 0.000060 | 0.000670 | 0.000056 | 0.000057 | 0.000068 | 0.000404 |
Nuclear Periphery Foci | 0.000643 | 0.000355 | 0.008043 | 0.000467 | 0.000141 | 0.000217 | 0.001022 | 0.001329 | 0.000183 | 0.000270 | 0.001706 | 0.000946 |
Nucleolus | 0.003383 | 0.000396 | 0.000054 | 0.000111 | 0.000134 | 0.000278 | 0.000079 | 0.000876 | 0.000409 | 0.000324 | 0.000077 | 0.000536 |
Nucleus | 0.061892 | 0.006048 | 0.013375 | 0.009031 | 0.013689 | 0.008284 | 0.011998 | 0.156363* | 0.017771 | 0.007425 | 0.012133 | 0.090888 |
Peroxisomes | 0.042659 | 0.013085 | 0.011256 | 0.011600 | 0.009078 | 0.014274 | 0.006340 | 0.003838 | 0.011923 | 0.013817 | 0.006819 | 0.007287 |
Vacuole | 0.026245 | 0.039811 | 0.293509* | 0.101283 | 0.032418 | 0.048330 | 0.092765 | 0.091091 | 0.031895 | 0.045058 | 0.112327 | 0.095621 |
Vacuole Periphery | 0.003238 | 0.002788 | 0.000194 | 0.001037 | 0.001396 | 0.002312 | 0.000299 | 0.003004 | 0.001552 | 0.002495 | 0.000289 | 0.002130 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.02 | -4.95 | -4.52 | -5.21 | 3.83 | 4.84 | -1.58 | 5.32 | 1.08 | 5.54 | 4.19 | -4.17 | -0.89 | -4.39 | 3.32 |
Bud Neck | 0.87 | 4.47 | 4.59 | 9.05 | -0.66 | 9.72 | 12.70 | 23.51 | 15.43 | 12.09 | 10.03 | 13.45 | 22.74 | 17.21 | 10.09 |
Bud Site | -12.92 | -5.66 | -15.01 | -2.40 | -4.28 | 0.68 | -9.36 | 15.86* | 13.90 | 22.54* | -2.37 | -10.05 | 4.56 | 7.02 | 13.92 |
Cell Periphery | 1.45 | 0.71 | 1.59 | 3.03 | 3.42 | 11.82 | 10.32 | -0.50 | -3.05 | -2.75 | 5.71 | 4.80 | 1.69 | -2.58 | -1.89 |
Cytoplasm | 0.82 | 8.30 | -0.51 | -2.11 | -12.27 | -2.90 | 1.62 | -2.91 | 0.06 | -4.17 | -1.21 | 3.43 | -2.25 | -1.05 | -5.55 |
Cytoplasmic Foci | 4.07 | 8.02 | 11.78 | 16.36 | 5.62 | -4.93 | 16.25 | 18.52 | 20.60 | 2.52 | -5.17 | 17.52 | 20.39 | 26.02 | 2.89 |
Eisosomes | 4.21 | 2.03 | 5.15 | 3.49 | 2.56 | 7.69 | 8.36 | 8.41 | 3.30 | 0.49 | 7.91 | 8.52 | 8.99 | 4.71 | 2.01 |
Endoplasmic Reticulum | 2.09 | 0.09 | 2.47 | 1.95 | 7.65 | 3.44 | 3.87 | -0.44 | -5.63 | -7.54 | 3.36 | 2.80 | 2.78 | -1.56 | -0.28 |
Endosome | -1.39 | 1.53 | 3.91 | 9.62 | 4.53 | -5.44 | 7.67 | 4.69 | 8.90 | -2.51 | -7.09 | 7.59 | 6.59 | 13.05 | -1.05 |
Golgi | -1.05 | 4.78 | 4.07 | 9.25 | -1.68 | -1.54 | 13.93 | 4.46 | 5.31 | -3.28 | -2.47 | 14.71 | 7.35 | 9.57 | -3.86 |
Lipid Particles | 4.63 | 4.27 | 6.14 | 3.04 | 7.52 | -1.88 | 10.57 | 5.79 | 3.69 | -5.91 | -0.09 | 9.11 | 9.24 | 4.86 | -0.14 |
Mitochondria | 2.64 | 3.43 | 3.18 | 3.11 | -2.80 | 0.05 | 7.49 | 4.02 | 3.83 | -3.17 | 1.81 | 8.10 | 5.79 | 5.02 | -3.75 |
Mitotic Spindle | -2.61 | -0.29 | -4.79 | -3.04 | -4.67 | -1.60 | -1.88 | -4.01 | -3.00 | -2.72 | -1.72 | -1.86 | -5.20 | -4.12 | -3.41 |
None | 3.51 | 4.65 | -0.07 | -1.47 | -1.89 | -2.77 | -0.89 | -6.34 | -6.19 | -6.30 | -1.57 | 0.12 | -6.42 | -6.32 | -6.42 |
Nuclear Periphery | 3.17 | 1.40 | 2.60 | -3.40 | 3.19 | -2.14 | -9.61 | -20.05 | -18.39 | -18.87 | -0.07 | -4.12 | -18.13 | -17.65 | -17.11 |
Nuclear Periphery Foci | 1.06 | -7.71 | -0.00 | -2.66 | 7.93 | -1.71 | -6.98 | -5.84 | -5.37 | -0.43 | -2.09 | -9.72 | -6.88 | -6.03 | 4.17 |
Nucleolus | 1.31 | 1.48 | 1.46 | 1.16 | -1.08 | -1.68 | 1.83 | -3.16 | -2.23 | -3.41 | 0.31 | 1.69 | -0.29 | -0.84 | -3.45 |
Nucleus | 7.03 | 5.14 | 6.11 | -5.29 | 0.45 | 4.96 | -2.08 | -29.88 | -30.73 | -29.50 | 8.50 | 1.07 | -24.77 | -27.54 | -25.29 |
Peroxisomes | 3.38 | 3.78 | 3.75 | 1.79 | -0.28 | -3.44 | 3.48 | 6.93 | 7.92 | 3.94 | -1.38 | 4.99 | 5.06 | 6.81 | 0.49 |
Vacuole | -3.11 | -14.13 | -17.84 | -16.78 | 7.89 | -5.85 | -17.85 | -21.19 | -17.60 | -3.53 | -5.97 | -21.07 | -27.70 | -24.18 | -1.39 |
Vacuole Periphery | 0.39 | 2.91 | 2.22 | 3.67 | -1.99 | -2.12 | 6.27 | -0.69 | 0.51 | -2.28 | -2.73 | 6.91 | 0.30 | 2.10 | -2.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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