Standard name
Human Ortholog
Description Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.06 0.05 0 0 0 – 0 0
Cytoplasm 0.95 0.98 0.9 0.98 0.98 0.94 0.9 0.91 0.74 0.75 0.61 0.7 – – – 0.96 0.97 0.98 0.88 0.89 0.73 – 0.75 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0.12 0
Mitochondria 0 0 0.05 0 0 0.1 0.1 0.09 0.35 0.22 0.52 0.39 – – – 0 0 0 0 0 0.11 – 0 0.07
Nucleus 0 0 0.08 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 2 0 1 0 0 – – – 0 0 0 0 1 0 – 0 0
Bud 1 1 0 1 0 0 2 2 4 2 5 4 – – – 0 0 0 1 8 0 – 0 0
Bud Neck 0 0 0 2 1 0 0 6 3 1 0 1 – – – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 4 0 2 4 6 3 4 2 5 2 2 – – – 6 18 14 0 0 0 – 0 0
Cytoplasm 142 299 35 113 183 164 209 328 116 126 117 124 – – – 299 311 294 132 267 49 – 5 12
Endoplasmic Reticulum 2 4 0 0 0 1 2 2 1 4 2 0 – – – 12 11 9 1 1 1 – 0 0
Endosome 0 0 0 0 0 0 0 0 1 1 0 4 – – – 1 0 1 3 2 1 – 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 0 2 0 – 0 0
Mitochondria 6 1 2 0 1 18 22 32 54 37 100 69 – – – 7 4 1 4 4 7 – 0 1
Nucleus 0 1 3 0 1 1 0 5 3 4 0 0 – – – 2 5 4 0 0 1 – 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 0 1 1 – – – 1 0 0 1 3 0 – 0 0
Vac/Vac Membrane 0 2 0 1 2 1 4 4 4 7 4 7 – – – 8 4 2 0 3 0 – 0 0
Unique Cell Count 150 306 39 115 186 174 231 362 156 168 192 178 312 320 301 150 299 68 8 16
Labelled Cell Count 152 315 40 119 192 192 244 386 188 188 231 212 337 354 325 150 299 68 8 16


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.6 5.8 6.5 6.8 5.6 5.7 5.6 4.6 4.8 4.1 5.1 – – – 7.9 8.0 7.7 – – –
Std Deviation (1e-4) 1.5 2.1 1.5 1.7 2.0 2.2 1.8 2.0 1.8 1.6 1.1 2.1 – – – 1.5 1.4 1.3 – – –
Intensity Change (Log2) – – – 0.16 0.23 -0.05 -0.03 -0.05 -0.33 -0.28 -0.49 -0.19 – – – 0.44 0.47 0.41 – – –

