Standard name
Human Ortholog
Description Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0.1 0.13 – – – 0 0 0 0.07 0 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.22 0.16 0.11 0.13 0.11 0.09 0.05 – 0.2 0.08 0.16 0.12 0.24 0.08 0.09 – – – 0 0 0 0 0.06 0.05
Nucleus 0.23 0.18 0.09 0 0.06 0.06 0.06 – 0.06 0.05 0.07 0.07 0 0 0 – – – 0.14 0.15 0.06 0.07 0 0.06
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.76 0.8 0.86 0.93 0.84 0.86 0.85 – 0.86 0.86 0.85 0.83 0.91 0.85 0.8 – – – 0.75 0.68 0.75 0.7 0.66 0.58
Peroxisomes 0 0 0 0 0 0.08 0.05 – 0 0.05 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0.09 0.12 0.18 0.24 – 0.16 0.22 0.16 0.23 0.07 0.12 0.08 – – – 0 0 0.07 0 0.06 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0.05 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 1 2 0 0 1
Bud 0 2 0 0 0 0 2 – 5 2 4 5 0 0 0 – – – 3 3 6 2 3 8
Bud Neck 0 1 6 3 2 1 5 – 2 7 2 4 0 8 1 – – – 0 2 2 1 2 0
Bud Site 1 0 0 0 0 3 4 – 17 2 12 41 0 2 3 – – – – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 1 0 3 – – – 0 0 0 0 0 1
Cytoplasm 11 2 9 8 9 2 7 – 7 6 4 10 15 36 49 – – – 0 7 0 16 7 21
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Endosome 0 3 3 2 12 23 56 – 9 27 34 38 2 3 5 – – – 0 7 9 2 7 9
Golgi 0 0 0 0 1 2 0 – 0 0 0 0 0 0 0 – – – 0 1 1 1 1 1
Mitochondria 93 70 63 52 49 56 47 – 123 53 152 108 52 28 36 – – – 5 14 20 5 14 13
Nucleus 97 80 52 8 28 33 51 – 38 36 62 64 4 16 12 – – – 60 72 34 17 9 16
Nuclear Periphery 1 2 2 0 3 5 4 – 6 4 5 4 0 1 0 – – – 15 7 11 2 4 7
Nucleolus 315 362 481 383 363 513 742 – 537 580 791 752 197 305 304 – – – 332 335 450 169 168 158
Peroxisomes 0 1 7 16 9 46 43 – 16 31 20 21 7 7 6 – – – 0 1 0 0 2 0
SpindlePole 1 1 24 35 53 110 211 – 98 146 151 213 16 42 29 – – – 3 12 40 9 16 9
Vac/Vac Membrane 22 12 14 3 9 6 15 – 0 7 9 9 1 1 2 – – – 19 22 11 7 11 15
Unique Cell Count 417 451 559 411 433 599 871 627 673 934 909 216 358 379 445 494 601 241 254 272
Labelled Cell Count 542 536 661 510 538 800 1187 858 901 1246 1269 295 449 450 445 494 601 241 254 272


