ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 10 | 47 | 33 | 11 | 17 | – | 3 | 7 | 8 | 2 | 8 | 77 | 50 | 73 | – | 2 | – | 14 | 15 | 42 | 7 | 32 | 40 |
Bud | 1 | 2 | 5 | 9 | 0 | 0 | – | 3 | 2 | 0 | 2 | 7 | 26 | 20 | 34 | – | 0 | – | 5 | 4 | 2 | 2 | 8 | 24 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | 0 | – | 5 | 8 | 4 | 1 | 4 | 6 |
Bud Site | 2 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 12 | 11 | 6 | 8 | – | 1 | 8 | 7 | 4 | 3 | 33 | 33 | 41 | – | 0 | – | 1 | 0 | 5 | 2 | 4 | 1 |
Cytoplasm | 8 | 8 | 12 | 26 | 35 | 56 | – | 99 | 152 | 151 | 161 | 161 | 54 | 126 | 187 | – | 0 | – | 0 | 3 | 1 | 1 | 1 | 2 |
Endoplasmic Reticulum | 30 | 20 | 52 | 46 | 76 | 64 | – | 38 | 37 | 25 | 27 | 23 | 113 | 58 | 29 | – | 1 | – | 18 | 7 | 32 | 10 | 19 | 27 |
Endosome | 0 | 1 | 2 | 3 | 0 | 0 | – | 1 | 3 | 2 | 0 | 4 | 7 | 2 | 1 | – | 0 | – | 0 | 7 | 1 | 0 | 2 | 5 |
Golgi | 7 | 0 | 6 | 8 | 21 | 4 | – | 6 | 2 | 0 | 1 | 5 | 3 | 0 | 0 | – | 1 | – | 5 | 1 | 5 | 1 | 2 | 4 |
Mitochondria | 6 | 8 | 9 | 4 | 10 | 4 | – | 17 | 19 | 19 | 26 | 10 | 2 | 0 | 0 | – | 0 | – | 4 | 4 | 7 | 6 | 13 | 8 |
Nucleus | 18 | 4 | 14 | 19 | 9 | 39 | – | 145 | 110 | 97 | 62 | 66 | 17 | 17 | 8 | – | 3 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 1 | 0 | 2 | 2 | – | 2 | 7 | 1 | 0 | 2 | 0 | 1 | 0 | – | 0 | – | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 3 | – | 3 | 4 | 4 | 4 | 5 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 5 | 2 | 0 | 1 | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 12 | – | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 77 | 16 | 114 | 101 | 206 | 225 | – | 501 | 395 | 378 | 321 | 348 | 90 | 50 | 33 | – | 14 | – | 103 | 57 | 171 | 30 | 46 | 53 |
Unique Cell Count | 138 | 65 | 228 | 213 | 329 | 370 | 704 | 601 | 581 | 501 | 532 | 345 | 308 | 344 | 19 | 160 | 115 | 278 | 66 | 139 | 179 | |||
Labelled Cell Count | 177 | 74 | 282 | 264 | 377 | 423 | 820 | 746 | 692 | 610 | 644 | 424 | 366 | 425 | 22 | 160 | 115 | 278 | 66 | 139 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.5 | 7.6 | 11.2 | 12.4 | 16.5 | 15.8 | – | 15.5 | 15.6 | 14.7 | 14.3 | 14.6 | 8.7 | 7.4 | 6.8 | – | 10.8 | – | 12.3 | 12.3 | 12.2 |
Std Deviation (1e-4) | 3.2 | 2.2 | 3.1 | 2.9 | 3.6 | 3.0 | – | 3.5 | 3.5 | 3.5 | 3.5 | 3.6 | 1.6 | 1.5 | 1.3 | – | 1.8 | – | 3.1 | 2.8 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.15 | 0.56 | 0.5 | – | 0.47 | 0.47 | 0.39 | 0.35 | 0.38 | -0.36 | -0.6 | -0.71 | – | -0.05 | – | 0.14 | 0.13 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -6.6 | -6.2 | – | -11.8 | -10.1 | -9.8 | -10.1 | -9.3 | 0.5 | -1.3 | 0.2 | – | 0 | – |
