Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 0 |
Bud | 2 | 2 | 2 | 3 | 1 | 2 | – | 10 | 10 | 7 | 15 | 9 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 1 | 2 | 9 | 4 | 5 | – | 7 | 6 | 8 | 9 | 11 | 8 | 19 | 41 | 16 | 32 | 29 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 346 | 52 | 117 | 283 | 392 | 381 | – | 532 | 358 | 331 | 338 | 346 | 390 | 457 | 516 | 169 | 161 | 199 | 343 | 71 | 117 | 107 | 315 | 324 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 0 | 3 | – | 2 | 2 | 3 | 1 | 7 | 1 | 0 | 3 | 8 | 11 | 12 | 0 | 0 | 1 | 1 | 5 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 6 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 7 | 1 | 5 | 28 | 31 | 107 | – | 234 | 272 | 217 | 253 | 348 | 2 | 2 | 0 | 5 | 7 | 4 | 1 | 0 | 3 | 1 | 5 | 8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 1 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | – | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 2 | – | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 2 | 1 | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 1 | 2 | – | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 2 | 6 | 15 | 6 | 0 | 0 | 5 | 2 | 8 | 6 |
Unique Cell Count | 352 | 55 | 121 | 290 | 413 | 414 | 663 | 496 | 470 | 471 | 578 | 392 | 461 | 520 | 183 | 183 | 211 | 358 | 73 | 135 | 116 | 352 | 367 | |
Labelled Cell Count | 366 | 58 | 127 | 325 | 435 | 503 | 799 | 656 | 579 | 619 | 739 | 403 | 483 | 563 | 211 | 234 | 257 | 358 | 73 | 135 | 116 | 352 | 367 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.9 | 3.8 | 4.3 | 4.9 | 3.8 | – | 3.8 | 3.1 | 3.5 | 3.1 | 3.2 | 5.7 | 5.9 | 5.8 | 7.3 | 7.3 | 7.0 | 5.6 | 6.3 | 6.4 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.9 | 1.1 | 1.8 | 0.9 | – | 1.0 | 0.5 | 0.7 | 0.7 | 0.6 | 0.7 | 0.9 | 1.2 | 1.2 | 1.4 | 1.3 | 0.6 | 1.4 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.34 | -0.03 | – | -0.01 | -0.33 | -0.13 | -0.29 | -0.27 | 0.57 | 0.61 | 0.6 | 0.91 | 0.92 | 0.85 | 0.53 | 0.7 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.6 | 4.3 | 3.6 |
Cytoplasm | 0.5 | -0.8 | -1.8 | – | -4.4 | -5.7 | -6.0 | -5.8 | -7.8 | 2.5 | 2.1 | 2.3 | -1.6 | -2.7 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0829 | 2.8243 | 2.3646 | 2.1994 | 2.0209 | 2.6099 | 0.6428 | 1.4835 | 1.2052 | 0.8181 | 1.0824 | 1.2681 | 1.7987 | 3.1056 | 2.4014 | 2.9745 | 2.4788 | 2.5262 |
Actin | 0.0214 | 0.0015 | 0.0061 | 0.0012 | 0.0088 | 0.0076 | 0.0038 | 0.0012 | 0.0014 | 0.0121 | 0.0054 | 0.0014 | 0.0156 | 0.0003 | 0.0201 | 0.0162 | 0.0013 | 0.0013 |