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 35.0 23.5 24.1 23.1 14.9 15.7 14.5 – 12.7 13.7 12.7 12.4 25.8 20.2 21.9 – – – 29.0 28.3 27.5
Std Deviation (1e-4) 14.3 9.1 8.4 8.7 5.2 5.5 4.7 – 4.8 4.5 4.1 4.7 11.3 10.3 10.7 – – – 12.2 10.8 11.0
Intensity Change (Log2) – – – -0.06 -0.69 -0.62 -0.73 – -0.93 -0.81 -0.92 -0.96 0.1 -0.26 -0.14 – – – 0.27 0.23 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 – 0 -0.1 0 0 0 1.4 0 – – –
Bud Site 0 0 0 0 – 3.9 0 0 5.1 0 0 0 – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cytoplasm 0.4 0.5 -2.2 -1.4 – -0.7 -1.1 0 -0.8 3.8 5.8 7.1 – – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Endosome 0 2.9 3.8 5.5 – 1.5 3.9 3.7 4.1 0 0 0 – – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria 0.7 0 -1.1 -4.1 – 3.9 -2.0 2.7 0.4 4.5 -1.7 -0.9 – – –
Nucleus -4.7 -1.6 -2.5 -2.5 – -2.1 -2.7 -1.9 -1.6 -3.6 -2.7 -3.7 – – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus 3.5 -1.0 -0.2 -0.4 – -0.2 0.1 -0.7 -1.7 1.9 -0.4 -2.4 – – –
Peroxisomes 2.7 1.0 5.2 3.7 – 1.6 3.4 1.2 1.4 0 0.8 0.4 – – –
SpindlePole 2.7 4.6 7.5 9.9 – 6.4 8.8 6.9 9.7 1.8 4.3 2.2 – – –
Vacuole -2.1 -0.4 -2.0 -1.0 – -4.0 -2.0 -2.3 -2.3 0 -2.6 -2.3 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.7178 32.1669 29.3956 27.5117 25.7885 29.7196 23.6403 33.5631 30.0399 26.725 29.7018 29.8867 20.53 26.0795 22.6963 24.4341 22.9869 22.794
Actin 0.0041 0 0.0014 0.0032 0.0024 0 0.0434 0 0 0.0001 0.0003 0.0054 0.0109 0 0.0195 0 0.0614 0.0002
Bud 0.0006 0 0.0001 0.0001 0.0001 0 0.0004 0 0 0.0001 0.0002 0 0.0002 0 0 0 0.0004 0
Bud Neck 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0011 0 0 0.0001 0.0002 0.0009 0.0004 0 0.0001 0 0.0017 0.0001
Bud Periphery 0.0006 0 0.0001 0.0001 0.0001 0.0001 0.0006 0 0 0.0001 0.0006 0.0001 0.0003 0 0 0 0.0006 0.0001
Bud Site 0.0009 0.0001 0.0002 0.0001 0.0002 0.0001 0.0037 0 0.0001 0.0001 0.0002 0.0002 0.0014 0.0001 0.0002 0 0.0043 0.0001
Cell Periphery 0.0006 0.0002 0.0003 0.0005 0.0002 0.0006 0.0011 0.0001 0.0003 0.0004 0.0009 0.0005 0.0006 0.0005 0.0003 0.0003 0.0004 0.0003
Cytoplasm 0.0041 0 0.0002 0.0002 0.0008 0.0003 0.0006 0 0 0.0001 0.0004 0 0.0082 0 0.0021 0 0.0003 0.0055
Cytoplasmic Foci 0.0148 0.0008 0.0086 0.0015 0.0093 0.0037 0.0194 0.0005 0.0087 0.0136 0.024 0.0031 0.0149 0.0001 0.0112 0.0003 0.0043 0.0004
Eisosomes 0.0002 0 0 0 0.0001 0.0001 0.0003 0 0 0.0001 0.0003 0.0001 0.0001 0 0 0 0.0003 0
Endoplasmic Reticulum 0.0017 0 0.0001 0.0022 0.0006 0 0.0008 0 0 0 0.0001 0 0.0015 0 0.0013 0 0.0001 0.0006
Endosome 0.006 0 0.0023 0.0086 0.0054 0.0002 0.0055 0 0 0 0.0002 0.0002 0.0054 0 0.0043 0 0.0019 0.0006
Golgi 0.0017 0 0.0022 0.0057 0.0066 0.0001 0.0093 0 0 0 0.0003 0.0005 0.0024 0 0.0009 0 0.0057 0.0001
Lipid Particles 0.0103 0.0035 0.0046 0.0059 0.0159 0.0088 0.024 0.0026 0.0016 0.0093 0.0325 0.0182 0.0196 0.0005 0.0078 0.0034 0.0036 0.0066
Mitochondria 0.0266 0.0004 0.0081 0.0148 0.0202 0.0452 0.0079 0.0002 0.0032 0.0095 0.0297 0.0103 0.0093 0.0014 0.0022 0.0066 0.0036 0.0246
None 0.0031 0 0.0004 0.0002 0.001 0.0008 0.0013 0 0.0003 0.0008 0.0037 0.0001 0.0012 0 0.001 0 0.0001 0.0002
Nuclear Periphery 0.0291 0.0003 0.003 0.0051 0.0325 0.0061 0.0032 0 0.0002 0.0008 0.0081 0.0122 0.0072 0.0002 0.0008 0.0001 0.0005 0.0151
Nucleolus 0.7935 0.8932 0.8961 0.8731 0.7516 0.8459 0.7853 0.939 0.9457 0.9012 0.7858 0.8668 0.8074 0.9043 0.8987 0.9374 0.7734 0.8595
Nucleus 0.0699 0.1002 0.0627 0.0743 0.1367 0.068 0.0555 0.0572 0.0368 0.0593 0.0935 0.0656 0.0875 0.0884 0.043 0.0495 0.1298 0.0785
Peroxisomes 0.0018 0 0.0014 0.0005 0.0021 0.0003 0.0168 0 0.0003 0.0006 0.0036 0.0004 0.0016 0 0.0022 0.0001 0.005 0.0002
Punctate Nuclear 0.0223 0.0003 0.0025 0.0004 0.0099 0.0014 0.0037 0.0001 0.0007 0.0009 0.002 0.0005 0.0084 0.0005 0.001 0.0002 0.0008 0.0013
Vacuole 0.0027 0.0004 0.0011 0.0009 0.0008 0.0016 0.0025 0.0001 0.0005 0.0009 0.0017 0.0009 0.0022 0.0009 0.0008 0.0005 0.0005 0.0009
Vacuole Periphery 0.0052 0.0007 0.0046 0.0025 0.003 0.0166 0.0135 0.0001 0.0014 0.002 0.0118 0.0139 0.0094 0.003 0.0026 0.0013 0.0012 0.0049

