Standard name
Human Ortholog
Description PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.97 0.98 0.89 0.88 0.85 0.84 – 0.73 0.77 1.0 0.99 0.97 0.91 0.93 0.94 0.97 0.94 0.91 0.93 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.22 0.07 0.27 0.29 0.32 0.42 – 0.51 0.54 0 0 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 0 0 1 0 0 1
Bud 1 0 2 2 0 3 7 13 5 – 13 8 0 0 4 0 0 1 0 0 0 1 2 2
Bud Neck 0 0 0 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 1 1 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 8 7 7 3 6 4 8 4 3 – 6 8 1 3 4 11 15 21 0 0 1 0 0 0
Cytoplasm 225 272 365 310 319 359 431 509 259 – 219 273 162 186 182 286 262 312 221 269 359 104 160 182
Endoplasmic Reticulum 1 1 3 0 0 1 3 1 0 – 1 0 0 1 0 12 7 15 0 0 4 0 1 4
Endosome 0 0 1 0 0 0 1 5 0 – 0 0 0 0 1 6 2 1 0 2 1 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 1 0 0 1
Mitochondria 0 2 18 69 22 110 144 194 131 – 153 191 1 0 20 10 1 8 2 3 15 1 1 2
Nucleus 2 1 0 0 2 2 2 4 1 – 2 1 0 0 0 5 6 3 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 1 1 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 – 0 0 0 0 0 3 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 2 2 – 0 1 0 0 0 2 0 1 1 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 2 0 6 3 0 – 0 2 0 0 1 25 18 12 0 3 3 1 2 2
Unique Cell Count 226 274 376 320 327 403 489 598 310 298 356 162 187 188 316 282 331 228 287 394 113 175 206
Labelled Cell Count 237 284 399 384 351 481 602 738 402 396 486 164 191 213 362 312 374 228 287 394 113 175 206


