Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0.08 0.06 0.07 0.08 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.95 0.87 0.8 0.73 0.68 0.68 0.69 0.6 0.99 0.98 0.98 0.98 0.98 0.98 0.97 0.94 0.95 0.94 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0.11 0.15 0.23 0.24 0.2 0.17 0.27 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 13 11 21 23 18 25 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 2 1 0 0 1 2 1 0 3 4 1 0 0 1 0 0 0 1 0 2 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 3 6 11 9 25 26 32 27 24 34 2 1 4 8 8 12 0 0 0 0 0 0
Cytoplasm 339 99 150 277 376 288 387 359 271 312 249 265 164 346 377 267 211 184 326 93 140 143 305 306
Endoplasmic Reticulum 2 1 1 4 17 35 71 114 95 93 63 118 1 10 7 12 10 6 4 0 2 2 7 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 5 5 5 5 7 14 11 12 0 0 1 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3 2 14 12 17 15 0 0 0 0 0 0 0 0 0 0 1 0
Nucleus 0 0 0 1 0 1 1 1 1 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 2 1 2 4 3 2 19 6 21 0 0 3 2 1 1 0 0 0 1 1 2
Unique Cell Count 341 101 154 283 396 332 481 495 400 457 360 440 165 352 384 272 215 188 335 99 147 153 323 332
Labelled Cell Count 343 102 157 290 410 346 511 522 446 504 393 494 167 357 393 290 231 203 335 99 147 153 323 332