Bud | 1.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.3 | 3.6 | – | 0 | – |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Cell Periphery | 0 | -2.3 | -2.0 | – | 0 | -3.3 | -3.4 | -3.8 | 0 | 1.9 | 2.2 | 2.7 | – | 0 | – |
Cytoplasm | 2.6 | 2.2 | 3.7 | – | 3.6 | 6.5 | 6.6 | 7.9 | 7.5 | 3.8 | 9.3 | 12.1 | – | 0 | – |
Endoplasmic Reticulum | -0.3 | 0.1 | -1.7 | – | -7.7 | -6.9 | -8.1 | -7.0 | -7.8 | 2.6 | -1.1 | -4.8 | – | 0 | – |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Golgi | 0.7 | 2.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Mitochondria | -1.3 | -0.6 | 0 | – | -1.2 | -0.6 | -0.5 | 0.7 | -1.7 | 0 | 0 | 0 | – | 0 | – |
Nucleus | 1.1 | -2.0 | 1.8 | – | 5.0 | 4.4 | 3.9 | 2.6 | 2.6 | -0.6 | -0.3 | -2.3 | – | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | – | 0 | – |
Vacuole | -0.5 | 3.0 | 2.6 | – | 5.9 | 4.2 | 3.9 | 3.6 | 4.0 | -5.9 | -8.4 | -10.8 | – | 2.0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5426 | 3.1057 | 3.1627 | 2.5457 | 1.7561 | 3.2813 | 3.4632 | 3.9292 | 3.3494 | 3.2312 | 2.4931 | 3.521 | 2.8986 | 3.4159 | 3.0204 | 2.8864 | 2.9919 | 3.4075 |
Actin | 0.0297 | 0.005 | 0.0196 | 0.0073 | 0.0009 | 0.0094 | 0.0637 | 0.0321 | 0.0471 | 0.0672 | 0.0011 | 0.018 | 0.0761 | 0.0109 | 0.0585 | 0.0277 | 0.0116 | 0.0141 |
Bud | 0.0318 | 0.0176 | 0.0722 | 0.018 | 0.0259 | 0.0039 | 0.0107 | 0.0081 | 0.0345 | 0.0369 | 0.0052 | 0.0078 | 0.0157 | 0.0202 | 0.0579 | 0.0191 | 0.0333 | 0.0059 |
Bud Neck | 0.0274 | 0.0119 | 0.013 | 0.0055 | 0.0245 | 0.2664 | 0.0546 | 0.019 | 0.0142 | 0.0272 | 0.06 | 0.2893 | 0.0385 | 0.0299 | 0.0295 | 0.0049 | 0.0209 | 0.3655 |
Bud Periphery | 0.1144 | 0.0463 | 0.2673 | 0.1201 | 0.0952 | 0.0197 | 0.0384 | 0.0216 | 0.1726 | 0.3324 | 0.0285 | 0.0332 | 0.0694 | 0.0599 | 0.2005 | 0.1702 | 0.1551 | 0.0238 |
Bud Site | 0.0566 | 0.1626 | 0.0374 | 0.0198 | 0.0199 | 0.0059 | 0.0892 | 0.2585 | 0.0636 | 0.0104 | 0.0068 | 0.0092 | 0.0611 | 0.1446 | 0.0536 | 0.0059 | 0.0164 | 0.0056 |
Cell Periphery | 0.0418 | 0.03 | 0.0218 | 0.0204 | 0.0076 | 0.0221 | 0.0641 | 0.0347 | 0.0236 | 0.0348 | 0.0222 | 0.0273 | 0.0478 | 0.0234 | 0.021 | 0.0221 | 0.0617 | 0.0279 |
