Standard name | |
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Human Ortholog | |
Description | Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud | 1 | 0 | 3 | 0 | 2 | 2 | 6 | 8 | 9 | 11 | 8 | 8 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 7 | 6 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 2 | 1 | 16 | 10 | 15 | 5 | 12 | 5 | 12 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 10 | 14 | 8 | 9 | 17 | 24 | 29 | 16 | 23 | 13 | 9 | 36 | 63 | 71 | 21 | 11 | 12 | 0 | 1 | 0 | 2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 6 | 1 | 0 | 1 | 1 | 0 | 2 | 0 |
Endosome | 2 | 8 | 10 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 12 | 2 | 0 | 1 | 5 | 11 | 33 | 4 | 14 | 25 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 3 | 0 | 2 | 0 | 0 | 3 | 2 |
Mitochondria | 1 | 5 | 50 | 92 | 68 | 161 | 221 | 196 | 197 | 247 | 200 | 214 | 2 | 5 | 17 | 8 | 0 | 5 | 0 | 0 | 2 | 2 | 3 | 5 |
Nucleus | 65 | 103 | 240 | 252 | 335 | 326 | 402 | 499 | 272 | 318 | 276 | 271 | 146 | 189 | 209 | 214 | 99 | 131 | 49 | 108 | 155 | 69 | 80 | 68 |
Nuclear Periphery | 0 | 1 | 1 | 0 | 4 | 2 | 8 | 2 | 3 | 0 | 9 | 7 | 0 | 2 | 1 | 3 | 0 | 1 | 1 | 0 | 6 | 0 | 2 | 6 |
Nucleolus | 46 | 106 | 114 | 37 | 10 | 23 | 47 | 94 | 24 | 47 | 39 | 59 | 47 | 66 | 66 | 36 | 18 | 29 | 87 | 156 | 202 | 61 | 111 | 114 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 7 | 2 | 0 | 1 | 3 | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 5 | 14 | 37 | 2 | 13 | 15 |
Vac/Vac Membrane | 17 | 43 | 25 | 13 | 49 | 48 | 43 | 45 | 13 | 25 | 13 | 15 | 17 | 27 | 27 | 39 | 21 | 28 | 5 | 17 | 30 | 34 | 92 | 95 |
Unique Cell Count | 122 | 246 | 403 | 327 | 429 | 464 | 614 | 735 | 392 | 513 | 427 | 417 | 233 | 335 | 370 | 306 | 146 | 194 | 159 | 326 | 483 | 184 | 331 | 345 |
Labelled Cell Count | 135 | 278 | 464 | 407 | 478 | 597 | 767 | 890 | 539 | 685 | 563 | 597 | 249 | 359 | 411 | 333 | 160 | 211 | 159 | 326 | 483 | 184 | 331 | 345 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 10.8 | 6.8 | 6.6 | 6.3 | 5.9 | 5.6 | 6.0 | 5.5 | 5.4 | 5.3 | 5.2 | 11.2 | 10.6 | 9.4 | 14.9 | 15.8 | 16.0 | 11.7 | 12.5 | 12.8 |
Std Deviation (1e-4) | 2.4 | 2.1 | 1.2 | 1.2 | 1.0 | 1.0 | 1.0 | 1.1 | 1.3 | 1.1 | 1.1 | 1.3 | 2.6 | 2.7 | 2.3 | 3.0 | 3.6 | 3.6 | 2.1 | 2.3 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.11 | -0.22 | -0.29 | -0.2 | -0.3 | -0.34 | -0.38 | -0.38 | 0.71 | 0.63 | 0.45 | 1.13 | 1.2 | 1.23 | 0.77 | 0.87 | 0.9 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1.8 | 1.7 | 1.4 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.8 | 0 | 2.9 | 0 | 3.1 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.8 | -1.2 | 0.2 | 0.4 | 0.4 | 0.4 | 0.8 | -0.3 | -1.1 | 5.4 | 6.8 | 7.0 | 2.1 | 2.0 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | -3.0 | -2.8 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | 0.6 | -1.9 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.3 | 1.4 | 7.6 | 8.3 | 5.6 | 11.5 | 11.5 | 10.8 | 11.9 | -5.1 | -5.6 | -3.9 | -4.7 | -4.5 | -3.9 |
Nucleus | 5.0 | 5.8 | 3.3 | 1.9 | 2.8 | 2.9 | 0.7 | 1.5 | 1.6 | 0.8 | -0.9 | -0.9 | 2.9 | 1.8 | 1.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -5.6 | -10.5 | -9.4 | -8.8 | -6.5 | -8.2 | -7.5 | -7.1 | -5.0 | -2.3 | -2.7 | -3.4 | -5.3 | -3.9 | -3.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.3 | 2.6 | 2.2 | 0.5 | -0.1 | -1.9 | -0.9 | -2.2 | -1.7 | 0.5 | 1.0 | 0.6 | 3.0 | 3.1 | 3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.944 | 10.8986 | 9.4992 | 9.8479 | 8.4408 | 10.0471 | 10.1277 | 14.2913 | 11.6616 | 10.8933 | 10.3081 | 12.271 | 13.0156 | 13.0718 | 12.5164 | 11.941 | 12.3365 | 12.3436 |
