Standard name | |
---|---|
Human Ortholog | |
Description | Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 23 | 63 | 69 | 26 | 37 | 32 | 33 | 39 | 36 | 57 | 55 | 39 | 37 | 39 | 34 | – | 19 | 8 | 29 | 35 | 39 | 24 | 25 | 22 |
Bud | 4 | 21 | 17 | 15 | 9 | 9 | 14 | 30 | 20 | 28 | 19 | 23 | 13 | 13 | 9 | – | 2 | 3 | 3 | 2 | 0 | 0 | 3 | 7 |
Bud Neck | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 8 | 1 | – | 3 | 6 | 14 | 29 | 12 | 13 | 18 | 19 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 77 | 201 | 158 | 92 | 103 | 82 | 113 | 92 | 114 | 163 | 110 | 190 | 413 | 413 | 292 | – | 90 | 57 | 154 | 307 | 288 | 175 | 235 | 241 |
Cytoplasm | 144 | 205 | 147 | 107 | 123 | 110 | 160 | 127 | 133 | 169 | 117 | 164 | 470 | 470 | 542 | – | 166 | 104 | 25 | 29 | 6 | 23 | 18 | 27 |
Endoplasmic Reticulum | 2 | 4 | 2 | 4 | 3 | 5 | 7 | 5 | 3 | 6 | 14 | 11 | 11 | 7 | 5 | – | 18 | 13 | 13 | 11 | 12 | 9 | 8 | 14 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | – | 2 | 2 | 2 | 1 | 2 | 1 | 1 | 2 |
Golgi | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 0 |
Mitochondria | 10 | 18 | 39 | 51 | 53 | 108 | 140 | 139 | 181 | 240 | 194 | 269 | 0 | 0 | 0 | – | 11 | 14 | 7 | 11 | 8 | 14 | 20 | 12 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 12 | 16 | 16 | 8 | 11 | 5 | 5 | 9 | 1 | 10 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 2 | 2 | 16 | 5 | 5 | 0 | 4 | 4 | 0 | 2 | 3 | 1 | 13 | 12 | 4 | – | 12 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 2 | – | 2 | 0 | 2 | 6 | 1 | 4 | 8 | 4 |
Unique Cell Count | 213 | 373 | 323 | 214 | 243 | 235 | 340 | 304 | 335 | 438 | 341 | 474 | 755 | 754 | 716 | 249 | 157 | 259 | 440 | 378 | 271 | 345 | 360 | |
Labelled Cell Count | 267 | 516 | 465 | 319 | 354 | 356 | 485 | 451 | 499 | 678 | 516 | 712 | 966 | 963 | 890 | 326 | 213 | 259 | 440 | 378 | 271 | 345 | 360 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.8 | 5.3 | 5.2 | 5.5 | 4.9 | 5.1 | 5.3 | 5.2 | 4.9 | 5.1 | 5.2 | 6.7 | 6.7 | 6.8 | 4.8 | 5.6 | 6.5 | 6.2 | 6.4 | 6.8 |
Std Deviation (1e-4) | 0.8 | 0.7 | 1.1 | 1.2 | 1.5 | 1.1 | 1.3 | 1.1 | 1.0 | 0.9 | 1.1 | 0.7 | 0.8 | 0.8 | 0.9 | 1.0 | 1.2 | 2.2 | 1.0 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.05 | -0.11 | -0.04 | 0.0 | -0.04 | -0.1 | -0.06 | -0.02 | 0.34 | 0.34 | 0.36 | -0.13 | 0.07 | 0.31 | 0.23 | 0.27 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.7 | -1.9 | -2.3 | -4.2 | -2.8 | -3.7 | -3.1 | -1.7 | -5.3 | -8.3 | -8.1 | -8.3 | – | -4.5 | -4.6 |
Bud | 0.8 | -0.9 | -0.8 | -0.7 | 2.2 | 0.4 | 0.7 | 0.2 | -0.3 | -3.2 | -3.2 | -3.8 | – | -3.0 | -1.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -1.3 | -1.5 | -3.3 | -4.1 | -4.8 | -3.9 | -3.2 | -4.4 | -2.5 | 1.7 | 1.8 | -2.4 | – | -3.1 | -2.6 |
