Standard name | |
---|---|
Human Ortholog | |
Description | Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 8 | 10 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 2 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | 8 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 191 | 204 | 140 | 167 | 312 | 221 | 231 | 282 | 185 | 201 | 183 | 173 | 382 | 576 | 544 | 136 | 144 | 128 | 179 | 191 | 143 | 175 | 382 | 459 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 1 | 3 | 3 | 0 | 4 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 2 | 3 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 2 | 0 | 2 | 3 | 28 | 68 | 57 | 56 | 59 | 121 | 78 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 3 | 2 | 0 | 1 | 0 |
Nucleus | 0 | 1 | 1 | 5 | 2 | 4 | 12 | 10 | 13 | 9 | 17 | 16 | 3 | 5 | 2 | 1 | 0 | 3 | 0 | 5 | 2 | 1 | 0 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 7 | 8 | 22 | 24 | 22 | 33 | 12 | 37 | 31 | 51 | 10 | 12 | 18 | 13 | 21 | 10 | 0 | 1 | 2 | 1 | 1 | 3 |
Unique Cell Count | 193 | 206 | 144 | 172 | 327 | 259 | 299 | 359 | 244 | 277 | 296 | 269 | 389 | 582 | 555 | 146 | 160 | 145 | 189 | 206 | 160 | 185 | 395 | 477 |
Labelled Cell Count | 195 | 209 | 148 | 183 | 342 | 282 | 342 | 387 | 275 | 316 | 364 | 319 | 398 | 595 | 569 | 162 | 178 | 158 | 189 | 206 | 160 | 185 | 395 | 477 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 8.7 | 7.6 | 7.3 | 7.3 | 6.1 | 5.6 | 6.1 | 5.3 | 5.8 | 5.0 | 5.4 | 7.6 | 8.0 | 8.0 | 12.1 | 11.9 | 11.9 | 7.8 | 8.5 | 8.9 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.0 | 0.8 | 1.7 | 1.3 | 1.2 | 1.2 | 1.4 | 1.5 | 1.1 | 0.8 | 0.9 | 0.9 | 0.9 | 2.3 | 1.9 | 2.2 | 1.2 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.06 | -0.31 | -0.44 | -0.33 | -0.52 | -0.39 | -0.6 | -0.49 | 0.0 | 0.08 | 0.08 | 0.67 | 0.64 | 0.64 | 0.03 | 0.16 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.1 | -0.9 | -3.7 | -5.3 | -5.1 | -5.5 | -6.1 | -7.9 | -7.4 | 0.7 | 1.6 | 0.6 | -1.6 | -2.5 | -2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.1 | 6.2 | 5.1 | 6.2 | 6.0 | 9.0 | 7.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 2.5 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.1 | 0.8 | 1.6 | 1.0 | 1.6 | 0 | 2.7 | 2.0 | 3.9 | 0 | 0 | -0.9 | 1.4 | 2.5 | 0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2027 | 1.9543 | 1.7884 | 1.4478 | 1.1279 | 1.6921 | 2.2925 | 2.9235 | 2.5722 | 1.6498 | 2.7579 | 2.4716 | 2.1076 | 2.7938 | 2.4006 | 2.5615 | 2.4073 | 2.1161 |
Actin | 0.014 | 0.0011 | 0.0104 | 0.0138 | 0.0015 | 0.008 | 0.023 | 0.0012 | 0.0139 | 0.0012 | 0.0523 | 0.0084 | 0.0319 | 0.0009 | 0.0335 | 0.0364 | 0.0437 | 0.0044 |
