Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 0 |
Bud | 2 | 2 | 2 | 3 | 1 | 2 | – | 10 | 10 | 7 | 15 | 9 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | – | 3 | 0 | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 1 | 2 | 9 | 4 | 5 | – | 7 | 6 | 8 | 9 | 11 | 8 | 19 | 41 | 16 | 32 | 29 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 346 | 52 | 117 | 283 | 392 | 381 | – | 532 | 358 | 331 | 338 | 346 | 390 | 457 | 516 | 169 | 161 | 199 | 343 | 71 | 117 | 107 | 315 | 324 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 0 | 3 | – | 2 | 2 | 3 | 1 | 7 | 1 | 0 | 3 | 8 | 11 | 12 | 0 | 0 | 1 | 1 | 5 | 3 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 6 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 7 | 1 | 5 | 28 | 31 | 107 | – | 234 | 272 | 217 | 253 | 348 | 2 | 2 | 0 | 5 | 7 | 4 | 1 | 0 | 3 | 1 | 5 | 8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 1 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | – | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 2 | – | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 2 | 1 | 0 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 1 | 2 | – | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 2 | 6 | 15 | 6 | 0 | 0 | 5 | 2 | 8 | 6 |
Unique Cell Count | 352 | 55 | 121 | 290 | 413 | 414 | 663 | 496 | 470 | 471 | 578 | 392 | 461 | 520 | 183 | 183 | 211 | 358 | 73 | 135 | 116 | 352 | 367 | |
Labelled Cell Count | 366 | 58 | 127 | 325 | 435 | 503 | 799 | 656 | 579 | 619 | 739 | 403 | 483 | 563 | 211 | 234 | 257 | 358 | 73 | 135 | 116 | 352 | 367 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.9 | 3.8 | 4.3 | 4.9 | 3.8 | – | 3.8 | 3.1 | 3.5 | 3.1 | 3.2 | 5.7 | 5.9 | 5.8 | 7.3 | 7.3 | 7.0 | 5.6 | 6.3 | 6.4 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.9 | 1.1 | 1.8 | 0.9 | – | 1.0 | 0.5 | 0.7 | 0.7 | 0.6 | 0.7 | 0.9 | 1.2 | 1.2 | 1.4 | 1.3 | 0.6 | 1.4 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.34 | -0.03 | – | -0.01 | -0.33 | -0.13 | -0.29 | -0.27 | 0.57 | 0.61 | 0.6 | 0.91 | 0.92 | 0.85 | 0.53 | 0.7 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.6 | 4.3 | 3.6 |
Cytoplasm | 0.5 | -0.8 | -1.8 | – | -4.4 | -5.7 | -6.0 | -5.8 | -7.8 | 2.5 | 2.1 | 2.3 | -1.6 | -2.7 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0829 | 2.8243 | 2.3646 | 2.1994 | 2.0209 | 2.6099 | 0.6428 | 1.4835 | 1.2052 | 0.8181 | 1.0824 | 1.2681 | 1.7987 | 3.1056 | 2.4014 | 2.9745 | 2.4788 | 2.5262 |
Actin | 0.0214 | 0.0015 | 0.0061 | 0.0012 | 0.0088 | 0.0076 | 0.0038 | 0.0012 | 0.0014 | 0.0121 | 0.0054 | 0.0014 | 0.0156 | 0.0003 | 0.0201 | 0.0162 | 0.0013 | 0.0013 |
Bud | 0.0005 | 0.0026 | 0.0018 | 0.0043 | 0.0118 | 0.0024 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0007 | 0.0018 | 0.0007 | 0.0002 | 0.0022 | 0.0019 | 0.0004 | 0.0001 |
Bud Neck | 0.0029 | 0.0006 | 0.0029 | 0.0011 | 0.0041 | 0.0213 | 0.0008 | 0.0002 | 0.0006 | 0.0008 | 0.0011 | 0.0053 | 0.0067 | 0.0001 | 0.0015 | 0.0118 | 0.0002 | 0.0004 |
Bud Periphery | 0.0007 | 0.0015 | 0.0049 | 0.0085 | 0.0226 | 0.0037 | 0.0004 | 0.0001 | 0.0003 | 0.001 | 0.0012 | 0.0027 | 0.0011 | 0.0002 | 0.0041 | 0.003 | 0.0007 | 0.0001 |
