Standard name | |
---|---|
Human Ortholog | |
Description | High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 18 | 16 | 31 | 29 | 5 | 5 | 1 | 7 | – | 1 | 5 | 4 | 32 | 30 | 12 | 1 | 1 | 2 | 3 | 5 | 4 | 7 | 12 | 7 |
Bud | 13 | 20 | 9 | 16 | 5 | 7 | 15 | 15 | – | 14 | 29 | 16 | 8 | 8 | 7 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 3 | 3 | 3 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 166 | 129 | 99 | 114 | 45 | 43 | 27 | 31 | – | 23 | 29 | 35 | 179 | 179 | 163 | 14 | 2 | 12 | 66 | 72 | 66 | 161 | 289 | 240 |
Cytoplasm | 117 | 102 | 63 | 119 | 140 | 207 | 229 | 294 | – | 273 | 281 | 372 | 43 | 43 | 42 | 90 | 33 | 70 | 49 | 32 | 8 | 11 | 9 | 7 |
Endoplasmic Reticulum | 28 | 13 | 27 | 14 | 60 | 25 | 13 | 10 | – | 9 | 1 | 7 | 7 | 6 | 8 | 3 | 0 | 0 | 141 | 96 | 88 | 78 | 67 | 80 |
Endosome | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 1 | 1 | 2 | 5 | 11 | 20 | – | 37 | 36 | 37 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 5 | 6 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 9 | 8 | 4 | 11 | – | 3 | 2 | 3 | 1 | 1 | 3 | 24 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | – | 2 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 25 | 38 | 73 | 71 | – | 37 | 29 | 38 | 0 | 0 | 0 | 9 | 4 | 7 | 24 | 8 | 23 | 12 | 10 | 13 |
Unique Cell Count | 257 | 202 | 179 | 205 | 245 | 285 | 316 | 390 | 329 | 333 | 444 | 209 | 207 | 205 | 124 | 41 | 91 | 293 | 226 | 200 | 285 | 408 | 363 | |
Labelled Cell Count | 343 | 280 | 233 | 294 | 296 | 339 | 377 | 462 | 399 | 417 | 522 | 270 | 267 | 235 | 145 | 45 | 101 | 293 | 226 | 200 | 285 | 408 | 363 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.6 | 16.2 | 13.4 | 12.9 | 10.9 | 8.5 | 7.1 | 6.8 | – | 5.3 | 5.4 | 5.3 | 14.4 | 14.4 | 13.7 | 9.2 | 9.0 | 12.6 | 17.3 | 16.8 | 17.0 |
Std Deviation (1e-4) | 2.6 | 2.9 | 2.3 | 2.4 | 2.2 | 2.5 | 2.4 | 2.3 | – | 2.1 | 2.0 | 1.9 | 2.5 | 2.5 | 2.2 | 1.6 | 2.2 | 2.7 | 3.1 | 2.7 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.3 | -0.65 | -0.92 | -0.98 | – | -1.34 | -1.31 | -1.33 | 0.11 | 0.11 | 0.03 | -0.54 | -0.58 | -0.09 | 0.37 | 0.33 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -5.6 | -6.1 | -7.4 | -6.9 | – | -7.5 | -6.7 | -8.1 | -0.5 | -0.8 | -3.6 | -4.6 | -2.4 | -3.6 |
Bud | 1.1 | -1.7 | -1.5 | -0.1 | -0.7 | – | -0.4 | 1.5 | -0.8 | -0.6 | -0.6 | -0.8 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -7.9 | -9.2 | -11.5 | -12.5 | – | -12.2 | -11.6 | -13.0 | 6.6 | 6.8 | 5.1 | -7.8 | -5.8 | -6.6 |
Cytoplasm | 4.5 | 4.5 | 8.0 | 8.1 | 9.2 | – | 10.9 | 11.3 | 12.0 | -3.2 | -3.2 | -3.2 | 6.4 | 5.3 | 6.5 |