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT302468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT102468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 2.4 2.8 1.0 0.1 0.2 -2.1 -2.0 -3.5 -2.6 – – – 1.7 2.3 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 4.1 – – – 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4992 2.0553 2.0177 1.6238 1.7217 1.7074 0.6987 1.2627 1.105 1.1125 0.3571 1.0582 -0.1951 1.1734 0.221 -0.2205 1.0574 0.7908
Actin 0.0273 0.0017 0.0044 0.0008 0.0406 0.0015 0.0202 0.0006 0.0088 0.0003 0.0007 0.0081 0.023 0.0007 0.0048 0.0005 0.0614 0.0158
Bud 0.0121 0.0084 0.0029 0.0034 0.0016 0.0031 0.0004 0.0002 0.0012 0.0005 0.0012 0.0005 0.0002 0.0038 0.0002 0.0001 0.0015 0.0003
Bud Neck 0.0021 0.0003 0.0006 0.0048 0.0011 0.0005 0.0006 0.0002 0.002 0.0003 0.0005 0.0006 0.0008 0.0002 0.0013 0.0001 0.0052 0.0005
Bud Periphery 0.006 0.0013 0.0012 0.0025 0.0028 0.0018 0.0006 0.0002 0.0027 0.001 0.0069 0.0005 0.0002 0.0031 0.0001 0 0.0019 0.0005
Bud Site 0.0144 0.0081 0.0022 0.0021 0.0019 0.0002 0.0026 0.0043 0.0073 0.0003 0.0011 0.0002 0.0033 0.0079 0.0011 0.0001 0.0073 0.0016
Cell Periphery 0.0007 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0002
Cytoplasm 0.4227 0.7052 0.6655 0.4483 0.5308 0.707 0.3735 0.5227 0.4559 0.5659 0.3406 0.5845 0.4533 0.5759 0.6062 0.4897 0.4562 0.7138
Cytoplasmic Foci 0.0412 0.0088 0.0118 0.0506 0.0513 0.0142 0.0466 0.0051 0.015 0.0092 0.011 0.0078 0.028 0.0061 0.0112 0.006 0.0517 0.0035
Eisosomes 0.0004 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0001 0 0.0002 0.0002 0.0003 0 0.0001 0 0.0013 0.0002
Endoplasmic Reticulum 0.0043 0.0017 0.0024 0.0022 0.003 0.0021 0.0022 0.001 0.0025 0.0022 0.002 0.0015 0.0039 0.0022 0.0031 0.0073 0.0035 0.001
Endosome 0.0199 0.0035 0.0106 0.0088 0.0486 0.0064 0.0295 0.0009 0.0089 0.0405 0.0248 0.0025 0.0125 0.0036 0.0043 0.0046 0.073 0.0015
Golgi 0.0063 0.0003 0.0032 0.002 0.0167 0.0007 0.0082 0.0002 0.0046 0.0051 0.0018 0.0012 0.0023 0.0004 0.0034 0.0003 0.0514 0.0007
Lipid Particles 0.017 0.0001 0.0033 0.0015 0.0124 0.0008 0.015 0.0001 0.0103 0.0004 0.0277 0.0007 0.0031 0.0003 0.0077 0.0002 0.0389 0.0009
Mitochondria 0.0221 0.0005 0.015 0.0057 0.0126 0.0008 0.0058 0.0002 0.0046 0.0072 0.0099 0.002 0.0008 0.0148 0.0008 0.0001 0.036 0.0008
None 0.3414 0.255 0.2648 0.4472 0.2208 0.256 0.4473 0.4615 0.4594 0.3429 0.5365 0.3842 0.4579 0.3778 0.3336 0.4879 0.1364 0.233
Nuclear Periphery 0.0106 0.0005 0.0011 0.001 0.0046 0.0009 0.0064 0.0003 0.0025 0.0008 0.0111 0.0008 0.0013 0.0004 0.0007 0.0008 0.0061 0.0015
Nucleolus 0.0022 0.0001 0.0009 0.001 0.0008 0.0002 0.0007 0.0001 0.0002 0.0001 0.0035 0.0001 0.0005 0.0001 0.0001 0 0.0006 0.0006
Nucleus 0.0044 0.0018 0.0036 0.0027 0.0048 0.0012 0.0018 0.0009 0.0018 0.0013 0.0028 0.0009 0.0011 0.0005 0.0007 0.0005 0.0016 0.02
Peroxisomes 0.0285 0.0002 0.0014 0.0108 0.0193 0.0004 0.0198 0.0002 0.0084 0.0002 0.0043 0.0008 0.0022 0.0006 0.0188 0 0.0547 0.002
Punctate Nuclear 0.0109 0.0006 0.001 0.0021 0.0233 0.0007 0.0158 0.0003 0.0013 0.0003 0.0067 0.0024 0.003 0.0004 0.0005 0.0003 0.0045 0.0004
Vacuole 0.0042 0.0012 0.0025 0.0017 0.0019 0.0011 0.0019 0.0005 0.0015 0.0131 0.0047 0.0004 0.0018 0.001 0.0011 0.0011 0.0036 0.0009
Vacuole Periphery 0.0014 0.0002 0.0011 0.0004 0.001 0.0002 0.0008 0 0.0004 0.0082 0.0016 0.0002 0.0003 0.0001 0.0002 0.0002 0.0027 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.8428 11.1078 11.5395 13.5636 13.6939 10.556 14.1793 12.1284 11.6726 15.3923
Translational Efficiency 0.9585 1.057 1.1293 0.9493 0.9271 1.1737 0.7952 0.9917 1.0511 0.9033