Bud | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0026 | 0.0006 | 0.002 | 0.0037 | 0.0031 | 0.0001 | 0.0036 | 0.0006 | 0.0044 | 0.0005 | 0.0044 | 0.0063 | 0.0348 | 0.0054 |
Bud Neck | 0.002 | 0.0004 | 0.0009 | 0.0007 | 0.0007 | 0.0024 | 0.0097 | 0.0004 | 0.0012 | 0.0005 | 0.0017 | 0.0149 | 0.0017 | 0.0004 | 0.005 | 0.0014 | 0.0012 | 0.0013 |
Bud Periphery | 0.0006 | 0.0002 | 0.0011 | 0.0003 | 0.0012 | 0.0006 | 0.0019 | 0.0011 | 0.0022 | 0 | 0.0043 | 0.0004 | 0.005 | 0.0004 | 0.0015 | 0.0038 | 0.0114 | 0.0021 |
Bud Site | 0.0028 | 0.0017 | 0.0007 | 0.0008 | 0.0056 | 0.0006 | 0.0085 | 0.0057 | 0.0086 | 0.0003 | 0.0047 | 0.0003 | 0.0041 | 0.004 | 0.0083 | 0.0119 | 0.0041 | 0.0003 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0012 | 0.0002 | 0.0005 | 0.0005 | 0.0005 | 0.0002 |
Cytoplasm | 0.6262 | 0.7955 | 0.7519 | 0.7192 | 0.667 | 0.781 | 0.6033 | 0.8385 | 0.8172 | 0.8446 | 0.544 | 0.8122 | 0.7362 | 0.8117 | 0.7424 | 0.6733 | 0.6887 | 0.8193 |
Cytoplasmic Foci | 0.0292 | 0.0101 | 0.0122 | 0.0144 | 0.0122 | 0.0253 | 0.0546 | 0.005 | 0.0115 | 0.0064 | 0.0413 | 0.0167 | 0.0223 | 0.003 | 0.0151 | 0.0469 | 0.0142 | 0.0204 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0046 | 0.0027 | 0.0031 | 0.0028 | 0.0028 | 0.0038 | 0.0067 | 0.0024 | 0.0025 | 0.0047 | 0.0055 | 0.0034 | 0.0084 | 0.0048 | 0.0092 | 0.0071 | 0.0164 | 0.0036 |
Endosome | 0.0217 | 0.0058 | 0.0058 | 0.0052 | 0.005 | 0.0137 | 0.0381 | 0.0012 | 0.0024 | 0.0032 | 0.0354 | 0.0099 | 0.0134 | 0.0011 | 0.014 | 0.0192 | 0.0157 | 0.0113 |
Golgi | 0.0051 | 0.0003 | 0.0013 | 0.0012 | 0.0003 | 0.0014 | 0.009 | 0.0002 | 0.0007 | 0.0003 | 0.0188 | 0.0015 | 0.0015 | 0.0001 | 0.0057 | 0.0094 | 0.0068 | 0.0022 |
Lipid Particles | 0.0083 | 0.0001 | 0.001 | 0.0003 | 0.0002 | 0.001 | 0.0108 | 0.0001 | 0.0005 | 0.0003 | 0.0467 | 0.0011 | 0.0008 | 0.0001 | 0.0012 | 0.0055 | 0.0041 | 0.001 |
Mitochondria | 0.0079 | 0.0005 | 0.0028 | 0.0011 | 0.0005 | 0.0017 | 0.0092 | 0.0003 | 0.0008 | 0.0003 | 0.0053 | 0.0006 | 0.0021 | 0.0002 | 0.0037 | 0.0062 | 0.0037 | 0.0035 |
None | 0.2286 | 0.1415 | 0.168 | 0.1898 | 0.235 | 0.1095 | 0.1577 | 0.1195 | 0.1106 | 0.1176 | 0.1207 | 0.0889 | 0.1261 | 0.1652 | 0.1219 | 0.0976 | 0.0756 | 0.1023 |
Nuclear Periphery | 0.0058 | 0.0035 | 0.0041 | 0.0035 | 0.0037 | 0.0049 | 0.0121 | 0.0013 | 0.0024 | 0.0023 | 0.0167 | 0.0035 | 0.0176 | 0.0016 | 0.0074 | 0.0137 | 0.0102 | 0.004 |
Nucleolus | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0009 | 0.0004 | 0.0014 | 0.0002 | 0.0005 | 0.0001 | 0.0019 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0009 | 0.0021 | 0.0009 |
