ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.15 0.21 0.15 0 0.05 – 0 0 0 0 0 0.22 0.16 0.21 – 0.11 – 0.09 0.13 0.15 0.11 0.23 0.23
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0.1 – 0 – 0 0 0 0 0.06 0.14
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0.08 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0.05 0.05 0.05 0 0 – 0 0 0 0 0 0.1 0.11 0.12 – 0 – 0 0 0 0 0 0
Cytoplasm 0.06 0.12 0.05 0.12 0.11 0.15 – 0.14 0.25 0.26 0.32 0.3 0.16 0.41 0.54 – 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.22 0.31 0.23 0.22 0.23 0.17 – 0.05 0.06 0 0.05 0 0.33 0.19 0.08 – 0.05 – 0.11 0.07 0.12 0.15 0.14 0.16
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0.06 0 0 0 0
Golgi 0.05 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Mitochondria 0 0.12 0 0 0 0 – 0 0 0 0.05 0 0 0 0 – 0 – 0 0 0 0.09 0.1 0.05
Nucleus 0.13 0.06 0.06 0.09 0 0.11 – 0.21 0.18 0.17 0.12 0.12 0.05 0.06 0 – 0.16 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.25 0.5 0.47 0.63 0.61 – 0.71 0.66 0.65 0.64 0.65 0.26 0.16 0.1 – 0.74 – 0.65 0.5 0.62 0.47 0.33 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 10 47 33 11 17 – 3 7 8 2 8 77 50 73 – 2 – 14 15 42 7 32 40
Bud 1 2 5 9 0 0 – 3 2 0 2 7 26 20 34 – 0 – 5 4 2 2 8 24
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 1 3 – 0 – 5 8 4 1 4 6
Bud Site 2 0 1 0 1 0 – 1 0 0 0 0 0 2 3 – 0 – – – – – – –
Cell Periphery 6 3 12 11 6 8 – 1 8 7 4 3 33 33 41 – 0 – 1 0 5 2 4 1
Cytoplasm 8 8 12 26 35 56 – 99 152 151 161 161 54 126 187 – 0 – 0 3 1 1 1 2
Endoplasmic Reticulum 30 20 52 46 76 64 – 38 37 25 27 23 113 58 29 – 1 – 18 7 32 10 19 27
Endosome 0 1 2 3 0 0 – 1 3 2 0 4 7 2 1 – 0 – 0 7 1 0 2 5
Golgi 7 0 6 8 21 4 – 6 2 0 1 5 3 0 0 – 1 – 5 1 5 1 2 4
Mitochondria 6 8 9 4 10 4 – 17 19 19 26 10 2 0 0 – 0 – 4 4 7 6 13 8
Nucleus 18 4 14 19 9 39 – 145 110 97 62 66 17 17 8 – 3 – 0 0 0 0 0 0
Nuclear Periphery 2 1 1 0 2 2 – 2 7 1 0 2 0 1 0 – 0 – 0 1 0 0 1 1
Nucleolus 1 1 0 0 0 3 – 3 4 4 4 5 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 1 0 5 2 0 1 – 0 0 0 0 2 0 0 1 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 2 2 0 0 – 0 0 0 0 0 2 6 12 – 1 – 0 0 0 0 0 0
Vac/Vac Membrane 77 16 114 101 206 225 – 501 395 378 321 348 90 50 33 – 14 – 103 57 171 30 46 53
Unique Cell Count 138 65 228 213 329 370 704 601 581 501 532 345 308 344 19 160 115 278 66 139 179
Labelled Cell Count 177 74 282 264 377 423 820 746 692 610 644 424 366 425 22 160 115 278 66 139 179