Endoplasmic Reticulum | -2.6 | 2.4 | -2.1 | -4.3 | -5.6 | – | -5.2 | -7.0 | -6.7 | -4.1 | -4.3 | -3.8 | -3.6 | -2.7 | -3.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.7 | – | 4.4 | 4.3 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 6.1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.1 | 4.8 | 6.8 | 5.9 | – | 4.4 | 3.7 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.4822 | 21.5009 | 19.2671 | 16.8984 | 15.1623 | 19.1814 | 16.7519 | 24.7564 | 20.0802 | 19.9436 | 19.8145 | 20.5397 | 19.2909 | 26.541 | 22.4238 | 21.2746 | 20.6965 | 22.5118 |
Actin | 0.019 | 0.0004 | 0.0004 | 0.0118 | 0.0155 | 0.0003 | 0.027 | 0.0003 | 0.0142 | 0.0094 | 0.0065 | 0.0011 | 0.0197 | 0.0001 | 0.0051 | 0.0023 | 0.0151 | 0.0095 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0036 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0.0005 |
Bud Neck | 0.0013 | 0.0009 | 0.0025 | 0.0042 | 0.0022 | 0.0021 | 0.0012 | 0.0013 | 0.0021 | 0.0043 | 0.0015 | 0.0026 | 0.0008 | 0.0008 | 0.0031 | 0.0035 | 0.0108 | 0.0064 |
Bud Periphery | 0.0026 | 0.0008 | 0.0025 | 0.0254 | 0.002 | 0.003 | 0.0028 | 0.0013 | 0.0071 | 0.0039 | 0.0039 | 0.0029 | 0.0042 | 0.0014 | 0.0041 | 0.0082 | 0.0183 | 0.008 |
Bud Site | 0.0017 | 0.0027 | 0.0009 | 0.0045 | 0.0028 | 0.0003 | 0.0041 | 0.0024 | 0.0167 | 0.0006 | 0.001 | 0.0002 | 0.0015 | 0.0016 | 0.0022 | 0.0003 | 0.0018 | 0.001 |
Cell Periphery | 0.5332 | 0.5352 | 0.5126 | 0.4255 | 0.3909 | 0.5575 | 0.4624 | 0.6372 | 0.5298 | 0.5089 | 0.5162 | 0.5405 | 0.5721 | 0.6891 | 0.6613 | 0.6492 | 0.6503 | 0.6633 |
Cytoplasm | 0.0548 | 0.1047 | 0.0983 | 0.1483 | 0.0438 | 0.0786 | 0.0175 | 0.0438 | 0.018 | 0.0214 | 0.0315 | 0.0265 | 0.0389 | 0.0641 | 0.0264 | 0.0327 | 0.047 | 0.0236 |
Cytoplasmic Foci | 0.0054 | 0.0004 | 0.0004 | 0.0024 | 0.0066 | 0.0004 | 0.0066 | 0.0003 | 0.0064 | 0.0069 | 0.0064 | 0.002 | 0.0036 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0003 |
Eisosomes | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0008 | 0.0001 | 0.0111 | 0.0004 | 0.0054 | 0.016 | 0.0005 | 0.0003 | 0.0094 | 0.0051 | 0.017 | 0.0015 | 0.0034 | 0.0066 |
Endoplasmic Reticulum | 0.1395 | 0.1827 | 0.2063 | 0.102 | 0.2729 | 0.1796 | 0.2229 | 0.1898 | 0.2234 | 0.2337 | 0.205 | 0.2307 | 0.2285 | 0.1588 | 0.1937 | 0.2107 | 0.122 | 0.1752 |
Endosome | 0.0117 | 0.001 | 0.001 | 0.0258 | 0.0119 | 0.0015 | 0.0212 | 0.0001 | 0.0031 | 0.0065 | 0.035 | 0.0037 | 0.0021 | 0 | 0.0006 | 0.0003 | 0.0006 | 0.0003 |
Golgi | 0.0031 | 0.0006 | 0.0003 | 0.0026 | 0.0062 | 0.0006 | 0.0101 | 0.0003 | 0.0045 | 0.0049 | 0.0077 | 0.001 | 0.0025 | 0.0002 | 0.0016 | 0.0008 | 0.0022 | 0.0008 |
Lipid Particles | 0.0434 | 0.0667 | 0.0459 | 0.0405 | 0.07 | 0.035 | 0.0475 | 0.0305 | 0.0442 | 0.0444 | 0.0397 | 0.0513 | 0.0185 | 0.0149 | 0.0118 | 0.0086 | 0.0164 | 0.0208 |
Mitochondria | 0.0056 | 0.0002 | 0.0003 | 0.0042 | 0.005 | 0.0001 | 0.0183 | 0 | 0.008 | 0.001 | 0.0028 | 0.0003 | 0.0016 | 0 | 0.0007 | 0 | 0.0312 | 0.0061 |