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 5.8 4.4 4.5 5.0 4.2 4.0 4.1 3.6 – 3.7 3.4 5.8 6.0 4.9 8.8 9.0 9.0 5.7 6.3 7.1
Std Deviation (1e-4) 0.7 0.7 0.6 0.9 1.4 0.9 1.0 1.3 1.0 – 5.4 1.0 0.6 1.1 1.5 1.3 1.4 1.3 1.1 1.4 1.4
Intensity Change (Log2) – – – 0.03 0.18 -0.07 -0.14 -0.1 -0.31 – -0.26 -0.4 0.4 0.43 0.14 0.99 1.03 1.02 0.37 0.5 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1.3 2.4 3.0
Cytoplasm -0.2 0.4 -4.4 -4.8 -6.0 -6.1 – -8.9 -8.2 2.2 1.8 -0.2 -3.6 -2.5 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.7 0 3.1
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 1.1 0 0 0 0 – 0 0 -2.4 -3.0 2.6 -1.1 -3.4 -1.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 5.0 4.3 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2091 2.7759 3.1222 2.4928 3.0744 2.6166 3.7669 3.8487 3.9963 4.1533 4.4979 3.9121 3.995 4.9842 4.838 4.4398 3.9018 4.5835
Actin 0.0764 0.0004 0.0646 0.002 0.0689 0.0006 0.064 0.0087 0.01 0.0039 0.0139 0.0079 0.0175 0.0022 0.0057 0.0037 0.0098 0.0043
Bud 0.0015 0.0004 0.008 0.0006 0.0023 0.0009 0.0049 0.0016 0.0032 0.003 0.0011 0.002 0.0002 0.0025 0.0016 0.0002 0.0004 0.0006
Bud Neck 0.0092 0.0003 0.0048 0.0014 0.0361 0.0006 0.0096 0.0006 0.0002 0.0004 0.001 0.0033 0.0003 0.0002 0.0003 0.0002 0.0003 0.0079
Bud Periphery 0.0014 0.0003 0.0029 0.0005 0.0049 0.0006 0.0046 0.002 0.0046 0.0049 0.0014 0.0025 0.0003 0.0042 0.0017 0.0002 0.0004 0.0008
Bud Site 0.0089 0.0067 0.026 0.0003 0.0105 0.0002 0.0124 0.0163 0.0019 0.0021 0.0039 0.001 0.001 0.0036 0.0026 0.0001 0.0003 0.0019
Cell Periphery 0.0036 0.0025 0.0007 0.0007 0.0007 0.0002 0.0007 0.002 0.0003 0.0004 0.0001 0.0002 0.0003 0.0004 0.0003 0.0001 0.0001 0.0003
Cytoplasm 0.615 0.8366 0.7023 0.8506 0.6276 0.8464 0.5293 0.8569 0.7646 0.616 0.5441 0.8144 0.7073 0.8245 0.8024 0.874 0.7221 0.8474
Cytoplasmic Foci 0.063 0.0078 0.0192 0.0125 0.009 0.0079 0.0521 0.0082 0.0146 0.0306 0.0327 0.0137 0.0186 0.0056 0.0142 0.0044 0.0169 0.0063
Eisosomes 0.0005 0.0001 0.0006 0 0.0006 0 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0013 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0097 0.007 0.0071 0.0608 0.0079 0.0043 0.0103 0.0054 0.0042 0.0098 0.0039 0.0051 0.0032 0.0008 0.0044 0.0043 0.0025 0.0053
Endosome 0.0176 0.0031 0.0087 0.026 0.0205 0.0047 0.0509 0.0057 0.0273 0.0446 0.0457 0.016 0.0203 0.0021 0.0075 0.0192 0.0196 0.0054
Golgi 0.0129 0.0006 0.0074 0.003 0.0064 0.0024 0.0208 0.0009 0.002 0.017 0.0318 0.0064 0.0072 0.0003 0.0026 0.0087 0.0325 0.0013
Lipid Particles 0.0108 0.0006 0.0036 0.0044 0.0405 0.0051 0.0169 0.0002 0.002 0.01 0.0208 0.002 0.012 0 0.0039 0.0036 0.0005 0.0001
Mitochondria 0.0021 0.0002 0.0019 0.0005 0.0265 0.0143 0.0161 0.0006 0.0015 0.0098 0.0208 0.005 0.0012 0.0003 0.0014 0.0618 0.005 0.0027
None 0.0937 0.1292 0.1134 0.0196 0.07 0.1075 0.1505 0.0843 0.1416 0.2142 0.2303 0.1106 0.1916 0.151 0.1429 0.0091 0.1863 0.1128
Nuclear Periphery 0.0037 0.0005 0.007 0.0034 0.0379 0.0002 0.014 0.0011 0.0034 0.006 0.0034 0.0035 0.0032 0.0001 0.001 0.0008 0.0003 0.0003
Nucleolus 0.0009 0.0001 0.0006 0.0001 0.0014 0.0001 0.0016 0.0001 0.0001 0.0003 0.0006 0.0001 0.0004 0 0.0001 0 0 0
Nucleus 0.0015 0.0007 0.007 0.0023 0.0077 0.0004 0.0078 0.0011 0.0015 0.0031 0.0011 0.0012 0.0007 0.0005 0.0008 0.0005 0.0004 0.0004
Peroxisomes 0.0199 0.0001 0.0044 0.0001 0.0077 0.0009 0.02 0.0002 0.0075 0.0148 0.0348 0.0014 0.0084 0.0001 0.0024 0.0009 0.0009 0.0001
Punctate Nuclear 0.0433 0.0002 0.0069 0.0005 0.0018 0.0002 0.0054 0.0004 0.0013 0.0018 0.0027 0.0009 0.0031 0.0001 0.0009 0.0001 0.0003 0.0001
Vacuole 0.004 0.0027 0.0025 0.0097 0.0057 0.002 0.0056 0.0032 0.0071 0.0059 0.0047 0.002 0.0023 0.0013 0.0018 0.0058 0.0009 0.0016
Vacuole Periphery 0.0006 0.0001 0.0004 0.0009 0.0054 0.0004 0.002 0.0004 0.0012 0.0014 0.0011 0.0006 0.0007 0.0002 0.0003 0.0021 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3618 15.813 16.3833 15.9371 21.4202 15.3444 21.7203 18.7319 18.3867 24.8597
Translational Efficiency 0.7944 0.833 0.5868 0.4729 0.4738 0.8775 0.5597 0.457 0.3973 0.4775

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Psk2

Psk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Psk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available