Nucleus | 0.0121 | 0.0294 | 0.0228 | 0.0234 | 0.0453 | 0.0257 | 0.0079 | 0.0137 | 0.0119 | 0.015 | 0.0066 | 0.0225 | 0.0113 | 0.0037 | 0.0099 | 0.0142 | 0.0258 | 0.012 |
Peroxisomes | 0.0215 | 0.0004 | 0.0035 | 0.0162 | 0.0006 | 0.0034 | 0.0226 | 0.0003 | 0.0018 | 0.0002 | 0.0219 | 0.001 | 0.0005 | 0.0001 | 0.0013 | 0.0105 | 0.0014 | 0.0003 |
Punctate Nuclear | 0.0058 | 0.0041 | 0.0066 | 0.0032 | 0.0133 | 0.0142 | 0.0134 | 0.0042 | 0.007 | 0.002 | 0.0653 | 0.0127 | 0.0054 | 0.0011 | 0.012 | 0.0297 | 0.0356 | 0.003 |
Vacuole | 0.0019 | 0.0017 | 0.0019 | 0.0029 | 0.0014 | 0.0014 | 0.0046 | 0.0009 | 0.0008 | 0.0007 | 0.0015 | 0.0008 | 0.0024 | 0.0008 | 0.0019 | 0.0038 | 0.0025 | 0.0016 |
Vacuole Periphery | 0.0006 | 0.0003 | 0.0008 | 0.0004 | 0.0003 | 0.0004 | 0.0031 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0.0022 | 0.0001 | 0.0006 | 0.0014 | 0.0011 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 27.1803 | 11.3733 | 19.1807 | 21.1459 | 21.7441 | 21.9634 | 16.4653 | 22.2259 | 21.5461 | 21.8908 |
Translational Efficiency | 0.6352 | 1.041 | 0.6154 | 0.4937 | 0.64 | 0.9084 | 0.968 | 0.5566 | 0.578 | 0.6826 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1314 | 544 | 1861 | 1745 | 2099 | 1999 | 1525 | 1334 | 3413 | 2543 | 3386 | 3079 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 873.85 | 993.99 | 954.98 | 1032.57 | 867.30 | 935.09 | 1043.32 | 1072.45 | 869.82 | 947.69 | 994.77 | 1049.85 |
Standard Deviation | 122.90 | 137.10 | 172.05 | 137.14 | 110.89 | 121.92 | 127.63 | 133.93 | 115.70 | 127.63 | 159.80 | 137.19 |
Intensity Change Log 2 | — | 0.185846 | 0.128085 | 0.240782 | — | 0.108574 | 0.266579 | 0.306307 | — | 0.147872 | 0.198733 | 0.273793 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000120 | 0.000288 | 0.000201 | 0.001630 | 0.000082 | 0.000226 | 0.000224 | 0.002846 | 0.000097 | 0.000239 | 0.000211 | 0.002157 |
Bud Neck | 0.001429 | 0.001600 | 0.003021 | 0.004723 | 0.001702 | 0.003269 | 0.001336 | 0.005186 | 0.001597 | 0.002912 | 0.002262 | 0.004924 |
Bud Site | 0.003016 | 0.007453 | 0.004505 | 0.027547 | 0.002349 | 0.005878 | 0.003738 | 0.023733 | 0.002606 | 0.006215 | 0.004159 | 0.025894 |
Cell Periphery | 0.000087 | 0.000259 | 0.000075 | 0.000128 | 0.000068 | 0.000055 | 0.000125 | 0.000096 | 0.000075 | 0.000099 | 0.000098 | 0.000114 |
Cytoplasm | 0.745838* | 0.727091* | 0.541411* | 0.568457* | 0.725326* | 0.743014* | 0.574449* | 0.512608* | 0.733223* | 0.739607* | 0.556291* | 0.544260* |
Cytoplasmic Foci | 0.066834 | 0.059975 | 0.003646 | 0.013230 | 0.067815 | 0.061336 | 0.013333 | 0.026781 | 0.067437 | 0.061044 | 0.008009 | 0.019101 |
