Standard name
Human Ortholog
Description Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0.09 0.08 0.14 0.09 0.07 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.05 0.07 0.15 0.14 0.13 0.17 0 0 0.05 0.05 0 0.09 0 0 0 0 0 0
Nucleus 0.94 0.95 0.87 0.94 0.84 0.81 0.78 0.73 0.73 0.74 0.74 0.71 0.82 0.8 0.7 0.84 0.88 0.82 0.91 0.92 0.9 0.84 0.79 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.09 0.11 0.14 0.23 0.42 0.34 0.38 0.54 0.61 0.64 0.53 0.18 0.32 0.3 0.06 0 0.07 0 0 0 0.09 0.06 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Neck 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 0 1 1 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 7 11 6 14 8 17 21 9 4 3 8 9 15 20 34 19 22 2 2 0 0 0 1
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5 3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 3 0 1 0 0 0 0 0 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 2 0 2 1 7 41 20 33 53 44 21 36 2 7 7 19 7 20 0 2 0 0 0 0
Nucleus 150 209 147 173 216 279 317 351 252 230 115 151 81 149 99 318 234 175 154 205 149 55 117 76
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 13 20 19 25 59 145 138 184 186 189 100 113 18 60 43 23 10 14 4 2 7 5 8 10
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 3 3 3 1 2 2 1 3 3 0 0 3 3 2 2 9 7 7 5 3 7 1 19 23
Unique Cell Count 159 220 169 185 257 344 405 481 347 312 156 213 99 187 141 380 266 213 170 224 167 66 149 119
Labelled Cell Count 173 241 182 207 298 475 497 593 504 468 239 314 115 236 173 409 282 245 170 224 167 66 149 119


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 54.6 64.6 43.2 44.0 42.2 36.6 34.8 35.3 33.8 31.1 30.4 30.4 62.2 63.6 56.6 53.8 63.1 63.1 50.5 50.9 53.8
Std Deviation (1e-4) 15.5 25.6 18.3 16.2 18.1 13.5 18.3 15.2 14.0 11.4 11.6 15.0 29.7 26.8 31.0 24.1 26.5 27.7 18.0 19.6 20.9
Intensity Change (Log2) – – – 0.03 -0.03 -0.24 -0.31 -0.29 -0.35 -0.47 -0.51 -0.51 0.53 0.56 0.39 0.32 0.55 0.55 0.23 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -0.5 -2.4 -1.2 -1.1 -2.2 -3.1 -2.0 -1.2 0.8 0.5 2.2 1.0 0.3 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 2.1 2.8 4.9 4.6 4.3 5.1 0 0 0 2.2 0 3.4
Nucleus 2.1 -0.8 -1.7 -2.4 -3.7 -3.7 -3.4 -3.0 -3.8 -1.1 -1.8 -3.6 -1.0 0.3 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.6 3.1 7.1 5.6 6.5 9.2 10.4 9.9 8.5 1.6 4.7 4.2 -2.1 -3.0 -1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.9906 61.0916 55.822 49.1246 48.7254 52.7928 54.0228 57.4017 51.4978 49.6709 48.4401 48.3967 61.6268 65.7784 62.3595 61.9401 58.684 60.2761
Actin 0.0002 0 0 0 0.0001 0 0.0004 0 0.0014 0 0.0074 0 0.0277 0 0.0091 0 0.0053 0.0034
Bud 0 0 0 0 0.0002 0 0.0007 0 0.0002 0.0008 0.0005 0.0002 0.0009 0 0.0004 0 0.0001 0
Bud Neck 0.0001 0 0 0 0.0002 0 0.0002 0 0.0004 0 0.0001 0.0002 0.0003 0 0.0002 0 0.0001 0.0001
Bud Periphery 0 0 0 0 0.0005 0 0.0006 0 0.0002 0.0003 0.0005 0.0001 0.0005 0 0.0003 0 0.0001 0
Bud Site 0.0001 0 0 0 0.0001 0 0.0007 0 0.0055 0.0003 0.0002 0 0.0013 0 0.0008 0 0.0008 0
Cell Periphery 0 0 0 0 0.0022 0 0.0024 0 0.0001 0.0005 0.0005 0.0005 0.0003 0 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0131 0 0 0 0 0 0.0019 0 0.0019 0.0002 0.001 0.0015 0.0004 0 0.0009 0 0.0001 0.0001
Cytoplasmic Foci 0 0 0 0 0 0 0.0013 0 0.0118 0.0035 0.0004 0.0215 0.0036 0 0.0028 0 0.0126 0.0009
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0012 0 0 0 0 0 0.0005 0 0.0004 0 0.0006 0 0.0005 0 0.0001 0 0 0.0001
Endosome 0.0008 0 0 0 0.0001 0 0.0023 0 0.0065 0 0.003 0 0.0059 0 0.0042 0 0.002 0.0013
Golgi 0 0 0 0 0 0 0.0001 0 0.0022 0 0.0004 0 0.013 0 0.0074 0 0.0031 0.0012
Lipid Particles 0 0 0 0 0.0002 0 0.0011 0 0.0037 0.0003 0.0003 0.0014 0.0045 0 0.0008 0 0.0159 0.005
Mitochondria 0.0021 0.0008 0.0006 0.0004 0.0605 0.0005 0.0091 0.0006 0.0022 0.0421 0.0244 0.0014 0.0227 0.0005 0.0063 0.0017 0.0064 0.0008
None 0.0011 0 0 0 0.0001 0 0.0012 0 0.0006 0.0012 0.0009 0.0015 0.0011 0 0.0005 0 0.0003 0.0001
Nuclear Periphery 0.0643 0.001 0.0002 0.0005 0.0001 0.0001 0.035 0.0006 0.0013 0.0002 0.0572 0.0012 0.0004 0.0003 0.0004 0.0003 0.0002 0.0009
Nucleolus 0.0132 0.022 0.0123 0.0191 0.4766 0.059 0.1068 0.0185 0.0413 0.0251 0.3812 0.0533 0.041 0.0225 0.0183 0.0396 0.1932 0.039
Nucleus 0.8998 0.9759 0.9869 0.9798 0.4486 0.9401 0.8095 0.9801 0.9125 0.921 0.5097 0.9152 0.8707 0.9766 0.9437 0.9576 0.6797 0.9429
Peroxisomes 0 0 0 0 0.0001 0 0.0002 0 0.0016 0.001 0.0006 0.0002 0.0032 0 0.0016 0 0.0791 0.0026
Punctate Nuclear 0.0009 0 0 0 0 0 0.0008 0 0.0055 0 0.0069 0.0003 0.0003 0.0001 0.0002 0 0.0005 0.0011
Vacuole 0.0016 0 0 0 0.0035 0.0001 0.0124 0 0.0006 0.0012 0.0008 0.0008 0.0008 0 0.0009 0.0001 0.0001 0.0001
Vacuole Periphery 0.0012 0.0001 0 0.0001 0.0067 0.0001 0.0126 0.0001 0.0002 0.0021 0.0032 0.0006 0.0008 0 0.0008 0.0004 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 142.652 118.611 74.7895 84.2152 98.4939 131.2359 73.3235 91.8472 76.622 114.1451
Translational Efficiency 2.2521 2.584 2.3989 1.9406 2.6462 2.662 2.315 1.698 2.1569 2.144

