Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 2 | 4 | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 3 | 2 | 6 |
Bud Neck | 0 | 0 | 1 | 15 | 10 | 8 | 13 | 16 | 7 | 4 | 6 | 7 | 4 | 31 | 16 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 4 | 5 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 3 | 13 | 9 | 4 | 19 | 10 | 20 | 0 | 1 | 1 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 3 | 2 | 6 | 5 | 8 | 5 | 7 | 2 | 13 | 7 | 12 | 19 | 57 | 76 | 13 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 2 | 0 | 1 | 4 | 16 | 8 | 41 | 15 | 10 | 13 | 8 | 8 | 2 | 12 | 23 | 4 | 0 | 1 | 5 | 2 | 4 | 2 | 10 | 32 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 4 |
Mitochondria | 35 | 12 | 9 | 6 | 11 | 32 | 55 | 32 | 39 | 59 | 41 | 78 | 22 | 10 | 8 | 40 | 13 | 13 | 1 | 0 | 1 | 3 | 5 | 5 |
Nucleus | 121 | 52 | 44 | 10 | 10 | 7 | 15 | 21 | 14 | 12 | 13 | 29 | 19 | 18 | 13 | 37 | 27 | 21 | 76 | 51 | 22 | 15 | 17 | 15 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 1 | 1 | 2 | 5 |
Nucleolus | 360 | 127 | 146 | 274 | 293 | 281 | 503 | 481 | 273 | 275 | 208 | 354 | 240 | 467 | 464 | 299 | 166 | 104 | 403 | 130 | 152 | 216 | 287 | 258 |
Peroxisomes | 1 | 0 | 0 | 6 | 9 | 23 | 51 | 29 | 5 | 16 | 10 | 21 | 1 | 12 | 14 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 6 |
SpindlePole | 1 | 1 | 1 | 44 | 92 | 100 | 229 | 244 | 74 | 98 | 66 | 133 | 8 | 35 | 51 | 3 | 1 | 2 | 11 | 3 | 6 | 12 | 72 | 53 |
Vac/Vac Membrane | 15 | 19 | 5 | 0 | 2 | 1 | 5 | 2 | 1 | 3 | 1 | 1 | 5 | 10 | 7 | 2 | 0 | 1 | 16 | 9 | 8 | 6 | 7 | 26 |
Unique Cell Count | 472 | 194 | 190 | 294 | 318 | 317 | 578 | 580 | 293 | 329 | 248 | 445 | 277 | 554 | 570 | 338 | 188 | 121 | 532 | 205 | 206 | 265 | 421 | 432 |
Labelled Cell Count | 542 | 214 | 210 | 366 | 450 | 472 | 936 | 860 | 434 | 514 | 372 | 667 | 321 | 653 | 673 | 404 | 212 | 146 | 532 | 205 | 206 | 265 | 421 | 432 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.0 | 19.8 | 17.0 | 16.9 | 13.6 | 10.2 | 9.1 | 9.2 | 9.2 | 8.3 | 8.3 | 8.5 | 19.2 | 18.4 | 16.7 | 25.1 | 29.5 | 31.5 | 20.0 | 18.7 | 19.5 |
Std Deviation (1e-4) | 4.7 | 4.6 | 4.0 | 4.2 | 3.3 | 2.6 | 2.6 | 2.4 | 2.2 | 2.7 | 2.5 | 2.6 | 5.7 | 6.6 | 6.3 | 8.6 | 12.0 | 12.0 | 4.6 | 4.3 | 4.9 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.32 | -0.74 | -0.9 | -0.89 | -0.89 | -1.04 | -1.04 | -1.0 | 0.17 | 0.11 | -0.03 | 0.56 | 0.79 | 0.89 | 0.23 | 0.14 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 3.0 | 4.1 | 4.8 | 1.9 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.7 | 0 | 3.5 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.7 | -0.7 | 2.1 | 2.1 | 0.4 | 3.1 | 4.3 | 3.8 | 4.3 | 1.4 | 0 | 0 | 2.7 | 0.9 | 2.0 |
Nucleus | -6.7 | -7.1 | -7.6 | -9.2 | -8.4 | -6.1 | -6.9 | -5.5 | -6.0 | -5.1 | -8.6 | -9.5 | -3.7 | -2.2 | -1.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5.2 | 4.9 | 3.5 | 3.4 | 1.9 | 5.2 | 1.9 | 1.9 | 0.8 | 2.7 | 2.3 | 1.4 | 3.5 | 2.9 | 2.0 |
Peroxisomes | 0 | 0 | 3.8 | 4.2 | 3.1 | 0 | 3.1 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5.3 | 8.0 | 8.5 | 10.2 | 10.7 | 7.3 | 8.2 | 7.5 | 8.3 | 0 | 3.2 | 4.0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.2207 | 16.7337 | 15.6223 | 15.4559 | 15.0647 | 15.1152 | 13.0564 | 16.1769 | 13.9097 | 12.9509 | 13.6273 | 13.9746 | 16.8355 | 21.4501 | 19.475 | 18.0858 | 18.5624 | 18.8344 |