Actin | 0.0202 | 0 | 0.0073 | 0.033 | 0.0047 | 0.0027 | 0.0068 | 0 | 0.0041 | 0.0221 | 0.0032 | 0.0003 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Bud | 0.0006 | 0 | 0.0003 | 0.001 | 0.001 | 0.0002 | 0.0006 | 0 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0032 | 0.0007 | 0.0013 | 0.0012 | 0.0131 | 0.0017 | 0.0013 | 0.0003 | 0.0011 | 0.0005 | 0.0006 | 0.0012 | 0.0004 | 0.0006 | 0.0006 | 0.0004 | 0.001 | 0.0019 |
Bud Periphery | 0.0011 | 0.0001 | 0.0005 | 0.0014 | 0.0017 | 0.0004 | 0.0008 | 0.0001 | 0.0005 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0003 |
Bud Site | 0.0027 | 0.0002 | 0.0051 | 0.001 | 0.004 | 0.0004 | 0.0119 | 0.0001 | 0.0023 | 0.0015 | 0.0007 | 0.0003 | 0.0003 | 0.0011 | 0.0007 | 0.0002 | 0.0006 | 0.0002 |
Cell Periphery | 0.0004 | 0.0002 | 0.0009 | 0.0007 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Cytoplasm | 0.002 | 0.0001 | 0.0007 | 0.0001 | 0.0021 | 0.0004 | 0.0054 | 0.0001 | 0.0015 | 0.002 | 0.0049 | 0.0005 | 0 | 0.0005 | 0.0028 | 0.0001 | 0.0002 | 0 |
Cytoplasmic Foci | 0.0095 | 0 | 0.0067 | 0.0002 | 0.0063 | 0.0008 | 0.0099 | 0 | 0.0021 | 0.008 | 0.0021 | 0.0004 | 0 | 0 | 0.0019 | 0 | 0.0002 | 0 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0025 | 0 | 0.001 | 0.0002 | 0.0026 | 0.0007 | 0.0022 | 0 | 0.0009 | 0.0029 | 0.0046 | 0.0003 | 0 | 0 | 0.0031 | 0 | 0.0002 | 0 |
Endosome | 0.0183 | 0.0001 | 0.005 | 0.0006 | 0.0063 | 0.0011 | 0.0099 | 0 | 0.0039 | 0.0176 | 0.0104 | 0.0019 | 0.0001 | 0.0001 | 0.0037 | 0 | 0.0015 | 0.0004 |
Golgi | 0.0043 | 0 | 0.006 | 0.0007 | 0.0016 | 0.0006 | 0.0042 | 0 | 0.0023 | 0.0068 | 0.0009 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0 |
Lipid Particles | 0.0108 | 0.0001 | 0.0129 | 0.0009 | 0.0044 | 0.0019 | 0.0139 | 0.0001 | 0.0022 | 0.0244 | 0.0043 | 0.0005 | 0.0001 | 0 | 0.0037 | 0 | 0.001 | 0.0003 |
Mitochondria | 0.0101 | 0.0127 | 0.0062 | 0.0048 | 0.0052 | 0.0006 | 0.0034 | 0.0002 | 0.0029 | 0.0045 | 0.016 | 0.0005 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0.0002 |
None | 0.0042 | 0 | 0.0004 | 0.0002 | 0.0034 | 0.0004 | 0.0152 | 0 | 0.0017 | 0.0026 | 0.0024 | 0.0006 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0213 | 0.0014 | 0.0043 | 0.0177 | 0.0121 | 0.0028 | 0.0149 | 0.0006 | 0.0081 | 0.016 | 0.034 | 0.0056 | 0.0002 | 0.0007 | 0.0034 | 0.0005 | 0.0041 | 0.0015 |
Nucleolus | 0.3651 | 0.4435 | 0.4681 | 0.3741 | 0.3121 | 0.4803 | 0.3044 | 0.2935 | 0.359 | 0.2803 | 0.1641 | 0.383 | 0.5238 | 0.3629 | 0.4919 | 0.4815 | 0.5601 | 0.4205 |
Nucleus | 0.5012 | 0.5295 | 0.46 | 0.5538 | 0.592 | 0.5004 | 0.5654 | 0.7035 | 0.5943 | 0.5831 | 0.7303 | 0.5928 | 0.4723 | 0.6244 | 0.4807 | 0.5154 | 0.4232 | 0.5708 |
Peroxisomes | 0.0099 | 0 | 0.004 | 0.0006 | 0.004 | 0.0001 | 0.0044 | 0 | 0.0002 | 0.0157 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0062 | 0.0102 | 0.0042 | 0.0018 | 0.0142 | 0.003 | 0.0185 | 0.0007 | 0.0092 | 0.0073 | 0.0117 | 0.01 | 0.0016 | 0.0012 | 0.003 | 0.0011 | 0.0022 | 0.001 |
Vacuole | 0.0031 | 0.0009 | 0.0036 | 0.0025 | 0.0051 | 0.001 | 0.0041 | 0.0005 | 0.0021 | 0.0017 | 0.0028 | 0.0011 | 0.0004 | 0.0067 | 0.0016 | 0.0002 | 0.0028 | 0.0018 |
Vacuole Periphery | 0.0029 | 0.0002 | 0.0012 | 0.0028 | 0.0031 | 0.0004 | 0.002 | 0.0002 | 0.0008 | 0.0016 | 0.0056 | 0.0005 | 0.0001 | 0.001 | 0.0003 | 0.0001 | 0.0009 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 46.6176 | 42.0014 | 38.4716 | 42.5413 | 40.6911 | 31.1835 | 48.7713 | 40.8668 | 36.4692 | 43.226 |
Translational Efficiency | 1.077 | 1.4237 | 1.335 | 0.9818 | 1.0604 | 1.4108 | 1.0551 | 0.9639 | 1.0303 | 0.9579 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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