Standard name
Human Ortholog
Description Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.95 0.85 0.77 0.79 0.76 0.73 0.62 0.64 0.98 0.99 0.98 0.93 0.9 0.88 0.95 0.93 0.89 0.95 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.23 0.16 0.23 0.21 0.41 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.05 0.07 0.09 0.07 0.09 0.05 0.13 0.1 0.19 0 0 0 0.09 0.13 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 2 3 8 10 1 0 1 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 2 3 0 1 0 0 0 2 1 3 8 5 8 0 0 0 0 0 0
Cytoplasm 191 204 140 167 312 221 231 282 185 201 183 173 382 576 544 136 144 128 179 191 143 175 382 459
Endoplasmic Reticulum 1 2 0 0 1 3 3 0 4 0 1 0 1 0 2 2 2 3 0 0 1 0 1 1
Endosome 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 2 3 0 0 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0
Mitochondria 3 2 0 2 3 28 68 57 56 59 121 78 0 0 0 0 4 2 0 3 2 0 1 0
Nucleus 0 1 1 5 2 4 12 10 13 9 17 16 3 5 2 1 0 3 0 5 2 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 7 8 22 24 22 33 12 37 31 51 10 12 18 13 21 10 0 1 2 1 1 3
Unique Cell Count 193 206 144 172 327 259 299 359 244 277 296 269 389 582 555 146 160 145 189 206 160 185 395 477
Labelled Cell Count 195 209 148 183 342 282 342 387 275 316 364 319 398 595 569 162 178 158 189 206 160 185 395 477


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.7 7.6 7.3 7.3 6.1 5.6 6.1 5.3 5.8 5.0 5.4 7.6 8.0 8.0 12.1 11.9 11.9 7.8 8.5 8.9
Std Deviation (1e-4) 0.7 1.1 1.0 0.8 1.7 1.3 1.2 1.2 1.4 1.5 1.1 0.8 0.9 0.9 0.9 2.3 1.9 2.2 1.2 1.3 1.4
Intensity Change (Log2) – – – -0.05 -0.06 -0.31 -0.44 -0.33 -0.52 -0.39 -0.6 -0.49 0.0 0.08 0.08 0.67 0.64 0.64 0.03 0.16 0.23