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 70.5 91.1 68.1 67.5 53.3 39.7 34.5 34.0 28.8 27.7 25.1 24.6 82.5 79.0 76.3 109.4 130.7 132.7 76.2 81.2 86.9
Std Deviation (1e-4) 13.8 22.2 15.5 15.0 9.9 8.8 8.0 7.0 6.4 6.2 6.1 5.2 19.4 18.5 18.3 27.9 31.8 30.6 21.6 22.7 24.5
Intensity Change (Log2) – – – -0.01 -0.36 -0.78 -0.98 -1.0 -1.24 -1.3 -1.44 -1.47 0.28 0.21 0.16 0.68 0.94 0.96 0.16 0.25 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 2.9 2.8 2.8 3.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.7 1.7 2.6 2.0 2.2 2.6 0 0 0 0 0 2.0
Cytoplasm 0.3 -1.3 -3.7 -5.1 -6.6 -7.3 -7.3 -7.0 -8.7 1.4 0.7 0.6 0.5 0.5 0.3
Endoplasmic Reticulum 0 2.2 3.9 4.8 6.4 6.4 5.9 5.3 7.0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.1 0 2.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 57.7442 75.8009 69.3443 58.8915 63.5618 66.8167 55.5187 84.0174 72.3325 65.1116 60.4982 64.0273 62.1331 79.0967 75.8978 69.1106 63.252 66.8636
Actin 0.0007 0.0001 0.0001 0.0136 0.0001 0.0002 0.0118 0.0001 0.01 0.0111 0.0436 0.0164 0.0013 0.0011 0.0049 0.0003 0.0044 0.0305
Bud 0.0004 0.0001 0.0001 0.0008 0.0002 0.0001 0.0004 0.0001 0.0005 0.0013 0.0005 0.0004 0.0005 0.0001 0.0002 0.0006 0.0002 0.0003
Bud Neck 0.0004 0.0002 0.0003 0.0006 0.0009 0.0007 0.0011 0.0001 0.001 0.0013 0.0014 0.001 0.0002 0.0002 0.0002 0.0003 0.0009 0.0004
Bud Periphery 0.0006 0.0001 0.0001 0.001 0.0002 0.0002 0.0005 0 0.0003 0.0016 0.0007 0.0002 0.0006 0 0.0002 0.0005 0.0003 0.0002
Bud Site 0.0002 0.0001 0 0.0007 0.0001 0 0.0024 0.0001 0.0006 0.006 0.0012 0.0004 0.0002 0.0001 0.0003 0.0009 0.0021 0.0003
Cell Periphery 0.0042 0.0031 0.0014 0.0027 0.0034 0.002 0.0013 0.0002 0.0003 0.0002 0.0001 0.0001 0.0013 0.0013 0.0015 0.003 0.0002 0
Cytoplasm 0.8802 0.9632 0.9388 0.8915 0.9311 0.9248 0.8257 0.9902 0.8893 0.9022 0.7253 0.9233 0.8862 0.9732 0.9482 0.893 0.8589 0.9283
Cytoplasmic Foci 0.005 0.0006 0.0011 0.0016 0.0011 0.0042 0.0152 0.0006 0.0123 0.0076 0.0085 0.0067 0.0027 0.0006 0.0054 0.0033 0.016 0.0027
Eisosomes 0.0001 0 0 0.0003 0 0 0.0001 0 0.0002 0.0001 0.0004 0.0001 0 0 0.0001 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0456 0.0185 0.0246 0.012 0.0069 0.0381 0.0195 0.0036 0.014 0.0094 0.0327 0.0083 0.0303 0.0151 0.0067 0.017 0.0066 0.0086
Endosome 0.0047 0.0009 0.0021 0.0066 0.0055 0.0033 0.0172 0.0008 0.005 0.0079 0.0115 0.0068 0.0058 0.0006 0.0037 0.0055 0.0176 0.0066
Golgi 0.0006 0.0001 0.0001 0.0014 0.0001 0.0006 0.0043 0.0001 0.0024 0.0036 0.0085 0.0019 0.0005 0.0002 0.003 0.0003 0.005 0.0017
Lipid Particles 0.0015 0.0013 0.0008 0.0008 0.0008 0.0003 0.0142 0.0001 0.005 0.0037 0.014 0.003 0.0009 0.0005 0.0021 0.0024 0.0249 0.0018
Mitochondria 0.0028 0.0004 0.0002 0.0046 0.0004 0.0018 0.0036 0 0.0079 0.0039 0.0674 0.0007 0.0024 0.0001 0.0024 0.0049 0.0057 0.0016
None 0.003 0.0001 0.0003 0.0015 0.0003 0.0012 0.0044 0.0001 0.0019 0.0011 0.0032 0.0015 0.0019 0.0001 0.0013 0.0016 0.0029 0.0003
Nuclear Periphery 0.0078 0.0016 0.004 0.0077 0.0058 0.0054 0.0187 0.001 0.0074 0.0033 0.0492 0.0075 0.0204 0.0021 0.0039 0.0065 0.0221 0.0065
Nucleolus 0.0019 0 0.0027 0.0013 0.0002 0.0021 0.0041 0 0.0012 0.0004 0.0005 0.0003 0.0008 0.0001 0.0031 0.0015 0.0014 0
Nucleus 0.005 0.001 0.0032 0.0062 0.0062 0.0021 0.0091 0.0007 0.0032 0.0021 0.0015 0.0029 0.0057 0.0007 0.0023 0.0033 0.0023 0.0021
Peroxisomes 0.0005 0 0 0.0002 0 0.0001 0.007 0 0.0131 0.0166 0.0131 0.0016 0.0004 0 0.0008 0.0002 0.009 0.0023
Punctate Nuclear 0.0033 0 0.0001 0.0004 0 0.0002 0.0155 0 0.0014 0.0022 0.0061 0.0118 0.0007 0 0.0003 0.0001 0.0051 0.0001
Vacuole 0.0267 0.008 0.0175 0.0396 0.0294 0.0113 0.0191 0.002 0.016 0.012 0.0074 0.0042 0.0274 0.0037 0.0075 0.043 0.01 0.0035
Vacuole Periphery 0.0048 0.0004 0.0025 0.0051 0.0072 0.0013 0.0047 0.0002 0.0069 0.0026 0.0034 0.0011 0.0096 0.0003 0.0015 0.0116 0.0041 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 941.1391 1114.7282 1458.6486 1000.9419 1290.356 973.0532 944.88 866.9053 1108.5895 813.2231
Translational Efficiency 1.0269 1.0542 0.8869 1.3469 0.9628 0.9178 1.1151 1.2188 0.9852 1.2643

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl18b

Rpl18b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl18b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available