Cytoplasm | 1.0 | 1.2 | 0.3 | 0.4 | -0.9 | -1.5 | -1.9 | -2.9 | -3.1 | 5.1 | 5.1 | 9.5 | – | 5.0 | 4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 1.9 | 0 | 0 | 0 | – | 4.3 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 3.6 | 3.1 | 9.0 | 8.4 | 9.3 | 11.4 | 12.1 | 12.1 | 12.7 | -9.7 | -9.7 | -9.5 | – | -3.2 | -1.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 1.9 | 1.6 | -0.2 | -0.3 | -1.6 | -1.8 | -1.4 | -3.2 | -1.4 | 0 | 0 | 0 | – | -3.1 | 0 |
SpindlePole | -1.5 | -1.8 | -3.5 | -2.8 | -2.6 | -4.1 | -4.0 | -3.1 | -4.5 | -3.0 | -3.2 | -4.8 | – | -0.1 | -1.6 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8583 | 3.3442 | 3.1859 | 3.0275 | 2.9249 | 3.1554 | 3.5042 | 4.7827 | 4.833 | 3.9339 | 4.7445 | 4.5247 | 4.643 | 4.8481 | 4.068 | 5.1305 | 4.365 | 4.1476 |
Actin | 0.0518 | 0.007 | 0.0162 | 0.0011 | 0.025 | 0.004 | 0.0157 | 0.0061 | 0.0222 | 0.0001 | 0.0021 | 0.0003 | 0.1388 | 0.0017 | 0.0122 | 0.0004 | 0.0691 | 0.0156 |
Bud | 0.012 | 0.0009 | 0.0102 | 0.0007 | 0.0013 | 0.0022 | 0.0011 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.001 | 0.0005 | 0.0038 | 0.0002 | 0.0006 | 0.001 |
Bud Neck | 0.0092 | 0.0043 | 0.0187 | 0.014 | 0.0044 | 0.0067 | 0.017 | 0.0034 | 0.0063 | 0.0015 | 0.0065 | 0.003 | 0.0068 | 0.0056 | 0.0116 | 0.0014 | 0.0018 | 0.01 |
Bud Periphery | 0.0307 | 0.0067 | 0.014 | 0.0145 | 0.0259 | 0.0127 | 0.0061 | 0.0033 | 0.0066 | 0.0046 | 0.0067 | 0.0059 | 0.0029 | 0.0022 | 0.0322 | 0.0035 | 0.0021 | 0.0108 |
Bud Site | 0.0236 | 0.0729 | 0.0615 | 0.0062 | 0.0036 | 0.0043 | 0.0246 | 0.0903 | 0.0257 | 0.0009 | 0.0036 | 0.001 | 0.0058 | 0.0645 | 0.0589 | 0.0016 | 0.0046 | 0.0036 |
Cell Periphery | 0.4019 | 0.3918 | 0.4425 | 0.6161 | 0.572 | 0.5359 | 0.593 | 0.4598 | 0.4726 | 0.7106 | 0.4463 | 0.6279 | 0.1637 | 0.3432 | 0.497 | 0.3075 | 0.6641 | 0.4503 |
Cytoplasm | 0.2584 | 0.4563 | 0.3631 | 0.3268 | 0.3126 | 0.3673 | 0.2403 | 0.3371 | 0.3284 | 0.2788 | 0.3135 | 0.31 | 0.3847 | 0.4564 | 0.273 | 0.6002 | 0.1847 | 0.4091 |
Cytoplasmic Foci | 0.012 | 0.0016 | 0.0046 | 0.0008 | 0.0039 | 0.0032 | 0.0027 | 0.0012 | 0.0014 | 0 | 0.0012 | 0.0003 | 0.04 | 0.0029 | 0.005 | 0.0016 | 0.0181 | 0.0028 |
Eisosomes | 0.0055 | 0.0073 | 0.0042 | 0.0014 | 0.0021 | 0.0013 | 0.0117 | 0.0042 | 0.0071 | 0.0003 | 0.0073 | 0.0007 | 0.003 | 0.0008 | 0.0022 | 0.0005 | 0.0003 | 0.0011 |
Endoplasmic Reticulum | 0.0146 | 0.0103 | 0.0102 | 0.0073 | 0.0127 | 0.0094 | 0.003 | 0.0028 | 0.0036 | 0.0014 | 0.0111 | 0.0016 | 0.0301 | 0.005 | 0.0077 | 0.009 | 0.0024 | 0.0033 |
Endosome | 0.019 | 0.0003 | 0.0046 | 0.0001 | 0.0044 | 0.0008 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0644 | 0.0097 | 0.0408 | 0.0004 | 0.0092 | 0.0193 |
Golgi | 0.0057 | 0.001 | 0.0036 | 0.0002 | 0.0091 | 0.001 | 0.0011 | 0.0003 | 0.0007 | 0 | 0.0004 | 0.0001 | 0.0135 | 0.0011 | 0.0015 | 0.0002 | 0.0166 | 0.0014 |