Bud | 0.0005 | 0.0026 | 0.0018 | 0.0043 | 0.0118 | 0.0024 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0007 | 0.0018 | 0.0007 | 0.0002 | 0.0022 | 0.0019 | 0.0004 | 0.0001 |
Bud Neck | 0.0029 | 0.0006 | 0.0029 | 0.0011 | 0.0041 | 0.0213 | 0.0008 | 0.0002 | 0.0006 | 0.0008 | 0.0011 | 0.0053 | 0.0067 | 0.0001 | 0.0015 | 0.0118 | 0.0002 | 0.0004 |
Bud Periphery | 0.0007 | 0.0015 | 0.0049 | 0.0085 | 0.0226 | 0.0037 | 0.0004 | 0.0001 | 0.0003 | 0.001 | 0.0012 | 0.0027 | 0.0011 | 0.0002 | 0.0041 | 0.003 | 0.0007 | 0.0001 |
Bud Site | 0.0074 | 0.0253 | 0.0074 | 0.0015 | 0.0105 | 0.0036 | 0.0055 | 0.0015 | 0.0013 | 0.0068 | 0.0011 | 0.0046 | 0.0107 | 0.0021 | 0.0046 | 0.0261 | 0.0003 | 0.0001 |
Cell Periphery | 0.0026 | 0.0015 | 0.002 | 0.0009 | 0.0014 | 0.0006 | 0.0008 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0016 | 0.0005 | 0.0008 | 0.001 | 0.0002 | 0.0002 |
Cytoplasm | 0.7024 | 0.7476 | 0.6875 | 0.6399 | 0.7465 | 0.7403 | 0.5967 | 0.8242 | 0.7564 | 0.6078 | 0.6605 | 0.877 | 0.5227 | 0.7863 | 0.6625 | 0.5782 | 0.8228 | 0.7781 |
Cytoplasmic Foci | 0.02 | 0.0088 | 0.0244 | 0.0309 | 0.0092 | 0.007 | 0.0441 | 0.0074 | 0.0063 | 0.0489 | 0.0434 | 0.0078 | 0.0129 | 0.0026 | 0.0053 | 0.0122 | 0.0063 | 0.0028 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0035 | 0.0027 | 0.0038 | 0.0019 | 0.0036 | 0.0031 | 0.0078 | 0.0028 | 0.0035 | 0.0067 | 0.0046 | 0.0018 | 0.0047 | 0.0016 | 0.0023 | 0.0076 | 0.0029 | 0.0011 |
Endosome | 0.0094 | 0.0019 | 0.0057 | 0.011 | 0.008 | 0.0066 | 0.0494 | 0.0014 | 0.0094 | 0.0728 | 0.0605 | 0.0051 | 0.0202 | 0.0008 | 0.0052 | 0.0306 | 0.0166 | 0.001 |
Golgi | 0.0025 | 0.0004 | 0.0013 | 0.0017 | 0.0006 | 0.0023 | 0.0095 | 0.0004 | 0.002 | 0.0203 | 0.0325 | 0.001 | 0.005 | 0.0001 | 0.0065 | 0.0095 | 0.0062 | 0.0004 |
Lipid Particles | 0.0045 | 0.0003 | 0.0016 | 0.0116 | 0.0003 | 0.0027 | 0.0153 | 0.0005 | 0.0038 | 0.0104 | 0.0125 | 0.0005 | 0.0088 | 0.0001 | 0.0009 | 0.0021 | 0.0011 | 0.0001 |
Mitochondria | 0.0007 | 0.0002 | 0.0004 | 0.0011 | 0.0006 | 0.0043 | 0.0069 | 0.0001 | 0.004 | 0.0173 | 0.0289 | 0.0007 | 0.0017 | 0.0001 | 0.0029 | 0.0093 | 0.035 | 0.0001 |
None | 0.2052 | 0.1996 | 0.2415 | 0.2595 | 0.159 | 0.1856 | 0.2092 | 0.1569 | 0.2039 | 0.1675 | 0.1258 | 0.072 | 0.3611 | 0.2036 | 0.2763 | 0.2692 | 0.1012 | 0.2134 |
Nuclear Periphery | 0.0016 | 0.0004 | 0.0007 | 0.0004 | 0.0014 | 0.0028 | 0.0085 | 0.0002 | 0.0004 | 0.003 | 0.0013 | 0.0005 | 0.0027 | 0.0001 | 0.0004 | 0.0069 | 0.0008 | 0.0001 |
Nucleolus | 0.0004 | 0.0002 | 0.0003 | 0.0016 | 0.0009 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0024 | 0.0046 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 |