Cytoplasm | 0.1595 | 0.0796 | 0.0746 | 0.1351 | 0.1937 | 0.1213 | 0.0862 | 0.1143 | 0.0766 | 0.014 | 0.1305 | 0.1066 | 0.1166 | 0.1092 | 0.087 | 0.1102 | 0.0789 | 0.0855 |
Cytoplasmic Foci | 0.0405 | 0.0283 | 0.0135 | 0.0093 | 0.0214 | 0.0225 | 0.0377 | 0.039 | 0.0299 | 0.004 | 0.0109 | 0.021 | 0.0461 | 0.0343 | 0.02 | 0.0146 | 0.0115 | 0.0206 |
Eisosomes | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0045 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0013 | 0.0002 | 0.0004 | 0.0002 | 0.0007 | 0.0001 |
Endoplasmic Reticulum | 0.0132 | 0.0144 | 0.0142 | 0.0214 | 0.0148 | 0.0109 | 0.0248 | 0.0091 | 0.0173 | 0.0204 | 0.013 | 0.0152 | 0.0176 | 0.0213 | 0.0109 | 0.0169 | 0.0382 | 0.0246 |
Endosome | 0.1609 | 0.135 | 0.0779 | 0.1766 | 0.1809 | 0.104 | 0.0934 | 0.0838 | 0.119 | 0.0825 | 0.1005 | 0.1073 | 0.1473 | 0.1374 | 0.1071 | 0.1378 | 0.1486 | 0.073 |
Golgi | 0.019 | 0.0177 | 0.0055 | 0.0122 | 0.0109 | 0.012 | 0.0311 | 0.0105 | 0.0229 | 0.0072 | 0.0055 | 0.0157 | 0.0204 | 0.0126 | 0.016 | 0.0078 | 0.0176 | 0.0096 |
Lipid Particles | 0.0167 | 0.0159 | 0.0035 | 0.0057 | 0.0034 | 0.005 | 0.0484 | 0.0081 | 0.018 | 0.0032 | 0.0125 | 0.0082 | 0.0324 | 0.0229 | 0.0053 | 0.0041 | 0.0177 | 0.008 |
Mitochondria | 0.0054 | 0.0061 | 0.0119 | 0.0084 | 0.006 | 0.0081 | 0.0118 | 0.0061 | 0.031 | 0.0199 | 0.0462 | 0.0065 | 0.0054 | 0.0064 | 0.0141 | 0.005 | 0.0092 | 0.0028 |
None | 0.0202 | 0.0517 | 0.0523 | 0.0018 | 0.0043 | 0.014 | 0.0456 | 0.0823 | 0.0734 | 0.0008 | 0.0277 | 0.0093 | 0.0244 | 0.0522 | 0.0647 | 0.0601 | 0.0015 | 0.0043 |
Nuclear Periphery | 0.0029 | 0.0025 | 0.0028 | 0.0052 | 0.0044 | 0.0021 | 0.0228 | 0.0028 | 0.0052 | 0.0044 | 0.0037 | 0.002 | 0.0051 | 0.0038 | 0.0029 | 0.0031 | 0.0056 | 0.0032 |
Nucleolus | 0.0016 | 0.0034 | 0.0023 | 0.0018 | 0.0022 | 0.0016 | 0.0048 | 0.002 | 0.0015 | 0.0052 | 0.0023 | 0.0013 | 0.0014 | 0.0022 | 0.0017 | 0.0014 | 0.0013 | 0.0012 |
Nucleus | 0.0028 | 0.0072 | 0.004 | 0.0054 | 0.0128 | 0.0038 | 0.0107 | 0.0037 | 0.0033 | 0.0071 | 0.007 | 0.0033 | 0.003 | 0.0047 | 0.0031 | 0.0033 | 0.0034 | 0.0031 |
Peroxisomes | 0.0014 | 0.0014 | 0.0009 | 0.0003 | 0.0012 | 0.0008 | 0.0087 | 0.0029 | 0.0027 | 0.0004 | 0.0027 | 0.0008 | 0.0028 | 0.002 | 0.0013 | 0.0004 | 0.0005 | 0.0006 |
Punctate Nuclear | 0.0006 | 0.0009 | 0.0006 | 0.0003 | 0.0008 | 0.001 | 0.0074 | 0.0008 | 0.0011 | 0.0002 | 0.0008 | 0.0005 | 0.002 | 0.001 | 0.0007 | 0.0003 | 0.0003 | 0.0004 |
