Standard name | |
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Human Ortholog | |
Description | Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 5 | 1 | 0 | 1 | – | 0 | 6 | 0 | 3 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | – | 0 | 1 | 2 | 4 | – | 0 | 11 | 2 | 5 | – | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | – | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | – | 11 | 38 | 23 | 74 | – | 42 | 64 | 30 | 28 | – | 6 | 24 | 28 | 27 | 43 | 0 | 0 | 0 | 0 | 4 | 2 |
Endoplasmic Reticulum | 1 | 0 | – | 1 | 0 | 1 | 0 | – | 0 | 3 | 3 | 4 | – | 2 | 2 | 3 | 6 | 9 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 16 | 34 | – | 22 | 55 | 32 | 70 | – | 32 | 74 | 11 | 17 | – | 59 | 117 | 60 | 61 | 75 | 69 | 56 | 12 | 12 | 93 | 141 |
Golgi | 0 | 1 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 6 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 11 | 7 | – | 3 | 0 | 7 | 4 | – | 13 | 24 | 40 | 23 | – | 9 | 2 | 5 | 14 | 5 | 0 | 0 | 0 | 0 | 3 | 2 |
Nucleus | 1 | 0 | – | 4 | 4 | 2 | 4 | – | 5 | 2 | 1 | 1 | – | 2 | 3 | 0 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 3 |
Nuclear Periphery | 2 | 0 | – | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 23 | 28 | – | 20 | 17 | 10 | 13 | – | 3 | 2 | 12 | 4 | – | 19 | 21 | 17 | 16 | 20 | 1 | 1 | 0 | 0 | 1 | 3 |
Peroxisomes | 0 | 0 | – | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 15 | – | 12 | 25 | 3 | 27 | – | 9 | 24 | 9 | 7 | – | 24 | 46 | 19 | 21 | 17 | 0 | 6 | 1 | 1 | 12 | 3 |
Vac/Vac Membrane | 45 | 60 | – | 27 | 66 | 46 | 64 | – | 25 | 66 | 19 | 34 | – | 27 | 41 | 61 | 75 | 101 | 15 | 33 | 7 | 3 | 34 | 41 |
Unique Cell Count | 74 | 93 | 79 | 152 | 105 | 205 | 110 | 226 | 103 | 106 | 108 | 181 | 120 | 148 | 185 | 91 | 104 | 26 | 21 | 160 | 208 | |||
Labelled Cell Count | 109 | 153 | 106 | 209 | 128 | 263 | 133 | 278 | 127 | 129 | 151 | 261 | 199 | 233 | 276 | 91 | 104 | 26 | 21 | 160 | 208 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 7.6 | – | 7.2 | 8.3 | 6.5 | 7.1 | – | 6.4 | 6.3 | 5.8 | 5.5 | – | 7.1 | 7.4 | 9.2 | 9.9 | 9.4 | – | 6.5 | 7.7 |
Std Deviation (1e-4) | 0.8 | 1.6 | – | 1.6 | 1.8 | 1.4 | 4.1 | – | 1.6 | 1.3 | 1.2 | 0.8 | – | 1.3 | 1.1 | 2.4 | 2.6 | 2.5 | – | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3302 | 2.1463 | 1.5653 | 1.1821 | 0.3184 | 1.7176 | 0.9031 | 2.8862 | 2.3291 | 1.6206 | 1.1834 | 2.3183 | 0.4391 | 1.3921 | 1.0147 | 1.089 | 1.6258 | 1.1527 |
Actin | 0.0524 | 0 | 0.0042 | 0 | 0.0001 | 0.0003 | 0.0636 | 0.0001 | 0.0022 | 0 | 0.0001 | 0 | 0.073 | 0 | 0.0168 | 0.0002 | 0.0008 | 0.0004 |
Bud | 0.0009 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0006 | 0.0002 | 0.0012 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0002 |
Bud Neck | 0.0027 | 0.0008 | 0.0008 | 0.0002 | 0.0003 | 0.0007 | 0.0012 | 0.0125 | 0.0009 | 0.0004 | 0.0019 | 0.0009 | 0.0026 | 0.0011 | 0.0007 | 0.0009 | 0.0004 | 0.0012 |
Bud Periphery | 0.001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0008 | 0.0002 | 0.0013 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0003 |