Eisosomes | 0.000025 | 0.000029 | 0.000010 | 0.000020 | 0.000021 | 0.000018 | 0.000035 | 0.000024 | 0.000022 | 0.000021 | 0.000021 | 0.000022 |
Endoplasmic Reticulum | 0.001009 | 0.001239 | 0.001608 | 0.001564 | 0.001046 | 0.000683 | 0.002065 | 0.001152 | 0.001032 | 0.000802 | 0.001814 | 0.001385 |
Endosome | 0.002797 | 0.005530 | 0.000608 | 0.008647 | 0.002304 | 0.003563 | 0.002135 | 0.018509 | 0.002494 | 0.003984 | 0.001296 | 0.012920 |
Golgi | 0.000330 | 0.001856 | 0.000087 | 0.010454 | 0.000548 | 0.001367 | 0.000095 | 0.013773 | 0.000464 | 0.001472 | 0.000091 | 0.011892 |
Lipid Particles | 0.000998 | 0.002191 | 0.000359 | 0.000901 | 0.001125 | 0.000904 | 0.000989 | 0.001222 | 0.001076 | 0.001180 | 0.000642 | 0.001040 |
Mitochondria | 0.000628 | 0.001122 | 0.000456 | 0.003531 | 0.000954 | 0.002171 | 0.000299 | 0.002542 | 0.000828 | 0.001947 | 0.000385 | 0.003103 |
Mitotic Spindle | 0.000397 | 0.006164 | 0.004018 | 0.029347 | 0.001688 | 0.007837 | 0.002633 | 0.124847* | 0.001191 | 0.007479 | 0.003394 | 0.070723 |
None | 0.008143 | 0.004499 | 0.008433 | 0.006715 | 0.003971 | 0.003228 | 0.008362 | 0.005477 | 0.005577 | 0.003500 | 0.008401 | 0.006179 |
Nuclear Periphery | 0.000226 | 0.000245 | 0.000793 | 0.000726 | 0.000218 | 0.000211 | 0.000846 | 0.001510 | 0.000221 | 0.000219 | 0.000817 | 0.001066 |
Nuclear Periphery Foci | 0.002107 | 0.004237 | 0.001233 | 0.001291 | 0.001267 | 0.001327 | 0.002733 | 0.002445 | 0.001591 | 0.001950 | 0.001908 | 0.001791 |
Nucleolus | 0.000374 | 0.000445 | 0.000151 | 0.000177 | 0.000287 | 0.000306 | 0.000308 | 0.000304 | 0.000320 | 0.000336 | 0.000222 | 0.000232 |
Nucleus | 0.116422 | 0.113946 | 0.399353* | 0.226927* | 0.155183* | 0.112355 | 0.297391* | 0.104547 | 0.140260* | 0.112696 | 0.353431* | 0.173905* |
Peroxisomes | 0.000394 | 0.001103 | 0.000152 | 0.001569 | 0.000402 | 0.001619 | 0.000317 | 0.001178 | 0.000399 | 0.001509 | 0.000226 | 0.001400 |
Vacuole | 0.048363 | 0.060198 | 0.029684 | 0.086238 | 0.033337 | 0.049944 | 0.088463 | 0.137693 | 0.039122 | 0.052138 | 0.056157 | 0.108531 |
Vacuole Periphery | 0.000463 | 0.000528 | 0.000195 | 0.006178 | 0.000308 | 0.000689 | 0.000125 | 0.013528 | 0.000368 | 0.000655 | 0.000163 | 0.009362 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.71 | -5.44 | -3.83 | -2.58 | -2.77 | -4.13 | -6.59 | -1.88 | -1.46 | -1.35 | -4.86 | -9.00 | -3.54 | -2.70 | -2.52 |
Bud Neck | -0.81 | -3.25 | -5.25 | -4.73 | -0.58 | -3.71 | -0.75 | -5.19 | -0.85 | -6.22 | -4.10 | -3.17 | -6.96 | -2.38 | -2.44 |