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.9 -3.7 -5.3 -5.1 -5.5 -6.1 -7.9 -7.4 0.7 1.6 0.6 -1.6 -2.5 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 6.2 5.1 6.2 6.0 9.0 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2.4 0 2.5 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 0.8 1.6 1.0 1.6 0 2.7 2.0 3.9 0 0 -0.9 1.4 2.5 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2027 1.9543 1.7884 1.4478 1.1279 1.6921 2.2925 2.9235 2.5722 1.6498 2.7579 2.4716 2.1076 2.7938 2.4006 2.5615 2.4073 2.1161
Actin 0.014 0.0011 0.0104 0.0138 0.0015 0.008 0.023 0.0012 0.0139 0.0012 0.0523 0.0084 0.0319 0.0009 0.0335 0.0364 0.0437 0.0044
Bud 0.0006 0.0004 0.0004 0.0005 0.0026 0.0006 0.002 0.0037 0.0031 0.0001 0.0036 0.0006 0.0044 0.0005 0.0044 0.0063 0.0348 0.0054
Bud Neck 0.002 0.0004 0.0009 0.0007 0.0007 0.0024 0.0097 0.0004 0.0012 0.0005 0.0017 0.0149 0.0017 0.0004 0.005 0.0014 0.0012 0.0013
Bud Periphery 0.0006 0.0002 0.0011 0.0003 0.0012 0.0006 0.0019 0.0011 0.0022 0 0.0043 0.0004 0.005 0.0004 0.0015 0.0038 0.0114 0.0021
Bud Site 0.0028 0.0017 0.0007 0.0008 0.0056 0.0006 0.0085 0.0057 0.0086 0.0003 0.0047 0.0003 0.0041 0.004 0.0083 0.0119 0.0041 0.0003
Cell Periphery 0.0001 0 0.0001 0 0.0001 0 0.0004 0.0001 0.0002 0.0001 0.0002 0 0.0012 0.0002 0.0005 0.0005 0.0005 0.0002
Cytoplasm 0.6262 0.7955 0.7519 0.7192 0.667 0.781 0.6033 0.8385 0.8172 0.8446 0.544 0.8122 0.7362 0.8117 0.7424 0.6733 0.6887 0.8193
Cytoplasmic Foci 0.0292 0.0101 0.0122 0.0144 0.0122 0.0253 0.0546 0.005 0.0115 0.0064 0.0413 0.0167 0.0223 0.003 0.0151 0.0469 0.0142 0.0204
Eisosomes 0.0002 0 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0.0004 0 0.0002 0 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0046 0.0027 0.0031 0.0028 0.0028 0.0038 0.0067 0.0024 0.0025 0.0047 0.0055 0.0034 0.0084 0.0048 0.0092 0.0071 0.0164 0.0036
Endosome 0.0217 0.0058 0.0058 0.0052 0.005 0.0137 0.0381 0.0012 0.0024 0.0032 0.0354 0.0099 0.0134 0.0011 0.014 0.0192 0.0157 0.0113
Golgi 0.0051 0.0003 0.0013 0.0012 0.0003 0.0014 0.009 0.0002 0.0007 0.0003 0.0188 0.0015 0.0015 0.0001 0.0057 0.0094 0.0068 0.0022
Lipid Particles 0.0083 0.0001 0.001 0.0003 0.0002 0.001 0.0108 0.0001 0.0005 0.0003 0.0467 0.0011 0.0008 0.0001 0.0012 0.0055 0.0041 0.001
Mitochondria 0.0079 0.0005 0.0028 0.0011 0.0005 0.0017 0.0092 0.0003 0.0008 0.0003 0.0053 0.0006 0.0021 0.0002 0.0037 0.0062 0.0037 0.0035
None 0.2286 0.1415 0.168 0.1898 0.235 0.1095 0.1577 0.1195 0.1106 0.1176 0.1207 0.0889 0.1261 0.1652 0.1219 0.0976 0.0756 0.1023
Nuclear Periphery 0.0058 0.0035 0.0041 0.0035 0.0037 0.0049 0.0121 0.0013 0.0024 0.0023 0.0167 0.0035 0.0176 0.0016 0.0074 0.0137 0.0102 0.004
Nucleolus 0.0004 0.0002 0.0004 0.0003 0.0009 0.0004 0.0014 0.0002 0.0005 0.0001 0.0019 0.0002 0.0013 0.0001 0.0005 0.0009 0.0021 0.0009
Nucleus 0.0121 0.0294 0.0228 0.0234 0.0453 0.0257 0.0079 0.0137 0.0119 0.015 0.0066 0.0225 0.0113 0.0037 0.0099 0.0142 0.0258 0.012
Peroxisomes 0.0215 0.0004 0.0035 0.0162 0.0006 0.0034 0.0226 0.0003 0.0018 0.0002 0.0219 0.001 0.0005 0.0001 0.0013 0.0105 0.0014 0.0003
Punctate Nuclear 0.0058 0.0041 0.0066 0.0032 0.0133 0.0142 0.0134 0.0042 0.007 0.002 0.0653 0.0127 0.0054 0.0011 0.012 0.0297 0.0356 0.003
Vacuole 0.0019 0.0017 0.0019 0.0029 0.0014 0.0014 0.0046 0.0009 0.0008 0.0007 0.0015 0.0008 0.0024 0.0008 0.0019 0.0038 0.0025 0.0016
Vacuole Periphery 0.0006 0.0003 0.0008 0.0004 0.0003 0.0004 0.0031 0.0001 0.0002 0.0001 0.0012 0.0002 0.0022 0.0001 0.0006 0.0014 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.1803 11.3733 19.1807 21.1459 21.7441 21.9634 16.4653 22.2259 21.5461 21.8908
Translational Efficiency 0.6352 1.041 0.6154 0.4937 0.64 0.9084 0.968 0.5566 0.578 0.6826