None | 0.0085 | 0.0002 | 0.0028 | 0.0023 | 0.0024 | 0.0001 | 0.0056 | 0.0001 | 0.0015 | 0.0003 | 0.0075 | 0.0002 | 0.0013 | 0.0002 | 0.0034 | 0.0001 | 0.0004 | 0.0009 |
Nuclear Periphery | 0.0143 | 0.0062 | 0.0069 | 0.0259 | 0.0123 | 0.0054 | 0.025 | 0.0129 | 0.0116 | 0.0091 | 0.0193 | 0.0063 | 0.015 | 0.0107 | 0.0121 | 0.0083 | 0.0079 | 0.0067 |
Nucleolus | 0.0009 | 0.0002 | 0.0004 | 0.0011 | 0.0145 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0002 |
Nucleus | 0.0034 | 0.0003 | 0.0009 | 0.0074 | 0.0044 | 0.0003 | 0.0012 | 0.0003 | 0.0006 | 0.0003 | 0.0041 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0012 | 0.0004 |
Peroxisomes | 0.0034 | 0 | 0 | 0.0006 | 0.0269 | 0 | 0.0026 | 0 | 0.0162 | 0.0037 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0039 | 0 | 0.0001 | 0.0038 | 0.0012 | 0 | 0.0055 | 0 | 0.0007 | 0.0006 | 0.0067 | 0.0006 | 0.006 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Vacuole | 0.1423 | 0.0963 | 0.1163 | 0.1548 | 0.1021 | 0.1344 | 0.1048 | 0.0787 | 0.0838 | 0.1233 | 0.1016 | 0.1288 | 0.0723 | 0.0523 | 0.0551 | 0.0719 | 0.0658 | 0.0689 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0005 | 0.003 | 0.005 | 0.0003 | 0.0017 | 0.0002 | 0.0008 | 0.0004 | 0.0016 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0005 | 0.0026 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.8596 | 47.6832 | 53.8973 | 53.0204 | 54.2812 | 48.3518 | 57.7428 | 52.5939 | 60.8324 | 65.1256 |
Translational Efficiency | 0.7881 | 0.848 | 0.6843 | 0.7681 | 0.7191 | 0.823 | 0.6577 | 0.734 | 0.6564 | 0.5586 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
95 | 431 | 1752 | 1640 | 162 | 1960 | 2868 | 1748 | 257 | 2391 | 4620 | 3388 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1137.65 | 1523.77 | 1526.85 | 1296.16 | 1283.72 | 1462.20 | 1693.21 | 1506.04 | 1229.73 | 1473.30 | 1630.12 | 1404.45 |
Standard Deviation | 142.57 | 237.88 | 207.52 | 239.21 | 197.04 | 221.69 | 213.44 | 231.21 | 192.25 | 225.94 | 226.11 | 257.45 |
Intensity Change Log 2 | — | 0.421588 | 0.424502 | 0.188187 | — | 0.187810 | 0.399430 | 0.230430 | — | 0.302376 | 0.411264 | 0.210736 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005683 | 0.001943 | 0.010079 | 0.004880 | 0.000422 | 0.002157 | 0.001721 | 0.007032 | 0.002367 | 0.002118 | 0.004891 | 0.005990 |
Bud Neck | 0.011972 | 0.003709 | 0.011834 | 0.009088 | 0.003878 | 0.003515 | 0.001827 | 0.006336 | 0.006870 | 0.003550 | 0.005622 | 0.007668 |
Bud Site | 0.041257 | 0.013037 | 0.044119 | 0.043334 | 0.011334 | 0.013580 | 0.009357 | 0.031043 | 0.022395 | 0.013482 | 0.022540 | 0.036993 |
Cell Periphery | 0.067797 | 0.784045* | 0.548704* | 0.548151* | 0.500841* | 0.713050* | 0.785651* | 0.668108* | 0.340766* | 0.725847* | 0.695796* | 0.610042* |