Nucleus | 0.0014 | 0.0011 | 0.0012 | 0.0013 | 0.0035 | 0.0018 | 0.0173 | 0.0005 | 0.0005 | 0.0014 | 0.0007 | 0.0055 | 0.0049 | 0.0003 | 0.0006 | 0.0032 | 0.0006 | 0.0002 |
Peroxisomes | 0.0045 | 0.0002 | 0.0014 | 0.0129 | 0.0003 | 0.0008 | 0.0051 | 0.0002 | 0.001 | 0.0093 | 0.0075 | 0.0003 | 0.0041 | 0 | 0.001 | 0.0013 | 0.0004 | 0 |
Punctate Nuclear | 0.0055 | 0.0014 | 0.0024 | 0.0018 | 0.0013 | 0.0008 | 0.0078 | 0.0003 | 0.0002 | 0.0044 | 0.0004 | 0.0007 | 0.0026 | 0.0001 | 0.0002 | 0.0016 | 0.0003 | 0.0001 |
Vacuole | 0.0025 | 0.0019 | 0.0025 | 0.0062 | 0.0049 | 0.002 | 0.0078 | 0.0011 | 0.0035 | 0.0061 | 0.0071 | 0.0078 | 0.0065 | 0.0009 | 0.0018 | 0.0054 | 0.0018 | 0.0005 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0006 | 0.0004 | 0.0017 | 0.0001 | 0.0004 | 0.0018 | 0.0042 | 0.0008 | 0.0007 | 0.0001 | 0.0002 | 0.0022 | 0.0008 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.0181 | 29.6767 | 24.0809 | 35.4415 | 24.3854 | 26.7344 | 36.1314 | 38.2458 | 31.315 | 36.2641 |
Translational Efficiency | 0.1723 | 0.1551 | 0.1876 | 0.1129 | 0.1257 | 0.1727 | 0.1563 | 0.0958 | 0.135 | 0.1099 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
36 | 1417 | 448 | 159 | 1180 | 1374 | 1317 | 51 | 1216 | 2791 | 1765 | 210 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 850.90 | 789.39 | 965.91 | 974.34 | 691.96 | 783.33 | 776.02 | 833.15 | 696.67 | 786.41 | 824.22 | 940.05 |
Standard Deviation | 120.06 | 114.09 | 108.11 | 101.26 | 91.12 | 99.40 | 92.21 | 79.88 | 95.96 | 107.15 | 127.05 | 113.93 |
Intensity Change Log 2 | — | -0.108251 | 0.182899 | 0.195436 | — | 0.178932 | 0.165405 | 0.267888 | — | 0.027655 | 0.175079 | 0.228381 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000646 | 0.000319 | 0.000261 | 0.000291 | 0.000367 | 0.000324 | 0.000296 | 0.000213 | 0.000375 | 0.000322 | 0.000288 | 0.000272 |
Bud Neck | 0.002583 | 0.013866 | 0.002243 | 0.003543 | 0.022208 | 0.032034 | 0.006150 | 0.003414 | 0.021627 | 0.022810 | 0.005158 | 0.003511 |
Bud Site | 0.004629 | 0.023830 | 0.017147 | 0.052381 | 0.010946 | 0.024164 | 0.034428 | 0.052057 | 0.010759 | 0.023994 | 0.030042 | 0.052302 |
Cell Periphery | 0.000689 | 0.000151 | 0.000240 | 0.000130 | 0.000296 | 0.000174 | 0.000150 | 0.000864 | 0.000308 | 0.000162 | 0.000173 | 0.000308 |
Cytoplasm | 0.478631* | 0.589360* | 0.598822* | 0.464220* | 0.498218* | 0.507579* | 0.686619* | 0.503101* | 0.497638* | 0.549100* | 0.664334* | 0.473662* |