Lipid Particles | 0.0067 | 0.0042 | 0.004 | 0.0005 | 0.0116 | 0.0054 | 0.002 | 0.0015 | 0.0008 | 0.0001 | 0.0014 | 0.0004 | 0.0072 | 0.0015 | 0.0016 | 0.0016 | 0.0067 | 0.0021 |
Mitochondria | 0.0053 | 0.0003 | 0.003 | 0.0002 | 0.0016 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0045 | 0.001 | 0.0009 | 0.0001 | 0.003 | 0.0002 |
None | 0.1054 | 0.0323 | 0.028 | 0.0066 | 0.0015 | 0.0363 | 0.0752 | 0.087 | 0.1199 | 0.0004 | 0.1943 | 0.0456 | 0.0216 | 0.0758 | 0.0046 | 0.0653 | 0.0027 | 0.0388 |
Nuclear Periphery | 0.0096 | 0.0003 | 0.0014 | 0.0002 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.009 | 0.0013 | 0.0006 | 0.0003 | 0.0003 | 0.0005 |
Nucleolus | 0.0017 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0006 | 0 | 0.0009 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0079 | 0.0004 | 0.0016 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0 | 0.0007 | 0.0001 | 0.0026 | 0.0012 | 0.0007 | 0.0004 | 0.0002 | 0.0005 |
Peroxisomes | 0.0025 | 0.0001 | 0.0016 | 0 | 0.0031 | 0.0036 | 0.0003 | 0 | 0.0005 | 0 | 0 | 0 | 0.0027 | 0.0001 | 0.0002 | 0 | 0.0106 | 0.0001 |
Punctate Nuclear | 0.0057 | 0.0001 | 0.0023 | 0 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0298 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0002 |
Vacuole | 0.0096 | 0.0018 | 0.0041 | 0.0029 | 0.0034 | 0.0041 | 0.0036 | 0.0016 | 0.0019 | 0.0011 | 0.0031 | 0.0027 | 0.0605 | 0.0242 | 0.0395 | 0.0056 | 0.0016 | 0.0204 |
Vacuole Periphery | 0.0012 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0063 | 0.0007 | 0.0054 | 0 | 0.0001 | 0.0086 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.6628 | 40.9643 | 36.3967 | 45.0153 | 50.4232 | 42.1834 | 42.6451 | 46.7142 | 38.6887 | 52.0269 |
Translational Efficiency | 0.4501 | 0.4918 | 0.4818 | 0.3399 | 0.3447 | 0.4658 | 0.4366 | 0.3209 | 0.3595 | 0.328 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
138 | 395 | 156 | 1145 | 2281 | 1677 | 1961 | 1563 | 2419 | 2072 | 2117 | 2708 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 878.66 | 816.07 | 941.26 | 913.02 | 726.19 | 785.70 | 856.44 | 892.77 | 734.89 | 791.49 | 862.69 | 901.33 |
Standard Deviation | 133.76 | 97.04 | 101.90 | 111.72 | 86.60 | 102.59 | 113.47 | 120.48 | 96.66 | 102.25 | 114.82 | 117.28 |
Intensity Change Log 2 | — | -0.106612 | 0.099288 | 0.055341 | — | 0.113631 | 0.238005 | 0.297941 | — | -0.002771 | 0.163714 | 0.170192 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000410 | 0.025971 | 0.001297 | 0.006492 | 0.008421 | 0.013738 | 0.000922 | 0.004160 | 0.007964 | 0.016070 | 0.000950 | 0.005146 |
Bud Neck | 0.006815 | 0.092365 | 0.004914 | 0.016303 | 0.068897 | 0.094913 | 0.017822 | 0.017107 | 0.065355 | 0.094427 | 0.016871 | 0.016767 |