Cytoplasm | 0.180669* | 0.020974 | 0.168736* | 0.194144* | 0.054604 | 0.056397 | 0.087521 | 0.163985* | 0.101203* | 0.050011 | 0.118319* | 0.178584* |
Cytoplasmic Foci | 0.037717 | 0.000616 | 0.003024 | 0.004377 | 0.006797 | 0.004144 | 0.000836 | 0.002755 | 0.018226 | 0.003508 | 0.001665 | 0.003540 |
Eisosomes | 0.006027 | 0.001649 | 0.002145 | 0.001653 | 0.000280 | 0.001896 | 0.000532 | 0.001869 | 0.002404 | 0.001851 | 0.001144 | 0.001764 |
Endoplasmic Reticulum | 0.032790 | 0.066098 | 0.051717 | 0.062312 | 0.075893 | 0.058969 | 0.007913 | 0.006221 | 0.059960 | 0.060254 | 0.024524 | 0.033373 |
Endosome | 0.028373 | 0.001872 | 0.002122 | 0.002693 | 0.003551 | 0.002951 | 0.000707 | 0.002883 | 0.012726 | 0.002757 | 0.001244 | 0.002791 |
Golgi | 0.005600 | 0.001407 | 0.001919 | 0.002168 | 0.001390 | 0.002259 | 0.000562 | 0.004172 | 0.002947 | 0.002105 | 0.001077 | 0.003202 |
Lipid Particles | 0.017313 | 0.005645 | 0.039404 | 0.017356 | 0.001746 | 0.009242 | 0.006857 | 0.014171 | 0.007500 | 0.008594 | 0.019200 | 0.015713 |
Mitochondria | 0.001883 | 0.000253 | 0.000289 | 0.000736 | 0.000062 | 0.000899 | 0.000328 | 0.001115 | 0.000735 | 0.000783 | 0.000313 | 0.000932 |
Mitotic Spindle | 0.000520 | 0.001381 | 0.000698 | 0.000738 | 0.000061 | 0.001439 | 0.000849 | 0.001408 | 0.000231 | 0.001428 | 0.000792 | 0.001084 |
None | 0.000019 | 0.000013 | 0.000123 | 0.000067 | 0.000001 | 0.000161 | 0.000027 | 0.000056 | 0.000008 | 0.000134 | 0.000063 | 0.000061 |
Nuclear Periphery | 0.000263 | 0.006165 | 0.003380 | 0.003207 | 0.000344 | 0.009600 | 0.007319 | 0.001408 | 0.000314 | 0.008981 | 0.005825 | 0.002279 |
Nuclear Periphery Foci | 0.003306 | 0.001512 | 0.002578 | 0.002019 | 0.001232 | 0.001480 | 0.000667 | 0.001060 | 0.001999 | 0.001486 | 0.001391 | 0.001524 |
Nucleolus | 0.000084 | 0.000034 | 0.000051 | 0.000069 | 0.000027 | 0.000136 | 0.000088 | 0.000067 | 0.000048 | 0.000117 | 0.000074 | 0.000068 |
Nucleus | 0.007995 | 0.000334 | 0.002244 | 0.001637 | 0.000171 | 0.000730 | 0.000744 | 0.001507 | 0.003063 | 0.000658 | 0.001312 | 0.001570 |
Peroxisomes | 0.001993 | 0.004740 | 0.003446 | 0.005649 | 0.000945 | 0.018316 | 0.003549 | 0.001776 | 0.001332 | 0.015869 | 0.003510 | 0.003651 |
Vacuole | 0.541590* | 0.083559 | 0.102551 | 0.094616 | 0.334812* | 0.097984 | 0.081143 | 0.080601 | 0.411248* | 0.095384 | 0.089261 | 0.087385 |
Vacuole Periphery | 0.007149 | 0.001017 | 0.000837 | 0.001104 | 0.001612 | 0.001096 | 0.001801 | 0.002426 | 0.003659 | 0.001081 | 0.001436 | 0.001786 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.07 | -2.89 | 0.28 | -3.09 | 5.12 | -4.83 | -4.80 | -6.59 | -4.68 | -5.16 | 0.20 | -4.07 | -4.57 | -5.79 | -1.54 |