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1314 544 1861 1745 2099 1999 1525 1334 3413 2543 3386 3079

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 873.85 993.99 954.98 1032.57 867.30 935.09 1043.32 1072.45 869.82 947.69 994.77 1049.85
Standard Deviation 122.90 137.10 172.05 137.14 110.89 121.92 127.63 133.93 115.70 127.63 159.80 137.19
Intensity Change Log 2 — 0.185846 0.128085 0.240782 — 0.108574 0.266579 0.306307 — 0.147872 0.198733 0.273793

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000120 0.000288 0.000201 0.001630 0.000082 0.000226 0.000224 0.002846 0.000097 0.000239 0.000211 0.002157
Bud Neck 0.001429 0.001600 0.003021 0.004723 0.001702 0.003269 0.001336 0.005186 0.001597 0.002912 0.002262 0.004924
Bud Site 0.003016 0.007453 0.004505 0.027547 0.002349 0.005878 0.003738 0.023733 0.002606 0.006215 0.004159 0.025894
Cell Periphery 0.000087 0.000259 0.000075 0.000128 0.000068 0.000055 0.000125 0.000096 0.000075 0.000099 0.000098 0.000114
Cytoplasm 0.745838 0.727091 0.541411 0.568457 0.725326 0.743014 0.574449 0.512608 0.733223 0.739607 0.556291 0.544260
Cytoplasmic Foci 0.066834 0.059975 0.003646 0.013230 0.067815 0.061336 0.013333 0.026781 0.067437 0.061044 0.008009 0.019101
Eisosomes 0.000025 0.000029 0.000010 0.000020 0.000021 0.000018 0.000035 0.000024 0.000022 0.000021 0.000021 0.000022
Endoplasmic Reticulum 0.001009 0.001239 0.001608 0.001564 0.001046 0.000683 0.002065 0.001152 0.001032 0.000802 0.001814 0.001385
Endosome 0.002797 0.005530 0.000608 0.008647 0.002304 0.003563 0.002135 0.018509 0.002494 0.003984 0.001296 0.012920
Golgi 0.000330 0.001856 0.000087 0.010454 0.000548 0.001367 0.000095 0.013773 0.000464 0.001472 0.000091 0.011892
Lipid Particles 0.000998 0.002191 0.000359 0.000901 0.001125 0.000904 0.000989 0.001222 0.001076 0.001180 0.000642 0.001040
Mitochondria 0.000628 0.001122 0.000456 0.003531 0.000954 0.002171 0.000299 0.002542 0.000828 0.001947 0.000385 0.003103
Mitotic Spindle 0.000397 0.006164 0.004018 0.029347 0.001688 0.007837 0.002633 0.124847 0.001191 0.007479 0.003394 0.070723
None 0.008143 0.004499 0.008433 0.006715 0.003971 0.003228 0.008362 0.005477 0.005577 0.003500 0.008401 0.006179
Nuclear Periphery 0.000226 0.000245 0.000793 0.000726 0.000218 0.000211 0.000846 0.001510 0.000221 0.000219 0.000817 0.001066
Nuclear Periphery Foci 0.002107 0.004237 0.001233 0.001291 0.001267 0.001327 0.002733 0.002445 0.001591 0.001950 0.001908 0.001791
Nucleolus 0.000374 0.000445 0.000151 0.000177 0.000287 0.000306 0.000308 0.000304 0.000320 0.000336 0.000222 0.000232
Nucleus 0.116422 0.113946 0.399353 0.226927 0.155183 0.112355 0.297391 0.104547 0.140260 0.112696 0.353431 0.173905
Peroxisomes 0.000394 0.001103 0.000152 0.001569 0.000402 0.001619 0.000317 0.001178 0.000399 0.001509 0.000226 0.001400
Vacuole 0.048363 0.060198 0.029684 0.086238 0.033337 0.049944 0.088463 0.137693 0.039122 0.052138 0.056157 0.108531
Vacuole Periphery 0.000463 0.000528 0.000195 0.006178 0.000308 0.000689 0.000125 0.013528 0.000368 0.000655 0.000163 0.009362