Bud Site | 0.035361 | 0.068242 | 0.018730 | 0.065990 | 0.027476 | 0.038928 | 0.025713 | 0.052286 | 0.027926 | 0.044517 | 0.025198 | 0.058080 |
Cell Periphery | 0.000534 | 0.081951 | 0.000987 | 0.003361 | 0.036635 | 0.042015 | 0.000995 | 0.001241 | 0.034575 | 0.049628 | 0.000995 | 0.002138 |
Cytoplasm | 0.280429* | 0.456529* | 0.273174* | 0.301221* | 0.582328* | 0.542775* | 0.508218* | 0.410193* | 0.565105* | 0.526333* | 0.490898* | 0.364117* |
Cytoplasmic Foci | 0.087303 | 0.078050 | 0.077732 | 0.043636 | 0.051303 | 0.068217 | 0.038000 | 0.053456 | 0.053356 | 0.070091 | 0.040928 | 0.049304 |
Eisosomes | 0.000242 | 0.051825 | 0.000306 | 0.001434 | 0.069997 | 0.059222 | 0.000266 | 0.000724 | 0.066017 | 0.057812 | 0.000269 | 0.001024 |
Endoplasmic Reticulum | 0.003369 | 0.003798 | 0.012499 | 0.006199 | 0.004498 | 0.003740 | 0.004827 | 0.003486 | 0.004434 | 0.003751 | 0.005393 | 0.004633 |
Endosome | 0.021617 | 0.007648 | 0.016493 | 0.012444 | 0.003351 | 0.007464 | 0.003747 | 0.013296 | 0.004393 | 0.007499 | 0.004686 | 0.012936 |
Golgi | 0.010662 | 0.012531 | 0.000310 | 0.004187 | 0.014321 | 0.016854 | 0.000470 | 0.005816 | 0.014112 | 0.016030 | 0.000458 | 0.005127 |
Lipid Particles | 0.003441 | 0.007033 | 0.009212 | 0.004630 | 0.012007 | 0.010818 | 0.003550 | 0.004111 | 0.011518 | 0.010096 | 0.003967 | 0.004330 |
Mitochondria | 0.000506 | 0.005289 | 0.000662 | 0.002186 | 0.007474 | 0.009984 | 0.000413 | 0.003688 | 0.007076 | 0.009089 | 0.000431 | 0.003053 |
Mitotic Spindle | 0.001107 | 0.000965 | 0.000626 | 0.011845 | 0.000583 | 0.000940 | 0.000768 | 0.010683 | 0.000613 | 0.000944 | 0.000757 | 0.011174 |
None | 0.000465 | 0.000283 | 0.000937 | 0.000465 | 0.002872 | 0.000785 | 0.000800 | 0.000954 | 0.002734 | 0.000689 | 0.000810 | 0.000747 |
Nuclear Periphery | 0.000494 | 0.000078 | 0.001446 | 0.000850 | 0.000114 | 0.000114 | 0.000589 | 0.000297 | 0.000136 | 0.000107 | 0.000652 | 0.000531 |
Nuclear Periphery Foci | 0.001574 | 0.001245 | 0.012769 | 0.003346 | 0.000294 | 0.000264 | 0.002332 | 0.001924 | 0.000367 | 0.000451 | 0.003101 | 0.002525 |
Nucleolus | 0.000260 | 0.000120 | 0.000543 | 0.000154 | 0.000271 | 0.000199 | 0.000210 | 0.000165 | 0.000271 | 0.000184 | 0.000234 | 0.000160 |
Nucleus | 0.100908 | 0.047081 | 0.199754* | 0.056060 | 0.075890 | 0.043407 | 0.196777* | 0.057964 | 0.077317 | 0.044107 | 0.196996* | 0.057159 |
Peroxisomes | 0.001184 | 0.012324 | 0.001549 | 0.004778 | 0.011703 | 0.014775 | 0.000725 | 0.005354 | 0.011103 | 0.014307 | 0.000786 | 0.005110 |
Vacuole | 0.434061* | 0.046178 | 0.365670* | 0.451842* | 0.021201 | 0.029583 | 0.192357 | 0.350716* | 0.044754 | 0.032747 | 0.205128* | 0.393474* |
Vacuole Periphery | 0.009260 | 0.000495 | 0.000389 | 0.002579 | 0.000365 | 0.001267 | 0.000500 | 0.002378 | 0.000873 | 0.001120 | 0.000492 | 0.002463 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.71 | -4.40 | -7.49 | 6.25 | -5.44 | -4.81 | 14.07 | 6.18 | 8.95 | -3.84 | -7.18 | 13.90 | 5.94 | 10.88 | -6.78 |
