Standard name
Human Ortholog
Description Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.19 0.19 0.07 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.28 0.16 0.35 0.36 0.27 0.5 0.48 0.47 0.51 0 0 0.05 0 0 0 0 0 0 0 0 0
Nucleus 0.53 0.42 0.6 0.77 0.78 0.7 0.65 0.68 0.69 0.62 0.65 0.65 0.63 0.56 0.56 0.7 0.68 0.68 0.31 0.33 0.32 0.38 0.24 0.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.38 0.43 0.28 0.11 0 0.05 0.08 0.13 0.06 0.09 0.09 0.14 0.2 0.2 0.18 0.12 0.12 0.15 0.55 0.48 0.42 0.33 0.34 0.33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
Vac/Vac Membrane 0.14 0.17 0.06 0 0.11 0.1 0.07 0.06 0 0.05 0 0 0.07 0.08 0.07 0.13 0.14 0.14 0 0.06 0.06 0.19 0.28 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0
Bud 1 0 3 0 2 2 6 8 9 11 8 8 0 0 1 0 0 0 0 7 6 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 1 16 10 15 5 12 5 12 0 0 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 10 14 8 9 17 24 29 16 23 13 9 36 63 71 21 11 12 0 1 0 2 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 5 6 1 0 1 1 0 2 0
Endosome 2 8 10 1 0 1 3 0 0 0 0 0 1 5 12 2 0 1 5 11 33 4 14 25
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 3 0 2 0 0 3 2
Mitochondria 1 5 50 92 68 161 221 196 197 247 200 214 2 5 17 8 0 5 0 0 2 2 3 5
Nucleus 65 103 240 252 335 326 402 499 272 318 276 271 146 189 209 214 99 131 49 108 155 69 80 68
Nuclear Periphery 0 1 1 0 4 2 8 2 3 0 9 7 0 2 1 3 0 1 1 0 6 0 2 6
Nucleolus 46 106 114 37 10 23 47 94 24 47 39 59 47 66 66 36 18 29 87 156 202 61 111 114
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 7 2 0 1 3 2 0 2 0 1 0 0 1 0 2 0 5 14 37 2 13 15
Vac/Vac Membrane 17 43 25 13 49 48 43 45 13 25 13 15 17 27 27 39 21 28 5 17 30 34 92 95
Unique Cell Count 122 246 403 327 429 464 614 735 392 513 427 417 233 335 370 306 146 194 159 326 483 184 331 345
Labelled Cell Count 135 278 464 407 478 597 767 890 539 685 563 597 249 359 411 333 160 211 159 326 483 184 331 345


