Standard name
Human Ortholog
Description High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.08 0.17 0.14 0 0 0 0 – 0 0 0 0.15 0.14 0.06 0 0 0 0 0 0 0 0 0
Bud 0.05 0.1 0.05 0.08 0 0 0.05 0 – 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.65 0.64 0.55 0.56 0.18 0.15 0.09 0.08 – 0.07 0.09 0.08 0.86 0.86 0.8 0.11 0.05 0.13 0.23 0.32 0.33 0.57 0.71 0.66
Cytoplasm 0.46 0.5 0.35 0.58 0.57 0.73 0.72 0.75 – 0.83 0.84 0.84 0.21 0.21 0.2 0.73 0.8 0.77 0.17 0.15 0 0 0 0
Endoplasmic Reticulum 0.11 0.06 0.15 0.07 0.24 0.09 0 0 – 0 0 0 0 0 0 0 0 0 0.48 0.43 0.44 0.27 0.16 0.22
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.05 – 0.11 0.11 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.19 0.07 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.13 0.23 0.18 – 0.11 0.09 0.09 0 0 0 0.07 0.1 0.08 0.08 0 0.12 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 16 31 29 5 5 1 7 – 1 5 4 32 30 12 1 1 2 3 5 4 7 12 7
Bud 13 20 9 16 5 7 15 15 – 14 29 16 8 8 7 1 0 1 0 0 0 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 6 6 3 3 3 1
Bud Site 0 0 0 0 0 1 0 0 – 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 166 129 99 114 45 43 27 31 – 23 29 35 179 179 163 14 2 12 66 72 66 161 289 240
Cytoplasm 117 102 63 119 140 207 229 294 – 273 281 372 43 43 42 90 33 70 49 32 8 11 9 7
Endoplasmic Reticulum 28 13 27 14 60 25 13 10 – 9 1 7 7 6 8 3 0 0 141 96 88 78 67 80
Endosome 0 0 0 0 5 0 1 0 – 0 0 0 0 0 0 1 0 1 0 1 0 0 1 0
Golgi 1 0 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 1 1 2 5 11 20 – 37 36 37 0 0 0 1 2 1 0 0 3 5 6 3
Nucleus 0 0 0 0 9 8 4 11 – 3 2 3 1 1 3 24 3 7 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 3 3 – 2 1 6 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 2 2 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 25 38 73 71 – 37 29 38 0 0 0 9 4 7 24 8 23 12 10 13
Unique Cell Count 257 202 179 205 245 285 316 390 329 333 444 209 207 205 124 41 91 293 226 200 285 408 363
Labelled Cell Count 343 280 233 294 296 339 377 462 399 417 522 270 267 235 145 45 101 293 226 200 285 408 363