Bud Neck | -12.25 | 0.46 | -4.08 | 11.41 | -6.70 | -6.26 | 20.11 | 22.09 | 22.30 | 1.70 | -7.68 | 20.11 | 22.80 | 25.38 | 1.86 |
Bud Site | -3.52 | 1.22 | -2.31 | 2.20 | -5.59 | -5.03 | 1.74 | -4.64 | -1.15 | -5.31 | -7.18 | 2.04 | -7.04 | -0.78 | -7.75 |
Cell Periphery | -9.87 | -3.80 | -4.52 | 9.62 | -3.38 | -1.84 | 21.40 | 21.34 | 17.16 | -0.52 | -5.06 | 21.25 | 20.72 | 19.05 | -3.21 |
Cytoplasm | -7.91 | 0.37 | -1.49 | 11.52 | -2.33 | 5.79 | 14.30 | 23.33 | 16.75 | 9.36 | 5.65 | 14.04 | 31.61 | 24.16 | 14.28 |
Cytoplasmic Foci | 0.12 | 1.44 | 5.15 | 6.85 | 3.81 | -5.18 | 5.44 | 1.04 | 5.86 | -4.06 | -5.68 | 5.42 | 4.05 | 9.38 | -1.80 |
Eisosomes | -11.90 | -1.56 | -6.09 | 11.71 | -5.75 | 3.68 | 35.33* | 35.15* | 26.96 | -5.61 | 3.04 | 34.79 | 34.50 | 29.31 | -8.04 |
Endoplasmic Reticulum | -0.97 | -7.21 | -2.24 | -1.97 | 6.49 | 1.01 | -1.06 | 2.51 | 1.03 | 4.11 | 1.00 | -2.39 | 0.95 | -0.27 | 4.03 |
Endosome | 2.63 | 0.60 | 2.55 | -0.55 | 4.06 | -7.39 | -0.85 | -8.08 | -2.88 | -7.70 | -5.39 | -0.86 | -7.78 | -2.55 | -7.43 |
Golgi | -0.90 | 1.48 | 1.01 | 5.47 | -3.21 | -2.47 | 20.27 | 9.12 | 10.51 | -3.90 | -2.12 | 19.16 | 11.52 | 13.32 | -4.76 |
Lipid Particles | -4.66 | -6.38 | -1.08 | 4.77 | 6.66 | 1.62 | 14.37 | 13.02 | 11.63 | 0.01 | 2.12 | 13.49 | 13.90 | 13.08 | 0.85 |
Mitochondria | -4.03 | -0.47 | -2.18 | 2.99 | -1.76 | -2.12 | 11.83 | 4.99 | 5.74 | -2.95 | -1.96 | 11.78 | 6.65 | 7.04 | -3.64 |
Mitotic Spindle | 0.44 | 0.66 | -4.03 | -4.12 | -4.54 | -0.86 | -0.67 | -5.01 | -4.79 | -4.92 | -0.78 | -0.56 | -6.81 | -6.50 | -6.74 |
None | 0.75 | -2.54 | -1.08 | -2.30 | 1.59 | 6.93 | 7.25 | 4.91 | -0.28 | -0.34 | 7.29 | 7.14 | 6.22 | -0.27 | 0.17 |
Nuclear Periphery | 6.12 | -8.88 | -1.09 | -2.63 | 2.67 | -0.01 | -18.47 | -8.60 | -7.61 | 6.82 | 1.89 | -20.28 | -3.59 | -3.81 | 1.50 |
Nuclear Periphery Foci | 0.48 | -10.14 | -2.47 | -1.82 | 9.46 | 1.29 | -12.01 | -9.76 | -10.00 | 2.76 | -0.48 | -14.69 | -13.35 | -8.52 | 4.09 |
Nucleolus | 2.81 | -3.51 | 2.48 | -0.73 | 4.94 | 3.49 | 1.54 | 6.23 | 1.88 | 2.43 | 4.61 | 0.67 | 7.49 | 2.04 | 3.96 |
Nucleus | 2.91 | -5.88 | 1.68 | -3.37 | 8.35 | 12.57 | -26.36 | 4.23 | -6.56 | 28.03 | 12.82 | -26.98 | 4.96 | -7.86 | 30.39 |
Peroxisomes | -7.78 | -0.55 | -2.67 | 5.57 | -2.26 | -2.85 | 17.80 | 7.55 | 8.84 | -4.97 | -3.33 | 17.68 | 9.03 | 10.91 | -5.79 |
Vacuole | 16.04 | 2.81 | 0.13 | -47.09 | -4.13 | -5.89 | -39.71 | -50.73 | -49.05 | -19.39 | 5.01 | -32.47 | -60.89 | -69.08* | -26.60 |
Vacuole Periphery | 1.31 | 1.34 | 1.07 | -2.24 | -2.52 | -2.15 | -0.22 | -2.82 | -0.66 | -2.52 | -0.90 | 0.99 | -2.13 | -1.12 | -3.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|