Cytoplasm, Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 10.8 6.8 6.6 6.3 5.9 5.6 6.0 5.5 5.4 5.3 5.2 11.2 10.6 9.4 14.9 15.8 16.0 11.7 12.5 12.8
Std Deviation (1e-4) 2.4 2.1 1.2 1.2 1.0 1.0 1.0 1.1 1.3 1.1 1.1 1.3 2.6 2.7 2.3 3.0 3.6 3.6 2.1 2.3 2.4
Intensity Change (Log2) -0.05 -0.11 -0.22 -0.29 -0.2 -0.3 -0.34 -0.38 -0.38 0.71 0.63 0.45 1.13 1.2 1.23 0.77 0.87 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.8 1.7 1.4 1.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.8 0 2.9 0 3.1 0 3.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -1.2 0.2 0.4 0.4 0.4 0.8 -0.3 -1.1 5.4 6.8 7.0 2.1 2.0 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 -3.0 -2.8 0 0 0 0 0 0 -0.9 0.6 -1.9 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.3 1.4 7.6 8.3 5.6 11.5 11.5 10.8 11.9 -5.1 -5.6 -3.9 -4.7 -4.5 -3.9
Nucleus 5.0 5.8 3.3 1.9 2.8 2.9 0.7 1.5 1.6 0.8 -0.9 -0.9 2.9 1.8 1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -5.6 -10.5 -9.4 -8.8 -6.5 -8.2 -7.5 -7.1 -5.0 -2.3 -2.7 -3.4 -5.3 -3.9 -3.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 2.6 2.2 0.5 -0.1 -1.9 -0.9 -2.2 -1.7 0.5 1.0 0.6 3.0 3.1 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.944 10.8986 9.4992 9.8479 8.4408 10.0471 10.1277 14.2913 11.6616 10.8933 10.3081 12.271 13.0156 13.0718 12.5164 11.941 12.3365 12.3436
Actin 0.0202 0 0.0073 0.033 0.0047 0.0027 0.0068 0 0.0041 0.0221 0.0032 0.0003 0 0 0.0003 0 0.0001 0
Bud 0.0006 0 0.0003 0.001 0.001 0.0002 0.0006 0 0.0003 0.0004 0.0002 0.0001 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0032 0.0007 0.0013 0.0012 0.0131 0.0017 0.0013 0.0003 0.0011 0.0005 0.0006 0.0012 0.0004 0.0006 0.0006 0.0004 0.001 0.0019
Bud Periphery 0.0011 0.0001 0.0005 0.0014 0.0017 0.0004 0.0008 0.0001 0.0005 0.0006 0.0004 0.0002 0.0002 0.0002 0.0003 0.0001 0.0005 0.0003
Bud Site 0.0027 0.0002 0.0051 0.001 0.004 0.0004 0.0119 0.0001 0.0023 0.0015 0.0007 0.0003 0.0003 0.0011 0.0007 0.0002 0.0006 0.0002
Cell Periphery 0.0004 0.0002 0.0009 0.0007 0.0007 0.0002 0.0004 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002
Cytoplasm 0.002 0.0001 0.0007 0.0001 0.0021 0.0004 0.0054 0.0001 0.0015 0.002 0.0049 0.0005 0 0.0005 0.0028 0.0001 0.0002 0
Cytoplasmic Foci 0.0095 0 0.0067 0.0002 0.0063 0.0008 0.0099 0 0.0021 0.008 0.0021 0.0004 0 0 0.0019 0 0.0002 0
Eisosomes 0.0003 0 0.0002 0.0008 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0025 0 0.001 0.0002 0.0026 0.0007 0.0022 0 0.0009 0.0029 0.0046 0.0003 0 0 0.0031 0 0.0002 0
Endosome 0.0183 0.0001 0.005 0.0006 0.0063 0.0011 0.0099 0 0.0039 0.0176 0.0104 0.0019 0.0001 0.0001 0.0037 0 0.0015 0.0004
Golgi 0.0043 0 0.006 0.0007 0.0016 0.0006 0.0042 0 0.0023 0.0068 0.0009 0.0001 0 0 0.0004 0 0.0002 0
Lipid Particles 0.0108 0.0001 0.0129 0.0009 0.0044 0.0019 0.0139 0.0001 0.0022 0.0244 0.0043 0.0005 0.0001 0 0.0037 0 0.001 0.0003
Mitochondria 0.0101 0.0127 0.0062 0.0048 0.0052 0.0006 0.0034 0.0002 0.0029 0.0045 0.016 0.0005 0.0002 0.0001 0.0005 0.0001 0.0006 0.0002
None 0.0042 0 0.0004 0.0002 0.0034 0.0004 0.0152 0 0.0017 0.0026 0.0024 0.0006 0 0 0.0005 0 0.0001 0
Nuclear Periphery 0.0213 0.0014 0.0043 0.0177 0.0121 0.0028 0.0149 0.0006 0.0081 0.016 0.034 0.0056 0.0002 0.0007 0.0034 0.0005 0.0041 0.0015
Nucleolus 0.3651 0.4435 0.4681 0.3741 0.3121 0.4803 0.3044 0.2935 0.359 0.2803 0.1641 0.383 0.5238 0.3629 0.4919 0.4815 0.5601 0.4205
Nucleus 0.5012 0.5295 0.46 0.5538 0.592 0.5004 0.5654 0.7035 0.5943 0.5831 0.7303 0.5928 0.4723 0.6244 0.4807 0.5154 0.4232 0.5708
Peroxisomes 0.0099 0 0.004 0.0006 0.004 0.0001 0.0044 0 0.0002 0.0157 0.0005 0 0 0 0.0001 0 0.0001 0
Punctate Nuclear 0.0062 0.0102 0.0042 0.0018 0.0142 0.003 0.0185 0.0007 0.0092 0.0073 0.0117 0.01 0.0016 0.0012 0.003 0.0011 0.0022 0.001
Vacuole 0.0031 0.0009 0.0036 0.0025 0.0051 0.001 0.0041 0.0005 0.0021 0.0017 0.0028 0.0011 0.0004 0.0067 0.0016 0.0002 0.0028 0.0018
Vacuole Periphery 0.0029 0.0002 0.0012 0.0028 0.0031 0.0004 0.002 0.0002 0.0008 0.0016 0.0056 0.0005 0.0001 0.001 0.0003 0.0001 0.0009 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.6176 42.0014 38.4716 42.5413 40.6911 31.1835 48.7713 40.8668 36.4692 43.226
Translational Efficiency 1.077 1.4237 1.335 0.9818 1.0604 1.4108 1.0551 0.9639 1.0303 0.9579

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress
Localization
Cell Percentages nucleus (48%), nucleolus (15%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dis3

Dis3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dis3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available