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
829 1227 2335 1180 1644 1851 295 168 2473 3078 2630 1348

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 679.96 635.13 1093.82 959.23 711.62 800.45 1086.55 1068.31 701.01 734.55 1093.00 972.82
Standard Deviation 122.43 132.47 172.06 157.07 116.62 137.46 153.88 176.08 119.54 157.83 170.13 163.58
Intensity Change Log 2 — -0.098398 0.685854 0.496427 — 0.169704 0.610576 0.586151 — 0.044910 0.647849 0.543007

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000154 0.000229 0.000230 0.004300 0.000121 0.000316 0.000532 0.010366 0.000132 0.000281 0.000264 0.005056
Bud Neck 0.005924 0.032092 0.001367 0.005843 0.015708 0.019545 0.003838 0.006060 0.012428 0.024547 0.001645 0.005870
Bud Site 0.008026 0.020160 0.005475 0.060862 0.007462 0.024819 0.011972 0.049895 0.007651 0.022962 0.006204 0.059495
Cell Periphery 0.000220 0.000200 0.000040 0.000216 0.000191 0.000176 0.000109 0.000164 0.000200 0.000185 0.000048 0.000210
Cytoplasm 0.657161 0.479585 0.705359 0.663191 0.577910 0.589264 0.615881 0.523396 0.604477 0.545542 0.695323 0.645768
Cytoplasmic Foci 0.139989 0.222353 0.006644 0.022782 0.221477 0.144682 0.026643 0.060364 0.194161 0.175645 0.008887 0.027466
Eisosomes 0.000120 0.000136 0.000006 0.000029 0.000151 0.000073 0.000038 0.000067 0.000141 0.000098 0.000010 0.000034
Endoplasmic Reticulum 0.000470 0.001159 0.000626 0.001854 0.001047 0.000670 0.001555 0.001649 0.000854 0.000865 0.000731 0.001829
Endosome 0.006061 0.025362 0.000710 0.004281 0.011647 0.020559 0.004993 0.019280 0.009774 0.022474 0.001191 0.006150
Golgi 0.002254 0.007600 0.000099 0.003749 0.003539 0.004911 0.000166 0.004840 0.003108 0.005983 0.000107 0.003885
Lipid Particles 0.004867 0.008088 0.000335 0.000625 0.005218 0.002964 0.001247 0.002620 0.005101 0.005007 0.000437 0.000874
Mitochondria 0.001579 0.016253 0.000319 0.006293 0.004935 0.008194 0.000275 0.009071 0.003810 0.011407 0.000314 0.006639
Mitotic Spindle 0.003614 0.003378 0.005023 0.018052 0.000392 0.008975 0.006841 0.025571 0.001472 0.006744 0.005227 0.018989
None 0.017856 0.020608 0.007262 0.004126 0.005674 0.004263 0.009170 0.003959 0.009758 0.010779 0.007476 0.004106
Nuclear Periphery 0.000182 0.000372 0.000611 0.000443 0.000195 0.000212 0.000709 0.000692 0.000191 0.000276 0.000622 0.000474
Nuclear Periphery Foci 0.000567 0.000953 0.000776 0.001396 0.000383 0.000475 0.003867 0.003971 0.000444 0.000665 0.001123 0.001717
Nucleolus 0.000785 0.004850 0.000147 0.000146 0.000681 0.001647 0.000323 0.001103 0.000716 0.002924 0.000167 0.000266
Nucleus 0.119906 0.068492 0.236513 0.083509 0.084839 0.056176 0.110749 0.057552 0.096594 0.061086 0.222406 0.080274
Peroxisomes 0.003672 0.007005 0.000173 0.004051 0.003552 0.007039 0.000650 0.009831 0.003593 0.007025 0.000226 0.004771
Vacuole 0.025840 0.076314 0.028200 0.111019 0.052862 0.102336 0.199756 0.203118 0.043804 0.091963 0.047443 0.122498
Vacuole Periphery 0.000754 0.004810 0.000086 0.003230 0.002015 0.002704 0.000688 0.006431 0.001592 0.003543 0.000154 0.003629