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1389 818 1852 712 221 1455 1846 11 1610 2273 3698 723

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4077.90 3700.06 4081.83 5119.60 3671.32 3581.69 4668.89 6555.53 4022.09 3624.29 4374.88 5141.45
Standard Deviation 1155.49 1191.32 971.36 1449.65 779.99 1051.80 1130.56 790.48 1120.25 1105.50 1093.96 1452.55
Intensity Change Log 2 — -0.140278 0.001390 0.328205 — -0.035658 0.346781 0.836414 — -0.089766 0.175351 0.591316

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000400 0.000457 0.000431 0.000565 0.000315 0.000397 0.000440 0.000696 0.000388 0.000418 0.000436 0.000567
Bud Neck 0.001462 0.001392 0.004628 0.004366 0.001357 0.001865 0.002916 0.004420 0.001447 0.001695 0.003774 0.004367
Bud Site 0.000159 0.000344 0.000409 0.000779 0.000141 0.000330 0.000273 0.000264 0.000156 0.000335 0.000341 0.000771
Cell Periphery 0.000223 0.000226 0.000051 0.000163 0.000466 0.000194 0.000090 0.000018 0.000257 0.000206 0.000070 0.000161
Cytoplasm 0.000513 0.000293 0.000081 0.000399 0.000513 0.000190 0.000046 0.000018 0.000513 0.000227 0.000063 0.000393
Cytoplasmic Foci 0.000381 0.001290 0.000239 0.002121 0.000290 0.000635 0.000145 0.000262 0.000368 0.000871 0.000192 0.002092
Eisosomes 0.000273 0.000596 0.000215 0.000126 0.000284 0.000307 0.000239 0.000166 0.000274 0.000411 0.000227 0.000127
Endoplasmic Reticulum 0.000175 0.000242 0.000163 0.000184 0.000179 0.000255 0.000238 0.000135 0.000176 0.000250 0.000201 0.000184
Endosome 0.000005 0.000214 0.000002 0.001402 0.000002 0.000220 0.000095 0.000003 0.000005 0.000218 0.000049 0.001381
Golgi 0.000013 0.000258 0.000012 0.001117 0.000008 0.000081 0.000301 0.000046 0.000012 0.000145 0.000156 0.001101
Lipid Particles 0.000003 0.000056 0.000001 0.000059 0.000001 0.000027 0.000013 0.000003 0.000003 0.000037 0.000007 0.000058
Mitochondria 0.000812 0.000935 0.000960 0.002586 0.000499 0.001027 0.000859 0.002519 0.000769 0.000994 0.000910 0.002585
Mitotic Spindle 0.000005 0.000637 0.000019 0.003929 0.000007 0.000432 0.000029 0.000014 0.000005 0.000506 0.000024 0.003870
None 0.000704 0.000990 0.000430 0.000847 0.000623 0.001509 0.000263 0.000370 0.000693 0.001322 0.000346 0.000840
Nuclear Periphery 0.000027 0.000043 0.000027 0.000285 0.000014 0.000032 0.000019 0.000040 0.000025 0.000036 0.000023 0.000281
Nuclear Periphery Foci 0.000008 0.000552 0.000001 0.000269 0.000001 0.000034 0.000005 0.000001 0.000007 0.000220 0.000003 0.000264
Nucleolus 0.008198 0.009083 0.006884 0.012060 0.005118 0.007819 0.005218 0.027411 0.007776 0.008274 0.006052 0.012293
Nucleus 0.984390* 0.979561* 0.983595* 0.962731* 0.987527* 0.982309* 0.987033* 0.957223* 0.984821* 0.981320* 0.985311* 0.962648*
Peroxisomes 0.001052 0.001363 0.001614 0.002780 0.001568 0.000785 0.001371 0.006004 0.001123 0.000993 0.001493 0.002829
Vacuole 0.000748 0.001031 0.000157 0.001622 0.000923 0.001007 0.000312 0.000240 0.000772 0.001016 0.000235 0.001601
Vacuole Periphery 0.000450 0.000435 0.000082 0.001608 0.000163 0.000546 0.000093 0.000148 0.000411 0.000506 0.000087 0.001586