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 16.2 13.4 12.9 10.9 8.5 7.1 6.8 – 5.3 5.4 5.3 14.4 14.4 13.7 9.2 9.0 12.6 17.3 16.8 17.0
Std Deviation (1e-4) 2.6 2.9 2.3 2.4 2.2 2.5 2.4 2.3 – 2.1 2.0 1.9 2.5 2.5 2.2 1.6 2.2 2.7 3.1 2.7 2.9
Intensity Change (Log2) – – – -0.06 -0.3 -0.65 -0.92 -0.98 – -1.34 -1.31 -1.33 0.11 0.11 0.03 -0.54 -0.58 -0.09 0.37 0.33 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -5.6 -6.1 -7.4 -6.9 – -7.5 -6.7 -8.1 -0.5 -0.8 -3.6 -4.6 -2.4 -3.6
Bud 1.1 -1.7 -1.5 -0.1 -0.7 – -0.4 1.5 -0.8 -0.6 -0.6 -0.8 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -7.9 -9.2 -11.5 -12.5 – -12.2 -11.6 -13.0 6.6 6.8 5.1 -7.8 -5.8 -6.6
Cytoplasm 4.5 4.5 8.0 8.1 9.2 – 10.9 11.3 12.0 -3.2 -3.2 -3.2 6.4 5.3 6.5
Endoplasmic Reticulum -2.6 2.4 -2.1 -4.3 -5.6 – -5.2 -7.0 -6.7 -4.1 -4.3 -3.8 -3.6 -2.7 -3.9
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.7 – 4.4 4.3 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 6.1 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 4.1 4.8 6.8 5.9 – 4.4 3.7 3.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.4822 21.5009 19.2671 16.8984 15.1623 19.1814 16.7519 24.7564 20.0802 19.9436 19.8145 20.5397 19.2909 26.541 22.4238 21.2746 20.6965 22.5118
Actin 0.019 0.0004 0.0004 0.0118 0.0155 0.0003 0.027 0.0003 0.0142 0.0094 0.0065 0.0011 0.0197 0.0001 0.0051 0.0023 0.0151 0.0095
Bud 0.0004 0.0001 0.0002 0.0036 0.0004 0.0001 0.0004 0.0001 0.0013 0.0003 0.0003 0.0001 0.0005 0.0001 0.0003 0.0003 0.0011 0.0005
Bud Neck 0.0013 0.0009 0.0025 0.0042 0.0022 0.0021 0.0012 0.0013 0.0021 0.0043 0.0015 0.0026 0.0008 0.0008 0.0031 0.0035 0.0108 0.0064
Bud Periphery 0.0026 0.0008 0.0025 0.0254 0.002 0.003 0.0028 0.0013 0.0071 0.0039 0.0039 0.0029 0.0042 0.0014 0.0041 0.0082 0.0183 0.008
Bud Site 0.0017 0.0027 0.0009 0.0045 0.0028 0.0003 0.0041 0.0024 0.0167 0.0006 0.001 0.0002 0.0015 0.0016 0.0022 0.0003 0.0018 0.001
Cell Periphery 0.5332 0.5352 0.5126 0.4255 0.3909 0.5575 0.4624 0.6372 0.5298 0.5089 0.5162 0.5405 0.5721 0.6891 0.6613 0.6492 0.6503 0.6633
Cytoplasm 0.0548 0.1047 0.0983 0.1483 0.0438 0.0786 0.0175 0.0438 0.018 0.0214 0.0315 0.0265 0.0389 0.0641 0.0264 0.0327 0.047 0.0236
Cytoplasmic Foci 0.0054 0.0004 0.0004 0.0024 0.0066 0.0004 0.0066 0.0003 0.0064 0.0069 0.0064 0.002 0.0036 0.0001 0.0004 0.0003 0.0007 0.0003
Eisosomes 0.0005 0.0002 0.0003 0.0005 0.0008 0.0001 0.0111 0.0004 0.0054 0.016 0.0005 0.0003 0.0094 0.0051 0.017 0.0015 0.0034 0.0066
Endoplasmic Reticulum 0.1395 0.1827 0.2063 0.102 0.2729 0.1796 0.2229 0.1898 0.2234 0.2337 0.205 0.2307 0.2285 0.1588 0.1937 0.2107 0.122 0.1752
Endosome 0.0117 0.001 0.001 0.0258 0.0119 0.0015 0.0212 0.0001 0.0031 0.0065 0.035 0.0037 0.0021 0 0.0006 0.0003 0.0006 0.0003
Golgi 0.0031 0.0006 0.0003 0.0026 0.0062 0.0006 0.0101 0.0003 0.0045 0.0049 0.0077 0.001 0.0025 0.0002 0.0016 0.0008 0.0022 0.0008
Lipid Particles 0.0434 0.0667 0.0459 0.0405 0.07 0.035 0.0475 0.0305 0.0442 0.0444 0.0397 0.0513 0.0185 0.0149 0.0118 0.0086 0.0164 0.0208
Mitochondria 0.0056 0.0002 0.0003 0.0042 0.005 0.0001 0.0183 0 0.008 0.001 0.0028 0.0003 0.0016 0 0.0007 0 0.0312 0.0061
None 0.0085 0.0002 0.0028 0.0023 0.0024 0.0001 0.0056 0.0001 0.0015 0.0003 0.0075 0.0002 0.0013 0.0002 0.0034 0.0001 0.0004 0.0009
Nuclear Periphery 0.0143 0.0062 0.0069 0.0259 0.0123 0.0054 0.025 0.0129 0.0116 0.0091 0.0193 0.0063 0.015 0.0107 0.0121 0.0083 0.0079 0.0067
Nucleolus 0.0009 0.0002 0.0004 0.0011 0.0145 0.0001 0.0004 0.0001 0.0005 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.001 0.0002
Nucleus 0.0034 0.0003 0.0009 0.0074 0.0044 0.0003 0.0012 0.0003 0.0006 0.0003 0.0041 0.0003 0.0005 0.0002 0.0004 0.0003 0.0012 0.0004
Peroxisomes 0.0034 0 0 0.0006 0.0269 0 0.0026 0 0.0162 0.0037 0.0005 0.0001 0.0004 0 0.0001 0 0.0001 0.0001
Punctate Nuclear 0.0039 0 0.0001 0.0038 0.0012 0 0.0055 0 0.0007 0.0006 0.0067 0.0006 0.006 0 0.0001 0.0001 0.0002 0.0001
Vacuole 0.1423 0.0963 0.1163 0.1548 0.1021 0.1344 0.1048 0.0787 0.0838 0.1233 0.1016 0.1288 0.0723 0.0523 0.0551 0.0719 0.0658 0.0689
Vacuole Periphery 0.0011 0.0002 0.0005 0.003 0.005 0.0003 0.0017 0.0002 0.0008 0.0004 0.0016 0.0004 0.0005 0.0002 0.0004 0.0005 0.0026 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.8596 47.6832 53.8973 53.0204 54.2812 48.3518 57.7428 52.5939 60.8324 65.1256
Translational Efficiency 0.7881 0.848 0.6843 0.7681 0.7191 0.823 0.6577 0.734 0.6564 0.5586

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance
Localization
Cell Percentages cell periphery (26%), ER (17%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tat2

Tat2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tat2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available