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.17 -1.12 -3.34 -3.19 -3.16 -5.93 -2.29 -1.20 -1.15 -1.13 -4.97 -2.69 -3.11 -2.84 -2.88
Bud Neck -9.74 4.84 2.97 11.13 -2.72 -1.99 9.24 9.84 12.08 0.16 -7.69 11.82 9.80 15.86 -2.63
Bud Site -5.76 1.82 -4.98 -1.27 -6.45 -9.01 -0.58 -2.32 0.52 -2.07 -10.92 2.44 -5.99 -0.40 -6.78
Cell Periphery 0.96 9.97 3.77 3.24 -5.80 0.50 3.22 1.92 1.42 -0.96 0.72 8.06 3.00 2.43 -5.93
Cytoplasm 15.14 0.24 7.40 -7.62 8.66 -0.98 3.55 7.71 8.06 4.34 8.10 -5.00 5.83 -0.49 9.68
Cytoplasmic Foci -10.19 24.73 21.38 32.48 -14.08 12.17 36.05 30.71 21.10 -0.88 3.71 47.34 42.34 41.91 -13.08
Eisosomes -0.75 6.15 4.99 10.67 -14.33 7.35 10.69 8.10 2.34 -1.97 4.29 14.26 11.87 13.32 -12.87
Endoplasmic Reticulum -3.89 -3.48 -3.44 -0.24 -1.88 1.83 -1.92 -1.54 -2.39 -0.45 -0.03 -0.48 -1.99 -2.14 -1.97
Endosome -12.29 8.67 6.34 15.96 -3.43 -6.55 9.52 3.00 6.66 -0.76 -12.25 16.45 11.98 21.12 -3.13
Golgi -6.74 5.36 1.90 7.81 -1.97 -1.65 9.25 3.36 4.05 -1.17 -5.01 10.79 3.96 7.44 -2.17
Lipid Particles -2.91 5.90 5.16 8.96 -9.62 4.83 9.51 7.73 3.39 -1.24 0.25 12.60 11.03 10.77 -8.86
Mitochondria -7.68 3.43 -0.20 6.99 -1.67 -2.25 5.57 1.87 3.41 -0.98 -6.53 6.00 2.08 7.54 -1.85
Mitotic Spindle 0.22 -1.24 -3.35 -3.59 -2.77 -5.87 -3.52 -2.66 -1.39 -1.54 -5.09 -5.02 -5.48 -2.49 -3.18
None -0.84 5.39 6.74 7.03 5.36 1.43 0.32 2.53 0.98 1.57 -0.70 3.79 6.95 6.64 5.60
Nuclear Periphery -3.60 -17.04 -8.99 -5.15 4.44 -0.56 -11.32 -6.30 -6.02 1.03 -2.98 -23.72 -11.59 -9.03 4.48
Nuclear Periphery Foci -2.53 -5.76 -10.62 -4.98 -5.14 -1.33 -10.36 -4.98 -4.80 0.95 -3.00 -11.81 -12.97 -9.92 -3.54
Nucleolus -4.92 6.11 6.52 5.72 2.06 -2.84 3.43 1.91 3.67 -0.36 -5.68 6.87 7.08 7.17 0.95
Nucleus 8.20 -18.98 2.32 -7.15 25.13 7.59 -2.87 1.56 -1.80 3.33 10.78 -28.59 -0.91 -9.74 24.95
Peroxisomes -2.56 2.97 1.75 7.01 -1.96 -4.19 9.09 1.22 3.92 -2.23 -5.04 7.52 3.01 7.50 -2.39
Vacuole -13.15 -12.11 -28.31 -20.21 -22.72 -13.55 -16.19 -15.52 -13.20 -4.26 -17.56 -13.08 -29.51 -22.03 -21.81
Vacuole Periphery -8.07 4.46 -0.38 6.38 -2.17 -1.34 4.94 1.01 1.78 -1.23 -5.36 6.72 1.59 5.78 -2.43
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (62%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rib2

Rib2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rib2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available