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 153.0407 112.6 103.6071 99.3575 97.9871 55.3915 83.1103 72.6147 53.5473 93.0162
Translational Efficiency 1.3336 1.7768 1.5963 1.3044 1.6598 2.6393 1.7227 1.2333 1.6618 1.5835

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1417 1216 2319 1774 1677 2819 154 1514 3094 4035 2473 3288

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1777.00 2032.37 2086.72 2681.31 1767.39 2184.50 3151.43 3115.58 1771.79 2138.65 2153.02 2881.27
Standard Deviation 447.01 593.47 601.72 853.07 484.16 513.58 675.22 975.07 467.53 543.41 658.87 936.63
Intensity Change Log 2 — 0.193719 0.231793 0.593494 — 0.305683 0.834386 0.817880 — 0.250635 0.563520 0.709742

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000681 0.000664 0.000958 0.001153 0.000631 0.000663 0.000664 0.001334 0.000654 0.000663 0.000940 0.001236
Bud Neck 0.000762 0.001535 0.004833 0.011060 0.000753 0.001240 0.002060 0.006991 0.000757 0.001329 0.004660 0.009187
Bud Site 0.000231 0.000712 0.000750 0.001770 0.000123 0.000211 0.000335 0.001600 0.000172 0.000362 0.000724 0.001691
Cell Periphery 0.000022 0.000030 0.000069 0.000190 0.000017 0.000041 0.000018 0.000183 0.000020 0.000038 0.000066 0.000187
Cytoplasm 0.000095 0.000250 0.000439 0.000911 0.000157 0.000131 0.000021 0.001076 0.000129 0.000167 0.000413 0.000987
Cytoplasmic Foci 0.001202 0.006896 0.001991 0.005387 0.002777 0.002711 0.003052 0.005215 0.002056 0.003972 0.002057 0.005308
Eisosomes 0.000217 0.000313 0.000146 0.000150 0.000372 0.000397 0.000165 0.000292 0.000301 0.000371 0.000147 0.000215
Endoplasmic Reticulum 0.000203 0.000133 0.000290 0.000240 0.000129 0.000162 0.000059 0.000191 0.000163 0.000153 0.000275 0.000217
Endosome 0.000022 0.000052 0.000081 0.001093 0.000017 0.000033 0.000016 0.000314 0.000019 0.000039 0.000077 0.000735
Golgi 0.000100 0.000123 0.000170 0.000784 0.000081 0.000090 0.000218 0.000606 0.000090 0.000100 0.000173 0.000702
Lipid Particles 0.000056 0.000060 0.000029 0.000103 0.000057 0.000040 0.000024 0.000166 0.000056 0.000046 0.000029 0.000132
Mitochondria 0.001597 0.002313 0.003489 0.006714 0.001782 0.002093 0.002880 0.008007 0.001697 0.002159 0.003451 0.007309
Mitotic Spindle 0.000831 0.000473 0.005208 0.007308 0.000680 0.001083 0.008047 0.004073 0.000749 0.000899 0.005385 0.005818
None 0.000614 0.000759 0.001124 0.001579 0.000452 0.000557 0.000400 0.001335 0.000526 0.000618 0.001079 0.001467
Nuclear Periphery 0.001350 0.001030 0.003574 0.004780 0.002184 0.001891 0.005819 0.015202 0.001802 0.001632 0.003714 0.009579
Nuclear Periphery Foci 0.000697 0.000768 0.001380 0.001796 0.001640 0.001145 0.001582 0.005261 0.001208 0.001031 0.001392 0.003391
Nucleolus 0.716534* 0.692595* 0.585287* 0.528688* 0.748164* 0.690751* 0.786684* 0.638201* 0.733678* 0.691306* 0.597829* 0.579114*
Nucleus 0.244740* 0.222515* 0.360419* 0.391449* 0.179383* 0.251126* 0.099476 0.247664* 0.209315* 0.242504* 0.344170* 0.325242*
Peroxisomes 0.027853 0.063966 0.025391 0.021816 0.057333 0.042197 0.085214 0.021524 0.043831 0.048758 0.029116 0.021682
Vacuole 0.000521 0.001472 0.000788 0.003334 0.000717 0.000814 0.000637 0.005676 0.000627 0.001013 0.000779 0.004413
Vacuole Periphery 0.001672 0.003341 0.003583 0.009695 0.002552 0.002623 0.002631 0.035087 0.002149 0.002840 0.003524 0.021387