Bud Site | -3.03 | -1.67 | -5.75 | -1.68 | -4.26 | -4.15 | -1.77 | -6.62 | -3.27 | -5.39 | -5.00 | -2.56 | -8.55 | -4.05 | -6.47 |
Cell Periphery | -1.60 | 0.81 | -1.70 | 1.48 | -2.38 | 3.08 | -8.42 | -2.27 | -5.60 | 6.54 | -0.96 | -5.11 | -3.46 | 0.27 | 1.54 |
Cytoplasm | 2.90 | 32.11 | 36.56 | 22.17 | 4.05 | -0.98 | 30.13 | 35.53 | 34.96 | 6.66 | 0.84 | 44.48 | 51.64 | 45.49 | 7.49 |
Cytoplasmic Foci | 1.39 | 23.96 | 22.32 | 13.24 | -5.92 | 1.84 | 24.02 | 22.90 | 21.04 | -0.76 | 2.27 | 34.37 | 32.45 | 26.23 | -3.82 |
Eisosomes | -1.62 | 6.99 | -1.58 | 0.83 | -10.15 | 1.32 | -11.19 | -8.50 | -9.77 | 4.84 | 0.85 | -3.88 | -7.03 | -7.04 | -2.57 |
Endoplasmic Reticulum | -1.42 | -6.53 | -4.07 | -2.22 | 3.74 | 2.52 | -9.46 | -2.32 | -8.15 | 9.72 | 1.89 | -11.01 | -4.68 | -9.47 | 8.84 |
Endosome | -2.85 | 8.67 | 1.72 | 3.53 | -3.01 | -2.85 | 1.39 | -2.27 | -0.51 | -2.82 | -3.70 | 7.64 | -0.67 | 2.18 | -4.03 |
Golgi | -1.60 | 2.82 | -2.18 | -0.15 | -2.56 | -2.30 | 3.00 | -2.55 | -1.41 | -3.29 | -3.01 | 3.77 | -3.18 | -1.20 | -4.03 |
Lipid Particles | -1.88 | 4.15 | -2.13 | 1.46 | -10.86 | 0.94 | 0.20 | -0.21 | -1.56 | -0.84 | -0.43 | 2.41 | -1.13 | -0.30 | -7.88 |
Mitochondria | -0.55 | 0.11 | -1.18 | -0.18 | -2.20 | -1.25 | 1.48 | 0.30 | 1.58 | -3.70 | -1.47 | 1.22 | -0.51 | 1.32 | -3.47 |
Mitotic Spindle | -2.35 | -4.56 | -8.51 | -3.31 | -4.97 | -3.60 | -0.97 | -8.55 | -6.68 | -8.35 | -4.51 | -4.04 | -11.69 | -7.52 | -9.64 |
None | 2.81 | 0.49 | 1.22 | -4.20 | 2.11 | 2.23 | -3.28 | -4.21 | -6.29 | 0.57 | 3.85 | -2.30 | -1.32 | -9.19 | 1.62 |
Nuclear Periphery | -1.27 | -18.06 | -22.31 | -23.51 | -7.80 | -0.62 | -26.12 | -16.38 | -16.26 | -6.11 | -0.99 | -30.60 | -27.74 | -27.81 | -9.77 |
Nuclear Periphery Foci | -3.34 | 3.31 | 3.54 | 4.50 | -0.22 | -0.41 | -8.14 | -3.35 | -2.41 | 5.10 | -1.96 | -3.38 | 0.15 | 2.15 | 3.94 |
Nucleolus | -0.59 | 3.68 | 2.07 | 1.74 | -4.17 | -0.45 | -0.13 | -0.36 | 0.21 | -0.42 | -0.49 | 2.09 | 0.92 | 1.57 | -2.62 |
Nucleus | 0.25 | -46.83 | -26.11 | -21.49 | 21.90 | 9.53 | -27.27 | -7.41 | -14.62 | 17.67 | 7.44 | -51.39 | -23.18 | -28.99 | 27.40 |
Peroxisomes | -1.52 | 3.46 | 0.22 | 1.53 | -1.45 | -2.84 | 1.74 | 1.09 | 3.18 | -0.92 | -3.17 | 3.15 | 0.67 | 3.33 | -1.68 |
Vacuole | -3.79 | 0.19 | -27.09 | -17.73 | -27.62 | -9.34 | -20.28 | -34.39 | -30.24 | -17.60 | -8.34 | -16.31 | -44.45 | -37.57 | -30.66 |
Vacuole Periphery | -0.15 | 1.34 | -1.42 | -1.46 | -2.28 | -1.44 | 3.03 | -2.54 | -2.02 | -2.80 | -1.24 | 2.34 | -2.99 | -2.24 | -3.61 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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