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.01 -2.33 -5.86 -2.24 -4.94 NaN NaN NaN NaN NaN -1.10 -3.29 -6.44 -4.65 -5.04
Bud Neck 0.44 -17.55 -8.84 -8.81 -0.82 NaN NaN NaN NaN NaN -2.00 -17.79 -9.05 -8.05 -3.09
Bud Site -1.62 -6.50 -3.87 -2.79 -2.73 NaN NaN NaN NaN NaN -2.51 -4.87 -3.90 -2.83 -3.06
Cell Periphery -0.04 1.84 0.43 0.47 -1.98 NaN NaN NaN NaN NaN 0.45 1.91 0.73 0.46 -1.66
Cytoplasm 0.62 1.39 0.14 -0.95 -3.30 NaN NaN NaN NaN NaN 1.02 1.66 0.20 -1.81 -3.50
Cytoplasmic Foci -1.38 0.77 -2.52 -0.85 -2.88 NaN NaN NaN NaN NaN -1.59 1.07 -2.57 -1.68 -2.96
Eisosomes -1.18 1.44 3.57 1.72 5.50 NaN NaN NaN NaN NaN -1.28 1.28 3.94 2.72 6.19
Endoplasmic Reticulum -1.10 0.33 -0.32 0.78 -0.65 NaN NaN NaN NaN NaN -1.70 -0.87 -0.31 1.15 0.26
Endosome -1.82 1.08 -2.15 -1.75 -2.16 NaN NaN NaN NaN NaN -2.17 -0.93 -2.16 -1.76 -2.08
Golgi -1.28 0.08 -1.71 -1.23 -1.71 NaN NaN NaN NaN NaN -1.79 -0.98 -1.71 -1.47 -1.43
Lipid Particles -1.72 1.05 -1.31 0.05 -1.39 NaN NaN NaN NaN NaN -2.20 -0.53 -1.32 -0.40 -1.22
Mitochondria -0.70 -2.14 -3.26 -2.93 -2.96 NaN NaN NaN NaN NaN -1.16 -2.27 -3.39 -2.84 -3.12
Mitotic Spindle -1.37 -1.67 -2.74 -2.21 -2.72 NaN NaN NaN NaN NaN -1.99 -1.72 -2.73 -2.36 -2.72
None -1.05 1.93 -0.85 0.35 -2.29 NaN NaN NaN NaN NaN -1.52 3.18 -0.90 1.01 -2.90
Nuclear Periphery -1.15 -0.13 -2.66 -2.45 -2.65 NaN NaN NaN NaN NaN -1.55 0.35 -2.68 -2.54 -2.70
Nuclear Periphery Foci -1.30 1.67 -1.43 0.71 -1.47 NaN NaN NaN NaN NaN -1.40 0.99 -1.43 -0.05 -1.46
Nucleolus -0.56 1.00 -2.45 -2.01 -3.02 NaN NaN NaN NaN NaN -0.55 1.79 -2.83 -2.57 -3.65
Nucleus 1.62 0.81 4.83 3.57 4.69 NaN NaN NaN NaN NaN 1.92 -0.15 5.05 4.24 5.21
Peroxisomes -0.99 -1.95 -4.03 -3.40 -2.61 NaN NaN NaN NaN NaN 0.58 -1.77 -4.11 -4.55 -3.29
Vacuole -0.90 3.49 -1.28 -0.77 -2.35 NaN NaN NaN NaN NaN -0.88 3.33 -1.24 -0.81 -2.22
Vacuole Periphery 0.08 1.86 -1.56 -1.63 -2.23 NaN NaN NaN NaN NaN -0.40 1.89 -1.64 -1.51 -2.22
Standard name
Human Ortholog
Description Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Hrp1

Hrp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hrp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available