Bud Neck | 3.49 | -0.34 | 0.95 | -5.76 | 2.62 | 0.40 | 2.35 | -2.49 | -4.50 | -7.79 | 3.05 | 0.92 | -1.11 | -7.94 | -3.76 |
Bud Site | 2.25 | -0.45 | -0.35 | -6.39 | 0.31 | -0.39 | 0.33 | -3.34 | -6.03 | -8.26 | 1.46 | -0.20 | -2.70 | -9.87 | -6.44 |
Cell Periphery | -42.75* | -30.66* | -30.64* | 18.89 | -0.07 | -9.91 | -13.41 | -7.44 | 5.03 | 13.38 | -19.50 | -18.02 | -13.44 | 15.92 | 12.14 |
Cytoplasm | 7.76 | 0.17 | -1.06 | -25.61 | -2.98 | -0.17 | -3.13 | -9.62 | -17.21 | -11.45 | 4.66 | -1.91 | -7.37 | -27.25 | -11.54 |
Cytoplasmic Foci | 6.92 | 6.45 | 6.20 | -7.63 | -2.18 | 0.58 | 1.33 | 0.88 | 1.52 | -4.09 | 4.10 | 4.66 | 4.12 | -0.11 | -5.11 |
Eisosomes | 1.46 | 1.28 | 1.46 | -0.02 | 2.70 | -8.72 | -1.76 | -7.37 | 0.11 | -8.02 | 0.37 | 1.08 | 0.45 | 0.55 | -5.20 |
Endoplasmic Reticulum | -2.65 | -1.74 | -2.55 | 0.51 | -2.13 | 1.58 | 6.55 | 6.71 | 19.03 | 1.71 | -0.15 | 4.25 | 3.10 | 8.43 | -3.74 |
Endosome | 4.41 | 4.37 | 4.26 | -2.27 | -1.60 | 0.37 | 1.80 | 0.39 | 0.09 | -3.89 | 3.87 | 4.52 | 3.84 | -0.12 | -4.62 |
Golgi | 0.99 | 0.85 | 0.78 | -1.48 | -0.58 | -0.90 | 0.93 | -2.08 | -1.76 | -3.58 | 0.43 | 1.13 | -0.27 | -1.68 | -3.78 |
Lipid Particles | 2.37 | -4.86 | -0.30 | -6.77 | 8.31 | -7.45 | -6.23 | -8.04 | -2.91 | -4.56 | -0.87 | -6.34 | -4.58 | -5.83 | 2.48 |
Mitochondria | 1.05 | 1.02 | 0.69 | -1.65 | -1.53 | -6.99 | -8.04 | -3.72 | -0.70 | -2.77 | -0.21 | 0.70 | -0.47 | -0.66 | -3.04 |
Mitotic Spindle | -2.81 | -0.71 | -0.84 | 5.16 | -0.68 | -20.32* | -24.61* | -2.54 | 0.01 | -1.08 | -9.62 | -5.08 | -2.92 | 1.16 | -1.08 |
None | 0.71 | -2.00 | -4.17 | -8.40 | 1.10 | -1.21 | -8.36 | -4.53 | 0.80 | -2.38 | -1.17 | -2.83 | -6.81 | 0.69 | 0.06 |
Nuclear Periphery | -10.17 | -16.52 | -16.63 | 4.96 | 0.71 | -25.77* | -22.91 | -11.53 | 22.44* | 18.98 | -27.63 | -27.01 | -20.15 | 20.73 | 16.23 |
Nuclear Periphery Foci | 3.58 | 1.28 | 2.43 | -2.90 | 3.14 | -1.55 | 3.68 | 0.82 | 3.63 | -4.35 | 2.26 | 2.68 | 1.90 | -0.52 | -1.43 |
Nucleolus | 1.73 | 1.09 | 0.46 | -8.57 | -5.07 | -1.45 | -11.16 | -1.54 | 0.61 | 0.03 | -1.15 | -2.55 | -1.38 | 0.64 | -0.19 |
Nucleus | 2.04 | 1.53 | 1.69 | -17.44 | 4.99 | -4.98 | -10.95 | -9.43 | -4.86 | -5.52 | 1.71 | 1.24 | 1.02 | -7.89 | -3.10 |
Peroxisomes | -3.51 | -3.21 | -6.39 | -1.14 | -4.72 | -15.49 | -6.73 | -2.88 | 15.05 | 5.63 | -15.54 | -7.36 | -7.53 | 13.09 | -0.50 |
Vacuole | 15.00 | 14.58 | 14.83 | -1.70 | 1.96 | 12.55 | 13.52 | 13.42 | 3.89 | -0.08 | 17.92 | 18.36 | 18.40 | 2.20 | 0.55 |
Vacuole Periphery | 0.95 | 0.99 | 0.94 | -0.63 | -2.35 | 1.45 | -0.54 | -1.19 | -2.26 | -1.06 | 1.09 | 0.93 | 0.76 | -2.27 | -1.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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