Vacuole | 0.2385 | 0.3478 | 0.2717 | 0.3892 | 0.3537 | 0.3457 | 0.2257 | 0.2512 | 0.2163 | 0.2868 | 0.4788 | 0.3049 | 0.2499 | 0.2782 | 0.2178 | 0.3492 | 0.3454 | 0.3097 |
Vacuole Periphery | 0.0148 | 0.0145 | 0.0329 | 0.0364 | 0.0155 | 0.0195 | 0.0158 | 0.0092 | 0.0256 | 0.035 | 0.0338 | 0.0127 | 0.0157 | 0.0229 | 0.026 | 0.0356 | 0.0206 | 0.0104 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.9752 | 21.443 | 17.2376 | 53.0974 | 51.4018 | 35.2947 | 22.7349 | 15.4461 | 36.7068 | 64.6492 |
Translational Efficiency | 1.2398 | 1.4774 | 1.1829 | 0.8412 | 1.0008 | 1.434 | 0.8496 | 0.9949 | 1.0429 | 0.949 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
109 | 687 | 1877 | 1619 | 1410 | 1657 | 2456 | 1383 | 1519 | 2344 | 4333 | 3002 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 981.05 | 963.10 | 963.27 | 1019.65 | 938.72 | 959.25 | 1024.46 | 951.64 | 941.76 | 960.38 | 997.95 | 988.32 |
Standard Deviation | 134.84 | 165.36 | 125.97 | 154.57 | 201.55 | 163.49 | 134.33 | 142.53 | 197.82 | 164.05 | 134.24 | 152.95 |
Intensity Change Log 2 | — | -0.026641 | -0.026386 | 0.055675 | — | 0.031212 | 0.126097 | 0.019721 | — | 0.001938 | 0.050188 | 0.038207 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.023158 | 0.021818 | 0.011257 | 0.193503* | 0.005095 | 0.006025 | 0.239613* | 0.194882* | 0.006391 | 0.010654 | 0.140692* | 0.194138* |
Bud Neck | 0.094089 | 0.083436 | 0.087239 | 0.091556 | 0.060407 | 0.092352 | 0.145454 | 0.089203 | 0.062824 | 0.089739 | 0.120236 | 0.090472 |
Bud Site | 0.178536* | 0.258560* | 0.334219* | 0.344329* | 0.150896* | 0.142212* | 0.344075* | 0.343737* | 0.152880* | 0.176312* | 0.339806* | 0.344056* |
Cell Periphery | 0.020474 | 0.099889 | 0.003468 | 0.179148* | 0.133954* | 0.161773* | 0.103365* | 0.128640* | 0.125811* | 0.143636* | 0.060091 | 0.155879* |
Cytoplasm | 0.097813 | 0.082326 | 0.205410* | 0.081100 | 0.024951 | 0.021826 | 0.078932 | 0.122193* | 0.030179 | 0.039558 | 0.133721* | 0.100031* |
Cytoplasmic Foci | 0.042294 | 0.015783 | 0.021343 | 0.003991 | 0.006786 | 0.006387 | 0.003212 | 0.009959 | 0.009334 | 0.009140 | 0.011066 | 0.006740 |
Eisosomes | 0.003495 | 0.000772 | 0.000215 | 0.027679 | 0.002203 | 0.000491 | 0.019223 | 0.019611 | 0.002296 | 0.000573 | 0.010989 | 0.023962 |
Endoplasmic Reticulum | 0.009489 | 0.028901 | 0.008577 | 0.004618 | 0.050543 | 0.052984 | 0.011935 | 0.005582 | 0.047597 | 0.045925 | 0.010480 | 0.005062 |