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.11 -4.83 -7.88 -9.08 -4.50 -1.10 -0.45 -13.78 -13.33 -8.53 -0.42 -7.69 -15.31 -16.83 -7.66
Bud Neck -4.60 -13.05 -16.52 -15.16 -9.58 -5.80 -4.21 -13.87 -12.82 -9.11 -7.26 -13.62 -21.18 -19.79 -9.85
Bud Site -1.54 -1.91 -7.17 -3.74 -4.85 -2.13 -5.36 -3.63 -3.34 -3.13 -1.72 -2.87 -6.97 -6.01 -3.90
Cell Periphery -1.30 -4.18 -8.15 -7.49 -5.55 -2.64 -0.29 -3.03 -2.46 -3.01 -2.76 -4.70 -6.23 -5.25 -4.32
Cytoplasm -1.49 -1.93 -7.46 -4.25 -2.81 -0.11 1.08 -2.33 -2.34 -2.61 -0.79 -1.59 -3.81 -3.58 -2.67
Cytoplasmic Foci -4.57 -1.93 -6.31 0.66 -5.11 -0.02 -0.50 -3.51 -3.59 -3.01 -3.64 -0.59 -6.79 -2.76 -6.13
Eisosomes -2.42 3.91 3.57 4.47 -0.54 -1.00 9.58 2.11 2.72 -3.27 -3.44 11.22 4.16 6.69 -3.75
Endoplasmic Reticulum 1.28 -1.60 -0.84 -3.66 1.24 -1.62 5.67 -3.50 -1.28 -8.19 0.34 -3.34 -2.21 -3.46 1.91
Endosome -1.63 -2.58 -2.93 -2.84 -2.75 -2.23 0.18 -1.95 -1.81 -1.95 -2.47 -2.89 -3.42 -3.31 -3.10
Golgi -0.63 -2.09 -2.59 -2.52 -2.33 -0.95 -4.10 -2.37 -2.31 -1.67 -0.66 -5.11 -3.50 -3.44 -3.01
Lipid Particles -0.20 0.77 -1.22 -1.09 -2.62 0.73 1.59 -2.00 -2.36 -2.68 0.45 1.08 -2.39 -2.93 -3.30
Mitochondria -2.31 -5.77 -10.42 -9.29 -6.67 -1.69 -2.72 -8.46 -7.97 -6.29 -2.69 -7.04 -13.22 -12.19 -8.39
Mitotic Spindle 0.84 -4.25 -5.09 -6.03 -1.49 -1.30 -2.01 -4.33 -3.63 1.40 -0.27 -5.36 -6.67 -6.72 -0.15
None -1.66 -2.53 -7.12 -6.31 -2.16 -1.61 0.50 -5.70 -4.67 -5.55 -1.65 -3.08 -9.37 -8.00 -2.02
Nuclear Periphery 0.79 -4.45 -4.82 -5.38 -1.52 0.53 -1.29 -7.73 -8.03 -2.93 0.46 -3.85 -8.88 -9.18 -6.30
Nuclear Periphery Foci -0.22 -3.10 -3.12 -2.72 -1.20 1.33 0.09 -6.10 -8.16 -3.91 0.77 -0.78 -6.31 -7.68 -6.22
Nucleolus 2.57 14.24 19.09 16.63 6.32 8.68 -2.06 12.75 6.45 9.14 7.70 18.64 23.48 17.72 2.82
Nucleus 2.32 -12.31 -14.73 -16.91 -3.50 -10.92 6.39 -8.21 0.13 -11.15 -6.00 -17.96 -17.53 -12.98 1.85
Peroxisomes -13.29 1.27 3.28 15.19 2.25 6.52 -3.48 15.83 12.27 7.88 -2.95 8.65 14.81 18.81 5.43
Vacuole -3.38 -1.90 -6.99 -3.99 -6.10 -1.14 0.68 -10.09 -9.70 -10.11 -3.54 -1.34 -11.96 -10.21 -10.69
Vacuole Periphery -3.09 -5.78 -9.62 -6.49 -7.09 -0.19 -0.07 -13.45 -13.48 -13.32 -2.41 -4.25 -15.71 -15.07 -14.44
Standard name
Human Ortholog
Description Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
Localization
Cell Percentages nucleolus (61%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Brx1

Brx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available