Bud Site | 0.0061 | 0.0006 | 0.0024 | 0.0001 | 0.0006 | 0.0003 | 0.0044 | 0.0148 | 0.001 | 0.0002 | 0.0027 | 0.0008 | 0.0152 | 0.0007 | 0.0034 | 0.0004 | 0.0003 | 0.0017 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
Cytoplasm | 0.014 | 0.0199 | 0.0046 | 0.0008 | 0.0143 | 0.0038 | 0.0063 | 0.0199 | 0.0501 | 0.0024 | 0.0129 | 0.0108 | 0.0093 | 0.0134 | 0.0384 | 0.0064 | 0.0081 | 0.008 |
Cytoplasmic Foci | 0.133 | 0.0962 | 0.0852 | 0.0413 | 0.0443 | 0.0716 | 0.1013 | 0.1005 | 0.0587 | 0.0536 | 0.0484 | 0.0781 | 0.1553 | 0.097 | 0.0743 | 0.0272 | 0.108 | 0.0743 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0013 | 0.0008 | 0.0003 | 0.002 | 0.0008 | 0.0022 | 0.002 | 0.0015 | 0.0002 | 0.0015 | 0.0013 | 0.0032 | 0.0012 | 0.001 | 0.0006 | 0.0005 | 0.0009 |
Endosome | 0.4276 | 0.421 | 0.4704 | 0.4338 | 0.5442 | 0.4941 | 0.5328 | 0.337 | 0.433 | 0.5061 | 0.5629 | 0.5564 | 0.3596 | 0.3942 | 0.3908 | 0.4373 | 0.6261 | 0.4021 |
Golgi | 0.055 | 0.0096 | 0.0304 | 0.0066 | 0.008 | 0.0167 | 0.0246 | 0.011 | 0.0307 | 0.0051 | 0.0419 | 0.0156 | 0.0325 | 0.0121 | 0.0246 | 0.027 | 0.0601 | 0.0176 |
Lipid Particles | 0.1074 | 0.0527 | 0.0686 | 0.0163 | 0.0055 | 0.0964 | 0.1083 | 0.0391 | 0.0145 | 0.0086 | 0.1148 | 0.0117 | 0.0581 | 0.0461 | 0.062 | 0.006 | 0.0343 | 0.0692 |
Mitochondria | 0.0256 | 0.0011 | 0.0308 | 0.0002 | 0.0019 | 0.0058 | 0.0079 | 0.0006 | 0.003 | 0.0002 | 0.0076 | 0.004 | 0.0421 | 0.0009 | 0.0239 | 0.0798 | 0.0276 | 0.0299 |
None | 0.0043 | 0.0005 | 0.0011 | 0 | 0.0046 | 0.0007 | 0.0022 | 0.0008 | 0.0035 | 0.0001 | 0.0008 | 0.0253 | 0.0833 | 0.0026 | 0.0215 | 0.0004 | 0.0002 | 0.0002 |
Nuclear Periphery | 0.0049 | 0.0033 | 0.0018 | 0.001 | 0.0196 | 0.0113 | 0.0023 | 0.0061 | 0.0029 | 0.0005 | 0.0025 | 0.0032 | 0.0047 | 0.0044 | 0.0022 | 0.0015 | 0.0009 | 0.0024 |
Nucleolus | 0.0019 | 0.0024 | 0.0018 | 0.0003 | 0.0048 | 0.0089 | 0.0016 | 0.0117 | 0.0021 | 0.0014 | 0.0048 | 0.0011 | 0.0027 | 0.0042 | 0.0034 | 0.0007 | 0.0003 | 0.0024 |
Nucleus | 0.0013 | 0.002 | 0.0009 | 0.0004 | 0.0873 | 0.0028 | 0.0009 | 0.0266 | 0.0021 | 0.0006 | 0.0065 | 0.0013 | 0.0032 | 0.0027 | 0.0131 | 0.0009 | 0.0008 | 0.0022 |
Peroxisomes | 0.0432 | 0.0073 | 0.012 | 0.0008 | 0.001 | 0.0025 | 0.0173 | 0.0133 | 0.0167 | 0.0018 | 0.0041 | 0.002 | 0.008 | 0.0067 | 0.0131 | 0.0011 | 0.0053 | 0.0037 |
Punctate Nuclear | 0.0039 | 0.0005 | 0.0008 | 0.0001 | 0.0032 | 0.0037 | 0.0023 | 0.019 | 0.0003 | 0.0002 | 0.0007 | 0.0004 | 0.0483 | 0.0007 | 0.0011 | 0.0002 | 0.0004 | 0.0011 |
Vacuole | 0.0897 | 0.2951 | 0.2233 | 0.3459 | 0.1949 | 0.1923 | 0.104 | 0.3275 | 0.3158 | 0.3716 | 0.1635 | 0.237 | 0.0728 | 0.3218 | 0.2451 | 0.3363 | 0.1118 | 0.2901 |
Vacuole Periphery | 0.0177 | 0.0852 | 0.0591 | 0.1517 | 0.0633 | 0.0866 | 0.015 | 0.0562 | 0.0601 | 0.0468 | 0.0207 | 0.0498 | 0.023 | 0.0892 | 0.0634 | 0.0723 | 0.0136 | 0.0917 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.1208 | 20.5825 | 13.315 | 20.0755 | 15.6916 | 20.0615 | 24.0126 | 24.8506 | 23.8756 | 19.868 |
Translational Efficiency | 1.6306 | 1.5588 | 1.8378 | 1.3974 | 1.5427 | 1.7642 | 1.1911 | 1.4193 | 1.3103 | 1.48 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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