Endosome | 0.064040 | 0.038916 | 0.013998 | 0.002534 | 0.038638 | 0.037378 | 0.002591 | 0.005391 | 0.040461 | 0.037829 | 0.007532 | 0.003850 |
Golgi | 0.019631 | 0.003430 | 0.000811 | 0.001829 | 0.005442 | 0.004110 | 0.000662 | 0.002101 | 0.006461 | 0.003910 | 0.000727 | 0.001954 |
Lipid Particles | 0.002543 | 0.001814 | 0.001960 | 0.001949 | 0.006029 | 0.002039 | 0.003082 | 0.001775 | 0.005779 | 0.001973 | 0.002596 | 0.001869 |
Mitochondria | 0.004762 | 0.004000 | 0.000910 | 0.002351 | 0.005706 | 0.007408 | 0.001305 | 0.001366 | 0.005639 | 0.006409 | 0.001134 | 0.001897 |
Mitotic Spindle | 0.010491 | 0.000193 | 0.000694 | 0.000837 | 0.000191 | 0.000222 | 0.000196 | 0.000745 | 0.000930 | 0.000213 | 0.000412 | 0.000795 |
None | 0.000044 | 0.001743 | 0.000282 | 0.000076 | 0.013264 | 0.000471 | 0.000064 | 0.000054 | 0.012315 | 0.000844 | 0.000158 | 0.000066 |
Nuclear Periphery | 0.000185 | 0.000074 | 0.000320 | 0.000054 | 0.000093 | 0.000145 | 0.000037 | 0.000034 | 0.000100 | 0.000124 | 0.000159 | 0.000045 |
Nuclear Periphery Foci | 0.001158 | 0.000490 | 0.001249 | 0.000307 | 0.001562 | 0.000483 | 0.000227 | 0.000305 | 0.001533 | 0.000485 | 0.000670 | 0.000306 |
Nucleolus | 0.000611 | 0.000546 | 0.000349 | 0.000020 | 0.000842 | 0.000669 | 0.000027 | 0.000025 | 0.000825 | 0.000633 | 0.000166 | 0.000022 |
Nucleus | 0.011397 | 0.002920 | 0.037284 | 0.008174 | 0.002437 | 0.002673 | 0.008327 | 0.008484 | 0.003080 | 0.002746 | 0.020871 | 0.008317 |
Peroxisomes | 0.002997 | 0.003125 | 0.003565 | 0.001889 | 0.006095 | 0.001807 | 0.003649 | 0.005194 | 0.005873 | 0.002193 | 0.003613 | 0.003412 |
Vacuole | 0.404074* | 0.336172* | 0.264863* | 0.053689 | 0.443290* | 0.437762* | 0.033869 | 0.060227 | 0.440476* | 0.407987* | 0.133932 | 0.056701 |
Vacuole Periphery | 0.008722 | 0.015092 | 0.001988 | 0.000367 | 0.041575 | 0.020784 | 0.000157 | 0.000493 | 0.039218 | 0.019115 | 0.000950 | 0.000425 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.08 | 1.27 | -15.61 | -22.70* | -27.66* | -0.78 | -41.45* | -26.49* | -26.33* | 5.62 | -2.80 | -35.52* | -38.19* | -36.81* | -8.12 |
Bud Neck | 0.07 | 0.33 | -0.14 | -0.62 | -1.78 | -6.23 | -15.99 | -4.80 | 1.24 | 10.07 | -5.99 | -12.36 | -5.66 | 0.79 | 7.36 |
Bud Site | -3.34 | -5.00 | -6.20 | -5.13 | -2.96 | 1.01 | -21.26 | -17.02 | -18.59 | 0.86 | -2.92 | -21.45 | -21.10 | -19.60 | -1.21 |