Cytoplasmic Foci | 0.175570 | 0.203605* | 0.131543 | 0.080465 | 0.283700* | 0.244915* | 0.089533 | 0.102847 | 0.280499* | 0.223942* | 0.100196 | 0.085900 |
Eisosomes | 0.000390 | 0.000125 | 0.000075 | 0.000054 | 0.000608 | 0.000224 | 0.000064 | 0.000131 | 0.000602 | 0.000174 | 0.000066 | 0.000073 |
Endoplasmic Reticulum | 0.008129 | 0.000663 | 0.001058 | 0.000517 | 0.001483 | 0.000700 | 0.000309 | 0.000613 | 0.001680 | 0.000681 | 0.000499 | 0.000540 |
Endosome | 0.007146 | 0.025060 | 0.013878 | 0.009414 | 0.029983 | 0.037498 | 0.006907 | 0.016896 | 0.029307 | 0.031183 | 0.008677 | 0.011231 |
Golgi | 0.000542 | 0.008043 | 0.000335 | 0.000176 | 0.009431 | 0.008055 | 0.001062 | 0.016437 | 0.009168 | 0.008049 | 0.000878 | 0.004125 |
Lipid Particles | 0.009986 | 0.003077 | 0.002108 | 0.001084 | 0.011407 | 0.004155 | 0.000868 | 0.001935 | 0.011365 | 0.003608 | 0.001183 | 0.001291 |
Mitochondria | 0.001264 | 0.003672 | 0.000062 | 0.000535 | 0.002654 | 0.004188 | 0.000811 | 0.004295 | 0.002613 | 0.003926 | 0.000621 | 0.001448 |
Mitotic Spindle | 0.028576 | 0.003375 | 0.001004 | 0.007776 | 0.000304 | 0.001983 | 0.004864 | 0.000164 | 0.001141 | 0.002690 | 0.003884 | 0.005928 |
None | 0.000378 | 0.001609 | 0.001105 | 0.000679 | 0.000825 | 0.000978 | 0.000564 | 0.000818 | 0.000812 | 0.001298 | 0.000702 | 0.000713 |
Nuclear Periphery | 0.008562 | 0.000197 | 0.000241 | 0.000284 | 0.000234 | 0.000097 | 0.000072 | 0.000391 | 0.000480 | 0.000148 | 0.000115 | 0.000310 |
Nuclear Periphery Foci | 0.013098 | 0.000836 | 0.005050 | 0.002077 | 0.000486 | 0.000239 | 0.000683 | 0.001467 | 0.000859 | 0.000542 | 0.001792 | 0.001929 |
Nucleolus | 0.001068 | 0.000387 | 0.000192 | 0.000152 | 0.000377 | 0.000295 | 0.000104 | 0.000327 | 0.000397 | 0.000342 | 0.000126 | 0.000194 |
Nucleus | 0.105114 | 0.020442 | 0.032944 | 0.013171 | 0.034887 | 0.022686 | 0.023184 | 0.068652 | 0.036966 | 0.021547 | 0.025661 | 0.026645 |
Peroxisomes | 0.001353 | 0.004312 | 0.001531 | 0.000953 | 0.004024 | 0.005065 | 0.001217 | 0.000468 | 0.003945 | 0.004683 | 0.001297 | 0.000835 |
Vacuole | 0.151072 | 0.095412 | 0.189892 | 0.361356* | 0.085668 | 0.102292 | 0.141268 | 0.223750* | 0.087605 | 0.098799 | 0.153610 | 0.327937* |
Vacuole Periphery | 0.000574 | 0.001658 | 0.000268 | 0.000743 | 0.001892 | 0.002353 | 0.000848 | 0.001160 | 0.001853 | 0.002000 | 0.000701 | 0.000844 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.10 | 1.32 | 1.56 | 3.24 | 1.12 | 0.48 | 1.08 | 1.47 | 1.01 | 0.74 | 0.78 | 1.39 | 2.69 | 2.63 | 1.70 |
Bud Neck | -9.83 | 0.22 | 0.51 | 11.00 | 0.53 | -3.82 | 9.71 | 10.77 | 12.88 | 2.29 | -0.61 | 10.59 | 12.64 | 17.01 | 3.70 |