Bud Site | 0.0074 | 0.0253 | 0.0074 | 0.0015 | 0.0105 | 0.0036 | 0.0055 | 0.0015 | 0.0013 | 0.0068 | 0.0011 | 0.0046 | 0.0107 | 0.0021 | 0.0046 | 0.0261 | 0.0003 | 0.0001 |
Cell Periphery | 0.0026 | 0.0015 | 0.002 | 0.0009 | 0.0014 | 0.0006 | 0.0008 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0016 | 0.0005 | 0.0008 | 0.001 | 0.0002 | 0.0002 |
Cytoplasm | 0.7024 | 0.7476 | 0.6875 | 0.6399 | 0.7465 | 0.7403 | 0.5967 | 0.8242 | 0.7564 | 0.6078 | 0.6605 | 0.877 | 0.5227 | 0.7863 | 0.6625 | 0.5782 | 0.8228 | 0.7781 |
Cytoplasmic Foci | 0.02 | 0.0088 | 0.0244 | 0.0309 | 0.0092 | 0.007 | 0.0441 | 0.0074 | 0.0063 | 0.0489 | 0.0434 | 0.0078 | 0.0129 | 0.0026 | 0.0053 | 0.0122 | 0.0063 | 0.0028 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0035 | 0.0027 | 0.0038 | 0.0019 | 0.0036 | 0.0031 | 0.0078 | 0.0028 | 0.0035 | 0.0067 | 0.0046 | 0.0018 | 0.0047 | 0.0016 | 0.0023 | 0.0076 | 0.0029 | 0.0011 |
Endosome | 0.0094 | 0.0019 | 0.0057 | 0.011 | 0.008 | 0.0066 | 0.0494 | 0.0014 | 0.0094 | 0.0728 | 0.0605 | 0.0051 | 0.0202 | 0.0008 | 0.0052 | 0.0306 | 0.0166 | 0.001 |
Golgi | 0.0025 | 0.0004 | 0.0013 | 0.0017 | 0.0006 | 0.0023 | 0.0095 | 0.0004 | 0.002 | 0.0203 | 0.0325 | 0.001 | 0.005 | 0.0001 | 0.0065 | 0.0095 | 0.0062 | 0.0004 |
Lipid Particles | 0.0045 | 0.0003 | 0.0016 | 0.0116 | 0.0003 | 0.0027 | 0.0153 | 0.0005 | 0.0038 | 0.0104 | 0.0125 | 0.0005 | 0.0088 | 0.0001 | 0.0009 | 0.0021 | 0.0011 | 0.0001 |
Mitochondria | 0.0007 | 0.0002 | 0.0004 | 0.0011 | 0.0006 | 0.0043 | 0.0069 | 0.0001 | 0.004 | 0.0173 | 0.0289 | 0.0007 | 0.0017 | 0.0001 | 0.0029 | 0.0093 | 0.035 | 0.0001 |
None | 0.2052 | 0.1996 | 0.2415 | 0.2595 | 0.159 | 0.1856 | 0.2092 | 0.1569 | 0.2039 | 0.1675 | 0.1258 | 0.072 | 0.3611 | 0.2036 | 0.2763 | 0.2692 | 0.1012 | 0.2134 |
Nuclear Periphery | 0.0016 | 0.0004 | 0.0007 | 0.0004 | 0.0014 | 0.0028 | 0.0085 | 0.0002 | 0.0004 | 0.003 | 0.0013 | 0.0005 | 0.0027 | 0.0001 | 0.0004 | 0.0069 | 0.0008 | 0.0001 |
Nucleolus | 0.0004 | 0.0002 | 0.0003 | 0.0016 | 0.0009 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0024 | 0.0046 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 |
Nucleus | 0.0014 | 0.0011 | 0.0012 | 0.0013 | 0.0035 | 0.0018 | 0.0173 | 0.0005 | 0.0005 | 0.0014 | 0.0007 | 0.0055 | 0.0049 | 0.0003 | 0.0006 | 0.0032 | 0.0006 | 0.0002 |
Peroxisomes | 0.0045 | 0.0002 | 0.0014 | 0.0129 | 0.0003 | 0.0008 | 0.0051 | 0.0002 | 0.001 | 0.0093 | 0.0075 | 0.0003 | 0.0041 | 0 | 0.001 | 0.0013 | 0.0004 | 0 |
Punctate Nuclear | 0.0055 | 0.0014 | 0.0024 | 0.0018 | 0.0013 | 0.0008 | 0.0078 | 0.0003 | 0.0002 | 0.0044 | 0.0004 | 0.0007 | 0.0026 | 0.0001 | 0.0002 | 0.0016 | 0.0003 | 0.0001 |
Vacuole | 0.0025 | 0.0019 | 0.0025 | 0.0062 | 0.0049 | 0.002 | 0.0078 | 0.0011 | 0.0035 | 0.0061 | 0.0071 | 0.0078 | 0.0065 | 0.0009 | 0.0018 | 0.0054 | 0.0018 | 0.0005 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0006 | 0.0004 | 0.0017 | 0.0001 | 0.0004 | 0.0018 | 0.0042 | 0.0008 | 0.0007 | 0.0001 | 0.0002 | 0.0022 | 0.0008 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.0181 | 29.6767 | 24.0809 | 35.4415 | 24.3854 | 26.7344 | 36.1314 | 38.2458 | 31.315 | 36.2641 |
Translational Efficiency | 0.1723 | 0.1551 | 0.1876 | 0.1129 | 0.1257 | 0.1727 | 0.1563 | 0.0958 | 0.135 | 0.1099 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|