Cell Periphery | -7.97 | 2.12 | -15.58 | -7.18 | -25.60* | -3.31 | 4.82 | 1.36 | 4.56 | -3.02 | -2.45 | 11.45 | -3.12 | -0.63 | -17.86 |
Cytoplasm | 1.12 | -7.46 | 0.69 | -0.98 | 18.40 | 1.11 | -15.08 | -18.54 | -19.38 | -7.74 | -2.93 | -28.41 | -19.65 | -17.10 | 6.94 |
Cytoplasmic Foci | 3.57 | 2.81 | 5.25 | 5.02 | 13.26 | 0.31 | 3.70 | -2.86 | -3.04 | -5.56 | 0.09 | -2.28 | 1.69 | 1.65 | 4.85 |
Eisosomes | 1.08 | 1.34 | -8.45 | -15.65 | -16.07 | 3.92 | -15.02 | -10.79 | -12.41 | 0.08 | 3.90 | -11.56 | -17.24 | -20.03 | -9.53 |
Endoplasmic Reticulum | -4.38 | 0.06 | 1.14 | 8.40 | 4.50 | -0.60 | 12.38 | 14.80 | 16.54 | 4.53 | 0.49 | 13.16 | 15.38 | 18.90 | 6.39 |
Endosome | 1.85 | 4.48 | 5.56 | 11.27 | 11.10 | 0.44 | 16.99 | 14.86 | 14.36 | -2.65 | 0.83 | 15.58 | 17.19 | 18.97 | 5.29 |
Golgi | 1.99 | 2.37 | 2.22 | 2.14 | -1.44 | 1.43 | 8.16 | 4.70 | 2.49 | -3.02 | 2.63 | 7.50 | 5.21 | 3.06 | -2.75 |
Lipid Particles | 1.35 | 0.86 | 0.90 | -0.24 | 0.34 | 3.29 | 2.37 | 3.50 | 0.56 | 2.61 | 3.41 | 2.78 | 3.45 | 0.24 | 2.15 |
Mitochondria | 0.09 | 1.11 | 0.65 | 1.42 | -2.05 | -1.58 | 6.14 | 5.88 | 7.21 | -0.11 | -0.85 | 6.43 | 4.81 | 6.12 | -1.87 |
Mitotic Spindle | 1.11 | 1.03 | 1.01 | -1.98 | -0.29 | -0.79 | -0.43 | -1.36 | -1.29 | -1.25 | 1.07 | 0.59 | -0.06 | -2.29 | -1.29 |
None | -1.31 | -4.81 | -1.70 | 1.25 | 2.92 | 5.12 | 5.36 | 5.35 | 1.15 | -0.20 | 4.90 | 5.31 | 5.34 | 1.66 | 2.34 |
Nuclear Periphery | 2.94 | -3.59 | 2.58 | -0.94 | 7.25 | -2.46 | 4.82 | 3.90 | 5.03 | -1.24 | -1.44 | -4.74 | 2.56 | 3.66 | 6.56 |
Nuclear Periphery Foci | 2.08 | -1.25 | 2.35 | 1.18 | 5.38 | 3.60 | 4.32 | 3.95 | 1.96 | -1.74 | 3.75 | 2.30 | 4.08 | 2.17 | 4.52 |
Nucleolus | -0.04 | 0.41 | 1.20 | 1.90 | 10.84 | 0.03 | 3.83 | 3.83 | 2.87 | 0.02 | 0.23 | 3.23 | 3.98 | 3.38 | 10.55 |
Nucleus | 3.23 | -9.45 | 0.08 | -6.93 | 13.43 | -0.40 | -7.51 | -7.04 | -7.12 | -0.94 | 0.55 | -18.18 | -8.40 | -9.79 | 10.12 |
Peroxisomes | -0.36 | -0.68 | 1.65 | 2.17 | 2.79 | 2.67 | 1.52 | 0.59 | -3.97 | -1.67 | 2.46 | 1.58 | 1.66 | -2.51 | 0.31 |
Vacuole | 2.79 | 4.36 | 10.38 | 18.56 | 25.87 | 0.44 | 42.93* | 35.28* | 36.05* | -7.15 | 3.06 | 31.09 | 38.79 | 41.08* | 13.81 |
Vacuole Periphery | -2.00 | 1.86 | 2.32 | 9.01 | 5.67 | 8.50 | 19.66 | 19.45 | 16.21 | -1.84 | 9.03 | 19.30 | 19.53 | 18.60 | 3.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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