Bud Site | -9.01 | -3.41 | -2.21 | 0.62 | -0.51 | -7.87 | -6.81 | -1.11 | -0.53 | -0.23 | -10.00 | -6.88 | -1.70 | 0.06 | 0.46 |
Cell Periphery | 4.13 | 3.52 | 4.77 | 5.90 | 7.17 | 8.15 | 9.28 | -0.89 | -1.20 | -1.29 | 9.92 | 9.06 | 0.95 | -0.52 | -0.55 |
Cytoplasm | -2.62 | -2.29 | 1.44 | 8.79 | 7.54 | -0.93 | -17.61 | 0.82 | 1.07 | 5.54 | -6.09 | -16.15 | 4.83 | 7.43 | 11.93 |
Cytoplasmic Foci | -1.20 | 1.57 | 3.72 | 18.75 | 7.71 | 5.28 | 32.48 | 14.52 | 11.71 | -0.14 | 8.93 | 31.45 | 28.38 | 24.25 | 4.82 |
Eisosomes | 3.76 | 4.54 | 4.95 | 9.99 | 4.66 | 8.01 | 12.36 | 9.91 | 3.49 | -3.72 | 9.71 | 12.49 | 12.31 | 9.29 | -0.58 |
Endoplasmic Reticulum | 1.31 | 1.22 | 1.35 | 0.96 | 5.45 | 3.29 | 5.77 | 1.32 | -0.66 | -1.80 | 3.56 | 4.63 | 4.10 | 0.67 | -0.66 |
Endosome | -7.75 | -2.82 | 1.39 | 10.26 | 5.20 | -3.04 | 13.86 | 3.54 | 5.20 | -2.17 | -0.91 | 12.87 | 10.74 | 13.36 | 0.59 |
Golgi | -8.65 | 1.35 | 2.92 | 9.16 | 3.76 | 1.34 | 12.27 | -0.14 | -0.27 | -0.93 | 1.36 | 12.79 | 2.37 | 1.95 | -0.75 |
Lipid Particles | 2.24 | 2.62 | 3.00 | 6.07 | 4.81 | 7.45 | 11.25 | 7.16 | 2.05 | -1.75 | 8.31 | 11.20 | 10.84 | 7.51 | -0.57 |
Mitochondria | -2.08 | 1.23 | 1.04 | 3.67 | -0.82 | -1.33 | 2.78 | -0.09 | 0.46 | -0.82 | -1.40 | 3.09 | 1.90 | 3.28 | -0.46 |
Mitotic Spindle | 0.87 | 0.97 | 0.78 | -0.77 | -1.76 | -2.02 | -3.26 | 0.40 | 2.11 | 3.32 | -1.78 | -2.21 | -1.55 | -0.72 | -0.19 |
None | -3.82 | -3.20 | -2.14 | 2.23 | 0.94 | -0.36 | 2.21 | -0.33 | 0.06 | -1.17 | -1.75 | 1.16 | 0.01 | 1.60 | -0.81 |
Nuclear Periphery | 1.03 | 1.02 | 1.00 | -2.39 | -1.95 | 3.31 | 3.50 | -2.37 | -3.44 | -3.48 | 1.41 | 1.47 | 0.08 | -4.84 | -5.82 |
Nuclear Periphery Foci | 1.23 | 0.76 | 1.12 | -3.31 | 6.65 | 3.46 | -2.50 | -1.56 | -1.75 | -1.41 | 0.94 | -3.25 | -2.65 | -3.93 | -0.62 |
Nucleolus | 0.79 | 1.13 | 1.24 | 5.21 | 2.33 | 1.73 | 6.60 | -0.11 | -0.65 | -1.91 | 0.90 | 6.07 | 3.49 | 3.32 | -1.23 |
Nucleus | 3.37 | 2.74 | 3.55 | 2.33 | 6.32 | 6.25 | 4.51 | -3.83 | -4.83 | -4.57 | 8.20 | 4.17 | 1.14 | -2.75 | -0.97 |
Peroxisomes | -6.98 | -0.14 | 2.40 | 10.99 | 1.30 | -1.83 | 6.24 | 10.36 | 8.73 | 2.54 | -1.77 | 6.41 | 10.86 | 13.36 | 2.80 |
Vacuole | 2.39 | -0.63 | -6.58 | -16.77 | -10.83 | -3.70 | -12.94 | -6.06 | -5.54 | -3.65 | -2.72 | -15.66 | -17.06 | -16.66 | -12.78 |
Vacuole Periphery | -3.59 | 0.98 | 0.65 | 4.94 | -0.19 | -2.31 | 3.48 | 2.08 | 3.01 | -0.17 | -0.76 | 4.69 | 5.94 | 6.99 | 0.66 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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