Actin | 0.0125 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0164 | 0.0084 | 0.0168 | 0.0747 | 0.0361 | 0.0018 | 0.0065 | 0 | 0.0022 | 0.0005 | 0.0004 | 0.0077 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Bud Neck | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0009 | 0.0005 | 0.0002 | 0.0005 | 0.0007 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0017 | 0.0003 |
Bud Periphery | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0013 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0014 | 0.0001 |
Bud Site | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0013 | 0.0012 | 0.0005 | 0.0017 | 0.0002 | 0.0011 | 0.0001 | 0.0015 | 0.0003 | 0.0007 | 0.0001 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0019 | 0.0002 |
Cytoplasm | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0016 | 0.0009 | 0.0001 | 0.0002 | 0.0007 | 0.0079 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 |
Cytoplasmic Foci | 0.0043 | 0.0012 | 0.0021 | 0.0001 | 0.0013 | 0.0023 | 0.0078 | 0.0025 | 0.0121 | 0.023 | 0.0084 | 0.0111 | 0.016 | 0.0049 | 0.0068 | 0.0109 | 0.0055 | 0.0008 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0021 | 0.0022 | 0.0013 | 0 | 0.0003 | 0.0002 | 0.0086 | 0 | 0.0006 | 0.0001 | 0.0001 | 0 |
Endosome | 0.0011 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0011 | 0.0038 | 0.0141 | 0.0263 | 0.0007 | 0.0051 | 0.008 | 0.0146 | 0 | 0.0023 | 0.0006 | 0.001 | 0.0002 |
Golgi | 0.0016 | 0.0001 | 0.0003 | 0 | 0.0004 | 0.0005 | 0.0026 | 0.0033 | 0.0101 | 0.0028 | 0.0107 | 0.0074 | 0.0052 | 0 | 0.0004 | 0.0004 | 0.0011 | 0.0002 |
Lipid Particles | 0.0089 | 0.0024 | 0.0057 | 0.0002 | 0.029 | 0.0118 | 0.0232 | 0.0049 | 0.0156 | 0.068 | 0.0235 | 0.0271 | 0.0085 | 0.0021 | 0.0047 | 0.0154 | 0.0279 | 0.0114 |
Mitochondria | 0.0015 | 0.0001 | 0.0004 | 0.0001 | 0.001 | 0.0016 | 0.0032 | 0.0113 | 0.012 | 0.0003 | 0.0074 | 0.0017 | 0.0043 | 0.0025 | 0.0058 | 0.0089 | 0.0065 | 0.001 |
None | 0.0007 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0.0011 | 0.0003 | 0.001 | 0.0001 | 0.0005 | 0.0006 | 0.0071 | 0 | 0.0008 | 0.0004 | 0.0006 | 0.0001 |
Nuclear Periphery | 0.0113 | 0.0004 | 0.0007 | 0.0002 | 0.0133 | 0.0045 | 0.0055 | 0.0264 | 0.0076 | 0.0001 | 0.0483 | 0.0079 | 0.013 | 0.0005 | 0.0028 | 0.0292 | 0.0118 | 0.0096 |
Nucleolus | 0.8514 | 0.8417 | 0.9157 | 0.9026 | 0.8735 | 0.8732 | 0.7774 | 0.7806 | 0.8108 | 0.6695 | 0.7178 | 0.8087 | 0.7963 | 0.8625 | 0.8983 | 0.8353 | 0.7881 | 0.8466 |
Nucleus | 0.0866 | 0.1409 | 0.0662 | 0.0946 | 0.0713 | 0.0946 | 0.1366 | 0.1284 | 0.0699 | 0.1488 | 0.0943 | 0.1107 | 0.0811 | 0.1249 | 0.0678 | 0.0828 | 0.1274 | 0.1156 |
Peroxisomes | 0.0024 | 0.0001 | 0.0018 | 0 | 0.0003 | 0.0004 | 0.0079 | 0.0029 | 0.0054 | 0.0072 | 0.0116 | 0.0032 | 0.0047 | 0.0006 | 0.0004 | 0.0018 | 0.0006 | 0.0001 |
Punctate Nuclear | 0.0155 | 0.0117 | 0.0026 | 0.0011 | 0.0064 | 0.0065 | 0.0073 | 0.0059 | 0.0035 | 0.0024 | 0.023 | 0.009 | 0.0152 | 0.0008 | 0.0035 | 0.0031 | 0.0113 | 0.0039 |
Vacuole | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0009 | 0.001 | 0.0026 | 0.0021 | 0.0003 | 0.0016 | 0.0004 | 0.0025 | 0.0004 | 0.0004 | 0.0031 | 0.0045 | 0.0006 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0022 | 0.0007 | 0.0012 | 0.0023 | 0.002 | 0.0001 | 0.0062 | 0.0007 | 0.0061 | 0.0004 | 0.0004 | 0.006 | 0.0066 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 80.7564 | 60.0403 | 62.0552 | 56.8567 | 71.5306 | 49.5458 | 52.9377 | 43.1679 | 46.2123 | 57.3447 |
Translational Efficiency | 2.5596 | 3.1679 | 2.4738 | 2.6443 | 2.2422 | 2.5283 | 1.8904 | 2.2602 | 1.7335 | 2.1892 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|