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 7.6 11.2 12.4 16.5 15.8 – 15.5 15.6 14.7 14.3 14.6 8.7 7.4 6.8 – 10.8 – 12.3 12.3 12.2
Std Deviation (1e-4) 3.2 2.2 3.1 2.9 3.6 3.0 – 3.5 3.5 3.5 3.5 3.6 1.6 1.5 1.3 – 1.8 – 3.1 2.8 3.0
Intensity Change (Log2) – – – 0.15 0.56 0.5 – 0.47 0.47 0.39 0.35 0.38 -0.36 -0.6 -0.71 – -0.05 – 0.14 0.13 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -6.6 -6.2 – -11.8 -10.1 -9.8 -10.1 -9.3 0.5 -1.3 0.2 – 0 –
Bud 1.2 0 0 – 0 0 0 0 0 2.8 2.3 3.6 – 0 –
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery 0 -2.3 -2.0 – 0 -3.3 -3.4 -3.8 0 1.9 2.2 2.7 – 0 –
Cytoplasm 2.6 2.2 3.7 – 3.6 6.5 6.6 7.9 7.5 3.8 9.3 12.1 – 0 –
Endoplasmic Reticulum -0.3 0.1 -1.7 – -7.7 -6.9 -8.1 -7.0 -7.8 2.6 -1.1 -4.8 – 0 –
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Golgi 0.7 2.0 0 – 0 0 0 0 0 0 0 0 – 0 –
Mitochondria -1.3 -0.6 0 – -1.2 -0.6 -0.5 0.7 -1.7 0 0 0 – 0 –
Nucleus 1.1 -2.0 1.8 – 5.0 4.4 3.9 2.6 2.6 -0.6 -0.3 -2.3 – 0 –
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – 0 –
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 2.0 – 0 –
Vacuole -0.5 3.0 2.6 – 5.9 4.2 3.9 3.6 4.0 -5.9 -8.4 -10.8 – 2.0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5426 3.1057 3.1627 2.5457 1.7561 3.2813 3.4632 3.9292 3.3494 3.2312 2.4931 3.521 2.8986 3.4159 3.0204 2.8864 2.9919 3.4075
Actin 0.0297 0.005 0.0196 0.0073 0.0009 0.0094 0.0637 0.0321 0.0471 0.0672 0.0011 0.018 0.0761 0.0109 0.0585 0.0277 0.0116 0.0141
Bud 0.0318 0.0176 0.0722 0.018 0.0259 0.0039 0.0107 0.0081 0.0345 0.0369 0.0052 0.0078 0.0157 0.0202 0.0579 0.0191 0.0333 0.0059
Bud Neck 0.0274 0.0119 0.013 0.0055 0.0245 0.2664 0.0546 0.019 0.0142 0.0272 0.06 0.2893 0.0385 0.0299 0.0295 0.0049 0.0209 0.3655
Bud Periphery 0.1144 0.0463 0.2673 0.1201 0.0952 0.0197 0.0384 0.0216 0.1726 0.3324 0.0285 0.0332 0.0694 0.0599 0.2005 0.1702 0.1551 0.0238
Bud Site 0.0566 0.1626 0.0374 0.0198 0.0199 0.0059 0.0892 0.2585 0.0636 0.0104 0.0068 0.0092 0.0611 0.1446 0.0536 0.0059 0.0164 0.0056
Cell Periphery 0.0418 0.03 0.0218 0.0204 0.0076 0.0221 0.0641 0.0347 0.0236 0.0348 0.0222 0.0273 0.0478 0.0234 0.021 0.0221 0.0617 0.0279
Cytoplasm 0.1595 0.0796 0.0746 0.1351 0.1937 0.1213 0.0862 0.1143 0.0766 0.014 0.1305 0.1066 0.1166 0.1092 0.087 0.1102 0.0789 0.0855
Cytoplasmic Foci 0.0405 0.0283 0.0135 0.0093 0.0214 0.0225 0.0377 0.039 0.0299 0.004 0.0109 0.021 0.0461 0.0343 0.02 0.0146 0.0115 0.0206
Eisosomes 0.0004 0.0002 0.0001 0.0001 0 0.0003 0.0045 0.0004 0.0006 0.0001 0.0001 0.0002 0.0013 0.0002 0.0004 0.0002 0.0007 0.0001
Endoplasmic Reticulum 0.0132 0.0144 0.0142 0.0214 0.0148 0.0109 0.0248 0.0091 0.0173 0.0204 0.013 0.0152 0.0176 0.0213 0.0109 0.0169 0.0382 0.0246
Endosome 0.1609 0.135 0.0779 0.1766 0.1809 0.104 0.0934 0.0838 0.119 0.0825 0.1005 0.1073 0.1473 0.1374 0.1071 0.1378 0.1486 0.073
Golgi 0.019 0.0177 0.0055 0.0122 0.0109 0.012 0.0311 0.0105 0.0229 0.0072 0.0055 0.0157 0.0204 0.0126 0.016 0.0078 0.0176 0.0096
Lipid Particles 0.0167 0.0159 0.0035 0.0057 0.0034 0.005 0.0484 0.0081 0.018 0.0032 0.0125 0.0082 0.0324 0.0229 0.0053 0.0041 0.0177 0.008
Mitochondria 0.0054 0.0061 0.0119 0.0084 0.006 0.0081 0.0118 0.0061 0.031 0.0199 0.0462 0.0065 0.0054 0.0064 0.0141 0.005 0.0092 0.0028
None 0.0202 0.0517 0.0523 0.0018 0.0043 0.014 0.0456 0.0823 0.0734 0.0008 0.0277 0.0093 0.0244 0.0522 0.0647 0.0601 0.0015 0.0043
Nuclear Periphery 0.0029 0.0025 0.0028 0.0052 0.0044 0.0021 0.0228 0.0028 0.0052 0.0044 0.0037 0.002 0.0051 0.0038 0.0029 0.0031 0.0056 0.0032
Nucleolus 0.0016 0.0034 0.0023 0.0018 0.0022 0.0016 0.0048 0.002 0.0015 0.0052 0.0023 0.0013 0.0014 0.0022 0.0017 0.0014 0.0013 0.0012
Nucleus 0.0028 0.0072 0.004 0.0054 0.0128 0.0038 0.0107 0.0037 0.0033 0.0071 0.007 0.0033 0.003 0.0047 0.0031 0.0033 0.0034 0.0031
Peroxisomes 0.0014 0.0014 0.0009 0.0003 0.0012 0.0008 0.0087 0.0029 0.0027 0.0004 0.0027 0.0008 0.0028 0.002 0.0013 0.0004 0.0005 0.0006
Punctate Nuclear 0.0006 0.0009 0.0006 0.0003 0.0008 0.001 0.0074 0.0008 0.0011 0.0002 0.0008 0.0005 0.002 0.001 0.0007 0.0003 0.0003 0.0004
Vacuole 0.2385 0.3478 0.2717 0.3892 0.3537 0.3457 0.2257 0.2512 0.2163 0.2868 0.4788 0.3049 0.2499 0.2782 0.2178 0.3492 0.3454 0.3097
Vacuole Periphery 0.0148 0.0145 0.0329 0.0364 0.0155 0.0195 0.0158 0.0092 0.0256 0.035 0.0338 0.0127 0.0157 0.0229 0.026 0.0356 0.0206 0.0104

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.9752 21.443 17.2376 53.0974 51.4018 35.2947 22.7349 15.4461 36.7068 64.6492
Translational Efficiency 1.2398 1.4774 1.1829 0.8412 1.0008 1.434 0.8496 0.9949 1.0429 0.949

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; YOL019W has a paralog, DCV1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (55%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YOL019W

YOL019W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yol019w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available