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.71 -5.44 -3.83 -2.58 -2.77 -4.13 -6.59 -1.88 -1.46 -1.35 -4.86 -9.00 -3.54 -2.70 -2.52
Bud Neck -0.81 -3.25 -5.25 -4.73 -0.58 -3.71 -0.75 -5.19 -0.85 -6.22 -4.10 -3.17 -6.96 -2.38 -2.44
Bud Site -3.03 -1.67 -5.75 -1.68 -4.26 -4.15 -1.77 -6.62 -3.27 -5.39 -5.00 -2.56 -8.55 -4.05 -6.47
Cell Periphery -1.60 0.81 -1.70 1.48 -2.38 3.08 -8.42 -2.27 -5.60 6.54 -0.96 -5.11 -3.46 0.27 1.54
Cytoplasm 2.90 32.11 36.56 22.17 4.05 -0.98 30.13 35.53 34.96 6.66 0.84 44.48 51.64 45.49 7.49
Cytoplasmic Foci 1.39 23.96 22.32 13.24 -5.92 1.84 24.02 22.90 21.04 -0.76 2.27 34.37 32.45 26.23 -3.82
Eisosomes -1.62 6.99 -1.58 0.83 -10.15 1.32 -11.19 -8.50 -9.77 4.84 0.85 -3.88 -7.03 -7.04 -2.57
Endoplasmic Reticulum -1.42 -6.53 -4.07 -2.22 3.74 2.52 -9.46 -2.32 -8.15 9.72 1.89 -11.01 -4.68 -9.47 8.84
Endosome -2.85 8.67 1.72 3.53 -3.01 -2.85 1.39 -2.27 -0.51 -2.82 -3.70 7.64 -0.67 2.18 -4.03
Golgi -1.60 2.82 -2.18 -0.15 -2.56 -2.30 3.00 -2.55 -1.41 -3.29 -3.01 3.77 -3.18 -1.20 -4.03
Lipid Particles -1.88 4.15 -2.13 1.46 -10.86 0.94 0.20 -0.21 -1.56 -0.84 -0.43 2.41 -1.13 -0.30 -7.88
Mitochondria -0.55 0.11 -1.18 -0.18 -2.20 -1.25 1.48 0.30 1.58 -3.70 -1.47 1.22 -0.51 1.32 -3.47
Mitotic Spindle -2.35 -4.56 -8.51 -3.31 -4.97 -3.60 -0.97 -8.55 -6.68 -8.35 -4.51 -4.04 -11.69 -7.52 -9.64
None 2.81 0.49 1.22 -4.20 2.11 2.23 -3.28 -4.21 -6.29 0.57 3.85 -2.30 -1.32 -9.19 1.62
Nuclear Periphery -1.27 -18.06 -22.31 -23.51 -7.80 -0.62 -26.12 -16.38 -16.26 -6.11 -0.99 -30.60 -27.74 -27.81 -9.77
Nuclear Periphery Foci -3.34 3.31 3.54 4.50 -0.22 -0.41 -8.14 -3.35 -2.41 5.10 -1.96 -3.38 0.15 2.15 3.94
Nucleolus -0.59 3.68 2.07 1.74 -4.17 -0.45 -0.13 -0.36 0.21 -0.42 -0.49 2.09 0.92 1.57 -2.62
Nucleus 0.25 -46.83 -26.11 -21.49 21.90 9.53 -27.27 -7.41 -14.62 17.67 7.44 -51.39 -23.18 -28.99 27.40
Peroxisomes -1.52 3.46 0.22 1.53 -1.45 -2.84 1.74 1.09 3.18 -0.92 -3.17 3.15 0.67 3.33 -1.68
Vacuole -3.79 0.19 -27.09 -17.73 -27.62 -9.34 -20.28 -34.39 -30.24 -17.60 -8.34 -16.31 -44.45 -37.57 -30.66
Vacuole Periphery -0.15 1.34 -1.42 -1.46 -2.28 -1.44 3.03 -2.54 -2.02 -2.80 -1.24 2.34 -2.99 -2.24 -3.61
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bud17

Bud17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available